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The map label for this gene is cobB

Identifier: 13471421

GI number: 13471421

Start: 1161807

End: 1163126

Strand: Direct

Name: cobB

Synonym: mlr1387

Alternate gene names: 13471421

Gene position: 1161807-1163126 (Clockwise)

Preceding gene: 13471420

Following gene: 13471422

Centisome position: 16.51

GC content: 67.73

Gene sequence:

>1320_bases
ATGACGGCCCGCGCCATCATCATCGGCGCGCCGCGCTCCGGCTCGGGCAAGACCAGCGTCACCATCGGCATCTTGCGCGC
GCTCACCCGGCGCGGCCTGAAAGTGCGCGGCGCCAAATCCGGCCCCGACTATATCGATCCCGGTTTCCACACCGCCGCCA
CCGGCCTGTCCGGCGTCAACCTCGACAGCTGGGCGATGCCGCCATCGCTGCTCAATGCGCTGGCCGCGCAACAGGCCGAC
GACACCGATTTCGTCATCCTCGAAAGCGCCATGGGCCTGTTCGACGGCATTCCGGCCGCGCCGGGGCGAACCGGTTCGGC
CGCAGATCTCGCCCGGCTCTACGGCCTGCCGGTGCTGCTGGTGCTCGACGTGTCAGGCCAGTCGACCACGGCCGCCGCCG
TCGCCAAGGGCTTTGCCACCTACGATCCGGATGTGCGCATGGCCGGCGTTGTGCTCAACCGGCTGGGCAGCGAACGCCAT
CGCAAGCTGTCCGGCGACGCCATCGAGGCGATCGGCCTGCCCGTCGTCGGCGCCATTCTGCGCGATCCCACGCTCAATCT
GCCCGAACGGCATCTCGGCCTCGTCCAGGCTGGCGAGTATGATGATCTGATGGCGCATCTCGACCGGCTGGCCGACATGG
CGGAGAAGTCGCTCGACCTCGATGCCGTGATGAGGCTGGCGACGCCCCTCGCTCCGGCGGCTGGCGGATTCGCCGACGCG
CTGCAGCCGCCCGGCCAACGCATCGCGCTCGCCGAGGATGGCGCCTTTACTTTCCTTTACCCGCACGTCGCCGCCTATTG
GCGCAAGGCCGGCGCGGAGATCGTTCCGTTCTCGCCGCTCGCCGACGAGGCGCCCGACGACAGCTGCGATGTCTGCTGGC
TGCCCGGCGGCTATCCCGAGCTCCATGCCGGCAAGCTTGCAGCCGCCGAGACCTTCAAAGCCGGCATGGCAAGATTTGCG
GCGACGAAGCCGATCCATGGCGAGTGCGGCGGCTTCATGGTGCTTGGGGAAGCGCTGGAGGATGCATCAGGCGAAACGCA
TCGCATGCTCGGCCTGCTTGGCCATGCCACCAGTTTTGCAAAGCGAAAGATGAATCTCGGCTATCGCGAGGCGCGGTTGC
GCGCCGATTGCCCGCTGGGCGCACAGGGCGCGCTGATCCGCGGCCATGAATTCCACTATGCGCAGATGACGGCCACCGGG
AATGACGAGCCGCTGGCCGACCTCGCCGACGGCCTGGGCAATCCGATCGGGGCTTCCGGCTACCGGCGTGGTCATGTCAG
CGGTACCTTCTTCCATGCGATAGCGAGGGCCAGTGCATGA

Upstream 100 bases:

>100_bases
CCACAATCGCCGAGGAAGCGGCCGCCGCGGGGCTGACCTCGCCGGCGCTGATCGTCGTCGGCGGCATCGTCGCCATGCGG
GCGGCATTGACGAGGCAGGC

Downstream 100 bases:

>100_bases
AGCTGCCGAATTTGACCCTTTCGCCCCGGCAAATCCTCGACGATGTCGCGCTCTGCCTGGTCTTCTTCACCCGCCTGCCT
TTGCCCGACCTCGACTTTCG

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MTARAIIIGAPRSGSGKTSVTIGILRALTRRGLKVRGAKSGPDYIDPGFHTAATGLSGVNLDSWAMPPSLLNALAAQQAD
DTDFVILESAMGLFDGIPAAPGRTGSAADLARLYGLPVLLVLDVSGQSTTAAAVAKGFATYDPDVRMAGVVLNRLGSERH
RKLSGDAIEAIGLPVVGAILRDPTLNLPERHLGLVQAGEYDDLMAHLDRLADMAEKSLDLDAVMRLATPLAPAAGGFADA
LQPPGQRIALAEDGAFTFLYPHVAAYWRKAGAEIVPFSPLADEAPDDSCDVCWLPGGYPELHAGKLAAAETFKAGMARFA
ATKPIHGECGGFMVLGEALEDASGETHRMLGLLGHATSFAKRKMNLGYREARLRADCPLGAQGALIRGHEFHYAQMTATG
NDEPLADLADGLGNPIGASGYRRGHVSGTFFHAIARASA

Sequences:

>Translated_439_residues
MTARAIIIGAPRSGSGKTSVTIGILRALTRRGLKVRGAKSGPDYIDPGFHTAATGLSGVNLDSWAMPPSLLNALAAQQAD
DTDFVILESAMGLFDGIPAAPGRTGSAADLARLYGLPVLLVLDVSGQSTTAAAVAKGFATYDPDVRMAGVVLNRLGSERH
RKLSGDAIEAIGLPVVGAILRDPTLNLPERHLGLVQAGEYDDLMAHLDRLADMAEKSLDLDAVMRLATPLAPAAGGFADA
LQPPGQRIALAEDGAFTFLYPHVAAYWRKAGAEIVPFSPLADEAPDDSCDVCWLPGGYPELHAGKLAAAETFKAGMARFA
ATKPIHGECGGFMVLGEALEDASGETHRMLGLLGHATSFAKRKMNLGYREARLRADCPLGAQGALIRGHEFHYAQMTATG
NDEPLADLADGLGNPIGASGYRRGHVSGTFFHAIARASA
>Mature_438_residues
TARAIIIGAPRSGSGKTSVTIGILRALTRRGLKVRGAKSGPDYIDPGFHTAATGLSGVNLDSWAMPPSLLNALAAQQADD
TDFVILESAMGLFDGIPAAPGRTGSAADLARLYGLPVLLVLDVSGQSTTAAAVAKGFATYDPDVRMAGVVLNRLGSERHR
KLSGDAIEAIGLPVVGAILRDPTLNLPERHLGLVQAGEYDDLMAHLDRLADMAEKSLDLDAVMRLATPLAPAAGGFADAL
QPPGQRIALAEDGAFTFLYPHVAAYWRKAGAEIVPFSPLADEAPDDSCDVCWLPGGYPELHAGKLAAAETFKAGMARFAA
TKPIHGECGGFMVLGEALEDASGETHRMLGLLGHATSFAKRKMNLGYREARLRADCPLGAQGALIRGHEFHYAQMTATGN
DEPLADLADGLGNPIGASGYRRGHVSGTFFHAIARASA

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBB_RHILO (Q98KP1)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102987.1
- ProteinModelPortal:   Q98KP1
- GeneID:   1225650
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr1387
- NMPDR:   fig|266835.1.peg.1092
- HOGENOM:   HBG634910
- OMA:   VFKCGPD
- ProtClustDB:   PRK01077
- HAMAP:   MF_00027
- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00379

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.9

Molecular weight: Translated: 45880; Mature: 45749

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS51274 GATASE_COBBQ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: ACT_SITE 329-329 ACT_SITE 432-432

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARAIIIGAPRSGSGKTSVTIGILRALTRRGLKVRGAKSGPDYIDPGFHTAATGLSGVN
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHCCCCCCC
LDSWAMPPSLLNALAAQQADDTDFVILESAMGLFDGIPAAPGRTGSAADLARLYGLPVLL
CCCCCCCHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCEEE
VLDVSGQSTTAAAVAKGFATYDPDVRMAGVVLNRLGSERHRKLSGDAIEAIGLPVVGAIL
EEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCHHHHHCHHHHHHHH
RDPTLNLPERHLGLVQAGEYDDLMAHLDRLADMAEKSLDLDAVMRLATPLAPAAGGFADA
CCCCCCCCHHHHCEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHH
LQPPGQRIALAEDGAFTFLYPHVAAYWRKAGAEIVPFSPLADEAPDDSCDVCWLPGGYPE
CCCCCCEEEEECCCCEEEEHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCEEECCCCCCC
LHAGKLAAAETFKAGMARFAATKPIHGECGGFMVLGEALEDASGETHRMLGLLGHATSFA
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHH
KRKMNLGYREARLRADCPLGAQGALIRGHEFHYAQMTATGNDEPLADLADGLGNPIGASG
HHHHCCCHHHHHHCCCCCCCCCCCEEECCEEEEEEEEECCCCCCHHHHHHHCCCCCCCCC
YRRGHVSGTFFHAIARASA
CCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TARAIIIGAPRSGSGKTSVTIGILRALTRRGLKVRGAKSGPDYIDPGFHTAATGLSGVN
CCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHCCCCCCC
LDSWAMPPSLLNALAAQQADDTDFVILESAMGLFDGIPAAPGRTGSAADLARLYGLPVLL
CCCCCCCHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCEEE
VLDVSGQSTTAAAVAKGFATYDPDVRMAGVVLNRLGSERHRKLSGDAIEAIGLPVVGAIL
EEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCHHHHHCHHHHHHHH
RDPTLNLPERHLGLVQAGEYDDLMAHLDRLADMAEKSLDLDAVMRLATPLAPAAGGFADA
CCCCCCCCHHHHCEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHH
LQPPGQRIALAEDGAFTFLYPHVAAYWRKAGAEIVPFSPLADEAPDDSCDVCWLPGGYPE
CCCCCCEEEEECCCCEEEEHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCEEECCCCCCC
LHAGKLAAAETFKAGMARFAATKPIHGECGGFMVLGEALEDASGETHRMLGLLGHATSFA
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHH
KRKMNLGYREARLRADCPLGAQGALIRGHEFHYAQMTATGNDEPLADLADGLGNPIGASG
HHHHCCCHHHHHHCCCCCCCCCCCEEECCEEEEEEEEECCCCCCHHHHHHHCCCCCCCCC
YRRGHVSGTFFHAIARASA
CCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968