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The map label for this gene is miaA

Identifier: 15672608

GI number: 15672608

Start: 614999

End: 615883

Strand: Direct

Name: miaA

Synonym: L15012

Alternate gene names: 15672608

Gene position: 614999-615883 (Clockwise)

Preceding gene: 15672607

Following gene: 15672609

Centisome position: 26.0

GC content: 35.25

Gene sequence:

>885_bases
ATGAAAAATAATAAAGTTTTAGTGGTTGTCGGGCCTACAGCTGTGGGGAAAACAGCACTTGGCATTGATTTAGCAATCAA
ATTTAATGGTGAAATTATTTCTGGTGATTCTCAGCAAGTTTATCAAGGATTAGACATTGGAACCGCTAAGGTGACTATGG
CTGAACAAGCTCAAGTTCCTCATCATTTGATTGATGTACGTAAATGGACAGAAAATTTTTCGGTTCATGATTTTGTCATT
GAAGCTAATCAATTAATTAAAGAAATTCTTGACCAAGGAAAAGTGCCAATTATCGTTGGAGGAACTGGCTTATATATTCA
ATCTCTTATCGAAGGTTATCATCTTGGCGGACAGGAAAATCATGAGGAGATGATGAAACTTCGTGAAGAATTATCTCTTT
TATCTGATGAGGAATTGTTTGCAAAAGTGATTAAAATAAATCCAGATATTTCTGAGTTAAATCGTAGACGGGCGATTCGT
TTTTTGGAGCTGCAAACTTTCGGCTCTCAAGATGAAAATTTGGGTTCTGATTATGATTTTTTGCTTATTGGATTAAATGC
TGACAGAAAAGTGCTTTATGATCGAATTAATCAACGGGTTGATCAAATGATGCGTGAGGGCTTATTAGATGAAGCTAGAA
CTTTGTATGAGCAAGCACCAGAAGTTCAAGCGGCAAAAGGGATTGGTTATAAAGAATTTTTCCCTTATTTTTCAGGGGAC
ATTAGTTTGGAAGAAGCTGTTGAGTTGGTCAAAAGAAATTCAAGACGCTATGCCAAGCGACAATTGACTTGGTTTAAAAA
TAGAATGTCTGTCGAATTTGAAGATGTTTTTTCAAAAAATTATCCTGAACCAATTTTTGATAAAGTCACTCAGTTTTTGA
ACTGA

Upstream 100 bases:

>100_bases
AATTGAGCTGTCAGTAATTTTTTCTTGTCAGTGCTTGAATTTACAGGCTATTTCTGAAATAATAGAGAATGAAAATGGTG
AGAGTGAGTCATAAAAATAA

Downstream 100 bases:

>100_bases
CGCATTTTCTTAAAAATGTTATAATGAAAAAGATTGAAAAAAGGACTTTACAAGTAAATTTTATTAAAAATGGAGAGAAA
AAATGACTGATAAAAAATGG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MKNNKVLVVVGPTAVGKTALGIDLAIKFNGEIISGDSQQVYQGLDIGTAKVTMAEQAQVPHHLIDVRKWTENFSVHDFVI
EANQLIKEILDQGKVPIIVGGTGLYIQSLIEGYHLGGQENHEEMMKLREELSLLSDEELFAKVIKINPDISELNRRRAIR
FLELQTFGSQDENLGSDYDFLLIGLNADRKVLYDRINQRVDQMMREGLLDEARTLYEQAPEVQAAKGIGYKEFFPYFSGD
ISLEEAVELVKRNSRRYAKRQLTWFKNRMSVEFEDVFSKNYPEPIFDKVTQFLN

Sequences:

>Translated_294_residues
MKNNKVLVVVGPTAVGKTALGIDLAIKFNGEIISGDSQQVYQGLDIGTAKVTMAEQAQVPHHLIDVRKWTENFSVHDFVI
EANQLIKEILDQGKVPIIVGGTGLYIQSLIEGYHLGGQENHEEMMKLREELSLLSDEELFAKVIKINPDISELNRRRAIR
FLELQTFGSQDENLGSDYDFLLIGLNADRKVLYDRINQRVDQMMREGLLDEARTLYEQAPEVQAAKGIGYKEFFPYFSGD
ISLEEAVELVKRNSRRYAKRQLTWFKNRMSVEFEDVFSKNYPEPIFDKVTQFLN
>Mature_294_residues
MKNNKVLVVVGPTAVGKTALGIDLAIKFNGEIISGDSQQVYQGLDIGTAKVTMAEQAQVPHHLIDVRKWTENFSVHDFVI
EANQLIKEILDQGKVPIIVGGTGLYIQSLIEGYHLGGQENHEEMMKLREELSLLSDEELFAKVIKINPDISELNRRRAIR
FLELQTFGSQDENLGSDYDFLLIGLNADRKVLYDRINQRVDQMMREGLLDEARTLYEQAPEVQAAKGIGYKEFFPYFSGD
ISLEEAVELVKRNSRRYAKRQLTWFKNRMSVEFEDVFSKNYPEPIFDKVTQFLN

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=312, Percent_Identity=31.0897435897436, Blast_Score=134, Evalue=9e-32,
Organism=Escherichia coli, GI1790613, Length=305, Percent_Identity=30.1639344262295, Blast_Score=144, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI25144712, Length=305, Percent_Identity=31.1475409836066, Blast_Score=114, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6324848, Length=295, Percent_Identity=34.9152542372881, Blast_Score=166, Evalue=6e-42,
Organism=Drosophila melanogaster, GI24649830, Length=337, Percent_Identity=34.1246290801187, Blast_Score=145, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_LACLA (Q9CHU2)

Other databases:

- EMBL:   AE005176
- PIR:   B86703
- RefSeq:   NP_266782.1
- ProteinModelPortal:   Q9CHU2
- SMR:   Q9CHU2
- GeneID:   1114246
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L15012
- NMPDR:   fig|272623.1.peg.641
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   LLAC272623:L15012-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33624; Mature: 33624

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNKVLVVVGPTAVGKTALGIDLAIKFNGEIISGDSQQVYQGLDIGTAKVTMAEQAQVP
CCCCEEEEEECCCHHCCCEECEEEEEEECCEEECCCHHHHHHCCCCCCCEEEEHHHHCCC
HHLIDVRKWTENFSVHDFVIEANQLIKEILDQGKVPIIVGGTGLYIQSLIEGYHLGGQEN
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCCC
HEEMMKLREELSLLSDEELFAKVIKINPDISELNRRRAIRFLELQTFGSQDENLGSDYDF
HHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHEEEHHCCCCCCCCCCCCCE
LLIGLNADRKVLYDRINQRVDQMMREGLLDEARTLYEQAPEVQAAKGIGYKEFFPYFSGD
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHCCCCCCC
ISLEEAVELVKRNSRRYAKRQLTWFKNRMSVEFEDVFSKNYPEPIFDKVTQFLN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKNNKVLVVVGPTAVGKTALGIDLAIKFNGEIISGDSQQVYQGLDIGTAKVTMAEQAQVP
CCCCEEEEEECCCHHCCCEECEEEEEEECCEEECCCHHHHHHCCCCCCCEEEEHHHHCCC
HHLIDVRKWTENFSVHDFVIEANQLIKEILDQGKVPIIVGGTGLYIQSLIEGYHLGGQEN
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCCC
HEEMMKLREELSLLSDEELFAKVIKINPDISELNRRRAIRFLELQTFGSQDENLGSDYDF
HHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHEEEHHCCCCCCCCCCCCCE
LLIGLNADRKVLYDRINQRVDQMMREGLLDEARTLYEQAPEVQAAKGIGYKEFFPYFSGD
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHCCCCCCC
ISLEEAVELVKRNSRRYAKRQLTWFKNRMSVEFEDVFSKNYPEPIFDKVTQFLN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471