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The map label for this gene is hsdM

Identifier: 15672633

GI number: 15672633

Start: 643807

End: 645354

Strand: Direct

Name: hsdM

Synonym: L0309

Alternate gene names: 15672633

Gene position: 643807-645354 (Clockwise)

Preceding gene: 15672632

Following gene: 15672634

Centisome position: 27.22

GC content: 39.79

Gene sequence:

>1548_bases
ATGGCATTATCAAATGAACAAAAAAATAAAATGTGGGCCCTCTTAAATCAAACCCGAGGACAGATTGGTTTAACCGCCTA
TAAAGACTACATTTTTGGCTTACTATTTTATAAATATTTATCAGAAAAGGCCACACAATGGCTGGGAGAAGTTTTACGAG
GAGACACTTGGGAAAATGTTTATGATCAAGATCCTGTAAGAGCCTTAGACTATATGAAACAAAAGCTCGGTTATGCCATC
CAACCGAAGGAATTTTTTAAAGATTGGGAAGCTGCCATTCATGAAGAACGATTTAACATTCCAATGATTTCAGATACATT
TGGCCATTTTAATCAGCAAATTGCTTTTGAAGCTAAAGATGATTTTGAAGGCATCTTTGATGGGATGCGTTTTGATAGTT
CAGATTTAGGAAGTAATGCGCAAGCTCGAGCAAGTGTGATGATCTCGATGATTGAGTTACTCTCTGCCCCTGAGTTTGAT
CTCTCTACAGGAGGGGACACCGTTTCAGATATCTACGAATACTTGTTAGAAAAGTTTGCGACCGTATTGGCCTCAGACAT
GGGACAATACTATACTCCCAAAGAAATTTCAGAGGTCATGGCTCGTATTTTGACTTTTGGTAAAGCCGACGAAGACAATT
TTTCTATTTATGATCCCGCGGTCGGTTCAGCTTCTCTCTTAATTACTACCGCCAGTCATATGAAACATTCCAATCAAAGG
GGAGCGATTAAATACTTTGGTCAAGAAAAAGATGCCACCCCTTACCGATTGGCTCGAATGAATTTAATGATGCATAACAT
TGAATACAATGATATTCAGATCCATCATGCGGATACTTTGGAAAGCGATTGGCCTGACGGAGTCATTGAAGGGAAAGATA
CGCCTCGAATGTTTGACGCAGTGATGGCTAATCCACCTTATTCTGCCCATTGGAATAATAAGGATCGGGAAGATGACCCT
CGATTTCGTGAATATGGCATTGCGCCAAAAACTAAAGCGGACTATTCTTTCTTGTTGCATTGTTTGTACCATACCAAAGA
GAGTGGCCGTGTGGCCATTATTCTCCCACACGGGGTCTTGTTTAGAGGCGCTGCGGAAGGACGGATTCGAAAAGCTTTGA
TTGATAAACATCAAATTGAAGCGGTAATTGGTTTTCCAGATAAACTCTTTTTGAACACAGGCATTCCCGTTTGTGTCTTA
ATTCTTAAAAAGAATCGAGCAAACTCTGATATTCTTTTTGTGGACGCCAGTCAAGGCTTTGAAAAGATGAAGAATCAAAA
ACAATTGCGCCCAGAAGATATTGACAAGATCACTGAAACAGTCATTCATCGCAAGGCGGTTGATAAATATAGCCACTTAG
CAACGCTTGAGGAAGTCATTGAGAATGATTACAACTTAAATATTCCGCGTTATGTGGATACCTTTGAAGAGGAAGAATCG
ATTGACTTAGCGGATATTCAAGGTCAAATAGATGAAGTAGACGCAGAGATTGCGAAAGCTAATCAAACCTTAGCAAACTA
CTTTAAGGAACTGGGAGTGCTAAAATGA

Upstream 100 bases:

>100_bases
AAGCTTTTTTGGAGCCTCAGAATTTGAGACTGCATTAATGAGCTTCTTCAATTCACTATAAAAAAATAAAAATACTCGCA
AGAAATAAGGAGCGAATAAA

Downstream 100 bases:

>100_bases
AGGAAAGATTAAAGGCTCCAGAATTAAGGTTTGATGGTTTTACGGATGATTGGGAAGAGCGTAAGTTATTAGACAATGTA
GAAAAAGTATTAGATTATCG

Product: type I restriction enzyme M protein

Products: NA

Alternate protein names: M.MpnORFDP [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MALSNEQKNKMWALLNQTRGQIGLTAYKDYIFGLLFYKYLSEKATQWLGEVLRGDTWENVYDQDPVRALDYMKQKLGYAI
QPKEFFKDWEAAIHEERFNIPMISDTFGHFNQQIAFEAKDDFEGIFDGMRFDSSDLGSNAQARASVMISMIELLSAPEFD
LSTGGDTVSDIYEYLLEKFATVLASDMGQYYTPKEISEVMARILTFGKADEDNFSIYDPAVGSASLLITTASHMKHSNQR
GAIKYFGQEKDATPYRLARMNLMMHNIEYNDIQIHHADTLESDWPDGVIEGKDTPRMFDAVMANPPYSAHWNNKDREDDP
RFREYGIAPKTKADYSFLLHCLYHTKESGRVAIILPHGVLFRGAAEGRIRKALIDKHQIEAVIGFPDKLFLNTGIPVCVL
ILKKNRANSDILFVDASQGFEKMKNQKQLRPEDIDKITETVIHRKAVDKYSHLATLEEVIENDYNLNIPRYVDTFEEEES
IDLADIQGQIDEVDAEIAKANQTLANYFKELGVLK

Sequences:

>Translated_515_residues
MALSNEQKNKMWALLNQTRGQIGLTAYKDYIFGLLFYKYLSEKATQWLGEVLRGDTWENVYDQDPVRALDYMKQKLGYAI
QPKEFFKDWEAAIHEERFNIPMISDTFGHFNQQIAFEAKDDFEGIFDGMRFDSSDLGSNAQARASVMISMIELLSAPEFD
LSTGGDTVSDIYEYLLEKFATVLASDMGQYYTPKEISEVMARILTFGKADEDNFSIYDPAVGSASLLITTASHMKHSNQR
GAIKYFGQEKDATPYRLARMNLMMHNIEYNDIQIHHADTLESDWPDGVIEGKDTPRMFDAVMANPPYSAHWNNKDREDDP
RFREYGIAPKTKADYSFLLHCLYHTKESGRVAIILPHGVLFRGAAEGRIRKALIDKHQIEAVIGFPDKLFLNTGIPVCVL
ILKKNRANSDILFVDASQGFEKMKNQKQLRPEDIDKITETVIHRKAVDKYSHLATLEEVIENDYNLNIPRYVDTFEEEES
IDLADIQGQIDEVDAEIAKANQTLANYFKELGVLK
>Mature_514_residues
ALSNEQKNKMWALLNQTRGQIGLTAYKDYIFGLLFYKYLSEKATQWLGEVLRGDTWENVYDQDPVRALDYMKQKLGYAIQ
PKEFFKDWEAAIHEERFNIPMISDTFGHFNQQIAFEAKDDFEGIFDGMRFDSSDLGSNAQARASVMISMIELLSAPEFDL
STGGDTVSDIYEYLLEKFATVLASDMGQYYTPKEISEVMARILTFGKADEDNFSIYDPAVGSASLLITTASHMKHSNQRG
AIKYFGQEKDATPYRLARMNLMMHNIEYNDIQIHHADTLESDWPDGVIEGKDTPRMFDAVMANPPYSAHWNNKDREDDPR
FREYGIAPKTKADYSFLLHCLYHTKESGRVAIILPHGVLFRGAAEGRIRKALIDKHQIEAVIGFPDKLFLNTGIPVCVLI
LKKNRANSDILFVDASQGFEKMKNQKQLRPEDIDKITETVIHRKAVDKYSHLATLEEVIENDYNLNIPRYVDTFEEEESI
DLADIQGQIDEVDAEIAKANQTLANYFKELGVLK

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=255, Percent_Identity=28.2352941176471, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 58856; Mature: 58725

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSNEQKNKMWALLNQTRGQIGLTAYKDYIFGLLFYKYLSEKATQWLGEVLRGDTWENV
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
YDQDPVRALDYMKQKLGYAIQPKEFFKDWEAAIHEERFNIPMISDTFGHFNQQIAFEAKD
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEECCCH
DFEGIFDGMRFDSSDLGSNAQARASVMISMIELLSAPEFDLSTGGDTVSDIYEYLLEKFA
HHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
TVLASDMGQYYTPKEISEVMARILTFGKADEDNFSIYDPAVGSASLLITTASHMKHSNQR
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEEEEEEHHHHHCCCCC
GAIKYFGQEKDATPYRLARMNLMMHNIEYNDIQIHHADTLESDWPDGVIEGKDTPRMFDA
CCEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHH
VMANPPYSAHWNNKDREDDPRFREYGIAPKTKADYSFLLHCLYHTKESGRVAIILPHGVL
HHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCEE
FRGAAEGRIRKALIDKHQIEAVIGFPDKLFLNTGIPVCVLILKKNRANSDILFVDASQGF
ECCCCHHHHHHHHHHHHHHHEEECCCHHHEECCCCCEEEEEEECCCCCCCEEEEECHHHH
EKMKNQKQLRPEDIDKITETVIHRKAVDKYSHLATLEEVIENDYNLNIPRYVDTFEEEES
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCC
IDLADIQGQIDEVDAEIAKANQTLANYFKELGVLK
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALSNEQKNKMWALLNQTRGQIGLTAYKDYIFGLLFYKYLSEKATQWLGEVLRGDTWENV
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
YDQDPVRALDYMKQKLGYAIQPKEFFKDWEAAIHEERFNIPMISDTFGHFNQQIAFEAKD
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEECCCH
DFEGIFDGMRFDSSDLGSNAQARASVMISMIELLSAPEFDLSTGGDTVSDIYEYLLEKFA
HHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
TVLASDMGQYYTPKEISEVMARILTFGKADEDNFSIYDPAVGSASLLITTASHMKHSNQR
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEEEEEEHHHHHCCCCC
GAIKYFGQEKDATPYRLARMNLMMHNIEYNDIQIHHADTLESDWPDGVIEGKDTPRMFDA
CCEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHH
VMANPPYSAHWNNKDREDDPRFREYGIAPKTKADYSFLLHCLYHTKESGRVAIILPHGVL
HHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCEE
FRGAAEGRIRKALIDKHQIEAVIGFPDKLFLNTGIPVCVLILKKNRANSDILFVDASQGF
ECCCCHHHHHHHHHHHHHHHEEECCCHHHEECCCCCEEEEEEECCCCCCCEEEEECHHHH
EKMKNQKQLRPEDIDKITETVIHRKAVDKYSHLATLEEVIENDYNLNIPRYVDTFEEEES
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCC
IDLADIQGQIDEVDAEIAKANQTLANYFKELGVLK
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]