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The map label for this gene is acnB [H]

Identifier: 15602069

GI number: 15602069

Start: 227888

End: 230494

Strand: Reverse

Name: acnB [H]

Synonym: PM0204

Alternate gene names: 15602069

Gene position: 230494-227888 (Counterclockwise)

Preceding gene: 15602075

Following gene: 15602068

Centisome position: 10.21

GC content: 43.96

Gene sequence:

>2607_bases
ATGTCTGACTTTCTACAATCTTATCAACAACATGTTGATGAACGTGCGGCATTAGGCATTGTGCCGAAACCTTTAGATGC
AGAACAAACCGCCCAATTAATTGAACTCCTAAAAAACCCACCTGCTGATAAAGCTGACTTTCTTTTAAATTTATTCGAAA
CGCGTATCCCTGCTGGTGTTGATGAAGCCGCCTATGTGAAAGCCTCGTTTCTCGCCGCCGTCGCAAAACGTGATGTACAG
TCTCTACTCATTTCCGCAGAAAAAGCCGTGCAACTATTAGGAACGATGCAAGGTGGCTATAATATTGAGCCGCTTCTTCA
TGCGCTTGATGATGAAACACTCGCCCCAATTGCGGTTGAAGCACTTTCTACCACGCTTTTAATGTTCGATAACTTCCACG
ATGTAGCTGAGCGAGCGAAGAAAGGCAATCTATTTGCCAAACAAGTTTTGACTTCGTGGGCAAATGCAGATTGGTTTTTA
TCTCGTCCAAAATTAGCGGAAAAACTCACTGTTACCGTATTTAAAGTAACAGGGGAAACCAATACCGACGATCTGTCACC
TGCCCAAGATGCCTGGTCACGTCCAGATATTCCATTACATGCATTGGCGATGTTAAAAAATACCCGTGATGGCATTGAGC
CTGACGATGCTGGCAATGTCGGACCAATTAAACAATTAGAACAATTAAAACAAAAAGGTTTTCCATTGGCTTATGTCGGC
GATGTTGTCGGGACAGGCTCTTCACGTAAATCCGCCACCAACTCTGTGCTTTGGTTTATGGGGGAAGATATTCCTTATAT
TCCAAATAAACGTGCTGGCGGTGTGGTACTGGGTGGCAAAATTGCGCCGATTTTCTTCAACACAATGGAAGACGCCGGTT
CATTACCAATTGAAGTGGATGTCAGTGAACTCAACATGGGGGATGTGATTGATATTTATCCATACCAAGGCAAAATTTGT
CGTCATGATACTGACGAGGTTTTAGCTGAATTTAGTTTAAAAACCAATGTGCTGTTAGATGAAGTGCGTGCAGGTGGTCG
TATTCCATTAATCATTGGACGTGGTCTAACCCATAAAGCACGTTGTGAATTAGGTCTGCCTGAAAGTGATATTTTTGCCA
AACCACAATGCAGTACAGAAAGCCAAAAAGGTTTTACCCTTGCCCAAAAAATGGTGGGGCGCGCTTGTGGTGTGGACGGT
ATTCGTCCAGGACAATACTGTGAGCCACGCATGACCTCTGTCGGTTCACAAGATACCACTGGACCAATGACTCGTGATGA
GTTAAAAGACCTTGCTTGTTTGGGTTTCTCCGCTGACTTAGTGATGCAATCTTTCTGTCATACGGCGGCTTATCCAAAAC
CGGTTGACGTAGTCACACATCACACACTCCCTGATTTCATTATGAATCGTGGCGGGGTTTCATTACGTCCTGGTGATGGT
GTCATTCACTCTTGGCTAAACCGTATGTTATTACCTGATACAGTTGGTACAGGTGGTGACTCGCATACGCGTTTCCCGAT
TGGAATTTCCTTCCCTGCGGGTTCTGGCTTAGTGGCATTTGCAGCGGCCACAGGTGTCATGCCATTAGATATGCCGGAGT
CAGTATTAGTGCGCTTTAAAGGCGAAATGCAACCGGGAATTACCTTGCGCGATTTGGTGCATGCGATCCCTTATTATGCG
ATTAAACAAGGTTTGCTCACCGTAGAGAAAAAAGGCAAGAAAAACATTTTCTCTGGACGTATTTTAGAAATTGAAGGCTT
AGAGAACTTAAAAGTCGAACAGGCGTTTGAGCTTTCTGATGCATCAGCAGAACGTTCTGCCGCAGCTTGTACAATTAAAC
TGGCGAAAGAACCCATTATTGAATATTTAAATTCAAATATCGTGTTATTGAAATATATGATTGCCGAAGGCTATGGCGAT
GCGCGCACACTTGAGCGCCGGATTAAAGGCATGGAGCAATGGCTGGCTGATCCACAACTAATGGAAGCCGATCCTGATGC
TGAATATGCGGCAGTGATTGAAATTGACCTCGCTGAAATCAAAGAACCGATTTTATGTGCACCAAATGATCCCGATGACG
CGCGTTTATTGTCCGAAGTCCAAGGCGATAAAATTGATGAAGTCTTTATCGGATCTTGCATGACAAATATAGGTCACTTC
CGTGCAGCAGGAAAATTATTAGCCAAGTTCAAAGGCATGATTCCAACTCGCCTCTGGATTGCCCCACCAACCAAAATGGA
TGCGGCATTATTAAGTGAAGAAGGTTATTACAGTATTTATGGTAAAAGTGGCGCACGGATTGAGATTCCAGGTTGTTCAT
TATGCATGGGTAACCAAGCGCGTGTTGCTGATAATGCCACGGTGGTTTCTACTTCAACGCGTAACTTCCCAAACCGTTTA
GGACAAGGTGCAAATGTTTATCTTGCATCTGCCGAGCTAGCCGCGGTTTCGGCGATTTTAGGTAAATTACCAACGTCAGA
AGAATACCTCACCTTTGCAGCAGACTTACAACAAGACAAGGACGACACTTATCGTTATATGAACTTTGACCAAATCAACA
GCTATACTAAAAAAGCAGATAACGTGATTTTTCAAAGTGCGGTATAA

