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The map label for this gene is purH

Identifier: 15673500

GI number: 15673500

Start: 1558961

End: 1560517

Strand: Reverse

Name: purH

Synonym: L158710

Alternate gene names: 15673500

Gene position: 1560517-1558961 (Counterclockwise)

Preceding gene: 15673501

Following gene: 15673499

Centisome position: 65.97

GC content: 38.73

Gene sequence:

>1557_bases
ATGTCAAAACGTGCACTTATCAGTGTTTCAGACAAAGAAGGTATTGTAGAATTTGCTAAGGAAATTCGAGAATTAGGTTG
GGAAATTATCTCAACGGGTGGAACGAAAGCCGTTCTGGACCAAGAAGAAATTCCGAATATTGCAATTGATGAAGTAACAG
GATTTCCAGAAATGATGGATGGGCGCCTTAAAACATTGCATCCACTAATTCATGGAGCCCTTCTTGGACGTAGAGACTTG
GAAAGTCACATGAAATCAATGACAGAACATCATATTTCTCCAATTGATTTGGTTGTTGTAAACTTATATCCTTTCAAAGA
AACTTTGCTTGCTGGGGGCAGTCAAGCAGAAATGATTGAGAAGATTGATATTGGTGGTCCTTCAATGTTGCGTTCAGCAG
CTAAAAACCATGCGTCAGTCACAGTCGTTTGTGACCCAAATGATTATGAAAAAGTACTGACAGAGCTGTCAGTAAAAGGC
GAAACAAGTTTAAGTTTTCGTCAGCAACTTGCAGCAAAAGTATTTCGCCACACTGCAAGTTATGATGCTTTAATTGCACA
ATATTTGACAGAAGAATTTCCGGTAGAAAATGTAAAACCAGAAAAATTAACCTTGACTTACGACCTTAAACAAGGAATGC
GTTATGGTGAAAATCCGCAACAAAATGCAGATTTTTATGAGTCAGGAATACCTACGACTTATTCAATTGCCCAAAGTAAG
CAAATTCATGGTAAAGAACTAAGCTATAACAACGTTCGTGATGCAGATGCTGCTTTACAGATTGCAAGAGATTTTGAAGA
ACCAACAGTTGTAGCCCTCAAACATATGAATCCATGTGGGATTGGAACAGCAACGGATATCGAAAAAGCTTGGGATTATG
CCTATGAAGCAGACCCAGTTTCAATTTTCGGTGGAATTATCGTTTTAAATCGAGAAGTAACTTTGGCAACTGCGCAAAAA
ATGAGTAAGATTTTCCTTGAAATTATTATCGCACCCTCTTATAGCAAAGAAGCCTTGGAAGTTTTAAGTAAAAAGAAAAA
TATTCGACTTTTAACCGTTGATTTTTCTAAAAAAGAAGCCTCAGAAAAAGAAGCGCTTGTAACAGGGGTTCTTGGAGGAC
TACTTGTTCAAAATCAAGATGTCATTGTTGAAAATCCAAAAGAATGGACAGTGGCGACTAAAGTTCAACCAACTGATAGA
CAAATGGAGGCCATGAAGTTTGCTTGGAAAGCAGTTAAGTTTGTCAAATCAAATGGGATTATTGTGACCAATGACCACCA
GACGCTTGGTGTTGGTCCTGGCCAAACTAACCGTGTGGGCTCAGTAAAAATTGCCTTAGAGGCAACCGCAGATAAATCAG
TAGAACTTCAAGAAAATGCGGTTCTTGGTTCGGATGCCTTTTTCCCATTTGCTGACAATATCGATGAAATTGCTAAGGCA
GGAATTAAAGCAATTGTTCAACCAGGTGGCTCTGTACGAGACCAAGAAGTGATTGATGCCTGTGATAAATATGGAATTGC
GATGGTCTTCACAGGACTTCGTCATTTTAGACATTAA

Upstream 100 bases:

>100_bases
TAAAATAAAGACATGATAAATATTTACTGACAGTTCTGTCAGTTCTTTTATAAGAAAAATATTACTGACAGAATTGTCAG
CAGAAAACTTGGAGGGAATC

Downstream 100 bases:

>100_bases
TAAAAAGTCTACCTTTAGGGTAGATTTTTTTCTCTAATTTAATATTAAAATTTCTATTGTAAAATCATCTGTCTATTGTC
ATTGAAAAAATTTTCAAGTT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MSKRALISVSDKEGIVEFAKEIRELGWEIISTGGTKAVLDQEEIPNIAIDEVTGFPEMMDGRLKTLHPLIHGALLGRRDL
ESHMKSMTEHHISPIDLVVVNLYPFKETLLAGGSQAEMIEKIDIGGPSMLRSAAKNHASVTVVCDPNDYEKVLTELSVKG
ETSLSFRQQLAAKVFRHTASYDALIAQYLTEEFPVENVKPEKLTLTYDLKQGMRYGENPQQNADFYESGIPTTYSIAQSK
QIHGKELSYNNVRDADAALQIARDFEEPTVVALKHMNPCGIGTATDIEKAWDYAYEADPVSIFGGIIVLNREVTLATAQK
MSKIFLEIIIAPSYSKEALEVLSKKKNIRLLTVDFSKKEASEKEALVTGVLGGLLVQNQDVIVENPKEWTVATKVQPTDR
QMEAMKFAWKAVKFVKSNGIIVTNDHQTLGVGPGQTNRVGSVKIALEATADKSVELQENAVLGSDAFFPFADNIDEIAKA
GIKAIVQPGGSVRDQEVIDACDKYGIAMVFTGLRHFRH

Sequences:

>Translated_518_residues
MSKRALISVSDKEGIVEFAKEIRELGWEIISTGGTKAVLDQEEIPNIAIDEVTGFPEMMDGRLKTLHPLIHGALLGRRDL
ESHMKSMTEHHISPIDLVVVNLYPFKETLLAGGSQAEMIEKIDIGGPSMLRSAAKNHASVTVVCDPNDYEKVLTELSVKG
ETSLSFRQQLAAKVFRHTASYDALIAQYLTEEFPVENVKPEKLTLTYDLKQGMRYGENPQQNADFYESGIPTTYSIAQSK
QIHGKELSYNNVRDADAALQIARDFEEPTVVALKHMNPCGIGTATDIEKAWDYAYEADPVSIFGGIIVLNREVTLATAQK
MSKIFLEIIIAPSYSKEALEVLSKKKNIRLLTVDFSKKEASEKEALVTGVLGGLLVQNQDVIVENPKEWTVATKVQPTDR
QMEAMKFAWKAVKFVKSNGIIVTNDHQTLGVGPGQTNRVGSVKIALEATADKSVELQENAVLGSDAFFPFADNIDEIAKA
GIKAIVQPGGSVRDQEVIDACDKYGIAMVFTGLRHFRH
>Mature_517_residues
SKRALISVSDKEGIVEFAKEIRELGWEIISTGGTKAVLDQEEIPNIAIDEVTGFPEMMDGRLKTLHPLIHGALLGRRDLE
SHMKSMTEHHISPIDLVVVNLYPFKETLLAGGSQAEMIEKIDIGGPSMLRSAAKNHASVTVVCDPNDYEKVLTELSVKGE
TSLSFRQQLAAKVFRHTASYDALIAQYLTEEFPVENVKPEKLTLTYDLKQGMRYGENPQQNADFYESGIPTTYSIAQSKQ
IHGKELSYNNVRDADAALQIARDFEEPTVVALKHMNPCGIGTATDIEKAWDYAYEADPVSIFGGIIVLNREVTLATAQKM
SKIFLEIIIAPSYSKEALEVLSKKKNIRLLTVDFSKKEASEKEALVTGVLGGLLVQNQDVIVENPKEWTVATKVQPTDRQ
MEAMKFAWKAVKFVKSNGIIVTNDHQTLGVGPGQTNRVGSVKIALEATADKSVELQENAVLGSDAFFPFADNIDEIAKAG
IKAIVQPGGSVRDQEVIDACDKYGIAMVFTGLRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=475, Percent_Identity=34.9473684210526, Blast_Score=229, Evalue=4e-60,
Organism=Escherichia coli, GI1790439, Length=537, Percent_Identity=46.3687150837989, Blast_Score=479, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI71985564, Length=614, Percent_Identity=29.6416938110749, Blast_Score=239, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI71985556, Length=62, Percent_Identity=48.3870967741936, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71985574, Length=309, Percent_Identity=22.9773462783171, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6323768, Length=477, Percent_Identity=33.5429769392034, Blast_Score=232, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6323056, Length=479, Percent_Identity=32.9853862212944, Blast_Score=231, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24649832, Length=477, Percent_Identity=32.2851153039832, Blast_Score=226, Evalue=4e-59,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_LACLA (Q9CFG0)

