Click here to switch to the map view.

The map label for this gene is purC

Identifier: 15673515

GI number: 15673515

Start: 1577601

End: 1578311

Strand: Reverse

Name: purC

Synonym: L177350

Alternate gene names: 15673515

Gene position: 1578311-1577601 (Counterclockwise)

Preceding gene: 15673516

Following gene: 15673514

Centisome position: 66.72

GC content: 33.47

Gene sequence:

>711_bases
GTGGAAAAAGAAAAGTTGCTCTATGAAGGAAAAGCTAAGAAACTGTATTTTACTGATGATTCAGAGGTGCTCTGGGTGGA
ATATTGTGACCAAGCGACTGCATTAAATGGTGCTCGTAAAGAACAGATTACGGGAAAAGGTGCTCTAAATAATCAAATTA
CGTCATTAATTTTTGAAAAATTAAATGCTGAAGGTTTAGAAACGCATTTCATAGAAAAGTTATCAAAAACCGAACAATTG
AATAGAAAAGTTTCAATAATTCCTTTAGAAGTTGTTTTAAGAAATGTTGTTGCTGGTTCTTTTGCCAAACGATTTGGTCT
TGAAGAAGGAATTGTTTTACAAGAACCAATTGTTGAGTTTTACTATAAGGATGATGCTTTAGATGACCCATTTATTAATG
ATGAACATGTTAGATTCCTGAACATTGCAACTTATTCAGAGATTGAATTTTTAAAGAGTGAAACGCGTAAAATCAACGAG
ATTCTTAAAAAAATATGGGCTGAGATCGGCTTAACTTTGGTTGATTTCAAACTTGAATTTGGCCGCCTTGCTGACGGAAG
AATTATTCTTGCTGATGAAATTTCACCTGATACTTCACGTCTCTGGGATGCGAATGGTCAGCATATGGATAAAGATGTTT
TCAGGCGTAATATTGGTGATTTAGTTGAAACTTATACTGAAGTTTTGAATTTGTTAGAAAATGCGAAATAA

Upstream 100 bases:

>100_bases
ATTGAAAAAGTTCTAGTTTTATCCGCTAGAGCTTTTTTTATTTGGCTCATTAGAATAGAATAAGAAGCATAAAGAATCCC
TTAAATGGATGGAGGATATT

Downstream 100 bases:

>100_bases
ATGAGTTCTAGTTTTTAGGACAGTTCCTCACATCTTATATAGAAAGAATTTTAAAATGACTAAAGTACGTGTTTACGTGG
CTTACAAAGCCTCAATTCTT

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MEKEKLLYEGKAKKLYFTDDSEVLWVEYCDQATALNGARKEQITGKGALNNQITSLIFEKLNAEGLETHFIEKLSKTEQL
NRKVSIIPLEVVLRNVVAGSFAKRFGLEEGIVLQEPIVEFYYKDDALDDPFINDEHVRFLNIATYSEIEFLKSETRKINE
ILKKIWAEIGLTLVDFKLEFGRLADGRIILADEISPDTSRLWDANGQHMDKDVFRRNIGDLVETYTEVLNLLENAK

Sequences:

>Translated_236_residues
MEKEKLLYEGKAKKLYFTDDSEVLWVEYCDQATALNGARKEQITGKGALNNQITSLIFEKLNAEGLETHFIEKLSKTEQL
NRKVSIIPLEVVLRNVVAGSFAKRFGLEEGIVLQEPIVEFYYKDDALDDPFINDEHVRFLNIATYSEIEFLKSETRKINE
ILKKIWAEIGLTLVDFKLEFGRLADGRIILADEISPDTSRLWDANGQHMDKDVFRRNIGDLVETYTEVLNLLENAK
>Mature_236_residues
MEKEKLLYEGKAKKLYFTDDSEVLWVEYCDQATALNGARKEQITGKGALNNQITSLIFEKLNAEGLETHFIEKLSKTEQL
NRKVSIIPLEVVLRNVVAGSFAKRFGLEEGIVLQEPIVEFYYKDDALDDPFINDEHVRFLNIATYSEIEFLKSETRKINE
ILKKIWAEIGLTLVDFKLEFGRLADGRIILADEISPDTSRLWDANGQHMDKDVFRRNIGDLVETYTEVLNLLENAK

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family

Homologues:

Organism=Homo sapiens, GI119220557, Length=238, Percent_Identity=36.9747899159664, Blast_Score=138, Evalue=4e-33,
Organism=Homo sapiens, GI5453539, Length=238, Percent_Identity=36.9747899159664, Blast_Score=138, Evalue=4e-33,
Organism=Homo sapiens, GI119220559, Length=245, Percent_Identity=35.5102040816327, Blast_Score=129, Evalue=3e-30,
Organism=Escherichia coli, GI1788820, Length=229, Percent_Identity=42.7947598253275, Blast_Score=189, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17531275, Length=248, Percent_Identity=35.0806451612903, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI18860083, Length=221, Percent_Identity=33.0316742081448, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24583917, Length=220, Percent_Identity=32.2727272727273, Blast_Score=113, Evalue=1e-25,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PUR7_LACLA (O68830)

Other databases:

- EMBL:   AE005176
- EMBL:   AF054888
- PIR:   E86816
- RefSeq:   NP_267689.1
- ProteinModelPortal:   O68830
- SMR:   O68830
- GeneID:   1115190
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L177350
- NMPDR:   fig|272623.1.peg.1574
- HOGENOM:   HBG306070
- OMA:   YKDDALG
- ProtClustDB:   PRK09362
- BioCyc:   LLAC272623:L177350-MONOMER
- BRENDA:   6.3.2.6
- HAMAP:   MF_00137
- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236
- Gene3D:   G3DSA:3.30.470.20
- PANTHER:   PTHR11609
- TIGRFAMs:   TIGR00081

Pfam domain/function: PF01259 SAICAR_synt

EC number: =6.3.2.6

Molecular weight: Translated: 27136; Mature: 27136

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS01057 SAICAR_SYNTHETASE_1; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKEKLLYEGKAKKLYFTDDSEVLWVEYCDQATALNGARKEQITGKGALNNQITSLIFEK
CCCCHHEECCCCCEEEEECCCCEEEEECCCHHHHCCCCHHHHCCCCCCHHHHHHHHHHHH
LNAEGLETHFIEKLSKTEQLNRKVSIIPLEVVLRNVVAGSFAKRFGLEEGIVLQEPIVEF
HCCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
YYKDDALDDPFINDEHVRFLNIATYSEIEFLKSETRKINEILKKIWAEIGLTLVDFKLEF
HCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECC
GRLADGRIILADEISPDTSRLWDANGQHMDKDVFRRNIGDLVETYTEVLNLLENAK
CCCCCCEEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKEKLLYEGKAKKLYFTDDSEVLWVEYCDQATALNGARKEQITGKGALNNQITSLIFEK
CCCCHHEECCCCCEEEEECCCCEEEEECCCHHHHCCCCHHHHCCCCCCHHHHHHHHHHHH
LNAEGLETHFIEKLSKTEQLNRKVSIIPLEVVLRNVVAGSFAKRFGLEEGIVLQEPIVEF
HCCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
YYKDDALDDPFINDEHVRFLNIATYSEIEFLKSETRKINEILKKIWAEIGLTLVDFKLEF
HCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECC
GRLADGRIILADEISPDTSRLWDANGQHMDKDVFRRNIGDLVETYTEVLNLLENAK
CCCCCCEEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471; 9683487