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The map label for this gene is ptsN [H]

Identifier: 15602035

GI number: 15602035

Start: 196120

End: 196638

Strand: Reverse

Name: ptsN [H]

Synonym: PM0170

Alternate gene names: 15602035

Gene position: 196638-196120 (Counterclockwise)

Preceding gene: 15602036

Following gene: 15602034

Centisome position: 8.71

GC content: 37.38

Gene sequence:

>519_bases
ATGATTAAATTTACAGAATTACTCAAACCTGAAAATATTCGTCAAGGGCTGATTTGCTCAAGTAAAAAACGTTTATTTGA
AATCATCACGCAAGTGATCGCAGATCAAATTCAATTAGAAAACGCAGAACAACTTTGTTTTGAGTGTTTACTAAGCCGTG
AAAAGATGGGGAACTCAGGCTTAGGCAACGGGATTGCTATGCCTAAAGCGAGAATCCCAGAAGGCAATCAACCACTGGCG
GTGTTTATTCATTTAGATTCACCTGTGGACTACGAGTCTGCCGATCATCGTGATGTGGATTTAATCTTGGCGATTTTAGT
ACCCGAACAACTGTGTGAACGCTATATCAGCGCATTACCAGAAATTGCCGAAAAATTTTCAGATAAAACCTTGTGTAAAC
AATTACGTAATGCACAAAATGTGGATGAAATCTGGCAAATTTTTGAAAATGTGGATCAATTGAATACACAGGAAAACGTA
ATAAAAGCCTCAGCAGAAACAACAAAAGAAACAGAATAA

Upstream 100 bases:

>100_bases
TGAGCGCGGGAAAAATGATTGCCACTGGCACACCTGAGCAAATTCTAAATAATGAACAAGTGAAACGTGTTTACTTAGGT
GAAGAATTTAAACTCTAGTT

Downstream 100 bases:

>100_bases
TCTGTTTCAGCTTAAAGGGGGAAAGCGTATGGAAATTATCATTATTAGTGGGCACTCTGGTGCGGGCAAGTCCGTTGCTC
TCCGTTGTTTAGAGGACATT

Product: hypothetical protein

Products: protein histidine; sugar phosphate

Alternate protein names: Putative phosphotransferase enzyme IIA component; Putative PTS system EIIA component [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MIKFTELLKPENIRQGLICSSKKRLFEIITQVIADQIQLENAEQLCFECLLSREKMGNSGLGNGIAMPKARIPEGNQPLA
VFIHLDSPVDYESADHRDVDLILAILVPEQLCERYISALPEIAEKFSDKTLCKQLRNAQNVDEIWQIFENVDQLNTQENV
IKASAETTKETE

Sequences:

>Translated_172_residues
MIKFTELLKPENIRQGLICSSKKRLFEIITQVIADQIQLENAEQLCFECLLSREKMGNSGLGNGIAMPKARIPEGNQPLA
VFIHLDSPVDYESADHRDVDLILAILVPEQLCERYISALPEIAEKFSDKTLCKQLRNAQNVDEIWQIFENVDQLNTQENV
IKASAETTKETE
>Mature_172_residues
MIKFTELLKPENIRQGLICSSKKRLFEIITQVIADQIQLENAEQLCFECLLSREKMGNSGLGNGIAMPKARIPEGNQPLA
VFIHLDSPVDYESADHRDVDLILAILVPEQLCERYISALPEIAEKFSDKTLCKQLRNAQNVDEIWQIFENVDQLNTQENV
IKASAETTKETE

Specific function: Not known; lacks the phosphorylation site found in other ptsN proteins [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=152, Percent_Identity=36.8421052631579, Blast_Score=121, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 19502; Mature: 19502

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKFTELLKPENIRQGLICSSKKRLFEIITQVIADQIQLENAEQLCFECLLSREKMGNSG
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
LGNGIAMPKARIPEGNQPLAVFIHLDSPVDYESADHRDVDLILAILVPEQLCERYISALP
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EIAEKFSDKTLCKQLRNAQNVDEIWQIFENVDQLNTQENVIKASAETTKETE
HHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIKFTELLKPENIRQGLICSSKKRLFEIITQVIADQIQLENAEQLCFECLLSREKMGNSG
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
LGNGIAMPKARIPEGNQPLAVFIHLDSPVDYESADHRDVDLILAILVPEQLCERYISALP
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EIAEKFSDKTLCKQLRNAQNVDEIWQIFENVDQLNTQENVIKASAETTKETE
HHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]