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The map label for this gene is ampR [H]

Identifier: 13471463

GI number: 13471463

Start: 1203054

End: 1203941

Strand: Direct

Name: ampR [H]

Synonym: mlr1441

Alternate gene names: 13471463

Gene position: 1203054-1203941 (Clockwise)

Preceding gene: 13471460

Following gene: 13471464

Centisome position: 17.1

GC content: 68.36

Gene sequence:

>888_bases
ATGCCTCGAAAACCCATTCCCGCCCTGCCTCCCCTCGACTGGCTGCGCAGTTTCGAGGCTGCGGCACGGCTGTCGAATTT
CACGGCGGCCGCGGCCGAACTCGGCCTGACCCAGGCGGCCGTCAGCCAGCATATCCGGCTGCTCGAGGAGCGGTTGAAGA
CGCGCCTGTTCTCGCGGCTGGCGCGCGGCGTCGCACTGTCGCCGGAGGGTGCCGCCTATCTGCCGCACATCCAGTCGGCT
TTCGCCACCATCTCCAGCAGCACGACGGAACTGTTCGAGCCGCGCGCTGTGCAGACCGTGTCGATCCGGGTGCCGATCTC
TTTCGCCTTGCTTATCCTGGTCCCGGCGCTGCCCGATCTCGCCGAGGCGCTGCCGCGCATCCAGCTCGATCTGGTGACGA
TCCATCGGCCGACCGACTATGATTTGCCGGGATCCGCGCTCGACATCCGTTTCGGCAACGGCTCCTTTCCCGGACGCGAG
GCCGGCAGGCTGACTGCCGAGCGGCTTGTGCCGGTGGCTAGTCCAGCGCTGGCCGGTGCCGCCGACTGGACGGCCCTGCC
GCTGCTTCTGGTCGCCGGTGCGCGCGAAATGTGGGCGGAATGGTTCGCGGCAGCCGGACTGGCCGGCCACCCCCAACGAT
CGCACCGCTTCGACAGTTTCGTCGCGGCGATGGAAGCGGCGAGCGCCGGCGCCGGCGTGCTTCTGGGGTCGCGGCCGCTT
GTTGACGCGGCGCTGAGGAACAGGACCCTGGTGCCGCTTTCCGATTTCGAGCTCTCCAGCAGCTCAGGCCATTTTCTGAC
CAGCGCATCGACCGCGCGCCTGACCAGCGCCGAACAGGATTTCCGCCAGTGGCTGCTGGGGCACCTGTCCAGAAAGGCCC
CGGCCTGA

Upstream 100 bases:

>100_bases
TACGCCCCGCGGCCCGGTCCGCAGGGTTTCGATTGCATGTTGGCGACAAGAAAGGCATAACTCAAATTATCATTTTTTGG
CCAAAGACCACAAATTTCTA

Downstream 100 bases:

>100_bases
TCGTCACGGTCTGGCGCGATGTGGCGCCGACACGCTTCCAGCAGATCAGCGTGCCGATCAGCCCGCCATGCCGGCGTCAA
TCGAGATGGCGCACGCGGCG

Product: transcriptional activator protein ampR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MPRKPIPALPPLDWLRSFEAAARLSNFTAAAAELGLTQAAVSQHIRLLEERLKTRLFSRLARGVALSPEGAAYLPHIQSA
FATISSSTTELFEPRAVQTVSIRVPISFALLILVPALPDLAEALPRIQLDLVTIHRPTDYDLPGSALDIRFGNGSFPGRE
AGRLTAERLVPVASPALAGAADWTALPLLLVAGAREMWAEWFAAAGLAGHPQRSHRFDSFVAAMEAASAGAGVLLGSRPL
VDAALRNRTLVPLSDFELSSSSGHFLTSASTARLTSAEQDFRQWLLGHLSRKAPA

Sequences:

>Translated_295_residues
MPRKPIPALPPLDWLRSFEAAARLSNFTAAAAELGLTQAAVSQHIRLLEERLKTRLFSRLARGVALSPEGAAYLPHIQSA
FATISSSTTELFEPRAVQTVSIRVPISFALLILVPALPDLAEALPRIQLDLVTIHRPTDYDLPGSALDIRFGNGSFPGRE
AGRLTAERLVPVASPALAGAADWTALPLLLVAGAREMWAEWFAAAGLAGHPQRSHRFDSFVAAMEAASAGAGVLLGSRPL
VDAALRNRTLVPLSDFELSSSSGHFLTSASTARLTSAEQDFRQWLLGHLSRKAPA
>Mature_294_residues
PRKPIPALPPLDWLRSFEAAARLSNFTAAAAELGLTQAAVSQHIRLLEERLKTRLFSRLARGVALSPEGAAYLPHIQSAF
ATISSSTTELFEPRAVQTVSIRVPISFALLILVPALPDLAEALPRIQLDLVTIHRPTDYDLPGSALDIRFGNGSFPGREA
GRLTAERLVPVASPALAGAADWTALPLLLVAGAREMWAEWFAAAGLAGHPQRSHRFDSFVAAMEAASAGAGVLLGSRPLV
DAALRNRTLVPLSDFELSSSSGHFLTSASTARLTSAEQDFRQWLLGHLSRKAPA

Specific function: This protein is a positive regulator of gene expression of cephalosporinase (AmpC) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=286, Percent_Identity=32.5174825174825, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=32.8719723183391, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788706, Length=268, Percent_Identity=29.1044776119403, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI87081978, Length=192, Percent_Identity=34.375, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787128, Length=119, Percent_Identity=33.6134453781513, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693193, Length=167, Percent_Identity=31.1377245508982, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31576; Mature: 31444

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRKPIPALPPLDWLRSFEAAARLSNFTAAAAELGLTQAAVSQHIRLLEERLKTRLFSRL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARGVALSPEGAAYLPHIQSAFATISSSTTELFEPRAVQTVSIRVPISFALLILVPALPDL
HHCCEECCCCCCHHHHHHHHHHHHHCCHHHHHCCCCEEEEEEECHHHHHHHHHHHCCHHH
AEALPRIQLDLVTIHRPTDYDLPGSALDIRFGNGSFPGREAGRLTAERLVPVASPALAGA
HHHCCCEEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHCCC
ADWTALPLLLVAGAREMWAEWFAAAGLAGHPQRSHRFDSFVAAMEAASAGAGVLLGSRPL
CHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCH
VDAALRNRTLVPLSDFELSSSSGHFLTSASTARLTSAEQDFRQWLLGHLSRKAPA
HHHHHCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PRKPIPALPPLDWLRSFEAAARLSNFTAAAAELGLTQAAVSQHIRLLEERLKTRLFSRL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARGVALSPEGAAYLPHIQSAFATISSSTTELFEPRAVQTVSIRVPISFALLILVPALPDL
HHCCEECCCCCCHHHHHHHHHHHHHCCHHHHHCCCCEEEEEEECHHHHHHHHHHHCCHHH
AEALPRIQLDLVTIHRPTDYDLPGSALDIRFGNGSFPGREAGRLTAERLVPVASPALAGA
HHHCCCEEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHCCC
ADWTALPLLLVAGAREMWAEWFAAAGLAGHPQRSHRFDSFVAAMEAASAGAGVLLGSRPL
CHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCH
VDAALRNRTLVPLSDFELSSSSGHFLTSASTARLTSAEQDFRQWLLGHLSRKAPA
HHHHHCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3030737 [H]