Click here to switch to the map view.

The map label for this gene is xthA [H]

Identifier: 13473590

GI number: 13473590

Start: 3382149

End: 3382955

Strand: Reverse

Name: xthA [H]

Synonym: mll4241

Alternate gene names: 13473590

Gene position: 3382955-3382149 (Counterclockwise)

Preceding gene: 13473591

Following gene: 13473587

Centisome position: 48.08

GC content: 61.83

Gene sequence:

>807_bases
ATGCCCTTTTCCATCGCCACCTGGAACATCAACTCCGTGCGCCTGCGTATGCCGATCGTCGAGCGGCTGCTCGATGAATA
CGCCCCCGATGTGCTCTGCTTGCAGGAAACGAAAGTGCCCGATGAGCTGTTTCCCGAAAAGGCATTCCGCAGGCTTGGCT
ACCAGTACATCGCCTTTCATGGCCAGAAGGGCTATCACGGCGTCGCCACGGTGGCGCGGCGGCCGATCGAGGTGGTCGAA
AAGCGCCGCTTCTGCGACATCGAGGACAGCCGGCATCTGTCGGTGACGGTGCGCGCCGGCGGCAAGACGATCCTGCTGCA
CAATTTCTACGTTCCGGCGGGTGGCGACGAGCCCGATCCCGAAATCAACAAGAAATTCAAGCACAAGCTCGACTTTGTCG
CCGAGATGAACGCCATCCGCGCCGAGCACACAGAGGTGTCTGCCTCGGTGCTGGTCGGCGACCTCAACATCGCGCCGCTC
GAGCACGACGTCTGGTCGCACAAGCAATTGCTCAACGTGGTCAGCCACACGCCGGTCGAGACCGAAAACTTCGAGGCGAT
GCGGCTCGCCGGCAATTGGGTCGACCTGATGCGGCACAACGTGCCATTGGACCAGAAGCTCTACACTTGGTGGAGTTATC
GCGCACAGGATTGGGAGCTGTCCAATCGCGGCCGGCGGCTCGACCATGTCTGGTCGTCGCCCAATCTGGTGCAGGATTTC
GCCGGCTACGAAATCCTGCGGGCGGCGCGCGGCTGGGAGCGGCCGTCGGACCATGTTCCCGTCATCGCACGGTTCGATCT
GGATTAG

Upstream 100 bases:

>100_bases
GCTGCCTGCGGCTGGCAGTTTCCGGACCGACCCCTCAAAAGGCGTCTTCTCCGTCAGGACCTATCGATCCTGGCTGGCTG
CACGCTGTCCGGATTGCCAA

Downstream 100 bases:

>100_bases
GTGTGCCGAAGCGCCCGCCAATTCGTCATGCTATAGCGGAGCGACGCGAAGCGGAGCGCAGACCCCTGGAGCTGCTCCGC
AGCCCAAGGGGTCTGCGTGA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MPFSIATWNINSVRLRMPIVERLLDEYAPDVLCLQETKVPDELFPEKAFRRLGYQYIAFHGQKGYHGVATVARRPIEVVE
KRRFCDIEDSRHLSVTVRAGGKTILLHNFYVPAGGDEPDPEINKKFKHKLDFVAEMNAIRAEHTEVSASVLVGDLNIAPL
EHDVWSHKQLLNVVSHTPVETENFEAMRLAGNWVDLMRHNVPLDQKLYTWWSYRAQDWELSNRGRRLDHVWSSPNLVQDF
AGYEILRAARGWERPSDHVPVIARFDLD

Sequences:

>Translated_268_residues
MPFSIATWNINSVRLRMPIVERLLDEYAPDVLCLQETKVPDELFPEKAFRRLGYQYIAFHGQKGYHGVATVARRPIEVVE
KRRFCDIEDSRHLSVTVRAGGKTILLHNFYVPAGGDEPDPEINKKFKHKLDFVAEMNAIRAEHTEVSASVLVGDLNIAPL
EHDVWSHKQLLNVVSHTPVETENFEAMRLAGNWVDLMRHNVPLDQKLYTWWSYRAQDWELSNRGRRLDHVWSSPNLVQDF
AGYEILRAARGWERPSDHVPVIARFDLD
>Mature_267_residues
PFSIATWNINSVRLRMPIVERLLDEYAPDVLCLQETKVPDELFPEKAFRRLGYQYIAFHGQKGYHGVATVARRPIEVVEK
RRFCDIEDSRHLSVTVRAGGKTILLHNFYVPAGGDEPDPEINKKFKHKLDFVAEMNAIRAEHTEVSASVLVGDLNIAPLE
HDVWSHKQLLNVVSHTPVETENFEAMRLAGNWVDLMRHNVPLDQKLYTWWSYRAQDWELSNRGRRLDHVWSSPNLVQDFA
GYEILRAARGWERPSDHVPVIARFDLD

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=272, Percent_Identity=30.1470588235294, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI18375503, Length=272, Percent_Identity=30.1470588235294, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI18375501, Length=272, Percent_Identity=30.1470588235294, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1788046, Length=274, Percent_Identity=31.3868613138686, Blast_Score=111, Evalue=5e-26,
Organism=Drosophila melanogaster, GI221330655, Length=276, Percent_Identity=27.8985507246377, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI17136678, Length=276, Percent_Identity=27.8985507246377, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31034; Mature: 30902

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFSIATWNINSVRLRMPIVERLLDEYAPDVLCLQETKVPDELFPEKAFRRLGYQYIAFH
CCCEEEEECCCCEEEECHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEE
GQKGYHGVATVARRPIEVVEKRRFCDIEDSRHLSVTVRAGGKTILLHNFYVPAGGDEPDP
CCCCCCHHHHHHHCCHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEEEEECCCCCCCCH
EINKKFKHKLDFVAEMNAIRAEHTEVSASVLVGDLNIAPLEHDVWSHKQLLNVVSHTPVE
HHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCC
TENFEAMRLAGNWVDLMRHNVPLDQKLYTWWSYRAQDWELSNRGRRLDHVWSSPNLVQDF
CCCCCEEEECCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCEEHHHCCCCCHHHHH
AGYEILRAARGWERPSDHVPVIARFDLD
HHHHHHHHHHCCCCCCCCCCEEEEECCC
>Mature Secondary Structure 
PFSIATWNINSVRLRMPIVERLLDEYAPDVLCLQETKVPDELFPEKAFRRLGYQYIAFH
CCEEEEECCCCEEEECHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEE
GQKGYHGVATVARRPIEVVEKRRFCDIEDSRHLSVTVRAGGKTILLHNFYVPAGGDEPDP
CCCCCCHHHHHHHCCHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEEEEECCCCCCCCH
EINKKFKHKLDFVAEMNAIRAEHTEVSASVLVGDLNIAPLEHDVWSHKQLLNVVSHTPVE
HHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCC
TENFEAMRLAGNWVDLMRHNVPLDQKLYTWWSYRAQDWELSNRGRRLDHVWSSPNLVQDF
CCCCCEEEECCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCEEHHHCCCCCHHHHH
AGYEILRAARGWERPSDHVPVIARFDLD
HHHHHHHHHHCCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]