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The map label for this gene is ldhB

Identifier: 15672358

GI number: 15672358

Start: 379657

End: 380601

Strand: Reverse

Name: ldhB

Synonym: L0018

Alternate gene names: 15672358

Gene position: 380601-379657 (Counterclockwise)

Preceding gene: 15672359

Following gene: 15672356

Centisome position: 16.09

GC content: 35.13

Gene sequence:

>945_bases
ATGAAAATTACAAGCAGAAAAGTAGTTGTTATAGGAACAGGTTTTGTTGGAACAAGTATTGCTTATTCAATGATTAATCA
AGGACTTGTTAATGAGTTGGTTTTGATAGATGTTAATCAGGATAAAGCTGAGGGTGAAGCCTTAGATTTATTAGATGGCG
TTTCTTGGGGGCAAGAAAATGTTATTGTGAGAGCTGGAGATTATAAGGATTGTAAAAACGCTGATATTGTGGTCGTTACG
GCTGGGGTAAATCAAAAACCAGGTCAATCACGTTTAGATTTGGTTAATACAAATGCTAAAATTATGCGCTCAATCGTGAC
TCAGGTTATGGACTCAGGTTTTGATGGTATTTTTGTAATTGCTTCAAATCCTGTGGATATTCTTACCTATGTCGCTTGGG
AAACTTCTGGTCTTGATCAATCTCGAATTGTCGGAACTGGTACAACTTTGGATACTACTCGTTTTAGAAAAGAATTGGCC
ACAAAGCTAGAAATTGATCCACGGAGCGTTCATGGATATATTATTGGCGAACATGGCGATTCTGAAGTTGCAGTTTGGTC
ACACACGACGGTAGGTGGAAAACCTATTCTTGAATTTATTGTTAAAAATAAAAAAATAGGGGTTGAAGATTTGTCTAATT
TGTCAAATAAGGTAAAAAATGCTGCCTATGAGATTATTGATAAAAAACAGGCTACTTATTACGGCATTGGAATGAGTACA
GCTAGAATTGTCAAGGCCATCTTAAATAATGAACAAGCAATTCTACCAGTTTCTGCCTATTTACGTGGTGAATATGGTCA
AGAAGGTGTCTTTACCGGTGTTCCATCTATTGTAAATCAAAATGGTGTTAGAGAAATCATTGAACTAAATATTGATGCTT
ATGAAAAGAAACAATTTGAAAAATCTGTCAGTCAACTTAAAGAGGTAATAGAATCTATTAAGTAA

Upstream 100 bases:

>100_bases
TTTCTATTATTTTAATTTTATGTACTATGATTAGTTATCTTTGGCTTTTTAACAGATAGGCTTTATAAAAAAATATATAT
AAATTGAAAAGGAGCTAAAA

Downstream 100 bases:

>100_bases
TCTATCCAGACCTTACAAATATAAAGGAAAATAAAATGCTTGCTCTACTCTTTTTAGTCATTGCTTCACTTTGGGGATTA
ATCTGGTTTAACATTAATGA

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH 2

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKITSRKVVVIGTGFVGTSIAYSMINQGLVNELVLIDVNQDKAEGEALDLLDGVSWGQENVIVRAGDYKDCKNADIVVVT
AGVNQKPGQSRLDLVNTNAKIMRSIVTQVMDSGFDGIFVIASNPVDILTYVAWETSGLDQSRIVGTGTTLDTTRFRKELA
TKLEIDPRSVHGYIIGEHGDSEVAVWSHTTVGGKPILEFIVKNKKIGVEDLSNLSNKVKNAAYEIIDKKQATYYGIGMST
ARIVKAILNNEQAILPVSAYLRGEYGQEGVFTGVPSIVNQNGVREIIELNIDAYEKKQFEKSVSQLKEVIESIK

Sequences:

>Translated_314_residues
MKITSRKVVVIGTGFVGTSIAYSMINQGLVNELVLIDVNQDKAEGEALDLLDGVSWGQENVIVRAGDYKDCKNADIVVVT
AGVNQKPGQSRLDLVNTNAKIMRSIVTQVMDSGFDGIFVIASNPVDILTYVAWETSGLDQSRIVGTGTTLDTTRFRKELA
TKLEIDPRSVHGYIIGEHGDSEVAVWSHTTVGGKPILEFIVKNKKIGVEDLSNLSNKVKNAAYEIIDKKQATYYGIGMST
ARIVKAILNNEQAILPVSAYLRGEYGQEGVFTGVPSIVNQNGVREIIELNIDAYEKKQFEKSVSQLKEVIESIK
>Mature_314_residues
MKITSRKVVVIGTGFVGTSIAYSMINQGLVNELVLIDVNQDKAEGEALDLLDGVSWGQENVIVRAGDYKDCKNADIVVVT
AGVNQKPGQSRLDLVNTNAKIMRSIVTQVMDSGFDGIFVIASNPVDILTYVAWETSGLDQSRIVGTGTTLDTTRFRKELA
TKLEIDPRSVHGYIIGEHGDSEVAVWSHTTVGGKPILEFIVKNKKIGVEDLSNLSNKVKNAAYEIIDKKQATYYGIGMST
ARIVKAILNNEQAILPVSAYLRGEYGQEGVFTGVPSIVNQNGVREIIELNIDAYEKKQFEKSVSQLKEVIESIK

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family

Homologues:

Organism=Homo sapiens, GI291575128, Length=317, Percent_Identity=40.6940063091483, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI4557032, Length=317, Percent_Identity=40.6940063091483, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI15082234, Length=313, Percent_Identity=39.6166134185303, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI9257228, Length=305, Percent_Identity=39.344262295082, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI4504973, Length=305, Percent_Identity=39.344262295082, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI47059044, Length=317, Percent_Identity=37.8548895899054, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI221136809, Length=317, Percent_Identity=37.8548895899054, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI5031857, Length=305, Percent_Identity=37.3770491803279, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI260099723, Length=305, Percent_Identity=37.3770491803279, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI260099727, Length=187, Percent_Identity=43.3155080213904, Blast_Score=168, Evalue=6e-42,
Organism=Homo sapiens, GI260099725, Length=187, Percent_Identity=43.3155080213904, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI207028494, Length=208, Percent_Identity=35.5769230769231, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI103472011, Length=291, Percent_Identity=27.1477663230241, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI103472015, Length=184, Percent_Identity=27.7173913043478, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI21735621, Length=319, Percent_Identity=24.4514106583072, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1789632, Length=252, Percent_Identity=30.1587301587302, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17535107, Length=298, Percent_Identity=37.5838926174497, Blast_Score=208, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17554310, Length=325, Percent_Identity=26.1538461538462, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17136226, Length=316, Percent_Identity=38.6075949367089, Blast_Score=233, Evalue=1e-61,
Organism=Drosophila melanogaster, GI45550422, Length=313, Percent_Identity=31.629392971246, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24647881, Length=320, Percent_Identity=28.125, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24663599, Length=315, Percent_Identity=24.1269841269841, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24663595, Length=304, Percent_Identity=25.6578947368421, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): LDH2_LACLA (Q9CII4)

Other databases:

- EMBL:   AE005176
- PIR:   H86671
- RefSeq:   NP_266532.1
- ProteinModelPortal:   Q9CII4
- SMR:   Q9CII4
- GeneID:   1113987
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0018
- NMPDR:   fig|272623.1.peg.384
- HOGENOM:   HBG566126
- OMA:   GMSTARI
- ProtClustDB:   CLSK697192
- BioCyc:   LLAC272623:L0018-MONOMER
- BRENDA:   1.1.1.27
- GO:   GO:0005737
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00488
- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01771

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.27

Molecular weight: Translated: 34312; Mature: 34312

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00064 L_LDH

Important sites: ACT_SITE 178-178 BINDING 91-91 BINDING 123-123 BINDING 154-154 BINDING 232-232

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITSRKVVVIGTGFVGTSIAYSMINQGLVNELVLIDVNQDKAEGEALDLLDGVSWGQEN
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHCCCCCCCC
VIVRAGDYKDCKNADIVVVTAGVNQKPGQSRLDLVNTNAKIMRSIVTQVMDSGFDGIFVI
EEEEECCCCCCCCCCEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEE
ASNPVDILTYVAWETSGLDQSRIVGTGTTLDTTRFRKELATKLEIDPRSVHGYIIGEHGD
ECCCHHEEEEEEEECCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCC
SEVAVWSHTTVGGKPILEFIVKNKKIGVEDLSNLSNKVKNAAYEIIDKKQATYYGIGMST
CEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHH
ARIVKAILNNEQAILPVSAYLRGEYGQEGVFTGVPSIVNQNGVREIIELNIDAYEKKQFE
HHHHHHHHCCCCEEEEHHHHHCCCCCCCCCCCCCCHHHCCCHHHHHHHCCCCHHHHHHHH
KSVSQLKEVIESIK
HHHHHHHHHHHHCC
>Mature Secondary Structure
MKITSRKVVVIGTGFVGTSIAYSMINQGLVNELVLIDVNQDKAEGEALDLLDGVSWGQEN
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHCCCCCCCC
VIVRAGDYKDCKNADIVVVTAGVNQKPGQSRLDLVNTNAKIMRSIVTQVMDSGFDGIFVI
EEEEECCCCCCCCCCEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEE
ASNPVDILTYVAWETSGLDQSRIVGTGTTLDTTRFRKELATKLEIDPRSVHGYIIGEHGD
ECCCHHEEEEEEEECCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCC
SEVAVWSHTTVGGKPILEFIVKNKKIGVEDLSNLSNKVKNAAYEIIDKKQATYYGIGMST
CEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHH
ARIVKAILNNEQAILPVSAYLRGEYGQEGVFTGVPSIVNQNGVREIIELNIDAYEKKQFE
HHHHHHHHCCCCEEEEHHHHHCCCCCCCCCCCCCCHHHCCCHHHHHHHCCCCHHHHHHHH
KSVSQLKEVIESIK
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471