Upstream 100 bases:

>100_bases
TTATAGTATGTTTTAACAATTTCGTAACGAAACCTTACCGCACTTGATACGAATGCGGTTTTCCCTAACAGAAATAAAAG
CAAACCCCATGAGGTAACCT

Downstream 100 bases:

>100_bases
TTTTCAAGAGTTTTAACACCACAAACGGGGAACATCAGTTCCCCGTTCGCTATGAATGATCACCGTTTTCTCTGACGAGA
AAATAAAAGGAATTAAACAT

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase; 2-methylisocitrate dehydratase; Citrate hydro-lyase [H]

Number of amino acids: Translated: 868; Mature: 867

Protein sequence:

>868_residues
MSDFLQSYQQHVDERAALGIVPKPLDAEQTAQLIELLKNPPADKADFLLNLFETRIPAGVDEAAYVKASFLAAVAKRDVQ
SLLISAEKAVQLLGTMQGGYNIEPLLHALDDETLAPIAVEALSTTLLMFDNFHDVAERAKKGNLFAKQVLTSWANADWFL
SRPKLAEKLTVTVFKVTGETNTDDLSPAQDAWSRPDIPLHALAMLKNTRDGIEPDDAGNVGPIKQLEQLKQKGFPLAYVG
DVVGTGSSRKSATNSVLWFMGEDIPYIPNKRAGGVVLGGKIAPIFFNTMEDAGSLPIEVDVSELNMGDVIDIYPYQGKIC
RHDTDEVLAEFSLKTNVLLDEVRAGGRIPLIIGRGLTHKARCELGLPESDIFAKPQCSTESQKGFTLAQKMVGRACGVDG
IRPGQYCEPRMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVVTHHTLPDFIMNRGGVSLRPGDG
VIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGEMQPGITLRDLVHAIPYYA
IKQGLLTVEKKGKKNIFSGRILEIEGLENLKVEQAFELSDASAERSAAACTIKLAKEPIIEYLNSNIVLLKYMIAEGYGD
ARTLERRIKGMEQWLADPQLMEADPDAEYAAVIEIDLAEIKEPILCAPNDPDDARLLSEVQGDKIDEVFIGSCMTNIGHF
RAAGKLLAKFKGMIPTRLWIAPPTKMDAALLSEEGYYSIYGKSGARIEIPGCSLCMGNQARVADNATVVSTSTRNFPNRL
GQGANVYLASAELAAVSAILGKLPTSEEYLTFAADLQQDKDDTYRYMNFDQINSYTKKADNVIFQSAV

Sequences:

