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The map label for this gene is prfB [H]

Identifier: 15602055

GI number: 15602055

Start: 212786

End: 213748

Strand: Reverse

Name: prfB [H]

Synonym: PM0190

Alternate gene names: 15602055

Gene position: 213748-212786 (Counterclockwise)

Preceding gene: 15602060

Following gene: 15602054

Centisome position: 9.47

GC content: 43.41

Gene sequence:

>963_bases
GTGTGGAACGAACCAGAAAAAGCACAAGCATTAGGTAAAGAACGGGTTGCCTTAGAAGGGGTCGTCAATACAATCCATAC
ACTTGATCAAGGCTTAGAAGATGTCGAAGGGCTACTCGAACTAGCAATTGAAGCCGAAGATGAAGCGACTTTCCATGAAG
CCGTCGCAGAGCTCGAAGAATTAGAACAACAGTTGGCAAAATTAGAATTTCGCCGTATGTTTAGTGGTCAACATGATGCA
GCAGACTGCTATGTTGACTTACAAGCGGGCTCTGGTGGTACCGAAGCACAAGATTGGACTGAAATGTTACTGCGTATGTA
TTTACGCTGGGCTGAAAGTAAAGGCTTTAAAACCGAATTAATGGAAGTGTCCGATGGGGATGTTGCCGGAATTAAATCCG
CCACGATTAAAGTTTCTGGTGAATATGCTTTTGGTTGGCTACGCACAGAAACCGGCATTCACCGTTTAGTACGTAAAAGT
CCATTTGACTCCAACAACCGTCGTCATACCTCGTTTAGTGCGGCATTCGTTTATCCTGAAATTGATGACGATATTGATAT
CGACATTAATCCTGCAGATCTGCGTATTGATGTTTATCGTGCTTCAGGTGCCGGTGGTCAGCACGTCAATAAAACCGAAA
GTGCGGTGCGTATTACCCATATTCCAAGTGGCATTGTGGTGCAATGCCAAAATGATCGCTCTCAACATAAAAACAAAGAT
CAATGTATGAAACAGTTGAAAGCGAAACTGTATGAAATGGAATTGCAAAAGAAGAATGCCGATAAACAAGCCATGGAAGA
CAACAAATCCGATATTGGTTGGGGCAGTCAAATCCGTTCCTATGTGTTAGACGATTCACGCATTAAAGATTTACGCACGG
GTGTGGAAAATCGCAATACCCAAGCAGTGCTCGATGGCGACTTAGATCGCTTTATTGAGGCCAGTTTAAAAGCCGGATTA
TAA

Upstream 100 bases:

>100_bases
GACCTCACCGAACGCACGCAAGTGTTGAGGGGGTATCTTTGACTTCGATGCGAAAGTTGAACGTTTAGAAGAAGTCAATG
CCGAACTCGAACAACCTGAT

Downstream 100 bases:

>100_bases
ATCCTCTGGGGGCTTCCCCCGACTAAAAAATACTAAAAATTAGGTAACCAAAATGTCAGAACAAACTCAAGAACTTGATC
TTAATGGCGAGATGTTAGTC

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MWNEPEKAQALGKERVALEGVVNTIHTLDQGLEDVEGLLELAIEAEDEATFHEAVAELEELEQQLAKLEFRRMFSGQHDA
ADCYVDLQAGSGGTEAQDWTEMLLRMYLRWAESKGFKTELMEVSDGDVAGIKSATIKVSGEYAFGWLRTETGIHRLVRKS
PFDSNNRRHTSFSAAFVYPEIDDDIDIDINPADLRIDVYRASGAGGQHVNKTESAVRITHIPSGIVVQCQNDRSQHKNKD
QCMKQLKAKLYEMELQKKNADKQAMEDNKSDIGWGSQIRSYVLDDSRIKDLRTGVENRNTQAVLDGDLDRFIEASLKAGL

Sequences:

>Translated_320_residues
MWNEPEKAQALGKERVALEGVVNTIHTLDQGLEDVEGLLELAIEAEDEATFHEAVAELEELEQQLAKLEFRRMFSGQHDA
ADCYVDLQAGSGGTEAQDWTEMLLRMYLRWAESKGFKTELMEVSDGDVAGIKSATIKVSGEYAFGWLRTETGIHRLVRKS
PFDSNNRRHTSFSAAFVYPEIDDDIDIDINPADLRIDVYRASGAGGQHVNKTESAVRITHIPSGIVVQCQNDRSQHKNKD
QCMKQLKAKLYEMELQKKNADKQAMEDNKSDIGWGSQIRSYVLDDSRIKDLRTGVENRNTQAVLDGDLDRFIEASLKAGL
>Mature_320_residues
MWNEPEKAQALGKERVALEGVVNTIHTLDQGLEDVEGLLELAIEAEDEATFHEAVAELEELEQQLAKLEFRRMFSGQHDA
ADCYVDLQAGSGGTEAQDWTEMLLRMYLRWAESKGFKTELMEVSDGDVAGIKSATIKVSGEYAFGWLRTETGIHRLVRKS
PFDSNNRRHTSFSAAFVYPEIDDDIDIDINPADLRIDVYRASGAGGQHVNKTESAVRITHIPSGIVVQCQNDRSQHKNKD
QCMKQLKAKLYEMELQKKNADKQAMEDNKSDIGWGSQIRSYVLDDSRIKDLRTGVENRNTQAVLDGDLDRFIEASLKAGL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=308, Percent_Identity=34.0909090909091, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI34577120, Length=322, Percent_Identity=32.6086956521739, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI166795305, Length=214, Percent_Identity=34.5794392523364, Blast_Score=115, Evalue=4e-26,
Organism=Escherichia coli, GI2367172, Length=320, Percent_Identity=82.1875, Blast_Score=567, Evalue=1e-163,
Organism=Escherichia coli, GI1787462, Length=242, Percent_Identity=40.495867768595, Blast_Score=189, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=238, Percent_Identity=34.0336134453782, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6321295, Length=324, Percent_Identity=33.641975308642, Blast_Score=155, Evalue=8e-39,
Organism=Drosophila melanogaster, GI19921226, Length=207, Percent_Identity=40.5797101449275, Blast_Score=157, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 35952; Mature: 35952

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWNEPEKAQALGKERVALEGVVNTIHTLDQGLEDVEGLLELAIEAEDEATFHEAVAELEE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LEQQLAKLEFRRMFSGQHDAADCYVDLQAGSGGTEAQDWTEMLLRMYLRWAESKGFKTEL
HHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
MEVSDGDVAGIKSATIKVSGEYAFGWLRTETGIHRLVRKSPFDSNNRRHTSFSAAFVYPE
EECCCCCCCCCCEEEEEEECCEEEEEEEHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCC
IDDDIDIDINPADLRIDVYRASGAGGQHVNKTESAVRITHIPSGIVVQCQNDRSQHKNKD
CCCCCEEEECCCCEEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCHH
QCMKQLKAKLYEMELQKKNADKQAMEDNKSDIGWGSQIRSYVLDDSRIKDLRTGVENRNT
HHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCC
QAVLDGDLDRFIEASLKAGL
EEEECCCHHHHHHHHHHCCC
>Mature Secondary Structure
MWNEPEKAQALGKERVALEGVVNTIHTLDQGLEDVEGLLELAIEAEDEATFHEAVAELEE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LEQQLAKLEFRRMFSGQHDAADCYVDLQAGSGGTEAQDWTEMLLRMYLRWAESKGFKTEL
HHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
MEVSDGDVAGIKSATIKVSGEYAFGWLRTETGIHRLVRKSPFDSNNRRHTSFSAAFVYPE
EECCCCCCCCCCEEEEEEECCEEEEEEEHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCC
IDDDIDIDINPADLRIDVYRASGAGGQHVNKTESAVRITHIPSGIVVQCQNDRSQHKNKD
CCCCCEEEECCCCEEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCHH
QCMKQLKAKLYEMELQKKNADKQAMEDNKSDIGWGSQIRSYVLDDSRIKDLRTGVENRNT
HHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCC
QAVLDGDLDRFIEASLKAGL
EEEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]