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The map label for this gene is queA

Identifier: 15602097

GI number: 15602097

Start: 259459

End: 260550

Strand: Reverse

Name: queA

Synonym: PM0232

Alternate gene names: 15602097

Gene position: 260550-259459 (Counterclockwise)

Preceding gene: 15602098

Following gene: 15602096

Centisome position: 11.54

GC content: 44.96

Gene sequence:

>1092_bases
ATGCGTGTTTCTGACTTTTATTTTGATTTACCCGATGAGCTGATTGCTCGTTACCCAAAACCGGATCGCACCGCTAGTCG
CTTATTACAACTTAACGGCGAAAATGGTGAGATTACCCACCGCACTTTTGCTGATATCCTTGATCAAATTCATGAAGGCG
ACTTATTAATTTTTAATAACACGCGCGTGATTCCTGCAAGGATGTTTGGACGTAAAGCCAGTGGCGGTAAGGTTGAAGTG
TTGGTTGAGCGGATTTTATCCGACACACGCTTTTTAGCCCATGTGCGCTCCTCAAAAGCACCGAAAGAAGGGGCAGAACT
GTGGTTAGGCGAAGATAAGTTAGGTGAACATCATGGGGTTAAAATGATCATGGTTGCGCGCCATGATACCTTATTTGAAT
TAGAAATCGCGCAAAAACAGACCGCACTTTTAGACGTGTTACAGCAAATTGGGCATATGCCATTGCCCCCTTATATTGAT
CGCCCTGACGAAGAAGCGGATAAAGAACGTTATCAAACCGTTTATAACAAAGTCCCTGGCGCGGTTGCGGCACCAACTGC
CGGCTTGCATTTTGACGATGCTTTATTAGCACAACTCAAAGCCAAAGGGGTTAACTTTGCTTTTGTGACTTTACATGTTG
GCGCAGGCACATTTCAACCGGTGCGCGTCGAGCAGATTGAAGATCACAAAATGCACGCAGAATATGTGGAAGTACCACAA
GAAGTGTGTGACGCGATTATTGCCACGAAACAAGCAGGCAAACGTGTGATTGCTGTGGGCACCACTTCAGTTCGTTCCGT
CGAAAGTGCGGCATTAGCGACGGAAGAAAAAGGCGAACATGCCTTAATTACGCCTTATTTTTCCGATACGTCAATTTTTA
TTTATCCAGGCAAAACTTTCCGCGTGGTGGATTGTTTAATTACGAACTTCCACTTACCAGAAAGTACACTGATTATGCTG
GTTTCCGCTTTTGCTGGCTATCGCCATACTATGCAGGCGTACCAAAGTGCGGTCGAAAATCGCTATCGTTTTTTTAGTTA
TGGCGACGCGATGTTTATCAGCAAAAATCCTCACGTTAATGGGCTTGACTGA

Upstream 100 bases:

>100_bases
GATACTAAACAACAAAATCTGGATTTTTCCTTGTGCAAGAGCGGAATTCACACTAGACTTACGCCCACGATTTTTTAGAT
AACTTTTTATAACCCTATTT

Downstream 100 bases:

>100_bases
TGATCTTTGTGTTTGATTTAGACGGCACGATTTGCTTTGACGGCAAACAGATTCCACTCAACATTCAACACGCTCTTGAA
CAGCTGATGCAAAAAGATCA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MRVSDFYFDLPDELIARYPKPDRTASRLLQLNGENGEITHRTFADILDQIHEGDLLIFNNTRVIPARMFGRKASGGKVEV
LVERILSDTRFLAHVRSSKAPKEGAELWLGEDKLGEHHGVKMIMVARHDTLFELEIAQKQTALLDVLQQIGHMPLPPYID
RPDEEADKERYQTVYNKVPGAVAAPTAGLHFDDALLAQLKAKGVNFAFVTLHVGAGTFQPVRVEQIEDHKMHAEYVEVPQ
EVCDAIIATKQAGKRVIAVGTTSVRSVESAALATEEKGEHALITPYFSDTSIFIYPGKTFRVVDCLITNFHLPESTLIML
VSAFAGYRHTMQAYQSAVENRYRFFSYGDAMFISKNPHVNGLD

Sequences:

