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The map label for this gene is pfs

Identifier: 30024058

GI number: 30024058

Start: 1949415

End: 1950095

Strand: Reverse

Name: pfs

Synonym: L132777

Alternate gene names: 30024058

Gene position: 1950095-1949415 (Counterclockwise)

Preceding gene: 15673863

Following gene: 15673861

Centisome position: 82.44

GC content: 35.68

Gene sequence:

>681_bases
ATGAAAATAGGAATTATTTGTGCGATGGATGAAGAAATTCGCATACTTGTTGAAAACTTAGAAAATGCAGAAAAACATAC
TCGTCATGGTTTAGTTTTTCATACAGGTTCAATTGGCCGGCATGAAGTGGTCCTTGTCCAATCTGGAATTGGTAAAGTGA
TGTCTGCTTTGGCAGTTGGTTTTTTGGTTGACATTTTTGATGTCGACGCCATTATCAATACTGGATCAGCGGGAGCAGTT
GCTCAAGGTTTAGCAATTGGTGATGTTGTAGTTGCTGATAAACTTGCTTATCATGATGTTGATGTTACTGCTTTTGGTTA
TGCTTATGGCCAAATGGCTCAACAACCTTTATATTTTGAATCAAGTAAATATTTTGTTTCTGAATTGAAAAAAGTTTTAG
AAAATGTGCATGTTGGATTGATTACTAGTTCGGATAGTTTTATCAGTAGCTCTAGTAAAATTGCTGAGATTAAAGAGCAT
TTTCCAGATGTTTTAGCCGTTGAAATGGAAGGGGCTTCAATTGCTCAAGCAGCAACAGCACTTGGAAAACCATTTGTAAT
TATTCGTGCGATGTCTGATACTGCTGACCATGATGCTAATGTTAATTTTGATAAATTTATTATTGAAGCAGGAAAAAAAT
CAGCTGAGGGCTTGATTCGTTTACTCAAAGAAATGGTATAA

Upstream 100 bases:

>100_bases
AATGGCTTTTCATCGTTACATTAATTGTTGTTCTATTGGCAGTAGCTTTCTTTTTGTACTACTTTTAGAGAGTAAAAAGA
TTAGAAAAAAGGTAAAAAAA

Downstream 100 bases:

>100_bases
AATTGGAATCTATCTTTTGATAGATTTTTCTTTTTATGAGAACTAAGAGGAAGTGAAATGGGAGAAATCAAGGATAAAAG
GTTAGAACTCTTAATTGAGG

Product: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase

Products: NA

Alternate protein names: MTA/SAH nucleosidase; MTAN; 5'-methylthioadenosine nucleosidase; MTA nucleosidase; S-adenosylhomocysteine nucleosidase; AdoHcy nucleosidase; SAH nucleosidase; SRH nucleosidase [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MKIGIICAMDEEIRILVENLENAEKHTRHGLVFHTGSIGRHEVVLVQSGIGKVMSALAVGFLVDIFDVDAIINTGSAGAV
AQGLAIGDVVVADKLAYHDVDVTAFGYAYGQMAQQPLYFESSKYFVSELKKVLENVHVGLITSSDSFISSSSKIAEIKEH
FPDVLAVEMEGASIAQAATALGKPFVIIRAMSDTADHDANVNFDKFIIEAGKKSAEGLIRLLKEMV

Sequences:

>Translated_226_residues
MKIGIICAMDEEIRILVENLENAEKHTRHGLVFHTGSIGRHEVVLVQSGIGKVMSALAVGFLVDIFDVDAIINTGSAGAV
AQGLAIGDVVVADKLAYHDVDVTAFGYAYGQMAQQPLYFESSKYFVSELKKVLENVHVGLITSSDSFISSSSKIAEIKEH
FPDVLAVEMEGASIAQAATALGKPFVIIRAMSDTADHDANVNFDKFIIEAGKKSAEGLIRLLKEMV
>Mature_226_residues
MKIGIICAMDEEIRILVENLENAEKHTRHGLVFHTGSIGRHEVVLVQSGIGKVMSALAVGFLVDIFDVDAIINTGSAGAV
AQGLAIGDVVVADKLAYHDVDVTAFGYAYGQMAQQPLYFESSKYFVSELKKVLENVHVGLITSSDSFISSSSKIAEIKEH
FPDVLAVEMEGASIAQAATALGKPFVIIRAMSDTADHDANVNFDKFIIEAGKKSAEGLIRLLKEMV

Specific function: Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S- adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively [H]

COG id: COG0775

COG function: function code F; Nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/UDP phosphorylase family. MtnN subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786354, Length=229, Percent_Identity=42.3580786026201, Blast_Score=167, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010049
- InterPro:   IPR018017
- InterPro:   IPR000845 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =3.2.2.9 [H]

Molecular weight: Translated: 24311; Mature: 24311

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGIICAMDEEIRILVENLENAEKHTRHGLVFHTGSIGRHEVVLVQSGIGKVMSALAVG
CEEEEEEECCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEEECCHHHHHHHHHHH
FLVDIFDVDAIINTGSAGAVAQGLAIGDVVVADKLAYHDVDVTAFGYAYGQMAQQPLYFE
HHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEC
SSKYFVSELKKVLENVHVGLITSSDSFISSSSKIAEIKEHFPDVLAVEMEGASIAQAATA
CCHHHHHHHHHHHHHCEEEEEECCCCHHCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHH
LGKPFVIIRAMSDTADHDANVNFDKFIIEAGKKSAEGLIRLLKEMV
CCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHC
>Mature Secondary Structure
MKIGIICAMDEEIRILVENLENAEKHTRHGLVFHTGSIGRHEVVLVQSGIGKVMSALAVG
CEEEEEEECCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEEECCHHHHHHHHHHH
FLVDIFDVDAIINTGSAGAVAQGLAIGDVVVADKLAYHDVDVTAFGYAYGQMAQQPLYFE
HHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEC
SSKYFVSELKKVLENVHVGLITSSDSFISSSSKIAEIKEHFPDVLAVEMEGASIAQAATA
CCHHHHHHHHHHHHHCEEEEEECCCCHHCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHH
LGKPFVIIRAMSDTADHDANVNFDKFIIEAGKKSAEGLIRLLKEMV
CCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA