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The map label for this gene is cobS

Identifier: 13471422

GI number: 13471422

Start: 1163123

End: 1163902

Strand: Direct

Name: cobS

Synonym: mlr1388

Alternate gene names: 13471422

Gene position: 1163123-1163902 (Clockwise)

Preceding gene: 13471421

Following gene: 13471423

Centisome position: 16.53

GC content: 67.56

Gene sequence:

>780_bases
ATGAAGCTGCCGAATTTGACCCTTTCGCCCCGGCAAATCCTCGACGATGTCGCGCTCTGCCTGGTCTTCTTCACCCGCCT
GCCTTTGCCCGACCTCGACTTTCGCGGCCGCGGCCTTGCCGCCGCGATCTGGGCCGCACCGGTCGCCGGCCTCCTCGTCG
GGCTGATCGGCGCCATCGTCTTTGCCACGGCGGAACGGTTCGGCCTTGCCATGGGGCCGGCGGCAGCACTTGCCCTTGTT
GCAACCGTGATCGCCACCGGATGCCTGCACGAGGATGGACTGTCCGACGTTGCGGACGGCTTTGGCGGTGGCAAGTCGCG
CGGCCGCAAACTCGACATCATGCGCGACAGCCGAATCGGCGCCTATGGTGCCATGGCGCTGGCGCTGTCGCTGCTGATCC
GCTGGAACGTGCTTTCGGAACTGGTGGATCCCACTCAGGCTCTCTTTGCCCTTGTTGCCGCGCATGCGGCCTCGCGCGGC
GTGCTGGGCGCCTTCATGCACCTCTTGCCGCCGGCGCGCTCCGATGGCCTCTCGGCCGGCGCCGGCGCCGTCTCGCTTGA
AACCGCCATCGCCGGCGCCGTGCTCGGCGCGATCCCGCTGCTCCTGCTCGGAGCCGGCGGCGCCATCGCCGCGCTCATCC
TGCTTGGCCTGCTCTTTGCCGCCTTCCATGCCCTTTGCCTCAACCAGATAGGTGGCCAGACCGGCGACACGATCGGTGCC
CTGCAGCAGGTCAGCGAAATCGCGGTGCTTCTCGTCGCTTCCGTAGCGCTTTCCTCTTGA

Upstream 100 bases:

>100_bases
CCGACCTCGCCGACGGCCTGGGCAATCCGATCGGGGCTTCCGGCTACCGGCGTGGTCATGTCAGCGGTACCTTCTTCCAT
GCGATAGCGAGGGCCAGTGC

Downstream 100 bases:

>100_bases
CCTGATTCCCTTTCGGAGACCTTGCCCCCATGCCCTTCAAATCCCTCGATGAATTGCGCGCCGCTTGCCTCGATCTGCCC
GCCGGCAGCGATACCGCCGC

Product: cobalamin (5'-phosphate) synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MKLPNLTLSPRQILDDVALCLVFFTRLPLPDLDFRGRGLAAAIWAAPVAGLLVGLIGAIVFATAERFGLAMGPAAALALV
ATVIATGCLHEDGLSDVADGFGGGKSRGRKLDIMRDSRIGAYGAMALALSLLIRWNVLSELVDPTQALFALVAAHAASRG
VLGAFMHLLPPARSDGLSAGAGAVSLETAIAGAVLGAIPLLLLGAGGAIAALILLGLLFAAFHALCLNQIGGQTGDTIGA
LQQVSEIAVLLVASVALSS

Sequences:

>Translated_259_residues
MKLPNLTLSPRQILDDVALCLVFFTRLPLPDLDFRGRGLAAAIWAAPVAGLLVGLIGAIVFATAERFGLAMGPAAALALV
ATVIATGCLHEDGLSDVADGFGGGKSRGRKLDIMRDSRIGAYGAMALALSLLIRWNVLSELVDPTQALFALVAAHAASRG
VLGAFMHLLPPARSDGLSAGAGAVSLETAIAGAVLGAIPLLLLGAGGAIAALILLGLLFAAFHALCLNQIGGQTGDTIGA
LQQVSEIAVLLVASVALSS
>Mature_259_residues
MKLPNLTLSPRQILDDVALCLVFFTRLPLPDLDFRGRGLAAAIWAAPVAGLLVGLIGAIVFATAERFGLAMGPAAALALV
ATVIATGCLHEDGLSDVADGFGGGKSRGRKLDIMRDSRIGAYGAMALALSLLIRWNVLSELVDPTQALFALVAAHAASRG
VLGAFMHLLPPARSDGLSAGAGAVSLETAIAGAVLGAIPLLLLGAGGAIAALILLGLLFAAFHALCLNQIGGQTGDTIGA
LQQVSEIAVLLVASVALSS

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=243, Percent_Identity=37.037037037037, Blast_Score=100, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_RHILO (Q98KP0)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102988.1
- GeneID:   1225651
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr1388
- NMPDR:   fig|266835.1.peg.1093
- HOGENOM:   HBG729392
- OMA:   ELTECAV
- ProtClustDB:   CLSK339417
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 26130; Mature: 26130

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xdbb6d94)-; HASH(0xde02c40)-; HASH(0xe0fb6dc)-; HASH(0xd57b854)-; HASH(0xe156484)-; HASH(0xdc1a814)-; HASH(0xc81960c)-;

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11214968