Other databases:

- EMBL:   AE005176
- PIR:   F86814
- RefSeq:   NP_267674.1
- ProteinModelPortal:   Q9CFG0
- SMR:   Q9CFG0
- GeneID:   1115175
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L158710
- NMPDR:   fig|272623.1.peg.1559
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- ProtClustDB:   PRK00881
- BioCyc:   LLAC272623:L158710-MONOMER
- BRENDA:   2.1.2.3
- BRENDA:   3.5.4.10
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 57095; Mature: 56964

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRALISVSDKEGIVEFAKEIRELGWEIISTGGTKAVLDQEEIPNIAIDEVTGFPEMMD
CCCCEEEEECCCCHHHHHHHHHHHHCHHEECCCCCEEEECHHHCCCEEHHHHCCCHHHHC
GRLKTLHPLIHGALLGRRDLESHMKSMTEHHISPIDLVVVNLYPFKETLLAGGSQAEMIE
CHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHCCCCHHHHHH
KIDIGGPSMLRSAAKNHASVTVVCDPNDYEKVLTELSVKGETSLSFRQQLAAKVFRHTAS
HHCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCH
YDALIAQYLTEEFPVENVKPEKLTLTYDLKQGMRYGENPQQNADFYESGIPTTYSIAQSK
HHHHHHHHHHHHCCCCCCCCCEEEEEEEHHHCCCCCCCCCCCCCHHHCCCCCEEEHHHCC
QIHGKELSYNNVRDADAALQIARDFEEPTVVALKHMNPCGIGTATDIEKAWDYAYEADPV
CCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCCH
SIFGGIIVLNREVTLATAQKMSKIFLEIIIAPSYSKEALEVLSKKKNIRLLTVDFSKKEA
HEECCEEEEECCCHHHHHHHHHHHHHHHEECCCCCHHHHHHHHCCCCEEEEEEECCHHHC
SEKEALVTGVLGGLLVQNQDVIVENPKEWTVATKVQPTDRQMEAMKFAWKAVKFVKSNGI
HHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCE
IVTNDHQTLGVGPGQTNRVGSVKIALEATADKSVELQENAVLGSDAFFPFADNIDEIAKA
EEECCCCEEECCCCCCCCCCEEEEEEEECCCCCEEECCCEEECCCCCCCCCCCHHHHHHH
GIKAIVQPGGSVRDQEVIDACDKYGIAMVFTGLRHFRH
HHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SKRALISVSDKEGIVEFAKEIRELGWEIISTGGTKAVLDQEEIPNIAIDEVTGFPEMMD
CCCEEEEECCCCHHHHHHHHHHHHCHHEECCCCCEEEECHHHCCCEEHHHHCCCHHHHC
GRLKTLHPLIHGALLGRRDLESHMKSMTEHHISPIDLVVVNLYPFKETLLAGGSQAEMIE
CHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHCCCCHHHHHH
KIDIGGPSMLRSAAKNHASVTVVCDPNDYEKVLTELSVKGETSLSFRQQLAAKVFRHTAS
HHCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCH
YDALIAQYLTEEFPVENVKPEKLTLTYDLKQGMRYGENPQQNADFYESGIPTTYSIAQSK
HHHHHHHHHHHHCCCCCCCCCEEEEEEEHHHCCCCCCCCCCCCCHHHCCCCCEEEHHHCC
QIHGKELSYNNVRDADAALQIARDFEEPTVVALKHMNPCGIGTATDIEKAWDYAYEADPV
CCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCCH
SIFGGIIVLNREVTLATAQKMSKIFLEIIIAPSYSKEALEVLSKKKNIRLLTVDFSKKEA
HEECCEEEEECCCHHHHHHHHHHHHHHHEECCCCCHHHHHHHHCCCCEEEEEEECCHHHC
SEKEALVTGVLGGLLVQNQDVIVENPKEWTVATKVQPTDRQMEAMKFAWKAVKFVKSNGI
HHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCE
IVTNDHQTLGVGPGQTNRVGSVKIALEATADKSVELQENAVLGSDAFFPFADNIDEIAKA
EEECCCCEEECCCCCCCCCCEEEEEEEECCCCCEEECCCEEECCCCCCCCCCCHHHHHHH
GIKAIVQPGGSVRDQEVIDACDKYGIAMVFTGLRHFRH
HHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471