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The map label for this gene is moaA

Identifier: 13470752

GI number: 13470752

Start: 399557

End: 400552

Strand: Direct

Name: moaA

Synonym: mlr0541

Alternate gene names: 13470752

Gene position: 399557-400552 (Clockwise)

Preceding gene: 13470749

Following gene: 13470753

Centisome position: 5.68

GC content: 63.05

Gene sequence:

>996_bases
ATGAACATGATCGACCCCTTCGGTCGCACGATCAGCTATCTCAGAGTGTCGGTCACCGACCGCTGCGATTTTCGCTGCAC
CTATTGCATGGCCGAAGACATGGCCTTCCTGCCGAAGAAGGACCTGTTGTCGCTGGAAGAGCTGGACCGGCTCTGCAGCG
TCTTCATCGAGAAGGGCGTCAGACGGCTGCGGCTCACCGGCGGCGAGCCACTGGTGCGCAAAAACATCATGCATCTGGTG
CGCCAGCTCTCGCGCCACCTCGACAGCGGCGCGCTGGAAGAACTGACGCTGACCACCAACGGCTCGCAGCTGTCGCGCTT
CGCCGCCGAGCTCGCCGATTGCGGCGTCAAGCGCATCAACGTCTCGCTCGACACGCTCGATGCCGACAAGTTCCACCAGA
TCACCCGCTGGGGCCATCTCGACAAGGTCATGCAAGGCATCGATGCCGCTCAGGCGGCCGGGCTGAAGGTCAAGCTCAAC
GCGGTGGCGTTGAAGGATTTCAACGACGCCGAACTGCCCGACATGATGCGCTGGGCGCATGGCCGCGGCATGGATCTCAC
CGTCATCGAAACCATGCCGATGGGCGAGATCGACGCCGATCGCACCGACCAGTATCTGCCGCTGTCGCTGCTGCGCGCCT
CGCTGGAGCGTCAGTTCACGCTGACCGACATTCCCTTCAAGACCGGTGGCCCTGCCCGATACGTCCACGTCGCCGAGACC
GGCGGAAAGCTCGGTTTCATCACACCGATGACGCATAATTTCTGCGAAAGCTGCAACCGCGTCCGGCTGACCTGCACCGG
TACGCTCTATATGTGCCTTGGCCAGGAAGACGCGGCCGACCTGCGCGCGCCGTTGCGTGCATCCGAAGGCAACGAACTTG
TCGCCGACGCCATCGACGAGGCCATCGGCCGCAAGCCGAAAGGCCATGATTTCATCATCGACCGCCGCACCAGCCGTCCC
TCGGTTTCCCGGCATATGAGCGTCACCGGCGGCTGA

Upstream 100 bases:

>100_bases
GTTACCAAAGATCGAGAAGCGGTATCTTGAATGGCGGGCTGGATCGTATCACATAGCTGTATATAACGGCGCCCGGTAAC
GGCCACGGAAACGCTCGAAC

Downstream 100 bases:

>100_bases
TTTTTTCTCCGATTTGGTGCAAGATTATCTTTGGTCACAAGGGCCAAGGGGGCATCGTGCTGCGTCTTTATCGTATCGGC
TTGTCGGCGCTGACCACAGT

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MNMIDPFGRTISYLRVSVTDRCDFRCTYCMAEDMAFLPKKDLLSLEELDRLCSVFIEKGVRRLRLTGGEPLVRKNIMHLV
RQLSRHLDSGALEELTLTTNGSQLSRFAAELADCGVKRINVSLDTLDADKFHQITRWGHLDKVMQGIDAAQAAGLKVKLN
AVALKDFNDAELPDMMRWAHGRGMDLTVIETMPMGEIDADRTDQYLPLSLLRASLERQFTLTDIPFKTGGPARYVHVAET
GGKLGFITPMTHNFCESCNRVRLTCTGTLYMCLGQEDAADLRAPLRASEGNELVADAIDEAIGRKPKGHDFIIDRRTSRP
SVSRHMSVTGG

Sequences:

