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The map label for this gene is kdsA [H]

Identifier: 13470612

GI number: 13470612

Start: 265492

End: 266352

Strand: Direct

Name: kdsA [H]

Synonym: mlr0374

Alternate gene names: 13470612

Gene position: 265492-266352 (Clockwise)

Preceding gene: 13470611

Following gene: 13470613

Centisome position: 3.77

GC content: 64.46

Gene sequence:

>861_bases
ATGAACAAGCCCCAAGTGCCCAATTCGATGGCACCCAATTCGTCGGTGACGGTCGGCAATGTCGTGTTCGACAACAATGC
GGCCCTGGCGCTGATCGCCGGCCCGTGCCAGTTCGAGTCGCGCCAGCATGCCTTCGACATGGCCGGCGCGCTGAAGGAAC
TGACGGCCAGGCTCGGCATCGGCCTCGTCTACAAGACCAGCTACGACAAGGCCAACCGCACCTCGCTGTCGGCAACGCGC
GGCGCCGGCATGGACGCCGCACTTCCGGTCTTCGACGAGTTGCGCAAGGAATTTTCCCTCCCCGTGCTCACCGATGTCCA
CACCGAGGAACAATGCGCGATCGTCGCGCCCCATGTCGATGTGCTGCAGATCCCAGCCTTCCTGTCACGCCAGACCGACA
TGTTGGTCGCCGCAGCGAAAACGGGCAAGGTGATCAACGTCAAGAAGGGGCAGTTCCTCGCCCCTTGGGACATGAAGAAC
GTGGTCGCCAAGATCACCGGGTCCGGCAACCCAAATGTGCTCACCACCGAGCGCGGCGCGTCCTTCGGCTACAACACGCT
GGTGTCTGACATGCGCGCGCTGCCTGTCATGGCCGAGATCGGCGCGCCGGTGATCTTCGACGCCACGCATTCGGTCCAGC
AGCCGGGCGGGCAGGGCGGCTCGTCCGGTGGCGAGCGCCGCTTTGTCGAGACGCTGGCCCGTGCGGCCGTCGCCGTCGGT
GTCGCCGGCGTCTTCATCGAGACCCACCAGGATCCCGACAATTCGACTTCTTCCGATGGGCCCAACATGCTGCCGCTGAA
GGATATGCCGGCTCTCCTGGAAAGGCTGATGGCTTTCGACCGGATCGCGAAGGGCCGCTGA

Upstream 100 bases:

>100_bases
TGCTTGCAGCCAATGCTATACAGCACGATATAGCGGGCACTGATATTGTCGTGCGGCCGGCATCCGGACAGGGCGCGGCT
TTCATCTTCCGGGAGATCCC

Downstream 100 bases:

>100_bases
ACCAGCGATTCCTGCCGCTGCTTGTGGTCAAGAGGGGCCAGTTGTACGCTTGGCCGGCAAATGAGCGTTTTGGCAGGAGG
AAGCCATGTCCGTCGCCCGC

Product: 2-dehydro-3-deoxyphosphooctonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase; KDO-8-phosphate synthase; KDO 8-P synthase; KDOPS; Phospho-2-dehydro-3-deoxyoctonate aldolase [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MNKPQVPNSMAPNSSVTVGNVVFDNNAALALIAGPCQFESRQHAFDMAGALKELTARLGIGLVYKTSYDKANRTSLSATR
GAGMDAALPVFDELRKEFSLPVLTDVHTEEQCAIVAPHVDVLQIPAFLSRQTDMLVAAAKTGKVINVKKGQFLAPWDMKN
VVAKITGSGNPNVLTTERGASFGYNTLVSDMRALPVMAEIGAPVIFDATHSVQQPGGQGGSSGGERRFVETLARAAVAVG
VAGVFIETHQDPDNSTSSDGPNMLPLKDMPALLERLMAFDRIAKGR

Sequences:

