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The map label for this gene is amt [H]

Identifier: 13473594

GI number: 13473594

Start: 3387960

End: 3389315

Strand: Reverse

Name: amt [H]

Synonym: mll4246

Alternate gene names: 13473594

Gene position: 3389315-3387960 (Counterclockwise)

Preceding gene: 13473595

Following gene: 13473593

Centisome position: 48.17

GC content: 64.75

Gene sequence:

>1356_bases
ATGAATATTTCTTCCACCTTGAAGACAACGGGACGGGCGGCCCTGCTGGGCTCGCTTGCTCTGGCAGCATTGGGCAGCGT
CGCCGCCTTTGCGCAGGAAGCCGCTCCGGCCGCTGCCGCAGCCGCCCCGGCTGCCGCTCCCACCCCGGTGCTCGACACCG
GCAACACCGCCTGGATGCTGACCTCGACGGCCCTGGTGCTGATGATGACCATTCCGGGCCTGGCGCTGTTCTACGGCGGC
ATGGTGCGCAAGAAGAACGTGCTCGCCACCATCATGCAGAGCTTCGCCATCACCTGCCTGGTGACGGTGCTGTGGTTCAT
GTTCGGCTATTCGCTGGCCTTCTCCGACGGCGGCGGCATGAATGCCTATCTCGGCGGCTTCTCGAAATTCTTCCATCACG
GCATCACGGTCTCGACGCTCTGGCTGCCGGGGGTCTCGAACATTCCTGAATTCGTCTTCTCGATGTTCCAGATGACCTTC
GCCATCATCACACCTGCCCTCATCGCCGGCGCCTTCGCCGAGCGCATGAAGTTCTCGGCGCTGCTGCTGTTCATGGCGCT
GTGGTTGATCGTGGTCTATTGCCCGATCGCACATTGGGTCTGGGGTGGCGGCTTCCTCGGCACGGCTGGCGTGCTCGACT
TCGCCGGCGGTACGGTCGTCCACATCAACGCCGGTGTCGCGGGCCTGGTCTGCGCGCTGGTTCTTGGCAAGCGCGAAGGC
TACGGCACCACCAACATGGCGCCGCACAACCTGGTCTATTCGGTGATCGGCGCTTCGCTGCTGTGGGTCGGCTGGTTCGG
CTTCAACGCCGGTTCGGAACTGGCGGCGGACGGTCTTGCCGGCGCTGCGATGATGAACACGCAGGTCGCCACCGCTGCCG
CCGCGCTCGCCTGGATGTTCGCTGAATGGATCGTCGCCAAGAAGCCCTCGGTGCTCGGCATCATCTCGGGTGCCGTCGCC
GGCCTCGTCGCGGTGACGCCGGCTTCCGGCTTCGTCAACCCGACCGGCGCTTTCATCATCGGCATCGTTGCCGGCGTGCT
CTGCTACATCTCGGCGGTCAAGGTGAAGCACATGTTCGGCTATGACGACTCGCTCGATGCCTTCGGCGTGCACGGCGTCG
GCGGCATCATCGGTGCGCTGCTCACCGGCGCGCTGGCCGATCCGGCGATCAACGCGCTCGGCAAGGGCGGCTCTGTGGTC
ACCCAGCTCTACGGCATCGTCTTCACCATCCTGTGGACGGCGATCGCCACCTTCGTGATCCTCTACATCGTCAAGGCGCT
GGTCGGCCTGCGTCCGACGACCCAGGAAGAGGTCGAAGGCCTCGACATCTCCCAGCATGGCGAAGTGGTGCCGTAA

Upstream 100 bases:

>100_bases
ATCTTCGTTTTCGGCATCGATCAGGCGGTGCGCATCCGCACCGGCGAAACAGACACCGACGCGCTCTGAGCGGCGAACAC
GTATTCCAATGGAGAGTTCA

Downstream 100 bases:

>100_bases
GGCCAGCCTCGCCAACGGGGCCGGCGGATCCTCCTCCCGCCGGCCCCATTGTCCCTCACGCAATTCCCGGGAGAATCGCA
ATCCGGCTCTCCCAGGGACT

Product: ammonium transporter AmtB

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNISSTLKTTGRAALLGSLALAALGSVAAFAQEAAPAAAAAAPAAAPTPVLDTGNTAWMLTSTALVLMMTIPGLALFYGG
MVRKKNVLATIMQSFAITCLVTVLWFMFGYSLAFSDGGGMNAYLGGFSKFFHHGITVSTLWLPGVSNIPEFVFSMFQMTF
AIITPALIAGAFAERMKFSALLLFMALWLIVVYCPIAHWVWGGGFLGTAGVLDFAGGTVVHINAGVAGLVCALVLGKREG
YGTTNMAPHNLVYSVIGASLLWVGWFGFNAGSELAADGLAGAAMMNTQVATAAAALAWMFAEWIVAKKPSVLGIISGAVA
GLVAVTPASGFVNPTGAFIIGIVAGVLCYISAVKVKHMFGYDDSLDAFGVHGVGGIIGALLTGALADPAINALGKGGSVV
TQLYGIVFTILWTAIATFVILYIVKALVGLRPTTQEEVEGLDISQHGEVVP

Sequences:

>Translated_451_residues
MNISSTLKTTGRAALLGSLALAALGSVAAFAQEAAPAAAAAAPAAAPTPVLDTGNTAWMLTSTALVLMMTIPGLALFYGG
MVRKKNVLATIMQSFAITCLVTVLWFMFGYSLAFSDGGGMNAYLGGFSKFFHHGITVSTLWLPGVSNIPEFVFSMFQMTF
AIITPALIAGAFAERMKFSALLLFMALWLIVVYCPIAHWVWGGGFLGTAGVLDFAGGTVVHINAGVAGLVCALVLGKREG
YGTTNMAPHNLVYSVIGASLLWVGWFGFNAGSELAADGLAGAAMMNTQVATAAAALAWMFAEWIVAKKPSVLGIISGAVA
GLVAVTPASGFVNPTGAFIIGIVAGVLCYISAVKVKHMFGYDDSLDAFGVHGVGGIIGALLTGALADPAINALGKGGSVV
TQLYGIVFTILWTAIATFVILYIVKALVGLRPTTQEEVEGLDISQHGEVVP
>Mature_451_residues
MNISSTLKTTGRAALLGSLALAALGSVAAFAQEAAPAAAAAAPAAAPTPVLDTGNTAWMLTSTALVLMMTIPGLALFYGG
MVRKKNVLATIMQSFAITCLVTVLWFMFGYSLAFSDGGGMNAYLGGFSKFFHHGITVSTLWLPGVSNIPEFVFSMFQMTF
AIITPALIAGAFAERMKFSALLLFMALWLIVVYCPIAHWVWGGGFLGTAGVLDFAGGTVVHINAGVAGLVCALVLGKREG
YGTTNMAPHNLVYSVIGASLLWVGWFGFNAGSELAADGLAGAAMMNTQVATAAAALAWMFAEWIVAKKPSVLGIISGAVA
GLVAVTPASGFVNPTGAFIIGIVAGVLCYISAVKVKHMFGYDDSLDAFGVHGVGGIIGALLTGALADPAINALGKGGSVV
TQLYGIVFTILWTAIATFVILYIVKALVGLRPTTQEEVEGLDISQHGEVVP

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=419, Percent_Identity=46.3007159904535, Blast_Score=353, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI17550182, Length=436, Percent_Identity=27.2935779816514, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17550180, Length=432, Percent_Identity=27.5462962962963, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI133931100, Length=435, Percent_Identity=26.6666666666667, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32564042, Length=431, Percent_Identity=25.754060324826, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324187, Length=420, Percent_Identity=37.1428571428571, Blast_Score=243, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6325396, Length=416, Percent_Identity=35.0961538461538, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6321559, Length=416, Percent_Identity=34.8557692307692, Blast_Score=221, Evalue=2e-58,
Organism=Drosophila melanogaster, GI161078314, Length=421, Percent_Identity=28.978622327791, Blast_Score=136, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 46501; Mature: 46501

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNISSTLKTTGRAALLGSLALAALGSVAAFAQEAAPAAAAAAPAAAPTPVLDTGNTAWML
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEECCCCEEHH
TSTALVLMMTIPGLALFYGGMVRKKNVLATIMQSFAITCLVTVLWFMFGYSLAFSDGGGM
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCC
NAYLGGFSKFFHHGITVSTLWLPGVSNIPEFVFSMFQMTFAIITPALIAGAFAERMKFSA
HHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLFMALWLIVVYCPIAHWVWGGGFLGTAGVLDFAGGTVVHINAGVAGLVCALVLGKREG
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCC
YGTTNMAPHNLVYSVIGASLLWVGWFGFNAGSELAADGLAGAAMMNTQVATAAAALAWMF
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEWIVAKKPSVLGIISGAVAGLVAVTPASGFVNPTGAFIIGIVAGVLCYISAVKVKHMFG
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
YDDSLDAFGVHGVGGIIGALLTGALADPAINALGKGGSVVTQLYGIVFTILWTAIATFVI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LYIVKALVGLRPTTQEEVEGLDISQHGEVVP
HHHHHHHHCCCCCCHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNISSTLKTTGRAALLGSLALAALGSVAAFAQEAAPAAAAAAPAAAPTPVLDTGNTAWML
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEECCCCEEHH
TSTALVLMMTIPGLALFYGGMVRKKNVLATIMQSFAITCLVTVLWFMFGYSLAFSDGGGM
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCC
NAYLGGFSKFFHHGITVSTLWLPGVSNIPEFVFSMFQMTFAIITPALIAGAFAERMKFSA
HHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLFMALWLIVVYCPIAHWVWGGGFLGTAGVLDFAGGTVVHINAGVAGLVCALVLGKREG
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCC
YGTTNMAPHNLVYSVIGASLLWVGWFGFNAGSELAADGLAGAAMMNTQVATAAAALAWMF
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEWIVAKKPSVLGIISGAVAGLVAVTPASGFVNPTGAFIIGIVAGVLCYISAVKVKHMFG
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
YDDSLDAFGVHGVGGIIGALLTGALADPAINALGKGGSVVTQLYGIVFTILWTAIATFVI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LYIVKALVGLRPTTQEEVEGLDISQHGEVVP
HHHHHHHHCCCCCCHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]