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The map label for this gene is pucI [H]

Identifier: 13470806

GI number: 13470806

Start: 470766

End: 472223

Strand: Direct

Name: pucI [H]

Synonym: mlr0604

Alternate gene names: 13470806

Gene position: 470766-472223 (Clockwise)

Preceding gene: 13470805

Following gene: 13470812

Centisome position: 6.69

GC content: 61.8

Gene sequence:

>1458_bases
GTGACCATACAAGGCGCATCGCCTGACCTGTACAACGAGGATCTCGCTCCGGCCAAAGTTCGAAACTGGGGAGCATTCTC
GATCTTCAACGTCTGGACGTCGGACGTTCACAGCCTGTGGGGCTACTATCTCGCCGCCAGCCTTTTCCTGTTCTGCGGCG
GCTTCGTCAATTTCATCATCGCCATCGGCATCGGCTCGCTGATCATCTACGCGCTGATGAACATGGTCGGTAACGCCGGC
GTCAAAACCGGCGTGCCCTACCCCGTGCTTGCCCGTGCCTCCTTCGGCATCTGGGGCGCCAACATCCCGGCATTGGTGCG
CGCCATCGTCGCCTGCTTCTGGTACGGTGCGCAAACGGCGGCCGCCTCCGGCGCCATCGTGGCATTGTTGACCCGCCTGC
AATGGTTCGACGAATTCAACAAGACCTCGCATCTTCTCGGCCATTCCACCCTGGAAGTGATCTGCTTCGTCGTCATCTGG
GCGCTGCAGCTGCTGATCATCCAGAAGGGCATGGAAACGGTGCGCCGCTTCCAGGATTGGGCGGGGCCTGCCGTCTGGGT
GATGATGCTGCTCCTGGCCATCTATCTCTGCGTCAAATCGGGGACCTTCGCCTTTACCAGCGACATTCCGATGGACGTGC
TGCGCGAAAAGACCGCCGACGCCGGCATTCCGGGCGATCCCGGATCGTGGACGGCGCTCTTCGGGGTCGCGGCGATCTGG
GTGACCTATTTCTCGGCGCTATACCTCAATTTTTGCGACTTCGCCCGCTACGCACCGGACAAGGCGGCATTGCGCAAGGG
CAACATCTGGGGCCTGCCGGTCAACCTCATCCTGTTTTCGCTGGTCGCCGGCGTCACCACCATTGCCGCCTATGACGTCT
ATCACGAGGTGCTGCTGCACCCCGACCAGATCTCGGCCAAGTTCGACAGCTGGTTCCTGGCTGCGCTCGCCGCCCTGACC
TTCGCGGTGGCCACGCTCGGCATCAACGTCGTCGCCAACTTCGTCTCGCCGGCCTTCGACTTCTCCAACGTCTTTCCGCG
TCAGATCGACTTCAAGAAGGGCGGCTACATCGCAGCACTTATCGCACTTGTGCTCTATCCCTTCGCGCCGTGGGAAGGCA
GTGCCGCGCATTTCGTCGGCATCATCGGCGCGACGATGGGGCCTATCTTCGGCGTCATGATGGTCGACTATTACCTGATC
CGCAAAGGCGAGGTCGACGTCGAGGCGCTGTATCGTGAGGACGGCGAGTTCCGCTTCCAGGGCGGCTGGCACGTCAATGC
CTTCATCGCCGCCGGCATAGGCGCTGTGTTCTCGTCGATCCTGCCGAACTTCACCAACTGGCTGCCATCCTGGTGGGGCG
TCTATGGCTGGTTCTTCGGCGTGGCGATTGCCGGAACCATCTACTACGTGCTGCGCACCGTCGCCGTGGGTGCGGGCGCC
AAGACGGCAAAGGCTTGA

Upstream 100 bases:

>100_bases
AAAGCCGGCAAATGGGTTGCTTGTCGGTTCCCCAGCGTCAGGCTTAACCTGCACGGCCATCGCGTGGAGGCGCGTGGCCA
ATCAAGGGGAGGATTTTGCA

Downstream 100 bases:

>100_bases
AAAGAGGGAGTAGGCAGTAGGAGTTAGGGAATACAGACCCTTCCCTACTGCCTACTGCCTACTGCCCACCATCTCAGCCA
ACTTGCGCACCGTGTTCCAG

Product: permease

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MTIQGASPDLYNEDLAPAKVRNWGAFSIFNVWTSDVHSLWGYYLAASLFLFCGGFVNFIIAIGIGSLIIYALMNMVGNAG
VKTGVPYPVLARASFGIWGANIPALVRAIVACFWYGAQTAAASGAIVALLTRLQWFDEFNKTSHLLGHSTLEVICFVVIW
ALQLLIIQKGMETVRRFQDWAGPAVWVMMLLLAIYLCVKSGTFAFTSDIPMDVLREKTADAGIPGDPGSWTALFGVAAIW
VTYFSALYLNFCDFARYAPDKAALRKGNIWGLPVNLILFSLVAGVTTIAAYDVYHEVLLHPDQISAKFDSWFLAALAALT
FAVATLGINVVANFVSPAFDFSNVFPRQIDFKKGGYIAALIALVLYPFAPWEGSAAHFVGIIGATMGPIFGVMMVDYYLI
RKGEVDVEALYREDGEFRFQGGWHVNAFIAAGIGAVFSSILPNFTNWLPSWWGVYGWFFGVAIAGTIYYVLRTVAVGAGA
KTAKA

Sequences:

>Translated_485_residues
MTIQGASPDLYNEDLAPAKVRNWGAFSIFNVWTSDVHSLWGYYLAASLFLFCGGFVNFIIAIGIGSLIIYALMNMVGNAG
VKTGVPYPVLARASFGIWGANIPALVRAIVACFWYGAQTAAASGAIVALLTRLQWFDEFNKTSHLLGHSTLEVICFVVIW
ALQLLIIQKGMETVRRFQDWAGPAVWVMMLLLAIYLCVKSGTFAFTSDIPMDVLREKTADAGIPGDPGSWTALFGVAAIW
VTYFSALYLNFCDFARYAPDKAALRKGNIWGLPVNLILFSLVAGVTTIAAYDVYHEVLLHPDQISAKFDSWFLAALAALT
FAVATLGINVVANFVSPAFDFSNVFPRQIDFKKGGYIAALIALVLYPFAPWEGSAAHFVGIIGATMGPIFGVMMVDYYLI
RKGEVDVEALYREDGEFRFQGGWHVNAFIAAGIGAVFSSILPNFTNWLPSWWGVYGWFFGVAIAGTIYYVLRTVAVGAGA
KTAKA
>Mature_484_residues
TIQGASPDLYNEDLAPAKVRNWGAFSIFNVWTSDVHSLWGYYLAASLFLFCGGFVNFIIAIGIGSLIIYALMNMVGNAGV
KTGVPYPVLARASFGIWGANIPALVRAIVACFWYGAQTAAASGAIVALLTRLQWFDEFNKTSHLLGHSTLEVICFVVIWA
LQLLIIQKGMETVRRFQDWAGPAVWVMMLLLAIYLCVKSGTFAFTSDIPMDVLREKTADAGIPGDPGSWTALFGVAAIWV
TYFSALYLNFCDFARYAPDKAALRKGNIWGLPVNLILFSLVAGVTTIAAYDVYHEVLLHPDQISAKFDSWFLAALAALTF
AVATLGINVVANFVSPAFDFSNVFPRQIDFKKGGYIAALIALVLYPFAPWEGSAAHFVGIIGATMGPIFGVMMVDYYLIR
KGEVDVEALYREDGEFRFQGGWHVNAFIAAGIGAVFSSILPNFTNWLPSWWGVYGWFFGVAIAGTIYYVLRTVAVGAGAK
TAKA

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=485, Percent_Identity=28.2474226804124, Blast_Score=155, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6319495, Length=462, Percent_Identity=23.5930735930736, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6322219, Length=444, Percent_Identity=22.972972972973, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319429, Length=461, Percent_Identity=23.644251626898, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324766, Length=444, Percent_Identity=22.972972972973, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323266, Length=446, Percent_Identity=24.2152466367713, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324645, Length=444, Percent_Identity=21.6216216216216, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 52890; Mature: 52759

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIQGASPDLYNEDLAPAKVRNWGAFSIFNVWTSDVHSLWGYYLAASLFLFCGGFVNFII
CCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIGIGSLIIYALMNMVGNAGVKTGVPYPVLARASFGIWGANIPALVRAIVACFWYGAQTA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHH
AASGAIVALLTRLQWFDEFNKTSHLLGHSTLEVICFVVIWALQLLIIQKGMETVRRFQDW
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGPAVWVMMLLLAIYLCVKSGTFAFTSDIPMDVLREKTADAGIPGDPGSWTALFGVAAIW
CCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
VTYFSALYLNFCDFARYAPDKAALRKGNIWGLPVNLILFSLVAGVTTIAAYDVYHEVLLH
HHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PDQISAKFDSWFLAALAALTFAVATLGINVVANFVSPAFDFSNVFPRQIDFKKGGYIAAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
IALVLYPFAPWEGSAAHFVGIIGATMGPIFGVMMVDYYLIRKGEVDVEALYREDGEFRFQ
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEE
GGWHVNAFIAAGIGAVFSSILPNFTNWLPSWWGVYGWFFGVAIAGTIYYVLRTVAVGAGA
CCCEEHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KTAKA
CCCCC
>Mature Secondary Structure 
TIQGASPDLYNEDLAPAKVRNWGAFSIFNVWTSDVHSLWGYYLAASLFLFCGGFVNFII
CCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]