>Translated_363_residues
MRVSDFYFDLPDELIARYPKPDRTASRLLQLNGENGEITHRTFADILDQIHEGDLLIFNNTRVIPARMFGRKASGGKVEV
LVERILSDTRFLAHVRSSKAPKEGAELWLGEDKLGEHHGVKMIMVARHDTLFELEIAQKQTALLDVLQQIGHMPLPPYID
RPDEEADKERYQTVYNKVPGAVAAPTAGLHFDDALLAQLKAKGVNFAFVTLHVGAGTFQPVRVEQIEDHKMHAEYVEVPQ
EVCDAIIATKQAGKRVIAVGTTSVRSVESAALATEEKGEHALITPYFSDTSIFIYPGKTFRVVDCLITNFHLPESTLIML
VSAFAGYRHTMQAYQSAVENRYRFFSYGDAMFISKNPHVNGLD
>Mature_363_residues
MRVSDFYFDLPDELIARYPKPDRTASRLLQLNGENGEITHRTFADILDQIHEGDLLIFNNTRVIPARMFGRKASGGKVEV
LVERILSDTRFLAHVRSSKAPKEGAELWLGEDKLGEHHGVKMIMVARHDTLFELEIAQKQTALLDVLQQIGHMPLPPYID
RPDEEADKERYQTVYNKVPGAVAAPTAGLHFDDALLAQLKAKGVNFAFVTLHVGAGTFQPVRVEQIEDHKMHAEYVEVPQ
EVCDAIIATKQAGKRVIAVGTTSVRSVESAALATEEKGEHALITPYFSDTSIFIYPGKTFRVVDCLITNFHLPESTLIML
VSAFAGYRHTMQAYQSAVENRYRFFSYGDAMFISKNPHVNGLD

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=358, Percent_Identity=67.3184357541899, Blast_Score=503, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_PASMU (P57832)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245169.1
- ProteinModelPortal:   P57832
- SMR:   P57832
- GeneID:   1243579
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0232
- NMPDR:   fig|272843.1.peg.232
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- BioCyc:   PMUL272843:PM0232-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 40654; Mature: 40654

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSDFYFDLPDELIARYPKPDRTASRLLQLNGENGEITHRTFADILDQIHEGDLLIFNN
CCCCCEEECCCHHHHHCCCCCCHHHHHHEEECCCCCCCHHHHHHHHHHHHCCCCEEEECC
TRVIPARMFGRKASGGKVEVLVERILSDTRFLAHVRSSKAPKEGAELWLGEDKLGEHHGV
CEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEECCHHCCCCCCE
KMIMVARHDTLFELEIAQKQTALLDVLQQIGHMPLPPYIDRPDEEADKERYQTVYNKVPG
EEEEEECCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
AVAAPTAGLHFDDALLAQLKAKGVNFAFVTLHVGAGTFQPVRVEQIEDHKMHAEYVEVPQ
CCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHH
EVCDAIIATKQAGKRVIAVGTTSVRSVESAALATEEKGEHALITPYFSDTSIFIYPGKTF
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEECCCCH
RVVDCLITNFHLPESTLIMLVSAFAGYRHTMQAYQSAVENRYRFFSYGDAMFISKNPHVN
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCEEEEECCCCCC
GLD
CCC
>Mature Secondary Structure
MRVSDFYFDLPDELIARYPKPDRTASRLLQLNGENGEITHRTFADILDQIHEGDLLIFNN
CCCCCEEECCCHHHHHCCCCCCHHHHHHEEECCCCCCCHHHHHHHHHHHHCCCCEEEECC
TRVIPARMFGRKASGGKVEVLVERILSDTRFLAHVRSSKAPKEGAELWLGEDKLGEHHGV
CEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEECCHHCCCCCCE
KMIMVARHDTLFELEIAQKQTALLDVLQQIGHMPLPPYIDRPDEEADKERYQTVYNKVPG
EEEEEECCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
AVAAPTAGLHFDDALLAQLKAKGVNFAFVTLHVGAGTFQPVRVEQIEDHKMHAEYVEVPQ
CCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHH
EVCDAIIATKQAGKRVIAVGTTSVRSVESAALATEEKGEHALITPYFSDTSIFIYPGKTF
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEECCCCH
RVVDCLITNFHLPESTLIMLVSAFAGYRHTMQAYQSAVENRYRFFSYGDAMFISKNPHVN
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCEEEEECCCCCC
GLD
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100