Click here to switch to the map view.

The map label for this gene is nodW [H]

Identifier: 13471099

GI number: 13471099

Start: 803852

End: 804592

Strand: Reverse

Name: nodW [H]

Synonym: mll0983

Alternate gene names: 13471099

Gene position: 804592-803852 (Counterclockwise)

Preceding gene: 13471100

Following gene: 13471092

Centisome position: 11.44

GC content: 57.09

Gene sequence:

>741_bases
ATGACGCCTTGTACAGTCGAGAAATGGAGGACGATCGTGACAGATGAGCAGTCGCTCCCGCGGAGATCGCCGCCGCTATC
TACGGAGCAGAAGACCGAAGTGAGGGAGCCGCTCGTCATTATTGTCGATGACGACGCTTCCGTCCGGCAGGCCGTCTCCG
AATTGATCCTTTCGGCGGGTCTGCAACCCGTCTGTTTTGCTTCGACCGGTGAATTGTTAGATGCGGATGTGTTGGACAAT
CCAGGTTGTCTGATCCTGGATGTGCGCATGCCGGGAGCAAGTGGTCTGGACCTTCAACATCGCCTGGCCCAACGGGGCAA
TCCAAAGCCCGTCATCTTTCTCACCGGCCATGGCGACATTCCGATGTCTGTACAGGCCATGAAGGCGGGGGCTGTCGACT
TTCTCACCAAGCCGGTGCGTGATCAAACGCTGCTTGACGCGGTCATTGCCGGGATTGCAATCGATGCCGTGCAACGGAAA
GAAGCCGCGATCGTCAAGCGAAACACTGAATTGTTCGCAGCGCTGACTCCTCGGGAGCGCCAGGTCTTGCGTGAGGTCGC
GCGAGGGCGAGTAAGCAAGCAAATTGCCTTCGACCTTGGCATAAGCGAGATCACCGTCAAGCTTCACCGCGGTAATGCCA
TGCGCAAGATGAAGGCTGCATCGATCGGCCAGCTGATCCAATCATGGGAAACACTGCCCGCTTCGGTTCGCGAGAGTGAA
ATGAATCGGCCATCGCTATAA

Upstream 100 bases:

>100_bases
GAAACGGAAAAGGCGCCATGACGAAGGGGGATTGCATCGTCTTCATTGTCGAGCACGGTGAACGAATCCGAGCACGCATA
GGCGCCTTAGCCGGCGCCCA

Downstream 100 bases:

>100_bases
TCCCGCGCCGCTCTCTCAGGCATTATGGGCGAGCCTGGAGCGAAGGTCACTCGTCGAAAGGCGCGTTTGCCACAAGATCG
ACGCTCGAGGGAGCCCGGTA

Product: transcriptional activator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTPCTVEKWRTIVTDEQSLPRRSPPLSTEQKTEVREPLVIIVDDDASVRQAVSELILSAGLQPVCFASTGELLDADVLDN
PGCLILDVRMPGASGLDLQHRLAQRGNPKPVIFLTGHGDIPMSVQAMKAGAVDFLTKPVRDQTLLDAVIAGIAIDAVQRK
EAAIVKRNTELFAALTPRERQVLREVARGRVSKQIAFDLGISEITVKLHRGNAMRKMKAASIGQLIQSWETLPASVRESE
MNRPSL

Sequences:

>Translated_246_residues
MTPCTVEKWRTIVTDEQSLPRRSPPLSTEQKTEVREPLVIIVDDDASVRQAVSELILSAGLQPVCFASTGELLDADVLDN
PGCLILDVRMPGASGLDLQHRLAQRGNPKPVIFLTGHGDIPMSVQAMKAGAVDFLTKPVRDQTLLDAVIAGIAIDAVQRK
EAAIVKRNTELFAALTPRERQVLREVARGRVSKQIAFDLGISEITVKLHRGNAMRKMKAASIGQLIQSWETLPASVRESE
MNRPSL
>Mature_245_residues
TPCTVEKWRTIVTDEQSLPRRSPPLSTEQKTEVREPLVIIVDDDASVRQAVSELILSAGLQPVCFASTGELLDADVLDNP
GCLILDVRMPGASGLDLQHRLAQRGNPKPVIFLTGHGDIPMSVQAMKAGAVDFLTKPVRDQTLLDAVIAGIAIDAVQRKE
AAIVKRNTELFAALTPRERQVLREVARGRVSKQIAFDLGISEITVKLHRGNAMRKMKAASIGQLIQSWETLPASVRESEM
NRPSL

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=102, Percent_Identity=39.2156862745098, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788521, Length=199, Percent_Identity=36.6834170854271, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790102, Length=185, Percent_Identity=33.5135135135135, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788222, Length=195, Percent_Identity=28.2051282051282, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 26903; Mature: 26771

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPCTVEKWRTIVTDEQSLPRRSPPLSTEQKTEVREPLVIIVDDDASVRQAVSELILSAG
CCCCCHHHHHHHHCCCHHCCCCCCCCCCCHHHHCCCCEEEEECCCHHHHHHHHHHHHHCC
LQPVCFASTGELLDADVLDNPGCLILDVRMPGASGLDLQHRLAQRGNPKPVIFLTGHGDI
CCCEEEECCCCEEECCCCCCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCEEEEECCCCC
PMSVQAMKAGAVDFLTKPVRDQTLLDAVIAGIAIDAVQRKEAAIVKRNTELFAALTPRER
CCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHH
QVLREVARGRVSKQIAFDLGISEITVKLHRGNAMRKMKAASIGQLIQSWETLPASVRESE
HHHHHHHHHHHHHHHHHHCCHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHC
MNRPSL
CCCCCC
>Mature Secondary Structure 
TPCTVEKWRTIVTDEQSLPRRSPPLSTEQKTEVREPLVIIVDDDASVRQAVSELILSAG
CCCCHHHHHHHHCCCHHCCCCCCCCCCCHHHHCCCCEEEEECCCHHHHHHHHHHHHHCC
LQPVCFASTGELLDADVLDNPGCLILDVRMPGASGLDLQHRLAQRGNPKPVIFLTGHGDI
CCCEEEECCCCEEECCCCCCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCEEEEECCCCC
PMSVQAMKAGAVDFLTKPVRDQTLLDAVIAGIAIDAVQRKEAAIVKRNTELFAALTPRER
CCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHH
QVLREVARGRVSKQIAFDLGISEITVKLHRGNAMRKMKAASIGQLIQSWETLPASVRESE
HHHHHHHHHHHHHHHHHHCCHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHC
MNRPSL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]