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The map label for this gene is gph

Identifier: 13470740

GI number: 13470740

Start: 390028

End: 390711

Strand: Direct

Name: gph

Synonym: mlr0528

Alternate gene names: 13470740

Gene position: 390028-390711 (Clockwise)

Preceding gene: 13470736

Following gene: 13470741

Centisome position: 5.54

GC content: 64.77

Gene sequence:

>684_bases
ATGAGTCGCCCGATCATCGTGTTCGACCTCGACGGAACGCTGATTGACACAGCGCCGGACCTGCTCGACAGCCTCAACCA
CAGCCTGGCCGCCAGCGAACTCACGGCTGTCGACGAGGCCGGCTTCAGGCGCTTCGTCGGCCATGGCGGCCGCGTGATGA
TAGAGCGGGCGCACGCCGCCCAGCAGCGCTCGCTCGACGTTGCCGAGCACGACCGGCTGCTAAAACTGTTCCTCGATCAT
TACACCGACAACATCCCCGGCAAATCGCGCCCCTACCCCGGTGTCATCGAGGCGATCGCGCGCTTCGAGAAGGCTGGCTA
TCTCCTGGCCATTTGCACCAACAAATACGAAGCCAACTCGCTGGCGCTGATCGAAGCGCTTGGCTTGACCAGGCATTTCG
CGGCTATCGCCGGCCAGGACACGTTCGCCTTCCGCAAGCCCGACCCGCGCCACCTGACGGAAACCATCCGGCTGGCCGGC
GGCGATGCGCACCGCGCCCTGATGGTCGGCGATTCGCAAACCGACATCGACACCGCCAAGGCCGCCGGCATTCCGGTGGT
GGCGGTCGATTTCGGCTACACCGACCGGCATGTCCGCGAATTCGAACCTTCGGCGGTGATCTCGCATTTCGACGCATTGA
CCGTGGAATTGGCGGAGCGGCTGATCCGCGCCGCGGGACACTGA

Upstream 100 bases:

>100_bases
CAAAGCCTTTGATCATTGGGCGCCTGTCCACCGCTTTGCTTGCCTTGACGCGCCGCAGCCGCTATCGCCTGCCAATGCAC
AAACCTGCGCAGGATGGATC

Downstream 100 bases:

>100_bases
GCGAACAGCAACAAGAATACCGCCGGGCTCTGAAATGGCGCAGAATCCGATCTCTGAAAGAACGCCGGAGAGCGCCTTGA
CTTAACCGGCCGGCCTCCCT

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MSRPIIVFDLDGTLIDTAPDLLDSLNHSLAASELTAVDEAGFRRFVGHGGRVMIERAHAAQQRSLDVAEHDRLLKLFLDH
YTDNIPGKSRPYPGVIEAIARFEKAGYLLAICTNKYEANSLALIEALGLTRHFAAIAGQDTFAFRKPDPRHLTETIRLAG
GDAHRALMVGDSQTDIDTAKAAGIPVVAVDFGYTDRHVREFEPSAVISHFDALTVELAERLIRAAGH

Sequences:

>Translated_227_residues
MSRPIIVFDLDGTLIDTAPDLLDSLNHSLAASELTAVDEAGFRRFVGHGGRVMIERAHAAQQRSLDVAEHDRLLKLFLDH
YTDNIPGKSRPYPGVIEAIARFEKAGYLLAICTNKYEANSLALIEALGLTRHFAAIAGQDTFAFRKPDPRHLTETIRLAG
GDAHRALMVGDSQTDIDTAKAAGIPVVAVDFGYTDRHVREFEPSAVISHFDALTVELAERLIRAAGH
>Mature_226_residues
SRPIIVFDLDGTLIDTAPDLLDSLNHSLAASELTAVDEAGFRRFVGHGGRVMIERAHAAQQRSLDVAEHDRLLKLFLDHY
TDNIPGKSRPYPGVIEAIARFEKAGYLLAICTNKYEANSLALIEALGLTRHFAAIAGQDTFAFRKPDPRHLTETIRLAGG
DAHRALMVGDSQTDIDTAKAAGIPVVAVDFGYTDRHVREFEPSAVISHFDALTVELAERLIRAAGH

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family

Homologues:

Organism=Escherichia coli, GI1789787, Length=206, Percent_Identity=31.5533980582524, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GPH_RHILO (Q98ML8)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102309.1
- ProteinModelPortal:   Q98ML8
- SMR:   Q98ML8
- GeneID:   1224972
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr0528
- NMPDR:   fig|266835.1.peg.414
- HOGENOM:   HBG742904
- OMA:   HHFDELP
- ProtClustDB:   PRK13222
- BRENDA:   3.1.3.18
- HAMAP:   MF_00495
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198
- Gene3D:   G3DSA:3.40.50.1000
- Gene3D:   G3DSA:1.10.150.240
- TIGRFAMs:   TIGR01549
- TIGRFAMs:   TIGR01509
- TIGRFAMs:   TIGR01449

Pfam domain/function: PF00702 Hydrolase; SSF56784 SSF56784

EC number: =3.1.3.18

Molecular weight: Translated: 24821; Mature: 24690

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: ACT_SITE 9-9

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPIIVFDLDGTLIDTAPDLLDSLNHSLAASELTAVDEAGFRRFVGHGGRVMIERAHAA
CCCCEEEEECCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHH
QQRSLDVAEHDRLLKLFLDHYTDNIPGKSRPYPGVIEAIARFEKAGYLLAICTNKYEANS
HHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCH
LALIEALGLTRHFAAIAGQDTFAFRKPDPRHLTETIRLAGGDAHRALMVGDSQTDIDTAK
HHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHH
AAGIPVVAVDFGYTDRHVREFEPSAVISHFDALTVELAERLIRAAGH
HCCCCEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRPIIVFDLDGTLIDTAPDLLDSLNHSLAASELTAVDEAGFRRFVGHGGRVMIERAHAA
CCCEEEEECCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHH
QQRSLDVAEHDRLLKLFLDHYTDNIPGKSRPYPGVIEAIARFEKAGYLLAICTNKYEANS
HHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCH
LALIEALGLTRHFAAIAGQDTFAFRKPDPRHLTETIRLAGGDAHRALMVGDSQTDIDTAK
HHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHH
AAGIPVVAVDFGYTDRHVREFEPSAVISHFDALTVELAERLIRAAGH
HCCCCEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968