Click here to switch to the map view.

The map label for this gene is purR [H]

Identifier: 13471120

GI number: 13471120

Start: 830776

End: 831771

Strand: Direct

Name: purR [H]

Synonym: mlr1005

Alternate gene names: 13471120

Gene position: 830776-831771 (Clockwise)

Preceding gene: 13471109

Following gene: 13471121

Centisome position: 11.81

GC content: 58.94

Gene sequence:

>996_bases
ATGAGAAGGCGGGCAACGGCGAAGAACGTGGCAGATGCGGCCGGCGTCTCAAAATGGACAGTTATCCGTGCGTTCACGCC
GGGCGCATCGATTACCGAGGAGAGCAAACGTAGGGTTCTGCAGGCTGCAGCGGCGCTTAACTACTCGCCGAACCTCCTCG
CCCGCAGCTTGGCCACCAATCTCACGCATCAGGTGGCGGTCTTCGTCGACGACTTTGCCAACCCTCAGAAGTTGCCCTTC
CTGGAGACTTTGACCGATCGGCTGCAGGCTGAAGGGCTGGTCGCCATGCTGATCAACATCAACAATCACTTCGATCATGT
GCGTGCCCTGCTCAACGCGGATCAGCGCCAGGTCGATGCCATCATCATGTTCGGCACTGCTTTTCGCAGCGAAACGTTGA
ATGACAGGCGGCTGGGCCGGAGCATGCCGCCGATGTTCGTGCTGGCAAGGGACAGCCAGATCTATGGCGTGCCTGCCGTT
GTCTGTGACGCCGAGCTGGCATTGAAGGAGATCGTCGATCATCTTTATGAAAAGGGATACCGGCGTCCGGGGTTCATGGC
TGGGGCGCCGGCCCTCTCCACGGCGCTCAGGCGCCGGCATCATTTCATCGATTTCTGGAAACAGAAGGGCGTAGACGAGA
CCGCGGTGATATCGGCGGAAAGGTATAGCGCGCAGGCCGGAGCAGAATCCGTTCGCCACTATCTTCAGAATACGGATGCC
AAGGATAGGGTCGACGTCCTCATGTGCGAGAACGACATCCTGGCCATCGGCGCGCTTGACGAAATCCGCGGGCAGTTTGG
CTTGCGTGTGCCGCAGGACATGGCCGTCATCGGCTTCGACAATTACGAGCTGGGCGGAGCATCGGTCTATGGCCTTTCCA
CATACGAGCAGCCGAAGGACGAAATGGTTAAGGTTGTCATCGAGATGATAACCGGGCGGCTGCCCCCTGAGACTGTCACA
CTGCCGGGAAAGCTCATCGTCAGGAATTCTGCCTGA

Upstream 100 bases:

>100_bases
GGGTGCAAACGCGTGCATCATAGCTTTTGATGCGCTAAAAGCAAGCGTTCATTTCACGGAATCAGAAAATGGAATGGACG
TTTCATTTTGTGGAGGCTTC

Downstream 100 bases:

>100_bases
GCCACTTCTCAGAGCCAATCGGGGACACGGCGAACGTTGTCCTGCCGAGGCCTCTCAGTTCCAATCGAACATTATGCAGG
GAATGCAATGACCCAGATTG

Product: transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MRRRATAKNVADAAGVSKWTVIRAFTPGASITEESKRRVLQAAAALNYSPNLLARSLATNLTHQVAVFVDDFANPQKLPF
LETLTDRLQAEGLVAMLININNHFDHVRALLNADQRQVDAIIMFGTAFRSETLNDRRLGRSMPPMFVLARDSQIYGVPAV
VCDAELALKEIVDHLYEKGYRRPGFMAGAPALSTALRRRHHFIDFWKQKGVDETAVISAERYSAQAGAESVRHYLQNTDA
KDRVDVLMCENDILAIGALDEIRGQFGLRVPQDMAVIGFDNYELGGASVYGLSTYEQPKDEMVKVVIEMITGRLPPETVT
LPGKLIVRNSA

Sequences:

>Translated_331_residues
MRRRATAKNVADAAGVSKWTVIRAFTPGASITEESKRRVLQAAAALNYSPNLLARSLATNLTHQVAVFVDDFANPQKLPF
LETLTDRLQAEGLVAMLININNHFDHVRALLNADQRQVDAIIMFGTAFRSETLNDRRLGRSMPPMFVLARDSQIYGVPAV
VCDAELALKEIVDHLYEKGYRRPGFMAGAPALSTALRRRHHFIDFWKQKGVDETAVISAERYSAQAGAESVRHYLQNTDA
KDRVDVLMCENDILAIGALDEIRGQFGLRVPQDMAVIGFDNYELGGASVYGLSTYEQPKDEMVKVVIEMITGRLPPETVT
LPGKLIVRNSA
>Mature_331_residues
MRRRATAKNVADAAGVSKWTVIRAFTPGASITEESKRRVLQAAAALNYSPNLLARSLATNLTHQVAVFVDDFANPQKLPF
LETLTDRLQAEGLVAMLININNHFDHVRALLNADQRQVDAIIMFGTAFRSETLNDRRLGRSMPPMFVLARDSQIYGVPAV
VCDAELALKEIVDHLYEKGYRRPGFMAGAPALSTALRRRHHFIDFWKQKGVDETAVISAERYSAQAGAESVRHYLQNTDA
KDRVDVLMCENDILAIGALDEIRGQFGLRVPQDMAVIGFDNYELGGASVYGLSTYEQPKDEMVKVVIEMITGRLPPETVT
LPGKLIVRNSA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=300, Percent_Identity=25, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1789068, Length=340, Percent_Identity=28.8235294117647, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787948, Length=338, Percent_Identity=26.9230769230769, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790194, Length=320, Percent_Identity=25.9375, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790689, Length=354, Percent_Identity=24.0112994350282, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI48994940, Length=323, Percent_Identity=23.2198142414861, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789202, Length=278, Percent_Identity=24.1007194244604, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788474, Length=308, Percent_Identity=22.0779220779221, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786540, Length=296, Percent_Identity=23.6486486486486, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36700; Mature: 36700

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRRATAKNVADAAGVSKWTVIRAFTPGASITEESKRRVLQAAAALNYSPNLLARSLATN
CCCCCCHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LTHQVAVFVDDFANPQKLPFLETLTDRLQAEGLVAMLININNHFDHVRALLNADQRQVDA
HHHHHEEEECCCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHCCCHHHEEE
IIMFGTAFRSETLNDRRLGRSMPPMFVLARDSQIYGVPAVVCDAELALKEIVDHLYEKGY
EEEEECHHHHCCCCHHHCCCCCCCEEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHCCC
RRPGFMAGAPALSTALRRRHHFIDFWKQKGVDETAVISAERYSAQAGAESVRHYLQNTDA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHEEEHHHHHHHHHHHHHHHHHHCCCC
KDRVDVLMCENDILAIGALDEIRGQFGLRVPQDMAVIGFDNYELGGASVYGLSTYEQPKD
CCCEEEEEECCCEEEEECHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCHH
EMVKVVIEMITGRLPPETVTLPGKLIVRNSA
HHHHHHHHHHHCCCCCCEEECCCEEEEECCC
>Mature Secondary Structure
MRRRATAKNVADAAGVSKWTVIRAFTPGASITEESKRRVLQAAAALNYSPNLLARSLATN
CCCCCCHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LTHQVAVFVDDFANPQKLPFLETLTDRLQAEGLVAMLININNHFDHVRALLNADQRQVDA
HHHHHEEEECCCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHCCCHHHEEE
IIMFGTAFRSETLNDRRLGRSMPPMFVLARDSQIYGVPAVVCDAELALKEIVDHLYEKGY
EEEEECHHHHCCCCHHHCCCCCCCEEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHCCC
RRPGFMAGAPALSTALRRRHHFIDFWKQKGVDETAVISAERYSAQAGAESVRHYLQNTDA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHEEEHHHHHHHHHHHHHHHHHHCCCC
KDRVDVLMCENDILAIGALDEIRGQFGLRVPQDMAVIGFDNYELGGASVYGLSTYEQPKD
CCCEEEEEECCCEEEEECHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCHH
EMVKVVIEMITGRLPPETVTLPGKLIVRNSA
HHHHHHHHHHHCCCCCCEEECCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA