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The map label for this gene is yfjF

Identifier: 15672591

GI number: 15672591

Start: 598274

End: 599779

Strand: Reverse

Name: yfjF

Synonym: L198289

Alternate gene names: 15672591

Gene position: 599779-598274 (Counterclockwise)

Preceding gene: 15672596

Following gene: 30024002

Centisome position: 25.35

GC content: 38.38

Gene sequence:

>1506_bases
ATGGCAAAATCAAAAAAGAATGAAAAAGAAAAATTGCCAAAGGAATTGACGCGAAATGCTTGGATTTTAGTCGTTGGTGC
TATTGCTCCAATGCTTGATTCAACCATGGTCAATATCGCAATCAACAAACTTCAAAGTGACTTACATACTTCACTAGACA
TGATTCAGTGGGCAATTACAGGCTACGTTCTTGCACTTGCTGTAGCCGTCCCTGTCTGTGGTTATGTTGTCAATCACATT
GATGGAAAAAAAGTAATGCAGTGGTCACTCCTTAGTTTTGGACTTTTTAGCTTTTTATCTGGAATTGCTTGGAATATTGA
TAGTTTTATCATTTTCCGAGCTGTTCAAGGATTTTCAGCTGGTTTTATCACTTTACTGATGACAACCCTGCTAATGCAAA
TTACGCCTCCAGATAAACTTGGTCAATTGATGGCTGTTGTCTCAACTCCTATTATTCTTGGACCAATTATCGGGCCAGTA
CTTGGTGGTGCAATTGTATCTGGAGCAAATTGGCACTGGATTTTCTATATCAATATTCCGGTAGTAATTATTGCAGTCCT
CCTTAATCATTTTTATCTGAGTTCTTTCAAACCTTTCAACCCTAAAGCTAAACTAGATTGGTTTGGAACACTCCTTTTGG
CTGGCGGTTCTGTGGGATTAATTTACGGAATGATGAAGGGTTCAACTAATGCTAAAAATTTTTTCAATAGTCAAATGATT
ACTTTTGTCATCATTGGCTTAGCCTTGTTTGTTATTTACGGAATTTATAATCGCATTCGCAAAAATAATACTGTTCTTCC
GCTCAGATTTTTCAAATCTAAAAACTTCACTGCAGCGAATATTGGAATCTTTTTAGCTGGAATTGCTTCTAATGGACCTA
TGCTTTTACTGCCACTTTTCTTTCAAAATACAAAAGGCTTTACAGCCGTTGAAGCAGGTCTAATGTTGATTCCCCAAGGG
CTTGGTATGATGGTGACTCGACCTTTGATTGGGAAGATGATTGACCGTATGGGAGCTCGTCCTGTAGTACTTGTTTCCCT
GGCTATTTCCCTTCTTGGAACACTTCCTTTCATTTTTGTTCACGAAAATACAAATTTGATTTGGTTGGGATTGGTTTTAT
TTGTTCGTGGAATGGGAATTGGTGGTTTGCAAATGCCAATGATGACTGATATTTTCATTGGTTTAGACAAACAAGATATC
CCTGGTGCATCAGTAGGACAAAGAATCATTCAAAACGTTGGTTCATCTTTTGGTTCAGCCGTCATCTCGGCAGTTGTGAC
GGCGGTTGTGACGTCTGAAATTGCTGGACAGATGACAACTAAACTACATCAATTAGCGGCGATGCACACCAATTTAACAC
CGACTGTCATTGCAAAAGCAAAAACTTCTGTTGAAAAAGCGGTCACGCTTAATGGTTATCAACTTGGCTTTCTTGTCTCT
TCGATTGTTTTAATTGTGATTGCTATTCCAGCCATGTTCCTTTCACAAAAGAAGGTCAACAAATAA

Upstream 100 bases:

>100_bases
AATAATTTTGTATTTAAGATACAAAATGTATGTTATTTTTAAATTATAATTCCTAAGTTTTGATAACTTAGTTTTCGCTC
AGACGAAAGAAAGGATTAAC

Downstream 100 bases:

>100_bases
AATTCGTAGTTTTTAGCTTTGATTTCACTTGTTTTTTGATATAATTGAATTGAATTATCTCAATAATAGATAGATGAACT
TTAATCAATTAACTGAGCTT

Product: membrane-bound transport protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MAKSKKNEKEKLPKELTRNAWILVVGAIAPMLDSTMVNIAINKLQSDLHTSLDMIQWAITGYVLALAVAVPVCGYVVNHI
DGKKVMQWSLLSFGLFSFLSGIAWNIDSFIIFRAVQGFSAGFITLLMTTLLMQITPPDKLGQLMAVVSTPIILGPIIGPV
LGGAIVSGANWHWIFYINIPVVIIAVLLNHFYLSSFKPFNPKAKLDWFGTLLLAGGSVGLIYGMMKGSTNAKNFFNSQMI
TFVIIGLALFVIYGIYNRIRKNNTVLPLRFFKSKNFTAANIGIFLAGIASNGPMLLLPLFFQNTKGFTAVEAGLMLIPQG
LGMMVTRPLIGKMIDRMGARPVVLVSLAISLLGTLPFIFVHENTNLIWLGLVLFVRGMGIGGLQMPMMTDIFIGLDKQDI
PGASVGQRIIQNVGSSFGSAVISAVVTAVVTSEIAGQMTTKLHQLAAMHTNLTPTVIAKAKTSVEKAVTLNGYQLGFLVS
SIVLIVIAIPAMFLSQKKVNK

Sequences:

>Translated_501_residues
MAKSKKNEKEKLPKELTRNAWILVVGAIAPMLDSTMVNIAINKLQSDLHTSLDMIQWAITGYVLALAVAVPVCGYVVNHI
DGKKVMQWSLLSFGLFSFLSGIAWNIDSFIIFRAVQGFSAGFITLLMTTLLMQITPPDKLGQLMAVVSTPIILGPIIGPV
LGGAIVSGANWHWIFYINIPVVIIAVLLNHFYLSSFKPFNPKAKLDWFGTLLLAGGSVGLIYGMMKGSTNAKNFFNSQMI
TFVIIGLALFVIYGIYNRIRKNNTVLPLRFFKSKNFTAANIGIFLAGIASNGPMLLLPLFFQNTKGFTAVEAGLMLIPQG
LGMMVTRPLIGKMIDRMGARPVVLVSLAISLLGTLPFIFVHENTNLIWLGLVLFVRGMGIGGLQMPMMTDIFIGLDKQDI
PGASVGQRIIQNVGSSFGSAVISAVVTAVVTSEIAGQMTTKLHQLAAMHTNLTPTVIAKAKTSVEKAVTLNGYQLGFLVS
SIVLIVIAIPAMFLSQKKVNK
>Mature_500_residues
AKSKKNEKEKLPKELTRNAWILVVGAIAPMLDSTMVNIAINKLQSDLHTSLDMIQWAITGYVLALAVAVPVCGYVVNHID
GKKVMQWSLLSFGLFSFLSGIAWNIDSFIIFRAVQGFSAGFITLLMTTLLMQITPPDKLGQLMAVVSTPIILGPIIGPVL
GGAIVSGANWHWIFYINIPVVIIAVLLNHFYLSSFKPFNPKAKLDWFGTLLLAGGSVGLIYGMMKGSTNAKNFFNSQMIT
FVIIGLALFVIYGIYNRIRKNNTVLPLRFFKSKNFTAANIGIFLAGIASNGPMLLLPLFFQNTKGFTAVEAGLMLIPQGL
GMMVTRPLIGKMIDRMGARPVVLVSLAISLLGTLPFIFVHENTNLIWLGLVLFVRGMGIGGLQMPMMTDIFIGLDKQDIP
GASVGQRIIQNVGSSFGSAVISAVVTAVVTSEIAGQMTTKLHQLAAMHTNLTPTVIAKAKTSVEKAVTLNGYQLGFLVSS
IVLIVIAIPAMFLSQKKVNK

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=410, Percent_Identity=27.5609756097561, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1788710, Length=395, Percent_Identity=30.8860759493671, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1790195, Length=434, Percent_Identity=26.2672811059908, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI87081983, Length=426, Percent_Identity=25.3521126760563, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788392, Length=372, Percent_Identity=29.3010752688172, Blast_Score=109, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6319770, Length=466, Percent_Identity=22.5321888412017, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6322958, Length=429, Percent_Identity=22.8438228438228, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323735, Length=307, Percent_Identity=20.8469055374593, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6321663, Length=131, Percent_Identity=29.0076335877863, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 54234; Mature: 54103

Theoretical pI: Translated: 10.65; Mature: 10.65

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKSKKNEKEKLPKELTRNAWILVVGAIAPMLDSTMVNIAINKLQSDLHTSLDMIQWAIT
CCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYVLALAVAVPVCGYVVNHIDGKKVMQWSLLSFGLFSFLSGIAWNIDSFIIFRAVQGFSA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCH
GFITLLMTTLLMQITPPDKLGQLMAVVSTPIILGPIIGPVLGGAIVSGANWHWIFYINIP
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEECHH
VVIIAVLLNHFYLSSFKPFNPKAKLDWFGTLLLAGGSVGLIYGMMKGSTNAKNFFNSQMI
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHH
TFVIIGLALFVIYGIYNRIRKNNTVLPLRFFKSKNFTAANIGIFLAGIASNGPMLLLPLF
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEHHEEEEEECCCCCEEEEEEE
FQNTKGFTAVEAGLMLIPQGLGMMVTRPLIGKMIDRMGARPVVLVSLAISLLGTLPFIFV
CCCCCCCHHHHHCHHEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEE
HENTNLIWLGLVLFVRGMGIGGLQMPMMTDIFIGLDKQDIPGASVGQRIIQNVGSSFGSA
ECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VISAVVTAVVTSEIAGQMTTKLHQLAAMHTNLTPTVIAKAKTSVEKAVTLNGYQLGFLVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCHHHHHHH
SIVLIVIAIPAMFLSQKKVNK
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]