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The map label for this gene is gcvA [C]

Identifier: 13470805

GI number: 13470805

Start: 469654

End: 470556

Strand: Direct

Name: gcvA [C]

Synonym: mlr0603

Alternate gene names: 13470805

Gene position: 469654-470556 (Clockwise)

Preceding gene: 13470796

Following gene: 13470806

Centisome position: 6.67

GC content: 62.35

Gene sequence:

>903_bases
ATGGCCCTTCCCTCACTCAACGGCATGCGCGCTTTCGAGGCCGCCGCACGTCTCGGCAGCATCAAGGATGCAGCCGAGGA
GTTGCACCTGACGCCCTCGGCCATCAGCCGCCATATCCGTGCGCTTGAAAGAAATCTCGGCCAGGATTTGTTCGAACGCG
GCTTCCGCCAGATAACGCCGACCATCAGGGGCTCCTACTATGCGCGCAGCCTCTCCGAAGCGTTCGAGGCCATCTGGCGC
GCCACCGACGATGTCAGCCTCGCCGATGGCCCAAGCCACGGCAGGACCCAACGCGTCAGGGTCTTGTGCGTCCCGGCGGT
CCTGAACCTTTGGCTGGCGGACCGGTTGCCGAATTTTCGTCGGCTGCATCCAGCGGTGGAACTGGAGATCTCGACATCAG
GCAAGCGCGCCAACTTCGATTTGGCCATTGTCGACGAGTTCGTCTACAAGGCCGGCCCGGCCTTGACGCTTTTGATCCCG
CTGGTTCTGACGCCGGTCTGCGCACCCTCATTGCTCGACGGGCCCGTGCCGCTGCGATCGCCTGCCGATCTGGTCAACCA
TCATCTTATCCACGAATGCGAGAGCATGAGGTGGAAGCGTTGGCTGGAGCAGGAAGGCGTTCTGGACACGACGCCGAAAT
CCAGCACGACGCTGGATGACTGCACGCTCATCATGCGCGAGGCGATCAATGGCGCCGGAATTGCGCTCGCCGATACGATG
ATGGCGCAGGATTTTCTGCAGCAAGGCAAACTCGTCGCCCCGTTTCCTGCCCGCCACACCTACCCGGCCGGCATTTACCT
GCATCAGCGCCGCAGCATCGGCAACAAGCCAGGGACCGGCCTGTTTCAGGACTGGCTGTTGTCGGAAGTCGAGGACCACA
AGCGGGTCATGGCTATCGCCTAG

Upstream 100 bases:

>100_bases
GCTCTTGCCTTTTGGGTAGCGCCGAACAGGCCAGGCAATCAAACGCCAATTTTACCCAAGTCGATTGACAAAAACTCAAC
TGACAATTCATGATTAGCCA

Downstream 100 bases:

>100_bases
GCGGAGAGGCGGTACCCAGATTCGAATCACCCGAGCTGTTGGTATTCAACAGCGGCCCACCATTGTGCCCCCTCGATAAT
CGCGGTTCGCATTGAATATC

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MALPSLNGMRAFEAAARLGSIKDAAEELHLTPSAISRHIRALERNLGQDLFERGFRQITPTIRGSYYARSLSEAFEAIWR
ATDDVSLADGPSHGRTQRVRVLCVPAVLNLWLADRLPNFRRLHPAVELEISTSGKRANFDLAIVDEFVYKAGPALTLLIP
LVLTPVCAPSLLDGPVPLRSPADLVNHHLIHECESMRWKRWLEQEGVLDTTPKSSTTLDDCTLIMREAINGAGIALADTM
MAQDFLQQGKLVAPFPARHTYPAGIYLHQRRSIGNKPGTGLFQDWLLSEVEDHKRVMAIA

Sequences:

>Translated_300_residues
MALPSLNGMRAFEAAARLGSIKDAAEELHLTPSAISRHIRALERNLGQDLFERGFRQITPTIRGSYYARSLSEAFEAIWR
ATDDVSLADGPSHGRTQRVRVLCVPAVLNLWLADRLPNFRRLHPAVELEISTSGKRANFDLAIVDEFVYKAGPALTLLIP
LVLTPVCAPSLLDGPVPLRSPADLVNHHLIHECESMRWKRWLEQEGVLDTTPKSSTTLDDCTLIMREAINGAGIALADTM
MAQDFLQQGKLVAPFPARHTYPAGIYLHQRRSIGNKPGTGLFQDWLLSEVEDHKRVMAIA
>Mature_299_residues
ALPSLNGMRAFEAAARLGSIKDAAEELHLTPSAISRHIRALERNLGQDLFERGFRQITPTIRGSYYARSLSEAFEAIWRA
TDDVSLADGPSHGRTQRVRVLCVPAVLNLWLADRLPNFRRLHPAVELEISTSGKRANFDLAIVDEFVYKAGPALTLLIPL
VLTPVCAPSLLDGPVPLRSPADLVNHHLIHECESMRWKRWLEQEGVLDTTPKSSTTLDDCTLIMREAINGAGIALADTMM
AQDFLQQGKLVAPFPARHTYPAGIYLHQRRSIGNKPGTGLFQDWLLSEVEDHKRVMAIA

Specific function: Regulatory Protein For The Glycine Cleavage System Operon (Gcv). Mediates Activation Of Gcv By Glycine And Repression By Purines. Gcva Is Negatively Autoregulated. Bind To Three Sites Upstream Of The Gcv Promoter. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=30.1369863013699, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1786448, Length=300, Percent_Identity=32.3333333333333, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788706, Length=285, Percent_Identity=27.3684210526316, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI145693193, Length=311, Percent_Identity=24.7588424437299, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787128, Length=257, Percent_Identity=25.6809338521401, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33300; Mature: 33169

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALPSLNGMRAFEAAARLGSIKDAAEELHLTPSAISRHIRALERNLGQDLFERGFRQITP
CCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
TIRGSYYARSLSEAFEAIWRATDDVSLADGPSHGRTQRVRVLCVPAVLNLWLADRLPNFR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCHH
RLHPAVELEISTSGKRANFDLAIVDEFVYKAGPALTLLIPLVLTPVCAPSLLDGPVPLRS
HHCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHCCCCCCCCC
PADLVNHHLIHECESMRWKRWLEQEGVLDTTPKSSTTLDDCTLIMREAINGAGIALADTM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHH
MAQDFLQQGKLVAPFPARHTYPAGIYLHQRRSIGNKPGTGLFQDWLLSEVEDHKRVMAIA
HHHHHHHCCCEECCCCCCCCCCCCEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
ALPSLNGMRAFEAAARLGSIKDAAEELHLTPSAISRHIRALERNLGQDLFERGFRQITP
CCCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
TIRGSYYARSLSEAFEAIWRATDDVSLADGPSHGRTQRVRVLCVPAVLNLWLADRLPNFR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCHH
RLHPAVELEISTSGKRANFDLAIVDEFVYKAGPALTLLIPLVLTPVCAPSLLDGPVPLRS
HHCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHCCCCCCCCC
PADLVNHHLIHECESMRWKRWLEQEGVLDTTPKSSTTLDDCTLIMREAINGAGIALADTM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHH
MAQDFLQQGKLVAPFPARHTYPAGIYLHQRRSIGNKPGTGLFQDWLLSEVEDHKRVMAIA
HHHHHHHCCCEECCCCCCCCCCCCEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]