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The map label for this gene is cga [H]

Identifier: 13473562

GI number: 13473562

Start: 3358246

End: 3360660

Strand: Direct

Name: cga [H]

Synonym: mlr4205

Alternate gene names: 13473562

Gene position: 3358246-3360660 (Clockwise)

Preceding gene: 13473556

Following gene: 13473563

Centisome position: 47.73

GC content: 66.71

Gene sequence:

>2415_bases
ATGGCAGTGACGCCGATCGTTCCCCCGGGCGCACCGGGCATTCCGGCGCGCTGGACATCGAGCGCCAAGAGCGGCGTCGG
GACGTCGCTTTCGCCGGCCGGCCACATCTGGTTCACCATCAGCCACGGCATCCTCGAAGTCTACTATCCACGGCTCGACA
GCGCCTGCACGCGCGATCTCGGCCTGATCGTCACCGGTCCCGGCGGCTATTTCTCCGAAGAGAAGCGCGATGCCGCGCAT
GAAATCGAGCCCTTCGAGGCCGGCGTGCCCGCCTACCGGCTGGTCAACACGGCCGGCGACGGCGCCTACCGCATCGAGAA
GCGCATCCTCTGCGACCCTGCGCGGCCCGTGCTGCTGCAGGAAATCACCTTCACGGCGCTTAAAGGTGCGCCCGACGATT
ACCGCGTCTACGCGCTTCTGGCGCCGCATCTGGTCAATGCCGGCATGGGCAACACCGCCTGGGTCGGCGACCACAAGGGA
AAGCCCGTGCTGTTCGCCTCGGGCCGCGGCTCTTGCCTGGCGCTGGCCTCGTCGCTGCCCTGGCGGGACTGTTCAGCCGG
CTATGTCGGCTTCTCCGACGGCTGGCAGCAGCTCAATCATACCGGCATCCTTGATCAGGCTTGCCAACGGGCGGAAGACG
GTAATGTCGCACTGACCGGCGAGATCGGCTTTACATCAACAACGACAAAAGCCGTGCTGGCGCTGGGTTTCGGCGCGACA
CCGGACGAGGCCGCCGAAAATGCCTTCGCCAGTCTGGAACAGGGCTTTGAGCCCGCAGCCAAGACCTACCTCCAGAATTG
GCGCGAATGGCAGGCGCGGCTGCTGCCCCTGGACCGGCACGCGGCATCCGGCATCAACACCTATCGCACCTCGACCGCGG
TTCTGGCGACGCACCGCTCCATCGCCATGCCGGGCGCGGCGGTCGCAAGCCTGTCCATTCCCTGGGGCTTCAACAAGGGC
GACGACGACCTTGGCGGCTACCATCTGGTCTGGCCGCGCGATCTGGTCGAGACTGCGGGCGGCTTCCTCGCCGCCGGCGA
CGCCGCCTCGGCGCTGCAGATCCTTGAGTATCTGCGCTCCATCCAGCAGCCGGACGGCCATTGGCCGCAAAATGCCTGGC
TCGACGGGTCGGCCTATTGGCCCGGCATCCAGATGGACGAGTGCGCCTTTCCGCTGCTTCTGGCCGACGCCTTGCACCGC
GCCGGCCATCTGCCGCGCGCCAGGCTTGCCACCTTACTGCCGATGATCGAACGCGCCGCGACCTATGTCGTGCGCAATGG
ACCCGTCACCGGCGAAGACCGCTGGGAAGAGGATGCCGGCTACAGCCCGTTCACGCTTGCCGTCGAGATCGCCGCCCTGC
TTGCTGCGGCCGACCTGCTCGACGCCTGCGGCAAGAAGGACGAAGCAACCTATCTGCGCGAGACATCAGATGGCTGGAAC
GACCAGCTTGAGCGCTGGACCTATGCCACCGACACGGCGATCTGCAGGGCAGCCGGCGTCGAAGGTTATTATGTCCGTAT
CGCGCCACCCGACAGCGCCGAGGCCGGCTCGCCCAAGGACGGCTATGTCCCGATCAAGAACCGGCCGCCCGGCGACACCG
ACCGCCCGGCGCAGGAAATCGTCAGCCCCGACGCGCTGGCGCTGGTGCGCTTTGGCCTGAGGTCGGCCGACGACCCGCGC
ATCGTCGACACAGTCAAGGTCATCGACGCGCAGTTGCGCTGCGCGCTGCCGCAGGGCCCGATCTGGTATCGATACAATGG
CGACGGCTATGGCGAACACCAGGACGGCACGCCTTTCGACGGTACCGGCCAGGGCCGGCCCTGGCCGCTTCTGGCCGGCG
AGCGCGCCCATTATGAGCTGGCGGCGGGGCGCAAGGATGAGGCGGCCAACCTGCTCAAGGCGCTGGAGGATTCGGCCGAG
CCGGGCGGCCTGCTGCCGGAGCAGGTCTGGGATGGCGCCGACCTGCCCGAGCGCCAATTGCTGCATGGCCGCCCCTCGGG
CAGCGCCATGCCCCTGGTCTGGGCGCACTCCGAGCATATCAAGCTGCTGCGCTCGCTGCGCGACGGCGCCGTCTTCGACA
TGCCGCCGCAAGGCGTGAAGCGCTACATCGAGGCCAAGACCGTATCGCCATTCAGGACCTGGCGCTTCAACAACAAGATC
CGCGCGCTGCCCGAAGGCAAGACGCTGCGCATCGAATTGCTGGCCGCGGCCACGGTGCATTGGAGCACCGACAATTGGGC
AACCGCCCATGACAGCCAGACGGTGGAAAATGCCTTCGGCATCCATCTTGCCGATTTGCCCACGACCAGCCTCCCCGAGG
GAAGCGCGCTGATCTTCACTTTTTTCTGGCCCGGAACCGGCGATTGGGAGAATGTCGACTTCTCCGTCACATCAGGCGAG
CGGGATAGCGGCTAA

