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The map label for this gene is ypiB

Identifier: 15673521

GI number: 15673521

Start: 1580887

End: 1582260

Strand: Reverse

Name: ypiB

Synonym: L180636

Alternate gene names: 15673521

Gene position: 1582260-1580887 (Counterclockwise)

Preceding gene: 15673522

Following gene: 15673520

Centisome position: 66.89

GC content: 36.1

Gene sequence:

>1374_bases
ATGAACACAGAATTAGAAACAAATACAGCCAATCATAAAATTAGCGCGAAAACGCGCTTTTCAATTGTCGCCGTGGCCCT
TGTGGCTTTTATGGGGATATTAGCAGAAACAAGTTTGAATGTCACGTTTCCAACGCTTGAAAAGACCTTCGGACTTCCAC
TGAGTACGGTTCAGTGGGTAACGACCGCTTATTTATTAGTTGTTGCGATTGTTATGCCAACGAGTTCATATATTAGTCAA
CGCTTTCAAAATAGGACTTTATTTTTTTCAGGACTAATCTTTTTTACCCTTGGAGATTTACTGAGTTTAATTTCTCCGAA
TTTTACTATTTTAATGGCGGGACGCTTAATTCAAGGAATTGGGACTGGTATTGCCATTCCATTAATGTTTAATCTTGTCT
TAACTAAAATTCCGCGTGAGAGAGTAGGACTTTGGATGGGATTTGCTGGGATGATTTTGAGTATTGCCCCAGCACTTGGA
CCAACTTATGGTGGACTTTTAACAGGGACTTTAGGTTGGCGAATGATTTTTGCAATTATTTTGATTATCCCAATCTGTCT
CTTTCCTTTAGGAGCTTGGGCTATTGAGAAAAGTGAAAAAAAACGTCCAGGCAAATTTGATTTTATTGCTTTTATATTTT
TGTCATTAGCAATGACCAGCGCTCTTTTAGCTTTAGATTCACTTTCAAGTGCTCATTTTTCATGGATCTGGTTAGTGGTC
TTTATCTTGGGGTTGATTGCTTTTATTTGGCGTTCAAAAACAAGTACATTGAATTTTATTGATATCAATGTTTTTAAGAG
CCCAACTTTTACTTTGTCTGTCCTAGTTTATTGTATTTTACAATTTGTCAATTTGGGAATTAACTTTATCATTCCTAATT
TCTTACAAGTTTCGTTAGGAACTACAAGTACATTAGCAGGTTTTGCTTTATTGCCTGGTTCATTAATTGGTTCAGCAGCA
CAAGGTCCAATTGGTGCGCTCTATGACCGCAAAGGGGCCAAGGGGATTTTATATAGTGGAAATACTGTATTCTTTATCGC
TCTTGTTTTATTAACGATTTTTACTAAGAATCTGACTTTACTGGGTGTGATGGGACTTTATGTTGTATTCACAATCGGTC
GTTCTTCAAGTTTTGCAACAACAATGACCAATAGTTTAGCTCAACTTCCTCATGATAAAGCTGCCTCTGGTAATGCTTGG
TTTACTACAACTTCTCAATTTGCAGCGTCAGCAGGAACAGCTACAGCTAGTCTTATTGCAACAAAATCAAGTAATTTAAC
AACAGGAACTCAACATGTATTTATGTTTTTCCTTTGTCTTGCTTTGCTTAATTTTGTCTTCTATACTTTGATTTTGAACA
AGAAGATAAAATAA

Upstream 100 bases:

>100_bases
TCCAAGGTCATAATCAAAAAGCTTTGGCGGGTTTGTCAGCAGAGGAGATTGCTGCAGTTGAAAAATTTCTTAATAATTTT
AGCATGCAGAAAGGAAAAGA

Downstream 100 bases:

>100_bases
ATCTGATTAAAAAAGTTTCGTAATGATACGAAGCTTTTTTTATAATTTAATATAGTATGCTTAAAAAGCATAATTATTGA
TATAAAAAGCATTTCACGGC

Product: transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MNTELETNTANHKISAKTRFSIVAVALVAFMGILAETSLNVTFPTLEKTFGLPLSTVQWVTTAYLLVVAIVMPTSSYISQ
RFQNRTLFFSGLIFFTLGDLLSLISPNFTILMAGRLIQGIGTGIAIPLMFNLVLTKIPRERVGLWMGFAGMILSIAPALG
PTYGGLLTGTLGWRMIFAIILIIPICLFPLGAWAIEKSEKKRPGKFDFIAFIFLSLAMTSALLALDSLSSAHFSWIWLVV
FILGLIAFIWRSKTSTLNFIDINVFKSPTFTLSVLVYCILQFVNLGINFIIPNFLQVSLGTTSTLAGFALLPGSLIGSAA
QGPIGALYDRKGAKGILYSGNTVFFIALVLLTIFTKNLTLLGVMGLYVVFTIGRSSSFATTMTNSLAQLPHDKAASGNAW
FTTTSQFAASAGTATASLIATKSSNLTTGTQHVFMFFLCLALLNFVFYTLILNKKIK

Sequences:

>Translated_457_residues
MNTELETNTANHKISAKTRFSIVAVALVAFMGILAETSLNVTFPTLEKTFGLPLSTVQWVTTAYLLVVAIVMPTSSYISQ
RFQNRTLFFSGLIFFTLGDLLSLISPNFTILMAGRLIQGIGTGIAIPLMFNLVLTKIPRERVGLWMGFAGMILSIAPALG
PTYGGLLTGTLGWRMIFAIILIIPICLFPLGAWAIEKSEKKRPGKFDFIAFIFLSLAMTSALLALDSLSSAHFSWIWLVV
FILGLIAFIWRSKTSTLNFIDINVFKSPTFTLSVLVYCILQFVNLGINFIIPNFLQVSLGTTSTLAGFALLPGSLIGSAA
QGPIGALYDRKGAKGILYSGNTVFFIALVLLTIFTKNLTLLGVMGLYVVFTIGRSSSFATTMTNSLAQLPHDKAASGNAW
FTTTSQFAASAGTATASLIATKSSNLTTGTQHVFMFFLCLALLNFVFYTLILNKKIK
>Mature_457_residues
MNTELETNTANHKISAKTRFSIVAVALVAFMGILAETSLNVTFPTLEKTFGLPLSTVQWVTTAYLLVVAIVMPTSSYISQ
RFQNRTLFFSGLIFFTLGDLLSLISPNFTILMAGRLIQGIGTGIAIPLMFNLVLTKIPRERVGLWMGFAGMILSIAPALG
PTYGGLLTGTLGWRMIFAIILIIPICLFPLGAWAIEKSEKKRPGKFDFIAFIFLSLAMTSALLALDSLSSAHFSWIWLVV
FILGLIAFIWRSKTSTLNFIDINVFKSPTFTLSVLVYCILQFVNLGINFIIPNFLQVSLGTTSTLAGFALLPGSLIGSAA
QGPIGALYDRKGAKGILYSGNTVFFIALVLLTIFTKNLTLLGVMGLYVVFTIGRSSSFATTMTNSLAQLPHDKAASGNAW
FTTTSQFAASAGTATASLIATKSSNLTTGTQHVFMFFLCLALLNFVFYTLILNKKIK

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=421, Percent_Identity=25.1781472684086, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788392, Length=446, Percent_Identity=26.457399103139, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788710, Length=303, Percent_Identity=29.042904290429, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789042, Length=302, Percent_Identity=26.4900662251656, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87081983, Length=417, Percent_Identity=23.7410071942446, Blast_Score=72, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323735, Length=286, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49579; Mature: 49579

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTELETNTANHKISAKTRFSIVAVALVAFMGILAETSLNVTFPTLEKTFGLPLSTVQWV
CCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCHHHHHHH
TTAYLLVVAIVMPTSSYISQRFQNRTLFFSGLIFFTLGDLLSLISPNFTILMAGRLIQGI
HHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHH
GTGIAIPLMFNLVLTKIPRERVGLWMGFAGMILSIAPALGPTYGGLLTGTLGWRMIFAII
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LIIPICLFPLGAWAIEKSEKKRPGKFDFIAFIFLSLAMTSALLALDSLSSAHFSWIWLVV
HHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
FILGLIAFIWRSKTSTLNFIDINVFKSPTFTLSVLVYCILQFVNLGINFIIPNFLQVSLG
HHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCHHHCCCHHHEEECC
TTSTLAGFALLPGSLIGSAAQGPIGALYDRKGAKGILYSGNTVFFIALVLLTIFTKNLTL
CHHHHHHHHHCCHHHHCCCCCCCCHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCHHH
LGVMGLYVVFTIGRSSSFATTMTNSLAQLPHDKAASGNAWFTTTSQFAASAGTATASLIA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHCCCCHHHHHEE
TKSSNLTTGTQHVFMFFLCLALLNFVFYTLILNKKIK
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNTELETNTANHKISAKTRFSIVAVALVAFMGILAETSLNVTFPTLEKTFGLPLSTVQWV
CCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCHHHHHHH
TTAYLLVVAIVMPTSSYISQRFQNRTLFFSGLIFFTLGDLLSLISPNFTILMAGRLIQGI
HHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHH
GTGIAIPLMFNLVLTKIPRERVGLWMGFAGMILSIAPALGPTYGGLLTGTLGWRMIFAII
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LIIPICLFPLGAWAIEKSEKKRPGKFDFIAFIFLSLAMTSALLALDSLSSAHFSWIWLVV
HHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
FILGLIAFIWRSKTSTLNFIDINVFKSPTFTLSVLVYCILQFVNLGINFIIPNFLQVSLG
HHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCHHHCCCHHHEEECC
TTSTLAGFALLPGSLIGSAAQGPIGALYDRKGAKGILYSGNTVFFIALVLLTIFTKNLTL
CHHHHHHHHHCCHHHHCCCCCCCCHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCHHH
LGVMGLYVVFTIGRSSSFATTMTNSLAQLPHDKAASGNAWFTTTSQFAASAGTATASLIA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHCCCCHHHHHEE
TKSSNLTTGTQHVFMFFLCLALLNFVFYTLILNKKIK
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]