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The map label for this gene is thyA

Identifier: 15673523

GI number: 15673523

Start: 1582810

End: 1583649

Strand: Reverse

Name: thyA

Synonym: L182559

Alternate gene names: 15673523

Gene position: 1583649-1582810 (Counterclockwise)

Preceding gene: 15673524

Following gene: 15673522

Centisome position: 66.95

GC content: 35.24

Gene sequence:

>840_bases
ATGACTTACGCAGACAAGATTTTTAAACAAAATATCCAAAATATCCTTGATAACGGTGTTTTTTCAGAAAATGCACGTCC
CAAGTATAAGGATGGTCAAACCGCAAATAGTAAGTATGTAACAGGCTCTTTCGTTACTTATGATTTGCAAAAAGGTGAGT
TTCCAATTACCACTTTGCGTCCAATTCCAATTAAATCGGCAATTAAAGAATTGATGTGGATTTATCAAGATCAAACAAGT
GAACTTGCTATTCTTGAAGAAAAATATGGAGTCAAATACTGGGGCGAATGGGGAATTGGCGACGGTACGATTGGACAACG
CTATGGGGCAACAGTCAAAAAATATAATATTATTGGAAAATTATTGGATGGTTTAGCAAAAAATCCTTGGAATCGCCGTA
ATATCATTAATCTTTGGCAATATGAAGATTTTGAGGAAACTGAAGGACTTTTGCCTTGTGCTTTCCAAACAATGTTTGAT
GTTCGCCGTGAACAAGATGGTCAGATTTACTTGGATGCGACTCTTATTCAACGTTCAAATGATATGCTTGTTGCGCATCA
TATCAATGCCATGCAATATGTTGCTTTACAAATGATGATTGCAAAACATTTTTCTTGGAAAGTTGGAAAATTTTTCTATT
TCGTAAATAATTTGCATATTTATGATAATCAGTTTGAACAGGCAAATGAGTTAGTTAAGCGAACAGCTTCTGACAAGGAG
CCCCGTTTGGTGCTTAATGTTCCTGACGGAACAAACTTTTTCGATATTAAACCAGAAGATTTTGAGCTTGTGGACTATGA
ACCGGTAAAACCTCAATTAAAATTTGATTTGGCAATTTAG

Upstream 100 bases:

>100_bases
ATTAGTATCTTATTTTTTACAAGTAAATATTTGATAAATGCTATTGAATCAAAATAGCAGACTGTGTTATAATGAAAGGA
ATAAAAAGATAGGAAGATTC

Downstream 100 bases:

>100_bases
ATTAACCAAAAAGTTACTGACAGAACTCTGTCAGTAACTTTTTTATTGATAAAAATTTAAATATTGTTCGTTGACGAACA
AAAAAGATTGTGTTATAATA

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTYADKIFKQNIQNILDNGVFSENARPKYKDGQTANSKYVTGSFVTYDLQKGEFPITTLRPIPIKSAIKELMWIYQDQTS
ELAILEEKYGVKYWGEWGIGDGTIGQRYGATVKKYNIIGKLLDGLAKNPWNRRNIINLWQYEDFEETEGLLPCAFQTMFD
VRREQDGQIYLDATLIQRSNDMLVAHHINAMQYVALQMMIAKHFSWKVGKFFYFVNNLHIYDNQFEQANELVKRTASDKE
PRLVLNVPDGTNFFDIKPEDFELVDYEPVKPQLKFDLAI

Sequences:

>Translated_279_residues
MTYADKIFKQNIQNILDNGVFSENARPKYKDGQTANSKYVTGSFVTYDLQKGEFPITTLRPIPIKSAIKELMWIYQDQTS
ELAILEEKYGVKYWGEWGIGDGTIGQRYGATVKKYNIIGKLLDGLAKNPWNRRNIINLWQYEDFEETEGLLPCAFQTMFD
VRREQDGQIYLDATLIQRSNDMLVAHHINAMQYVALQMMIAKHFSWKVGKFFYFVNNLHIYDNQFEQANELVKRTASDKE
PRLVLNVPDGTNFFDIKPEDFELVDYEPVKPQLKFDLAI
>Mature_278_residues
TYADKIFKQNIQNILDNGVFSENARPKYKDGQTANSKYVTGSFVTYDLQKGEFPITTLRPIPIKSAIKELMWIYQDQTSE
LAILEEKYGVKYWGEWGIGDGTIGQRYGATVKKYNIIGKLLDGLAKNPWNRRNIINLWQYEDFEETEGLLPCAFQTMFDV
RREQDGQIYLDATLIQRSNDMLVAHHINAMQYVALQMMIAKHFSWKVGKFFYFVNNLHIYDNQFEQANELVKRTASDKEP
RLVLNVPDGTNFFDIKPEDFELVDYEPVKPQLKFDLAI

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=303, Percent_Identity=25.7425742574257, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789191, Length=282, Percent_Identity=27.6595744680851, Blast_Score=95, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6324648, Length=195, Percent_Identity=28.2051282051282, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17137556, Length=268, Percent_Identity=27.2388059701493, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_LACLA (P19368)

Other databases:

- EMBL:   M33770
- EMBL:   AE005176
- PIR:   A43797
- PIR:   E86817
- RefSeq:   NP_267697.1
- ProteinModelPortal:   P19368
- SMR:   P19368
- GeneID:   1115198
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L182559
- HOGENOM:   HBG588098
- OMA:   ENARPKY
- ProtClustDB:   PRK01827
- BioCyc:   LLAC272623:L182559-MONOMER
- BRENDA:   2.1.1.45
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 32499; Mature: 32368

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 153-153

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYADKIFKQNIQNILDNGVFSENARPKYKDGQTANSKYVTGSFVTYDLQKGEFPITTLR
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEEEC
PIPIKSAIKELMWIYQDQTSELAILEEKYGVKYWGEWGIGDGTIGQRYGATVKKYNIIGK
CCCHHHHHHHHHHHHCCCCCCEEEEHHHCCCEEEECCCCCCCCCCHHHCCHHHHHHHHHH
LLDGLAKNPWNRRNIINLWQYEDFEETEGLLPCAFQTMFDVRREQDGQIYLDATLIQRSN
HHHHHHCCCCCCCCCEEEECCCCHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCC
DMLVAHHINAMQYVALQMMIAKHFSWKVGKFFYFVNNLHIYDNQFEQANELVKRTASDKE
CEEEEEHHHHHHHHHHHHHHHHHCCCHHHEEEEEEEEEEEECCHHHHHHHHHHHHCCCCC
PRLVLNVPDGTNFFDIKPEDFELVDYEPVKPQLKFDLAI
CEEEEECCCCCCEEECCCCCCEEECCCCCCCCEEEEECC
>Mature Secondary Structure 
TYADKIFKQNIQNILDNGVFSENARPKYKDGQTANSKYVTGSFVTYDLQKGEFPITTLR
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEEEC
PIPIKSAIKELMWIYQDQTSELAILEEKYGVKYWGEWGIGDGTIGQRYGATVKKYNIIGK
CCCHHHHHHHHHHHHCCCCCCEEEEHHHCCCEEEECCCCCCCCCCHHHCCHHHHHHHHHH
LLDGLAKNPWNRRNIINLWQYEDFEETEGLLPCAFQTMFDVRREQDGQIYLDATLIQRSN
HHHHHHCCCCCCCCCEEEECCCCHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCC
DMLVAHHINAMQYVALQMMIAKHFSWKVGKFFYFVNNLHIYDNQFEQANELVKRTASDKE
CEEEEEHHHHHHHHHHHHHHHHHCCCHHHEEEEEEEEEEEECCHHHHHHHHHHHHCCCCC
PRLVLNVPDGTNFFDIKPEDFELVDYEPVKPQLKFDLAI
CEEEEECCCCCCEEECCCCCCEEECCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2117882; 11337471