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The map label for this gene is pnpA

Identifier: 15673837

GI number: 15673837

Start: 1921905

End: 1924226

Strand: Reverse

Name: pnpA

Synonym: L0325

Alternate gene names: 15673837

Gene position: 1924226-1921905 (Counterclockwise)

Preceding gene: 15673838

Following gene: 15673836

Centisome position: 81.34

GC content: 39.66

Gene sequence:

>2322_bases
TTGGCAAAAGAAACATTTTCAATCGAATTTGCAGGTCGGACATTAACTGTTGAAACAGGTCAAGTTGCAAAACAAGCAAA
TGGTGCTGTTGTTGTACGTTATGGAGATACAACTGTTTTAACAGCCGCTACAATGGGTAAAATGGCTACGGGTGATTTCT
TCCCACTTCAAGTGAACTATGAAGAAAAAATGTATGCTGCTGGTAAATTCCCAGGAGGTTTCAACAAACGTGAGGCGCGT
CCGTCGACTGATGCGACATTGACTGCTCGTTTAATTGACCGCCCAATTCGTCCTATGTTTGCTGAAGGTTTTCGTAATGA
AGTTCAAGTCATTAATACTGTTCTTTCTTATGATGAAAATGCTTCTGGTCGTGTGGCAGCAATGTTTGGCTCTTCTTTAG
CGCTTGCAATTTCTGATATTCCATTTGATAGACCAATTGCAGGTGTTGAAGTTGCCTATATTGATGGAGAATATATCATC
AATCCAACTGTTGCGGAAAAAGAGGCATCAAGTTTGGAGCTCTCTGTTGCGGGAAATATCAACGCAATCAATATGGTTGA
ATCTGGGGCCAAGGAACTTTCAGAAGAAGTGATGCTTGGTGCACTTTTGGCAGGACATAATGCTGTTAAAGAATTAATTG
AATTTCAAAATGAAATTGTTGCAAAAGTTGGAAAAGAAAAAGCTGAAGTTGAACTTTTGCATGTTGATGAAGATTTAAAA
GCCGAAGTGATTGCAGCTTACAATTCAGATTTGCAAAAAGCAGTTCAAATTGAAGAAAAATTGGCGCGTGAATCTGCGAC
AAAAGCAGTTAAAGAGGCGATAATTTCTGTTTACAGTGCAAAATATGAGAACGATGAAAATCTATCAATCATTTTGCGTG
ATCTTGCTGAAATTCTTGAAGGAATGGAACATGCGGAAGTTCGTCGCTTGATTACTGAAGATAAAATCCGTCCTGATGGA
CGTAAGATTGATGAAATTCGGCCGCTTGATGCTGAAATTGACTTTACCCCACGTAATATTACACATGGGACAGGTTTATT
TACTCGTGGTCAAACCCAAGCTTTATCAACATTGACTTTGGCACCAATGAATGAAGCACAAATCATTGATGGTTTGAATG
ATGAATATAAAAAACGTTTTATGCATCATTATAATTTCCCACAGTATTCTGTTGGGGAAACAGGACGTTACGGGGCACCT
GGACGTCGTGAAATTGGTCACGGAGCGCTCGGTGAACGAGCTTTAGAACAAGTATTGCCTTCTTTAGAGGAATTCCCTTA
TGCCATTCGTTTGGTCGCTGAAGTTTTAGAATCAAATGGTTCGTCATCACAAGCTTCAATCTGTGCAGGAACACTTGCAC
TGATGGCCGGTGGTGTACCAATCAAAGCACCAGTTGCTGGGATTGCGATGGGCTTGATTTCTGACGGAACAAATTATACT
GTTTTGACAGATATTCAAGGGCTTGAAGACCATTTTGGTGATATGGACTTCAAGGTTGCGGGAACTCGTGACGGGATTAC
TGCACTACAAATGGATATTAAAATTTCAGGAATTACACCAGAAATTCTTGCCGAAGCTCTTGCACAAGCAAAAACTGCAC
GTTTCCAAATTCTTGATGTAATTGAAGCAACAATTGCACAACCTCGTGAAGAGTTAGCACCTTCAGCACCTAAAATTGAT
ACAATTACAATTCCTGTCGATAAGATTAAAGTTGTCATCGGTAAAGGCGGCGAGCAAATCGATAAAATTATTGCTGAAAC
GGGCGTAAAAATTGATATTGATGACGAAGGACTTTGTTCAATTTTCTCTTCTGACCAAGCAGCGATTGATCGTGCTAAAG
AAATTATTGCTGAACTTGTTCGTGAAGCAAAAGTTGGTGAAATTTACGATGCAAAAGTGGTTCGTATCGAAAGTTTTGGT
GCTTTTGTAAATCTATTTGGTAAGCAAGATGCAATGGTGCATATTTCTGAAATGGCTTGGGCTCGGACAGAAAATGTTGA
AGATGTTGTGAAATTAGGTGACACTGTCAAAGTTAAAATCATGAAAATTGATGACAAGGGTCGCGTAGATGCATCAATGC
GTGCTTTAGTTGAGAAACCAGAAGGTTATGTTGAACCTGAGCGTAAACCAAGAGAACGCCGTGAAAATGGTGACCGTCGC
AAAGGTAACGGAAATAGTAACCGTGGTAATGGTTTTGACCGTCGTAATAATGACCGTAATAATCAGGGAAATAAAGTTGG
AAACCACAGTTTTGAGCTCCGTGAACGTAAATCACATATTGATGAAGAGTTTCCAGAACTCTCAACTAAAAAACCAGAAT
AA

