Click here to switch to the map view.

The map label for this gene is garR [C]

Identifier: 13470761

GI number: 13470761

Start: 406521

End: 407390

Strand: Direct

Name: garR [C]

Synonym: mlr0552

Alternate gene names: 13470761

Gene position: 406521-407390 (Clockwise)

Preceding gene: 13470760

Following gene: 13470762

Centisome position: 5.78

GC content: 62.41

Gene sequence:

>870_bases
ATGGCATCTGTGGCATTTCTCGGTCTTGGCGTGATGGGCTACCCCATGGCCGCGCACCTCAGGAACAAGGGTGATCACGA
CGTCACCGTCTACAACCGCACCAAGACCAAGGCCGAGCAATGGGTCGACCAGCATGGCGGCAGCCTTGCGGTGACGCCGG
CAGACGCAGCTAAGGACAAGGATTTCGTCTTTTCCTGCGTCGGCAATGACGACGATCTGCGCTCGGTGACGACTGGTCCC
GAAGGCGCCTTCAAATCGATGAAGAAAGGTTCGGTCTTCATCGACAACACCACTGCCTCGGCGGAAGTCGCGCGCGAACT
GGCGGCAGCAGCGGAGGCAGGTGGATTTTCGTTTCTCGACGCGCCGGTCTCCGGTGGCCAGGCCGGCGCGGAGAACGGCG
TGCTGACCGTCATGGTCGGCGGCGATCAGGCTGCCTACGACAGAGCGAGGCCCGTTATCGATGCCTATGCCCGCATGGTC
GGGCTGATGGGGTCGGCGGGCGCTGGCCAGCTCACCAAGATGATCAACCAGATCTGCATCGCCGGGCTGGTGCAGGGCCT
GTCGGAAGGCATCCATTTCGGCAAAAAGGCCGGGCTCGACATCGAGAAGGTGATCGAGGTGATTTCCAAGGGTGCCGCCG
GTTCCTGGCAGATGGAAAACCGCCACAAGACGATGAATGCCGGCAAATATGATTTCGGCTTTGCCGTCGACTGGATGCGC
AAGGATCTTGGCATCTGCCTGGCGGAGGCCGACCGCAACGGCGCCAAGCTGCCGGTGACCGCGCTTGTCGACCAGTTCTA
CAAGGATGTGCAGGCCATGGGCGGCAAGCGCTGGGACACGTCCTCGCTGCTCGCGCGCCTGGAGAAGTAG

Upstream 100 bases:

>100_bases
TTCGGCTGTGCCAGTCGATCGCACCACGCGCGTCTTGACGAATTAAGCGCAGGGCCTAGAGCTTTCCCAGCAATTTCGTT
TCCGTCTGAAGGGCAAGCTC

Downstream 100 bases:

>100_bases
GGGGCCATGTCCCTGCCCGCCCCAGACTGGACGGCGCACGACATCATCGCACATCTGCGCTCAATCGGCACCGACGAAAA
CCGCGCCGGCATGGCGCGGT

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MASVAFLGLGVMGYPMAAHLRNKGDHDVTVYNRTKTKAEQWVDQHGGSLAVTPADAAKDKDFVFSCVGNDDDLRSVTTGP
EGAFKSMKKGSVFIDNTTASAEVARELAAAAEAGGFSFLDAPVSGGQAGAENGVLTVMVGGDQAAYDRARPVIDAYARMV
GLMGSAGAGQLTKMINQICIAGLVQGLSEGIHFGKKAGLDIEKVIEVISKGAAGSWQMENRHKTMNAGKYDFGFAVDWMR
KDLGICLAEADRNGAKLPVTALVDQFYKDVQAMGGKRWDTSSLLARLEK

Sequences:

>Translated_289_residues
MASVAFLGLGVMGYPMAAHLRNKGDHDVTVYNRTKTKAEQWVDQHGGSLAVTPADAAKDKDFVFSCVGNDDDLRSVTTGP
EGAFKSMKKGSVFIDNTTASAEVARELAAAAEAGGFSFLDAPVSGGQAGAENGVLTVMVGGDQAAYDRARPVIDAYARMV
GLMGSAGAGQLTKMINQICIAGLVQGLSEGIHFGKKAGLDIEKVIEVISKGAAGSWQMENRHKTMNAGKYDFGFAVDWMR
KDLGICLAEADRNGAKLPVTALVDQFYKDVQAMGGKRWDTSSLLARLEK
>Mature_288_residues
ASVAFLGLGVMGYPMAAHLRNKGDHDVTVYNRTKTKAEQWVDQHGGSLAVTPADAAKDKDFVFSCVGNDDDLRSVTTGPE
GAFKSMKKGSVFIDNTTASAEVARELAAAAEAGGFSFLDAPVSGGQAGAENGVLTVMVGGDQAAYDRARPVIDAYARMVG
LMGSAGAGQLTKMINQICIAGLVQGLSEGIHFGKKAGLDIEKVIEVISKGAAGSWQMENRHKTMNAGKYDFGFAVDWMRK
DLGICLAEADRNGAKLPVTALVDQFYKDVQAMGGKRWDTSSLLARLEK

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=280, Percent_Identity=26.4285714285714, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI23308751, Length=293, Percent_Identity=29.6928327645051, Blast_Score=131, Evalue=6e-31,
Organism=Escherichia coli, GI145693186, Length=286, Percent_Identity=33.9160839160839, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI1786719, Length=286, Percent_Identity=32.1678321678322, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1790315, Length=294, Percent_Identity=30.952380952381, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1789092, Length=230, Percent_Identity=30.8695652173913, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788341, Length=213, Percent_Identity=29.1079812206573, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17557316, Length=276, Percent_Identity=26.8115942028986, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24655230, Length=296, Percent_Identity=28.0405405405405, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI19922568, Length=296, Percent_Identity=28.0405405405405, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI28574115, Length=282, Percent_Identity=21.9858156028369, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30575; Mature: 30444

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASVAFLGLGVMGYPMAAHLRNKGDHDVTVYNRTKTKAEQWVDQHGGSLAVTPADAAKDK
CCCHHHHHHHHHHCHHHHHHCCCCCCCEEEEECCHHHHHHHHHHCCCEEEECCCCCCCCC
DFVFSCVGNDDDLRSVTTGPEGAFKSMKKGSVFIDNTTASAEVARELAAAAEAGGFSFLD
CEEEEECCCCCCHHHCCCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEEC
APVSGGQAGAENGVLTVMVGGDQAAYDRARPVIDAYARMVGLMGSAGAGQLTKMINQICI
CCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
AGLVQGLSEGIHFGKKAGLDIEKVIEVISKGAAGSWQMENRHKTMNAGKYDFGFAVDWMR
HHHHHHHHHHHHCCHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHH
KDLGICLAEADRNGAKLPVTALVDQFYKDVQAMGGKRWDTSSLLARLEK
HHHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
ASVAFLGLGVMGYPMAAHLRNKGDHDVTVYNRTKTKAEQWVDQHGGSLAVTPADAAKDK
CCHHHHHHHHHHCHHHHHHCCCCCCCEEEEECCHHHHHHHHHHCCCEEEECCCCCCCCC
DFVFSCVGNDDDLRSVTTGPEGAFKSMKKGSVFIDNTTASAEVARELAAAAEAGGFSFLD
CEEEEECCCCCCHHHCCCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEEC
APVSGGQAGAENGVLTVMVGGDQAAYDRARPVIDAYARMVGLMGSAGAGQLTKMINQICI
CCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
AGLVQGLSEGIHFGKKAGLDIEKVIEVISKGAAGSWQMENRHKTMNAGKYDFGFAVDWMR
HHHHHHHHHHHHCCHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHH
KDLGICLAEADRNGAKLPVTALVDQFYKDVQAMGGKRWDTSSLLARLEK
HHHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA