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The map label for this gene is rsmE [H]

Identifier: 15603733

GI number: 15603733

Start: 2110367

End: 2111101

Strand: Direct

Name: rsmE [H]

Synonym: PM1868

Alternate gene names: 15603733

Gene position: 2110367-2111101 (Clockwise)

Preceding gene: 15603728

Following gene: 161723304

Centisome position: 93.48

GC content: 43.27

Gene sequence:

>735_bases
ATGAGAATACCTCGAATTTACCACCCTGTCCTATTACAAGGCGTGAAACATTGTGAATTAAGTGAAGATGCGGCTAACCA
TGTTGGACGTGTGTTACGTATGCAAGCCGGGGATAAACTCGTGCTTTTCGATGGCTCAAACCATGTGTATGACGCACAGA
TTACGCAGATCAGCAAAAAACATGTTGAAGTAGATATTTTGCAACACCAATGGGATGATCGAGAAAGTCCATTAAAAATT
CACCTTGGGCAAGTGATTTCACGAGGAGAAAGAATGGAATTCACCATTCAGAAATCCGTTGAATTGGGAGTACATGTGAT
TACACCTCTTTGGTCAGAGCGTTGTGGGGTGAAGTTAGATGCCGATCGTCTGGAGAAAAAAATCCAGCAATGGCAGAAAA
TTGCCGTTGCCGCCTGTGAACAATGTGGGCGTAATGTGGTGCCAGAAATACGTCCGATGATGAAATTACAAGATTGGTGT
GCGGAAAAAGACGGGGCATTGAAGTTGAATTTACATCCAAGGGCCCAATATTCAATACGTACCCTACCTGACATCCCAGC
AGCAGGGGTACGTTTATTAATTGGTTCTGAAGGGGGCTTATCCGCACAAGAGATTGCCCAAACAGAACAACAAGATTTTA
TTGAAGTATTATTAGGAAAGCGTGTATTGCGTACGGAAACAGCTTCGTTAGCTGCAATTACAGCATTGCAGATCTGTTTC
GGTGATTTAGGTTGA

Upstream 100 bases:

>100_bases
TGAAAACAAAATAGCCCATACTTTTGTTTAACCAAGCGCTTGCCTTTCTGTTATGATAGGAATATGACAAATCGCGGAAG
ATAAAATAATCATTGCAGTT

Downstream 100 bases:

>100_bases
GGAAGAGATATGATGGAATTACAAGATCATTTTTTAATTGCGATGCCTCAAATGGAAGATGATTATTTTGCCCATTCGGT
GGTTTATATTTGTGAGCACA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MRIPRIYHPVLLQGVKHCELSEDAANHVGRVLRMQAGDKLVLFDGSNHVYDAQITQISKKHVEVDILQHQWDDRESPLKI
HLGQVISRGERMEFTIQKSVELGVHVITPLWSERCGVKLDADRLEKKIQQWQKIAVAACEQCGRNVVPEIRPMMKLQDWC
AEKDGALKLNLHPRAQYSIRTLPDIPAAGVRLLIGSEGGLSAQEIAQTEQQDFIEVLLGKRVLRTETASLAAITALQICF
GDLG

Sequences:

>Translated_244_residues
MRIPRIYHPVLLQGVKHCELSEDAANHVGRVLRMQAGDKLVLFDGSNHVYDAQITQISKKHVEVDILQHQWDDRESPLKI
HLGQVISRGERMEFTIQKSVELGVHVITPLWSERCGVKLDADRLEKKIQQWQKIAVAACEQCGRNVVPEIRPMMKLQDWC
AEKDGALKLNLHPRAQYSIRTLPDIPAAGVRLLIGSEGGLSAQEIAQTEQQDFIEVLLGKRVLRTETASLAAITALQICF
GDLG
>Mature_244_residues
MRIPRIYHPVLLQGVKHCELSEDAANHVGRVLRMQAGDKLVLFDGSNHVYDAQITQISKKHVEVDILQHQWDDRESPLKI
HLGQVISRGERMEFTIQKSVELGVHVITPLWSERCGVKLDADRLEKKIQQWQKIAVAACEQCGRNVVPEIRPMMKLQDWC
AEKDGALKLNLHPRAQYSIRTLPDIPAAGVRLLIGSEGGLSAQEIAQTEQQDFIEVLLGKRVLRTETASLAAITALQICF
GDLG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=244, Percent_Identity=71.3114754098361, Blast_Score=353, Evalue=7e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27391; Mature: 27391

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPRIYHPVLLQGVKHCELSEDAANHVGRVLRMQAGDKLVLFDGSNHVYDAQITQISKK
CCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCEEEEHHHHHHHHH
HVEVDILQHQWDDRESPLKIHLGQVISRGERMEFTIQKSVELGVHVITPLWSERCGVKLD
HHHHHHHHHCCCCCCCCCEEEHHHHHCCCCCEEEEEEHHHHCCHHEECCHHHHHCCCEEC
ADRLEKKIQQWQKIAVAACEQCGRNVVPEIRPMMKLQDWCAEKDGALKLNLHPRAQYSIR
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEC
TLPDIPAAGVRLLIGSEGGLSAQEIAQTEQQDFIEVLLGKRVLRTETASLAAITALQICF
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
GDLG
HCCC
>Mature Secondary Structure
MRIPRIYHPVLLQGVKHCELSEDAANHVGRVLRMQAGDKLVLFDGSNHVYDAQITQISKK
CCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCEEEEHHHHHHHHH
HVEVDILQHQWDDRESPLKIHLGQVISRGERMEFTIQKSVELGVHVITPLWSERCGVKLD
HHHHHHHHHCCCCCCCCCEEEHHHHHCCCCCEEEEEEHHHHCCHHEECCHHHHHCCCEEC
ADRLEKKIQQWQKIAVAACEQCGRNVVPEIRPMMKLQDWCAEKDGALKLNLHPRAQYSIR
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEC
TLPDIPAAGVRLLIGSEGGLSAQEIAQTEQQDFIEVLLGKRVLRTETASLAAITALQICF
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
GDLG
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]