Upstream 100 bases:

>100_bases
GATTTTTGTTTAGAAAAACATAAAGCTAAAATATATTACAAAAGCAGGAGACTGGACTGTTAGAGAAAGTTCATAGCATT
TCAAGTCAAGGAGAAAAACA

Downstream 100 bases:

>100_bases
GCTCAATCATAAAATGGAAAAGCTGTAAAAATAGACCTTGAAATAAAATAAAATTTACTGATTAAAATGCTAGCCTATCC
AGAAGTCATAGATGGCTAAT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 773; Mature: 772

Protein sequence:

>773_residues
MAKETFSIEFAGRTLTVETGQVAKQANGAVVVRYGDTTVLTAATMGKMATGDFFPLQVNYEEKMYAAGKFPGGFNKREAR
PSTDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDENASGRVAAMFGSSLALAISDIPFDRPIAGVEVAYIDGEYII
NPTVAEKEASSLELSVAGNINAINMVESGAKELSEEVMLGALLAGHNAVKELIEFQNEIVAKVGKEKAEVELLHVDEDLK
AEVIAAYNSDLQKAVQIEEKLARESATKAVKEAIISVYSAKYENDENLSIILRDLAEILEGMEHAEVRRLITEDKIRPDG
RKIDEIRPLDAEIDFTPRNITHGTGLFTRGQTQALSTLTLAPMNEAQIIDGLNDEYKKRFMHHYNFPQYSVGETGRYGAP
GRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGTLALMAGGVPIKAPVAGIAMGLISDGTNYT
VLTDIQGLEDHFGDMDFKVAGTRDGITALQMDIKISGITPEILAEALAQAKTARFQILDVIEATIAQPREELAPSAPKID
TITIPVDKIKVVIGKGGEQIDKIIAETGVKIDIDDEGLCSIFSSDQAAIDRAKEIIAELVREAKVGEIYDAKVVRIESFG
AFVNLFGKQDAMVHISEMAWARTENVEDVVKLGDTVKVKIMKIDDKGRVDASMRALVEKPEGYVEPERKPRERRENGDRR
KGNGNSNRGNGFDRRNNDRNNQGNKVGNHSFELRERKSHIDEEFPELSTKKPE

Sequences:

>Translated_773_residues
MAKETFSIEFAGRTLTVETGQVAKQANGAVVVRYGDTTVLTAATMGKMATGDFFPLQVNYEEKMYAAGKFPGGFNKREAR
PSTDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDENASGRVAAMFGSSLALAISDIPFDRPIAGVEVAYIDGEYII
NPTVAEKEASSLELSVAGNINAINMVESGAKELSEEVMLGALLAGHNAVKELIEFQNEIVAKVGKEKAEVELLHVDEDLK
AEVIAAYNSDLQKAVQIEEKLARESATKAVKEAIISVYSAKYENDENLSIILRDLAEILEGMEHAEVRRLITEDKIRPDG
RKIDEIRPLDAEIDFTPRNITHGTGLFTRGQTQALSTLTLAPMNEAQIIDGLNDEYKKRFMHHYNFPQYSVGETGRYGAP
GRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGTLALMAGGVPIKAPVAGIAMGLISDGTNYT
VLTDIQGLEDHFGDMDFKVAGTRDGITALQMDIKISGITPEILAEALAQAKTARFQILDVIEATIAQPREELAPSAPKID
TITIPVDKIKVVIGKGGEQIDKIIAETGVKIDIDDEGLCSIFSSDQAAIDRAKEIIAELVREAKVGEIYDAKVVRIESFG
AFVNLFGKQDAMVHISEMAWARTENVEDVVKLGDTVKVKIMKIDDKGRVDASMRALVEKPEGYVEPERKPRERRENGDRR
KGNGNSNRGNGFDRRNNDRNNQGNKVGNHSFELRERKSHIDEEFPELSTKKPE
>Mature_772_residues
AKETFSIEFAGRTLTVETGQVAKQANGAVVVRYGDTTVLTAATMGKMATGDFFPLQVNYEEKMYAAGKFPGGFNKREARP
STDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDENASGRVAAMFGSSLALAISDIPFDRPIAGVEVAYIDGEYIIN
PTVAEKEASSLELSVAGNINAINMVESGAKELSEEVMLGALLAGHNAVKELIEFQNEIVAKVGKEKAEVELLHVDEDLKA
EVIAAYNSDLQKAVQIEEKLARESATKAVKEAIISVYSAKYENDENLSIILRDLAEILEGMEHAEVRRLITEDKIRPDGR
KIDEIRPLDAEIDFTPRNITHGTGLFTRGQTQALSTLTLAPMNEAQIIDGLNDEYKKRFMHHYNFPQYSVGETGRYGAPG
RREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGTLALMAGGVPIKAPVAGIAMGLISDGTNYTV
LTDIQGLEDHFGDMDFKVAGTRDGITALQMDIKISGITPEILAEALAQAKTARFQILDVIEATIAQPREELAPSAPKIDT
ITIPVDKIKVVIGKGGEQIDKIIAETGVKIDIDDEGLCSIFSSDQAAIDRAKEIIAELVREAKVGEIYDAKVVRIESFGA
FVNLFGKQDAMVHISEMAWARTENVEDVVKLGDTVKVKIMKIDDKGRVDASMRALVEKPEGYVEPERKPRERRENGDRRK
GNGNSNRGNGFDRRNNDRNNQGNKVGNHSFELRERKSHIDEEFPELSTKKPE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=719, Percent_Identity=34.6314325452017, Blast_Score=404, Evalue=1e-112,
Organism=Escherichia coli, GI145693187, Length=696, Percent_Identity=48.4195402298851, Blast_Score=617, Evalue=1e-177,
Organism=Caenorhabditis elegans, GI115534063, Length=666, Percent_Identity=34.984984984985, Blast_Score=343, Evalue=3e-94,
Organism=Caenorhabditis elegans, GI17535281, Length=75, Percent_Identity=46.6666666666667, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281362905, Length=674, Percent_Identity=38.1305637982196, Blast_Score=422, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24651641, Length=674, Percent_Identity=38.1305637982196, Blast_Score=422, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24651643, Length=674, Percent_Identity=38.1305637982196, Blast_Score=422, Evalue=1e-118,
Organism=Drosophila melanogaster, GI161079377, Length=619, Percent_Identity=37.6413570274636, Blast_Score=389, Evalue=1e-108,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_LACLA (Q9CEI6)

Other databases:

- EMBL:   AE005176
- PIR:   G86856
- RefSeq:   NP_268012.1
- HSSP:   P05055
- ProteinModelPortal:   Q9CEI6
- SMR:   Q9CEI6
- GeneID:   1115520
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0325
- NMPDR:   fig|272623.1.peg.1901
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   LLAC272623:L0325-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 84671; Mature: 84540