>Translated_868_residues
MSDFLQSYQQHVDERAALGIVPKPLDAEQTAQLIELLKNPPADKADFLLNLFETRIPAGVDEAAYVKASFLAAVAKRDVQ
SLLISAEKAVQLLGTMQGGYNIEPLLHALDDETLAPIAVEALSTTLLMFDNFHDVAERAKKGNLFAKQVLTSWANADWFL
SRPKLAEKLTVTVFKVTGETNTDDLSPAQDAWSRPDIPLHALAMLKNTRDGIEPDDAGNVGPIKQLEQLKQKGFPLAYVG
DVVGTGSSRKSATNSVLWFMGEDIPYIPNKRAGGVVLGGKIAPIFFNTMEDAGSLPIEVDVSELNMGDVIDIYPYQGKIC
RHDTDEVLAEFSLKTNVLLDEVRAGGRIPLIIGRGLTHKARCELGLPESDIFAKPQCSTESQKGFTLAQKMVGRACGVDG
IRPGQYCEPRMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVVTHHTLPDFIMNRGGVSLRPGDG
VIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGEMQPGITLRDLVHAIPYYA
IKQGLLTVEKKGKKNIFSGRILEIEGLENLKVEQAFELSDASAERSAAACTIKLAKEPIIEYLNSNIVLLKYMIAEGYGD
ARTLERRIKGMEQWLADPQLMEADPDAEYAAVIEIDLAEIKEPILCAPNDPDDARLLSEVQGDKIDEVFIGSCMTNIGHF
RAAGKLLAKFKGMIPTRLWIAPPTKMDAALLSEEGYYSIYGKSGARIEIPGCSLCMGNQARVADNATVVSTSTRNFPNRL
GQGANVYLASAELAAVSAILGKLPTSEEYLTFAADLQQDKDDTYRYMNFDQINSYTKKADNVIFQSAV
>Mature_867_residues
SDFLQSYQQHVDERAALGIVPKPLDAEQTAQLIELLKNPPADKADFLLNLFETRIPAGVDEAAYVKASFLAAVAKRDVQS
LLISAEKAVQLLGTMQGGYNIEPLLHALDDETLAPIAVEALSTTLLMFDNFHDVAERAKKGNLFAKQVLTSWANADWFLS
RPKLAEKLTVTVFKVTGETNTDDLSPAQDAWSRPDIPLHALAMLKNTRDGIEPDDAGNVGPIKQLEQLKQKGFPLAYVGD
VVGTGSSRKSATNSVLWFMGEDIPYIPNKRAGGVVLGGKIAPIFFNTMEDAGSLPIEVDVSELNMGDVIDIYPYQGKICR
HDTDEVLAEFSLKTNVLLDEVRAGGRIPLIIGRGLTHKARCELGLPESDIFAKPQCSTESQKGFTLAQKMVGRACGVDGI
RPGQYCEPRMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVVTHHTLPDFIMNRGGVSLRPGDGV
IHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGEMQPGITLRDLVHAIPYYAI
KQGLLTVEKKGKKNIFSGRILEIEGLENLKVEQAFELSDASAERSAAACTIKLAKEPIIEYLNSNIVLLKYMIAEGYGDA
RTLERRIKGMEQWLADPQLMEADPDAEYAAVIEIDLAEIKEPILCAPNDPDDARLLSEVQGDKIDEVFIGSCMTNIGHFR
AAGKLLAKFKGMIPTRLWIAPPTKMDAALLSEEGYYSIYGKSGARIEIPGCSLCMGNQARVADNATVVSTSTRNFPNRLG
QGANVYLASAELAAVSAILGKLPTSEEYLTFAADLQQDKDDTYRYMNFDQINSYTKKADNVIFQSAV

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=865, Percent_Identity=81.6184971098266, Blast_Score=1433, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=378, Percent_Identity=26.1904761904762, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323335, Length=381, Percent_Identity=25.4593175853018, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 94518; Mature: 94387

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFLQSYQQHVDERAALGIVPKPLDAEQTAQLIELLKNPPADKADFLLNLFETRIPAGV
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
DEAAYVKASFLAAVAKRDVQSLLISAEKAVQLLGTMQGGYNIEPLLHALDDETLAPIAVE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHH
ALSTTLLMFDNFHDVAERAKKGNLFAKQVLTSWANADWFLSRPKLAEKLTVTVFKVTGET
HHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEECCCCHHCEEEEEEEEEECCC
NTDDLSPAQDAWSRPDIPLHALAMLKNTRDGIEPDDAGNVGPIKQLEQLKQKGFPLAYVG
CCCCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEC
DVVGTGSSRKSATNSVLWFMGEDIPYIPNKRAGGVVLGGKIAPIFFNTMEDAGSLPIEVD
HHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCEEEEEEEECHHHCCCCCEEEE
VSELNMGDVIDIYPYQGKICRHDTDEVLAEFSLKTNVLLDEVRAGGRIPLIIGRGLTHKA
HHHCCCCCEEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHH
RCELGLPESDIFAKPQCSTESQKGFTLAQKMVGRACGVDGIRPGQYCEPRMTSVGSQDTT
HCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCC
GPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVVTHHTLPDFIMNRGGVSLRPGDG
CCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHCCCCCEECCCCC
VIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFK
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHEEC
GEMQPGITLRDLVHAIPYYAIKQGLLTVEKKGKKNIFSGRILEIEGLENLKVEQAFELSD
CCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEECCCCCCCHHHHHCCCC
ASAERSAAACTIKLAKEPIIEYLNSNIVLLKYMIAEGYGDARTLERRIKGMEQWLADPQL
CCCHHCHHEEEEEHHHHHHHHHHCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCC
MEADPDAEYAAVIEIDLAEIKEPILCAPNDPDDARLLSEVQGDKIDEVFIGSCMTNIGHF
CCCCCCCCEEEEEEEEHHHCCCCEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RAAGKLLAKFKGMIPTRLWIAPPTKMDAALLSEEGYYSIYGKSGARIEIPGCSLCMGNQA
HHHHHHHHHHHCCCCCEEEECCCCCHHHHHHCCCCEEEEECCCCCEEECCCCCEEECCCC
RVADNATVVSTSTRNFPNRLGQGANVYLASAELAAVSAILGKLPTSEEYLTFAADLQQDK
EECCCCEEEECCCCCCHHHHCCCCEEEEECHHHHHHHHHHHCCCCCHHHEEEHHHHCCCC
DDTYRYMNFDQINSYTKKADNVIFQSAV
CCCEEECCHHHHHHHHHHHHCEEEECCC
>Mature Secondary Structure 
SDFLQSYQQHVDERAALGIVPKPLDAEQTAQLIELLKNPPADKADFLLNLFETRIPAGV
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
DEAAYVKASFLAAVAKRDVQSLLISAEKAVQLLGTMQGGYNIEPLLHALDDETLAPIAVE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHH
ALSTTLLMFDNFHDVAERAKKGNLFAKQVLTSWANADWFLSRPKLAEKLTVTVFKVTGET
HHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEECCCCHHCEEEEEEEEEECCC
NTDDLSPAQDAWSRPDIPLHALAMLKNTRDGIEPDDAGNVGPIKQLEQLKQKGFPLAYVG
CCCCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEC
DVVGTGSSRKSATNSVLWFMGEDIPYIPNKRAGGVVLGGKIAPIFFNTMEDAGSLPIEVD
HHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCEEEEEEEECHHHCCCCCEEEE
VSELNMGDVIDIYPYQGKICRHDTDEVLAEFSLKTNVLLDEVRAGGRIPLIIGRGLTHKA
HHHCCCCCEEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHH
RCELGLPESDIFAKPQCSTESQKGFTLAQKMVGRACGVDGIRPGQYCEPRMTSVGSQDTT
HCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCC
GPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVVTHHTLPDFIMNRGGVSLRPGDG
CCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHCCCCCEECCCCC
VIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFK
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHEEC
GEMQPGITLRDLVHAIPYYAIKQGLLTVEKKGKKNIFSGRILEIEGLENLKVEQAFELSD
CCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEECCCCCCCHHHHHCCCC
ASAERSAAACTIKLAKEPIIEYLNSNIVLLKYMIAEGYGDARTLERRIKGMEQWLADPQL
CCCHHCHHEEEEEHHHHHHHHHHCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCC
MEADPDAEYAAVIEIDLAEIKEPILCAPNDPDDARLLSEVQGDKIDEVFIGSCMTNIGHF
CCCCCCCCEEEEEEEEHHHCCCCEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RAAGKLLAKFKGMIPTRLWIAPPTKMDAALLSEEGYYSIYGKSGARIEIPGCSLCMGNQA
HHHHHHHHHHHCCCCCEEEECCCCCHHHHHHCCCCEEEEECCCCCEEECCCCCEEECCCC
RVADNATVVSTSTRNFPNRLGQGANVYLASAELAAVSAILGKLPTSEEYLTFAADLQQDK
EECCCCEEEECCCCCCHHHHCCCCEEEEECHHHHHHHHHHHCCCCCHHHEEEHHHHCCCC
DDTYRYMNFDQINSYTKKADNVIFQSAV
CCCEEECCHHHHHHHHHHHHCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 8932712; 9298646; 10585860; 8000525; 11992126 [H]