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The map label for this gene is nemA [H]

Identifier: 13472043

GI number: 13472043

Start: 1789066

End: 1790181

Strand: Reverse

Name: nemA [H]

Synonym: mll2211

Alternate gene names: 13472043

Gene position: 1790181-1789066 (Counterclockwise)

Preceding gene: 13472044

Following gene: 13472042

Centisome position: 25.44

GC content: 66.13

Gene sequence:

>1116_bases
ATGACTACCCTGTTTGATCCCTTGCGGGCCGGCGACCTGACGCTGGCGAACCGCATCGTCATGGCGCCGCTGACGCGCAA
TCGCTCGCCCAATGCGGTGCCCGGCGACCTCTCGGTGACCTATTACAGCCAGCGCGCCACGGCTGGTCTCATCGTGACCG
AGGCCACCGCCATCAGCCATCAGGGCCAGGGCTATGCCAACGTGCCCGGCCTCTATGGCGCGGATCAGCTGGCCGGCTGG
AGGCGTGTCACGGACGCCGTCCATAGAGGCGGCGGCAAGATTGTGGTGCAGCTCTGGCATGTCGGGCGCATCTCGCACAA
CACATTGCAGCCCGGCGGCGGCAAGCCGGTGGCGCCGTCGGCGATCAGGGCAAACTCCAAGACCTTCCTGGTCAAGCCGG
ATGGCAGCGGCGAGTTCGCCGAGACCTCCGAGCCGCGCGCGCTCGATGCGGCCGAACTTCCCGGCATCGTCGATGATTTC
CGCCGCGCCGCCAAGGCGGCGATCGAGGTGGCGGGTTTCGACGGCGTCGAGATCCATGGCGCCAACGGATATCTCATCGA
CCAGTTCCTGCGTTCAGGCACCAATCATCGTAACGACGCGTATGGCGGGTCGATCGAGAACCGGACGCGCTTCCTGTTCG
AGGTGGTCGACGCGGTCACGGGCGCAGTCGGTGCCGGCAGGACCGGGATCAGGCTGTCGCCGGTGACGCCCGCCAACGAC
GCTTCGGATAGCGATCCGCAGCCGCTGTTCAACCATGTGGTGGCGGGCCTCGGAGGTCGCGGGCTGGCCTATATCCACAT
CGTCGAAGGCGCGACCGGCGGCGCGCGCGACTTCAGCCAGGGCGACAGGCCGTTCGATTACGAGGAACTCAAGGCCACCT
ATCGCAAGGCCGGCGGCGCTGGCGCCTGGCTGGTGAACAATGGCTACGACAAGGAACTGGCCGAAAAAGCGGTCGGTGAC
GGCTATGCCGAGCTTGTCGCGTTCGGCAAGCTGTTCATCGCCAACCCCGACCTCGTCAGCCGGCTGAAGCGCAATGCCGA
ACTGAATGCCCCTGACAAGGCGACGTTCTATGGCGGCGATGCCAGGGGCTATACGGATTACCCGACGGCGGCCTGA

Upstream 100 bases:

>100_bases
TACAAGCGCAACGAAGAAACCATCGCCGCCTTTCAGGCCGCCTTGTCCGATCTCTGACGATCCTCCCCCGATCCCCCAGA
ATGCATGAAAGGCATTTCTC

Downstream 100 bases:

>100_bases
GCCGCCCATCCCAGACGGTGTGCTCCTTTGCTTGCACACAAGGCAGCCGGCTCCTCAATGAGGGGCCGGTTTGGCTGCTA
TGCTGGTCCGCGGCCAGATT

Product: morphinone reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MTTLFDPLRAGDLTLANRIVMAPLTRNRSPNAVPGDLSVTYYSQRATAGLIVTEATAISHQGQGYANVPGLYGADQLAGW
RRVTDAVHRGGGKIVVQLWHVGRISHNTLQPGGGKPVAPSAIRANSKTFLVKPDGSGEFAETSEPRALDAAELPGIVDDF
RRAAKAAIEVAGFDGVEIHGANGYLIDQFLRSGTNHRNDAYGGSIENRTRFLFEVVDAVTGAVGAGRTGIRLSPVTPAND
ASDSDPQPLFNHVVAGLGGRGLAYIHIVEGATGGARDFSQGDRPFDYEELKATYRKAGGAGAWLVNNGYDKELAEKAVGD
GYAELVAFGKLFIANPDLVSRLKRNAELNAPDKATFYGGDARGYTDYPTAA

Sequences:

>Translated_371_residues
MTTLFDPLRAGDLTLANRIVMAPLTRNRSPNAVPGDLSVTYYSQRATAGLIVTEATAISHQGQGYANVPGLYGADQLAGW
RRVTDAVHRGGGKIVVQLWHVGRISHNTLQPGGGKPVAPSAIRANSKTFLVKPDGSGEFAETSEPRALDAAELPGIVDDF
RRAAKAAIEVAGFDGVEIHGANGYLIDQFLRSGTNHRNDAYGGSIENRTRFLFEVVDAVTGAVGAGRTGIRLSPVTPAND
ASDSDPQPLFNHVVAGLGGRGLAYIHIVEGATGGARDFSQGDRPFDYEELKATYRKAGGAGAWLVNNGYDKELAEKAVGD
GYAELVAFGKLFIANPDLVSRLKRNAELNAPDKATFYGGDARGYTDYPTAA
>Mature_370_residues
TTLFDPLRAGDLTLANRIVMAPLTRNRSPNAVPGDLSVTYYSQRATAGLIVTEATAISHQGQGYANVPGLYGADQLAGWR
RVTDAVHRGGGKIVVQLWHVGRISHNTLQPGGGKPVAPSAIRANSKTFLVKPDGSGEFAETSEPRALDAAELPGIVDDFR
RAAKAAIEVAGFDGVEIHGANGYLIDQFLRSGTNHRNDAYGGSIENRTRFLFEVVDAVTGAVGAGRTGIRLSPVTPANDA
SDSDPQPLFNHVVAGLGGRGLAYIHIVEGATGGARDFSQGDRPFDYEELKATYRKAGGAGAWLVNNGYDKELAEKAVGDG
YAELVAFGKLFIANPDLVSRLKRNAELNAPDKATFYGGDARGYTDYPTAA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=370, Percent_Identity=49.4594594594595, Blast_Score=332, Evalue=2e-92,
Organism=Escherichia coli, GI1789463, Length=224, Percent_Identity=37.9464285714286, Blast_Score=121, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17559802, Length=281, Percent_Identity=28.8256227758007, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17559804, Length=141, Percent_Identity=38.2978723404255, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17540738, Length=134, Percent_Identity=39.5522388059701, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI72001454, Length=158, Percent_Identity=34.8101265822785, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17564188, Length=141, Percent_Identity=34.7517730496454, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17565138, Length=147, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17566914, Length=223, Percent_Identity=29.5964125560538, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6325086, Length=387, Percent_Identity=37.2093023255814, Blast_Score=216, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6321973, Length=389, Percent_Identity=36.5038560411311, Blast_Score=210, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39270; Mature: 39139

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLFDPLRAGDLTLANRIVMAPLTRNRSPNAVPGDLSVTYYSQRATAGLIVTEATAISH
CCCCCCCCCCCCEEHHCEEEEEEHHCCCCCCCCCCCEEEEEECCCCCCEEEEEEEHHHHC
QGQGYANVPGLYGADQLAGWRRVTDAVHRGGGKIVVQLWHVGRISHNTLQPGGGKPVAPS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCH
AIRANSKTFLVKPDGSGEFAETSEPRALDAAELPGIVDDFRRAAKAAIEVAGFDGVEIHG
HEECCCCEEEECCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHEEEECCCCEEEEC
ANGYLIDQFLRSGTNHRNDAYGGSIENRTRFLFEVVDAVTGAVGAGRTGIRLSPVTPAND
CCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCC
ASDSDPQPLFNHVVAGLGGRGLAYIHIVEGATGGARDFSQGDRPFDYEELKATYRKAGGA
CCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
GAWLVNNGYDKELAEKAVGDGYAELVAFGKLFIANPDLVSRLKRNAELNAPDKATFYGGD
EEEEECCCCCHHHHHHHHCCCHHHHHHHHHHEECCHHHHHHHHHCCCCCCCCCCEEECCC
ARGYTDYPTAA
CCCCCCCCCCC
>Mature Secondary Structure 
TTLFDPLRAGDLTLANRIVMAPLTRNRSPNAVPGDLSVTYYSQRATAGLIVTEATAISH
CCCCCCCCCCCEEHHCEEEEEEHHCCCCCCCCCCCEEEEEECCCCCCEEEEEEEHHHHC
QGQGYANVPGLYGADQLAGWRRVTDAVHRGGGKIVVQLWHVGRISHNTLQPGGGKPVAPS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCH
AIRANSKTFLVKPDGSGEFAETSEPRALDAAELPGIVDDFRRAAKAAIEVAGFDGVEIHG
HEECCCCEEEECCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHEEEECCCCEEEEC
ANGYLIDQFLRSGTNHRNDAYGGSIENRTRFLFEVVDAVTGAVGAGRTGIRLSPVTPAND
CCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCC
ASDSDPQPLFNHVVAGLGGRGLAYIHIVEGATGGARDFSQGDRPFDYEELKATYRKAGGA
CCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
GAWLVNNGYDKELAEKAVGDGYAELVAFGKLFIANPDLVSRLKRNAELNAPDKATFYGGD
EEEEECCCCCHHHHHHHHCCCHHHHHHHHHHEECCHHHHHHHHHCCCCCCCCCCEEECCC
ARGYTDYPTAA
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]