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKETFSIEFAGRTLTVETGQVAKQANGAVVVRYGDTTVLTAATMGKMATGDFFPLQVNY
CCCCEEEEEECCCEEEEECCHHHHHCCCEEEEEECCCEEEEHHHHCCCCCCCEEEEEECC
EEKMYAAGKFPGGFNKREARPSTDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDEN
HHHEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
ASGRVAAMFGSSLALAISDIPFDRPIAGVEVAYIDGEYIINPTVAEKEASSLELSVAGNI
CCCEEEEEECCCEEEEEECCCCCCCCCCEEEEEECCCEEECCCCCCCCCCCEEEEECCCC
NAINMVESGAKELSEEVMLGALLAGHNAVKELIEFQNEIVAKVGKEKAEVELLHVDEDLK
CEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
AEVIAAYNSDLQKAVQIEEKLARESATKAVKEAIISVYSAKYENDENLSIILRDLAEILE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GMEHAEVRRLITEDKIRPDGRKIDEIRPLDAEIDFTPRNITHGTGLFTRGQTQALSTLTL
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCEECCCHHHHHEEEE
APMNEAQIIDGLNDEYKKRFMHHYNFPQYSVGETGRYGAPGRREIGHGALGERALEQVLP
CCCCHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
SLEEFPYAIRLVAEVLESNGSSSQASICAGTLALMAGGVPIKAPVAGIAMGLISDGTNYT
HHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEE
VLTDIQGLEDHFGDMDFKVAGTRDGITALQMDIKISGITPEILAEALAQAKTARFQILDV
EEEECCCHHHHCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
IEATIAQPREELAPSAPKIDTITIPVDKIKVVIGKGGEQIDKIIAETGVKIDIDDEGLCS
HHHHHCCCHHHHCCCCCCCEEEEECHHHEEEEECCCHHHHHHHHHHCCCEEEECCCCHHH
IFSSDQAAIDRAKEIIAELVREAKVGEIYDAKVVRIESFGAFVNLFGKQDAMVHISEMAW
HHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHCCCCCCEEEHHHHHH
ARTENVEDVVKLGDTVKVKIMKIDDKGRVDASMRALVEKPEGYVEPERKPRERRENGDRR
HHCCCHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCCCC
KGNGNSNRGNGFDRRNNDRNNQGNKVGNHSFELRERKSHIDEEFPELSTKKPE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AKETFSIEFAGRTLTVETGQVAKQANGAVVVRYGDTTVLTAATMGKMATGDFFPLQVNY
CCCEEEEEECCCEEEEECCHHHHHCCCEEEEEECCCEEEEHHHHCCCCCCCEEEEEECC
EEKMYAAGKFPGGFNKREARPSTDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDEN
HHHEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
ASGRVAAMFGSSLALAISDIPFDRPIAGVEVAYIDGEYIINPTVAEKEASSLELSVAGNI
CCCEEEEEECCCEEEEEECCCCCCCCCCEEEEEECCCEEECCCCCCCCCCCEEEEECCCC
NAINMVESGAKELSEEVMLGALLAGHNAVKELIEFQNEIVAKVGKEKAEVELLHVDEDLK
CEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
AEVIAAYNSDLQKAVQIEEKLARESATKAVKEAIISVYSAKYENDENLSIILRDLAEILE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GMEHAEVRRLITEDKIRPDGRKIDEIRPLDAEIDFTPRNITHGTGLFTRGQTQALSTLTL
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCEECCCHHHHHEEEE
APMNEAQIIDGLNDEYKKRFMHHYNFPQYSVGETGRYGAPGRREIGHGALGERALEQVLP
CCCCHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
SLEEFPYAIRLVAEVLESNGSSSQASICAGTLALMAGGVPIKAPVAGIAMGLISDGTNYT
HHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEE
VLTDIQGLEDHFGDMDFKVAGTRDGITALQMDIKISGITPEILAEALAQAKTARFQILDV
EEEECCCHHHHCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
IEATIAQPREELAPSAPKIDTITIPVDKIKVVIGKGGEQIDKIIAETGVKIDIDDEGLCS
HHHHHCCCHHHHCCCCCCCEEEEECHHHEEEEECCCHHHHHHHHHHCCCEEEECCCCHHH
IFSSDQAAIDRAKEIIAELVREAKVGEIYDAKVVRIESFGAFVNLFGKQDAMVHISEMAW
HHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHCCCCCCEEEHHHHHH
ARTENVEDVVKLGDTVKVKIMKIDDKGRVDASMRALVEKPEGYVEPERKPRERRENGDRR
HHCCCHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCCCC
KGNGNSNRGNGFDRRNNDRNNQGNKVGNHSFELRERKSHIDEEFPELSTKKPE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471