Upstream 100 bases:

>100_bases
TGTCAACTGCAATCCGCAAAGCCTGACGATCAACTTGCGGTGCGCCGATTGAAATGGCGTCGGTGGATGGGTAAGCCATG
CGCACAGGAGGCCAACAGAG

Downstream 100 bases:

>100_bases
CATCCTTCCTCGATATATCAAGACAATCTCTAGGAAACAGGACGAAACCATGCAGATCGGAATGATGGGACTGGGCCGGA
TGGGCGCCAACATGGTGCGG

Product: glucoamylase, (glucan 1,4-alpha-glucosidase)

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucohydrolase; Glucan 1,4-alpha-glucosidase [H]

Number of amino acids: Translated: 804; Mature: 803

Protein sequence:

>804_residues
MAVTPIVPPGAPGIPARWTSSAKSGVGTSLSPAGHIWFTISHGILEVYYPRLDSACTRDLGLIVTGPGGYFSEEKRDAAH
EIEPFEAGVPAYRLVNTAGDGAYRIEKRILCDPARPVLLQEITFTALKGAPDDYRVYALLAPHLVNAGMGNTAWVGDHKG
KPVLFASGRGSCLALASSLPWRDCSAGYVGFSDGWQQLNHTGILDQACQRAEDGNVALTGEIGFTSTTTKAVLALGFGAT
PDEAAENAFASLEQGFEPAAKTYLQNWREWQARLLPLDRHAASGINTYRTSTAVLATHRSIAMPGAAVASLSIPWGFNKG
DDDLGGYHLVWPRDLVETAGGFLAAGDAASALQILEYLRSIQQPDGHWPQNAWLDGSAYWPGIQMDECAFPLLLADALHR
AGHLPRARLATLLPMIERAATYVVRNGPVTGEDRWEEDAGYSPFTLAVEIAALLAAADLLDACGKKDEATYLRETSDGWN
DQLERWTYATDTAICRAAGVEGYYVRIAPPDSAEAGSPKDGYVPIKNRPPGDTDRPAQEIVSPDALALVRFGLRSADDPR
IVDTVKVIDAQLRCALPQGPIWYRYNGDGYGEHQDGTPFDGTGQGRPWPLLAGERAHYELAAGRKDEAANLLKALEDSAE
PGGLLPEQVWDGADLPERQLLHGRPSGSAMPLVWAHSEHIKLLRSLRDGAVFDMPPQGVKRYIEAKTVSPFRTWRFNNKI
RALPEGKTLRIELLAAATVHWSTDNWATAHDSQTVENAFGIHLADLPTTSLPEGSALIFTFFWPGTGDWENVDFSVTSGE
RDSG

Sequences:

>Translated_804_residues
MAVTPIVPPGAPGIPARWTSSAKSGVGTSLSPAGHIWFTISHGILEVYYPRLDSACTRDLGLIVTGPGGYFSEEKRDAAH
EIEPFEAGVPAYRLVNTAGDGAYRIEKRILCDPARPVLLQEITFTALKGAPDDYRVYALLAPHLVNAGMGNTAWVGDHKG
KPVLFASGRGSCLALASSLPWRDCSAGYVGFSDGWQQLNHTGILDQACQRAEDGNVALTGEIGFTSTTTKAVLALGFGAT
PDEAAENAFASLEQGFEPAAKTYLQNWREWQARLLPLDRHAASGINTYRTSTAVLATHRSIAMPGAAVASLSIPWGFNKG
DDDLGGYHLVWPRDLVETAGGFLAAGDAASALQILEYLRSIQQPDGHWPQNAWLDGSAYWPGIQMDECAFPLLLADALHR
AGHLPRARLATLLPMIERAATYVVRNGPVTGEDRWEEDAGYSPFTLAVEIAALLAAADLLDACGKKDEATYLRETSDGWN
DQLERWTYATDTAICRAAGVEGYYVRIAPPDSAEAGSPKDGYVPIKNRPPGDTDRPAQEIVSPDALALVRFGLRSADDPR
IVDTVKVIDAQLRCALPQGPIWYRYNGDGYGEHQDGTPFDGTGQGRPWPLLAGERAHYELAAGRKDEAANLLKALEDSAE
PGGLLPEQVWDGADLPERQLLHGRPSGSAMPLVWAHSEHIKLLRSLRDGAVFDMPPQGVKRYIEAKTVSPFRTWRFNNKI
RALPEGKTLRIELLAAATVHWSTDNWATAHDSQTVENAFGIHLADLPTTSLPEGSALIFTFFWPGTGDWENVDFSVTSGE
RDSG
>Mature_803_residues
AVTPIVPPGAPGIPARWTSSAKSGVGTSLSPAGHIWFTISHGILEVYYPRLDSACTRDLGLIVTGPGGYFSEEKRDAAHE
IEPFEAGVPAYRLVNTAGDGAYRIEKRILCDPARPVLLQEITFTALKGAPDDYRVYALLAPHLVNAGMGNTAWVGDHKGK
PVLFASGRGSCLALASSLPWRDCSAGYVGFSDGWQQLNHTGILDQACQRAEDGNVALTGEIGFTSTTTKAVLALGFGATP
DEAAENAFASLEQGFEPAAKTYLQNWREWQARLLPLDRHAASGINTYRTSTAVLATHRSIAMPGAAVASLSIPWGFNKGD
DDLGGYHLVWPRDLVETAGGFLAAGDAASALQILEYLRSIQQPDGHWPQNAWLDGSAYWPGIQMDECAFPLLLADALHRA
GHLPRARLATLLPMIERAATYVVRNGPVTGEDRWEEDAGYSPFTLAVEIAALLAAADLLDACGKKDEATYLRETSDGWND
QLERWTYATDTAICRAAGVEGYYVRIAPPDSAEAGSPKDGYVPIKNRPPGDTDRPAQEIVSPDALALVRFGLRSADDPRI
VDTVKVIDAQLRCALPQGPIWYRYNGDGYGEHQDGTPFDGTGQGRPWPLLAGERAHYELAAGRKDEAANLLKALEDSAEP
GGLLPEQVWDGADLPERQLLHGRPSGSAMPLVWAHSEHIKLLRSLRDGAVFDMPPQGVKRYIEAKTVSPFRTWRFNNKIR
ALPEGKTLRIELLAAATVHWSTDNWATAHDSQTVENAFGIHLADLPTTSLPEGSALIFTFFWPGTGDWENVDFSVTSGER
DSG

Specific function: CGA has typical kinetic properties for a glucoamylase, but this bacterial enzyme had higher isomaltose-hydrolyzing activity than other eukaryotic glucoamylases [H]

COG id: COG3387

COG function: function code G; Glucoamylase and related glycosyl hydrolases

Gene ontology:

Cell location: Cell membrane; Lipid-anchor [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 15 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR006425
- InterPro:   IPR015220
- InterPro:   IPR011013
- InterPro:   IPR014718
- InterPro:   IPR000165
- InterPro:   IPR011613 [H]

Pfam domain/function: PF09137 Glucodextran_N; PF00723 Glyco_hydro_15 [H]

EC number: =3.2.1.3 [H]

Molecular weight: Translated: 87033; Mature: 86902

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00820 GLUCOAMYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVTPIVPPGAPGIPARWTSSAKSGVGTSLSPAGHIWFTISHGILEVYYPRLDSACTRDL
CCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCEEEEEEECCEEEHHHHHHCHHHHCCC
GLIVTGPGGYFSEEKRDAAHEIEPFEAGVPAYRLVNTAGDGAYRIEKRILCDPARPVLLQ
CEEEECCCCCCCHHHHHHHHCCCCHHCCCCCEEEEECCCCCCEEEHHHEECCCCCCHHHH
EITFTALKGAPDDYRVYALLAPHLVNAGMGNTAWVGDHKGKPVLFASGRGSCLALASSLP
HHHHHHHCCCCCCEEEEEEECHHHHHCCCCCCEEECCCCCCEEEEECCCCCEEEEECCCC
WRDCSAGYVGFSDGWQQLNHTGILDQACQRAEDGNVALTGEIGFTSTTTKAVLALGFGAT
CCCCCCCCCCCCHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHEECCCCC
PDEAAENAFASLEQGFEPAAKTYLQNWREWQARLLPLDRHAASGINTYRTSTAVLATHRS
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHCCEEEEEECCH
IAMPGAAVASLSIPWGFNKGDDDLGGYHLVWPRDLVETAGGFLAAGDAASALQILEYLRS
HCCCCCEEEEEECCCCCCCCCCCCCCEEEECCHHHHHHCCCEEEECCHHHHHHHHHHHHH
IQQPDGHWPQNAWLDGSAYWPGIQMDECAFPLLLADALHRAGHLPRARLATLLPMIERAA
HCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TYVVRNGPVTGEDRWEEDAGYSPFTLAVEIAALLAAADLLDACGKKDEATYLRETSDGWN
HEEEECCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHH
DQLERWTYATDTAICRAAGVEGYYVRIAPPDSAEAGSPKDGYVPIKNRPPGDTDRPAQEI
HHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHH
VSPDALALVRFGLRSADDPRIVDTVKVIDAQLRCALPQGPIWYRYNGDGYGEHQDGTPFD
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCCCCCCCCC
GTGQGRPWPLLAGERAHYELAAGRKDEAANLLKALEDSAEPGGLLPEQVWDGADLPERQL
CCCCCCCCCEECCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCHHHH
LHGRPSGSAMPLVWAHSEHIKLLRSLRDGAVFDMPPQGVKRYIEAKTVSPFRTWRFNNKI
HCCCCCCCCCEEEEECHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCCCCHHEEEECCCE
RALPEGKTLRIELLAAATVHWSTDNWATAHDSQTVENAFGIHLADLPTTSLPEGSALIFT
EECCCCCEEEEEEEEEEEEEECCCCCCCCCCCHHHHHHCCEEEECCCCCCCCCCCEEEEE
FFWPGTGDWENVDFSVTSGERDSG
EECCCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
AVTPIVPPGAPGIPARWTSSAKSGVGTSLSPAGHIWFTISHGILEVYYPRLDSACTRDL
CCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCEEEEEEECCEEEHHHHHHCHHHHCCC
GLIVTGPGGYFSEEKRDAAHEIEPFEAGVPAYRLVNTAGDGAYRIEKRILCDPARPVLLQ
CEEEECCCCCCCHHHHHHHHCCCCHHCCCCCEEEEECCCCCCEEEHHHEECCCCCCHHHH
EITFTALKGAPDDYRVYALLAPHLVNAGMGNTAWVGDHKGKPVLFASGRGSCLALASSLP
HHHHHHHCCCCCCEEEEEEECHHHHHCCCCCCEEECCCCCCEEEEECCCCCEEEEECCCC
WRDCSAGYVGFSDGWQQLNHTGILDQACQRAEDGNVALTGEIGFTSTTTKAVLALGFGAT
CCCCCCCCCCCCHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHEECCCCC
PDEAAENAFASLEQGFEPAAKTYLQNWREWQARLLPLDRHAASGINTYRTSTAVLATHRS
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHCCEEEEEECCH
IAMPGAAVASLSIPWGFNKGDDDLGGYHLVWPRDLVETAGGFLAAGDAASALQILEYLRS
HCCCCCEEEEEECCCCCCCCCCCCCCEEEECCHHHHHHCCCEEEECCHHHHHHHHHHHHH
IQQPDGHWPQNAWLDGSAYWPGIQMDECAFPLLLADALHRAGHLPRARLATLLPMIERAA
HCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TYVVRNGPVTGEDRWEEDAGYSPFTLAVEIAALLAAADLLDACGKKDEATYLRETSDGWN
HEEEECCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHH
DQLERWTYATDTAICRAAGVEGYYVRIAPPDSAEAGSPKDGYVPIKNRPPGDTDRPAQEI
HHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHH
VSPDALALVRFGLRSADDPRIVDTVKVIDAQLRCALPQGPIWYRYNGDGYGEHQDGTPFD
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCCCCCCCCC
GTGQGRPWPLLAGERAHYELAAGRKDEAANLLKALEDSAEPGGLLPEQVWDGADLPERQL
CCCCCCCCCEECCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCHHHH
LHGRPSGSAMPLVWAHSEHIKLLRSLRDGAVFDMPPQGVKRYIEAKTVSPFRTWRFNNKI
HCCCCCCCCCEEEEECHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCCCCHHEEEECCCE
RALPEGKTLRIELLAAATVHWSTDNWATAHDSQTVENAFGIHLADLPTTSLPEGSALIFT
EECCCCCEEEEEEEEEEEEEECCCCCCCCCCCHHHHHHCCEEEECCCCCCCCCCCEEEEE
FFWPGTGDWENVDFSVTSGERDSG
EECCCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1633799 [H]