>Translated_286_residues
MNKPQVPNSMAPNSSVTVGNVVFDNNAALALIAGPCQFESRQHAFDMAGALKELTARLGIGLVYKTSYDKANRTSLSATR
GAGMDAALPVFDELRKEFSLPVLTDVHTEEQCAIVAPHVDVLQIPAFLSRQTDMLVAAAKTGKVINVKKGQFLAPWDMKN
VVAKITGSGNPNVLTTERGASFGYNTLVSDMRALPVMAEIGAPVIFDATHSVQQPGGQGGSSGGERRFVETLARAAVAVG
VAGVFIETHQDPDNSTSSDGPNMLPLKDMPALLERLMAFDRIAKGR
>Mature_286_residues
MNKPQVPNSMAPNSSVTVGNVVFDNNAALALIAGPCQFESRQHAFDMAGALKELTARLGIGLVYKTSYDKANRTSLSATR
GAGMDAALPVFDELRKEFSLPVLTDVHTEEQCAIVAPHVDVLQIPAFLSRQTDMLVAAAKTGKVINVKKGQFLAPWDMKN
VVAKITGSGNPNVLTTERGASFGYNTLVSDMRALPVMAEIGAPVIFDATHSVQQPGGQGGSSGGERRFVETLARAAVAVG
VAGVFIETHQDPDNSTSSDGPNMLPLKDMPALLERLMAFDRIAKGR

Specific function: Synthesis Of Kdo 8-P Which Is Required For Lipid A Maturation And Cellular Growth. [C]

COG id: COG2877

COG function: function code M; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsA family [H]

Homologues:

Organism=Escherichia coli, GI1787466, Length=272, Percent_Identity=44.1176470588235, Blast_Score=232, Evalue=2e-62,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006269 [H]

Pfam domain/function: PF00793 DAHP_synth_1 [H]

EC number: =2.5.1.55 [H]

Molecular weight: Translated: 30364; Mature: 30364

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKPQVPNSMAPNSSVTVGNVVFDNNAALALIAGPCQFESRQHAFDMAGALKELTARLGI
CCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
GLVYKTSYDKANRTSLSATRGAGMDAALPVFDELRKEFSLPVLTDVHTEEQCAIVAPHVD
CEEEECCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHCCCCEEECCCCCCCEEEECCCCH
VLQIPAFLSRQTDMLVAAAKTGKVINVKKGQFLAPWDMKNVVAKITGSGNPNVLTTERGA
HHHHHHHHCCCCCEEEEEECCCCEEEECCCCEECCCCHHHHHEEEECCCCCCEEEECCCC
SFGYNTLVSDMRALPVMAEIGAPVIFDATHSVQQPGGQGGSSGGERRFVETLARAAVAVG
CCCHHHHHHHHHHCCHHHHCCCCEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VAGVFIETHQDPDNSTSSDGPNMLPLKDMPALLERLMAFDRIAKGR
HHEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKPQVPNSMAPNSSVTVGNVVFDNNAALALIAGPCQFESRQHAFDMAGALKELTARLGI
CCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
GLVYKTSYDKANRTSLSATRGAGMDAALPVFDELRKEFSLPVLTDVHTEEQCAIVAPHVD
CEEEECCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHCCCCEEECCCCCCCEEEECCCCH
VLQIPAFLSRQTDMLVAAAKTGKVINVKKGQFLAPWDMKNVVAKITGSGNPNVLTTERGA
HHHHHHHHCCCCCEEEEEECCCCEEEECCCCEECCCCHHHHHEEEECCCCCCEEEECCCC
SFGYNTLVSDMRALPVMAEIGAPVIFDATHSVQQPGGQGGSSGGERRFVETLARAAVAVG
CCCHHHHHHHHHHCCHHHHCCCCEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VAGVFIETHQDPDNSTSSDGPNMLPLKDMPALLERLMAFDRIAKGR
HHEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]