>Translated_331_residues
MNMIDPFGRTISYLRVSVTDRCDFRCTYCMAEDMAFLPKKDLLSLEELDRLCSVFIEKGVRRLRLTGGEPLVRKNIMHLV
RQLSRHLDSGALEELTLTTNGSQLSRFAAELADCGVKRINVSLDTLDADKFHQITRWGHLDKVMQGIDAAQAAGLKVKLN
AVALKDFNDAELPDMMRWAHGRGMDLTVIETMPMGEIDADRTDQYLPLSLLRASLERQFTLTDIPFKTGGPARYVHVAET
GGKLGFITPMTHNFCESCNRVRLTCTGTLYMCLGQEDAADLRAPLRASEGNELVADAIDEAIGRKPKGHDFIIDRRTSRP
SVSRHMSVTGG
>Mature_331_residues
MNMIDPFGRTISYLRVSVTDRCDFRCTYCMAEDMAFLPKKDLLSLEELDRLCSVFIEKGVRRLRLTGGEPLVRKNIMHLV
RQLSRHLDSGALEELTLTTNGSQLSRFAAELADCGVKRINVSLDTLDADKFHQITRWGHLDKVMQGIDAAQAAGLKVKLN
AVALKDFNDAELPDMMRWAHGRGMDLTVIETMPMGEIDADRTDQYLPLSLLRASLERQFTLTDIPFKTGGPARYVHVAET
GGKLGFITPMTHNFCESCNRVRLTCTGTLYMCLGQEDAADLRAPLRASEGNELVADAIDEAIGRKPKGHDFIIDRRTSRP
SVSRHMSVTGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=324, Percent_Identity=35.8024691358025, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI22027536, Length=324, Percent_Identity=35.8024691358025, Blast_Score=198, Evalue=5e-51,
Organism=Escherichia coli, GI1786999, Length=333, Percent_Identity=33.3333333333333, Blast_Score=194, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI32566331, Length=334, Percent_Identity=35.9281437125749, Blast_Score=210, Evalue=6e-55,
Organism=Caenorhabditis elegans, GI32566329, Length=313, Percent_Identity=36.741214057508, Blast_Score=205, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17561210, Length=313, Percent_Identity=35.7827476038339, Blast_Score=200, Evalue=8e-52,
Organism=Drosophila melanogaster, GI28574490, Length=312, Percent_Identity=38.7820512820513, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI28574488, Length=312, Percent_Identity=38.7820512820513, Blast_Score=206, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_RHILO (Q98MK6)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102321.1
- ProteinModelPortal:   Q98MK6
- SMR:   Q98MK6
- GeneID:   1224984
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr0541
- NMPDR:   fig|266835.1.peg.426
- HOGENOM:   HBG529885
- OMA:   FFRAMSR
- ProtClustDB:   PRK00164
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 36920; Mature: 36920

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 15-15 BINDING 28-28 BINDING 64-64 BINDING 68-68 BINDING 98-98 BINDING 122-122 BINDING 158-158 BINDING 192-192

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMIDPFGRTISYLRVSVTDRCDFRCTYCMAEDMAFLPKKDLLSLEELDRLCSVFIEKGV
CCCCCCHHHHEEHEEEEECCCCCCEEEEEEHHHHHHCCCHHCCCHHHHHHHHHHHHHHCH
RRLRLTGGEPLVRKNIMHLVRQLSRHLDSGALEELTLTTNGSQLSRFAAELADCGVKRIN
HEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEE
VSLDTLDADKFHQITRWGHLDKVMQGIDAAQAAGLKVKLNAVALKDFNDAELPDMMRWAH
EEEECCCCHHHHHHHHHCCHHHHHHHCCHHHHCCEEEEEEEEEEECCCCCCCHHHHHHHC
GRGMDLTVIETMPMGEIDADRTDQYLPLSLLRASLERQFTLTDIPFKTGGPARYVHVAET
CCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCEEEEEEEEC
GGKLGFITPMTHNFCESCNRVRLTCTGTLYMCLGQEDAADLRAPLRASEGNELVADAIDE
CCCEEEECCCHHHHHHCCCCEEEEEEEEEEEEECCCCHHHHHCCCCCCCCCHHHHHHHHH
AIGRKPKGHDFIIDRRTSRPSVSRHMSVTGG
HHCCCCCCCCEEEECCCCCCCHHHCEEECCC
>Mature Secondary Structure
MNMIDPFGRTISYLRVSVTDRCDFRCTYCMAEDMAFLPKKDLLSLEELDRLCSVFIEKGV
CCCCCCHHHHEEHEEEEECCCCCCEEEEEEHHHHHHCCCHHCCCHHHHHHHHHHHHHHCH
RRLRLTGGEPLVRKNIMHLVRQLSRHLDSGALEELTLTTNGSQLSRFAAELADCGVKRIN
HEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEE
VSLDTLDADKFHQITRWGHLDKVMQGIDAAQAAGLKVKLNAVALKDFNDAELPDMMRWAH
EEEECCCCHHHHHHHHHCCHHHHHHHCCHHHHCCEEEEEEEEEEECCCCCCCHHHHHHHC
GRGMDLTVIETMPMGEIDADRTDQYLPLSLLRASLERQFTLTDIPFKTGGPARYVHVAET
CCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCEEEEEEEEC
GGKLGFITPMTHNFCESCNRVRLTCTGTLYMCLGQEDAADLRAPLRASEGNELVADAIDE
CCCEEEECCCHHHHHHCCCCEEEEEEEEEEEEECCCCHHHHHCCCCCCCCCHHHHHHHHH
AIGRKPKGHDFIIDRRTSRPSVSRHMSVTGG
HHCCCCCCCCEEEECCCCCCCHHHCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968