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The map label for this gene is prfC

Identifier: 15672331

GI number: 15672331

Start: 351198

End: 352769

Strand: Direct

Name: prfC

Synonym: L0369

Alternate gene names: 15672331

Gene position: 351198-352769 (Clockwise)

Preceding gene: 15672330

Following gene: 15672332

Centisome position: 14.85

GC content: 38.61

Gene sequence:

>1572_bases
ATGACTTTACAAGAAGAAATAAAAAAACGCCGGACTTTTGCCATCATCAGTCACCCGGATGCTGGTAAGACGACGATTAC
AGAACAATTATTGAAATTTGGGGGAGCCATTCGAGAAGCGGGGACTGTAAAAGCTCGTAAAACTGGTAATTTTGCTAAGT
CAGACTGGATGGATATTGAAAAAGAACGTGGAATTTCTGTAACAAGTTCTGTCATGCAATTTGATTATGCAGGCAAGCGT
GTTAATATCTTGGACACGCCTGGGCACGAAGATTTCTCAGAAGATACTTACCGAACATTGATGGCAGTTGATGCTGCGGT
CATGGTTATTGACAGTGCTAAAGGGATTGAAGCACAAACGAAAAAACTTTTCCAAGTTGTAAAACGCAGAGGGATTCCGG
TTTTCACTTTCATTAATAAACTTGACCGTGATGGACGTGAACCGCTTGATTTACTTTCTGAATTGGAAGAAATTCTTGGG
ATTGCTAGTGTACCAATGAATTGGCCAATCGGAATGGGGAAAAATTTCCAAGGTCTTTATGATTTTACGCATGGTCGTGT
GGAAGTTTATCAACCTGAGGATGGCAAACGTTTTGTTGAATTTGATGAAAATGGGGAAGTGCCGGCTAGTCATCCTTTGA
CAAAAAATCCATTCTTTACTCAAGCTTTAGAAGATGCTGAATTACTTTTGGATGCAGGGAATCAATTTTCTGAGGAAGAA
GTTGTTGCAGGGCAATTGACACCAGTTTTCTTTGGTTCGGCTTTAACTAGTTTTGGGGTAGAAACTTTCCTTGAAACATT
TTTAGAATATGCCCCAGAGCCTCATTCACACAAAACGGTGGACGAAGAAGAAATTGAACCTTTAAATCCTGATTTTTCTG
GTTTCATTTTCAAAATCCAAGCAAATATGGACCCACGTCACCGTGACCGAATTGCTTTTGTTCGGATTGTATCTGGTGAA
TTTGAACGAGGAATGGATGTTAATCTTATTCGGACGGGTAAGAAAGTGAAACTCTCAAATGTCACACAATTTATGGCTGA
ATCGCGAGAAAATGTTGAGAATGCTGTAGCTGGTGATATTATTGGGGTTTATGATACTGGAACTTATCAGGTTGGGGATA
CATTAACCACAGGTAAATTGAAAAAATCTTTTGAGCCATTGCCAACATTTACCCCAGAGCTCTTTATGCGTGTCCAAGCG
AAAAATGTGATGAAACAAAAATCTTTCCAAAAAGGAATTGACCAATTGGTACAAGAAGGAGCCATTCAGCTCTACAAATC
TTATACAACTGGTGATATTATGCTTGGTGCGGTTGGGCAACTTCAATTTGAAGTCTTTAAAGACCGGATGGAGCGTGAAT
ATAATTCTGAAACAATCATGACACCAATGGGGACAAAAACGGTTCGTTGGATTAAAGAAGAAGATTTAGATGAAAAAATG
TCTAGTTCACGTAATATTTTGGCACGAGATCGTTTTGACCATCCTTTATTCTTGTTTGAAAATGAATTTGCCATGCGTTG
GTTTAAAGATAAATATCCAGATGTTGAATTAATGGAACAATTTTCAGTATAA

Upstream 100 bases:

>100_bases
AAATTTTGATATAATTAAAAGAACTATTTTTTTTTGAGGATTAACAAATGTAAAATAAATTAAAAATATAAATGAATAAT
TAAAGAAAAAGAGAGTATAA

Downstream 100 bases:

>100_bases
AATTACTGACAGATATTTTATATAGTAGAAAAGCTTATATTCTTTAAAAAATATCATAAAAATTTACTGACAGCTTTGTC
AGTATATTTTTTATGAAAAT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MTLQEEIKKRRTFAIISHPDAGKTTITEQLLKFGGAIREAGTVKARKTGNFAKSDWMDIEKERGISVTSSVMQFDYAGKR
VNILDTPGHEDFSEDTYRTLMAVDAAVMVIDSAKGIEAQTKKLFQVVKRRGIPVFTFINKLDRDGREPLDLLSELEEILG
IASVPMNWPIGMGKNFQGLYDFTHGRVEVYQPEDGKRFVEFDENGEVPASHPLTKNPFFTQALEDAELLLDAGNQFSEEE
VVAGQLTPVFFGSALTSFGVETFLETFLEYAPEPHSHKTVDEEEIEPLNPDFSGFIFKIQANMDPRHRDRIAFVRIVSGE
FERGMDVNLIRTGKKVKLSNVTQFMAESRENVENAVAGDIIGVYDTGTYQVGDTLTTGKLKKSFEPLPTFTPELFMRVQA
KNVMKQKSFQKGIDQLVQEGAIQLYKSYTTGDIMLGAVGQLQFEVFKDRMEREYNSETIMTPMGTKTVRWIKEEDLDEKM
SSSRNILARDRFDHPLFLFENEFAMRWFKDKYPDVELMEQFSV

Sequences:

>Translated_523_residues
MTLQEEIKKRRTFAIISHPDAGKTTITEQLLKFGGAIREAGTVKARKTGNFAKSDWMDIEKERGISVTSSVMQFDYAGKR
VNILDTPGHEDFSEDTYRTLMAVDAAVMVIDSAKGIEAQTKKLFQVVKRRGIPVFTFINKLDRDGREPLDLLSELEEILG
IASVPMNWPIGMGKNFQGLYDFTHGRVEVYQPEDGKRFVEFDENGEVPASHPLTKNPFFTQALEDAELLLDAGNQFSEEE
VVAGQLTPVFFGSALTSFGVETFLETFLEYAPEPHSHKTVDEEEIEPLNPDFSGFIFKIQANMDPRHRDRIAFVRIVSGE
FERGMDVNLIRTGKKVKLSNVTQFMAESRENVENAVAGDIIGVYDTGTYQVGDTLTTGKLKKSFEPLPTFTPELFMRVQA
KNVMKQKSFQKGIDQLVQEGAIQLYKSYTTGDIMLGAVGQLQFEVFKDRMEREYNSETIMTPMGTKTVRWIKEEDLDEKM
SSSRNILARDRFDHPLFLFENEFAMRWFKDKYPDVELMEQFSV
>Mature_522_residues
TLQEEIKKRRTFAIISHPDAGKTTITEQLLKFGGAIREAGTVKARKTGNFAKSDWMDIEKERGISVTSSVMQFDYAGKRV
NILDTPGHEDFSEDTYRTLMAVDAAVMVIDSAKGIEAQTKKLFQVVKRRGIPVFTFINKLDRDGREPLDLLSELEEILGI
ASVPMNWPIGMGKNFQGLYDFTHGRVEVYQPEDGKRFVEFDENGEVPASHPLTKNPFFTQALEDAELLLDAGNQFSEEEV
VAGQLTPVFFGSALTSFGVETFLETFLEYAPEPHSHKTVDEEEIEPLNPDFSGFIFKIQANMDPRHRDRIAFVRIVSGEF
ERGMDVNLIRTGKKVKLSNVTQFMAESRENVENAVAGDIIGVYDTGTYQVGDTLTTGKLKKSFEPLPTFTPELFMRVQAK
NVMKQKSFQKGIDQLVQEGAIQLYKSYTTGDIMLGAVGQLQFEVFKDRMEREYNSETIMTPMGTKTVRWIKEEDLDEKMS
SSRNILARDRFDHPLFLFENEFAMRWFKDKYPDVELMEQFSV

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily

Homologues:

Organism=Homo sapiens, GI18390331, Length=488, Percent_Identity=29.9180327868852, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI19923640, Length=515, Percent_Identity=27.378640776699, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI25306283, Length=398, Percent_Identity=29.6482412060301, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI25306287, Length=294, Percent_Identity=32.312925170068, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI157426893, Length=157, Percent_Identity=32.484076433121, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI94966754, Length=167, Percent_Identity=29.3413173652695, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI4503483, Length=150, Percent_Identity=30, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1790835, Length=530, Percent_Identity=46.9811320754717, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI1789738, Length=489, Percent_Identity=28.0163599182004, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1788922, Length=160, Percent_Identity=31.875, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI48994988, Length=181, Percent_Identity=32.0441988950276, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789737, Length=144, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=144, Percent_Identity=27.7777777777778, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17533571, Length=479, Percent_Identity=27.9749478079332, Blast_Score=192, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17556745, Length=478, Percent_Identity=25.7322175732218, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17557151, Length=150, Percent_Identity=30.6666666666667, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=32.3308270676692, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=32.3308270676692, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17506493, Length=171, Percent_Identity=28.6549707602339, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=483, Percent_Identity=27.536231884058, Blast_Score=194, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6322359, Length=510, Percent_Identity=25.2941176470588, Blast_Score=147, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6323320, Length=138, Percent_Identity=31.8840579710145, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6324707, Length=159, Percent_Identity=30.188679245283, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=159, Percent_Identity=30.188679245283, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=199, Percent_Identity=27.6381909547739, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24582462, Length=482, Percent_Identity=28.2157676348548, Blast_Score=196, Evalue=3e-50,
Organism=Drosophila melanogaster, GI221458488, Length=506, Percent_Identity=25.4940711462451, Blast_Score=148, Evalue=8e-36,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=33.9622641509434, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24585711, Length=159, Percent_Identity=29.559748427673, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24585713, Length=159, Percent_Identity=29.559748427673, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24585709, Length=159, Percent_Identity=29.559748427673, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28574573, Length=170, Percent_Identity=31.1764705882353, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF3_LACLA (Q9CIK7)

Other databases:

- EMBL:   AE005176
- PIR:   E86668
- RefSeq:   NP_266505.1
- ProteinModelPortal:   Q9CIK7
- SMR:   Q9CIK7
- GeneID:   1113960
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0369
- NMPDR:   fig|272623.1.peg.357
- HOGENOM:   HBG285678
- OMA:   LQFEVVQ
- ProtClustDB:   PRK00741
- BioCyc:   LLAC272623:L0369-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00072
- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00503
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 59503; Mature: 59372

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQEEIKKRRTFAIISHPDAGKTTITEQLLKFGGAIREAGTVKARKTGNFAKSDWMDIE
CCCHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCHHHHCCCEEECCCCCCCHHHCCCCH
KERGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMAVDAAVMVIDSAKGIEAQT
HHCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHEEEECCCCCHHHH
KKLFQVVKRRGIPVFTFINKLDRDGREPLDLLSELEEILGIASVPMNWPIGMGKNFQGLY
HHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
DFTHGRVEVYQPEDGKRFVEFDENGEVPASHPLTKNPFFTQALEDAELLLDAGNQFSEEE
CCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCH
VVAGQLTPVFFGSALTSFGVETFLETFLEYAPEPHSHKTVDEEEIEPLNPDFSGFIFKIQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCEEEEEE
ANMDPRHRDRIAFVRIVSGEFERGMDVNLIRTGKKVKLSNVTQFMAESRENVENAVAGDI
CCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHCCE
IGVYDTGTYQVGDTLTTGKLKKSFEPLPTFTPELFMRVQAKNVMKQKSFQKGIDQLVQEG
EEEEECCCEECCCCEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIQLYKSYTTGDIMLGAVGQLQFEVFKDRMEREYNSETIMTPMGTKTVRWIKEEDLDEKM
HHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHH
SSSRNILARDRFDHPLFLFENEFAMRWFKDKYPDVELMEQFSV
HHCCCEEECCCCCCCEEEECCHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure 
TLQEEIKKRRTFAIISHPDAGKTTITEQLLKFGGAIREAGTVKARKTGNFAKSDWMDIE
CCHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCHHHHCCCEEECCCCCCCHHHCCCCH
KERGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMAVDAAVMVIDSAKGIEAQT
HHCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHEEEECCCCCHHHH
KKLFQVVKRRGIPVFTFINKLDRDGREPLDLLSELEEILGIASVPMNWPIGMGKNFQGLY
HHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
DFTHGRVEVYQPEDGKRFVEFDENGEVPASHPLTKNPFFTQALEDAELLLDAGNQFSEEE
CCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCH
VVAGQLTPVFFGSALTSFGVETFLETFLEYAPEPHSHKTVDEEEIEPLNPDFSGFIFKIQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCEEEEEE
ANMDPRHRDRIAFVRIVSGEFERGMDVNLIRTGKKVKLSNVTQFMAESRENVENAVAGDI
CCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHCCE
IGVYDTGTYQVGDTLTTGKLKKSFEPLPTFTPELFMRVQAKNVMKQKSFQKGIDQLVQEG
EEEEECCCEECCCCEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIQLYKSYTTGDIMLGAVGQLQFEVFKDRMEREYNSETIMTPMGTKTVRWIKEEDLDEKM
HHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHH
SSSRNILARDRFDHPLFLFENEFAMRWFKDKYPDVELMEQFSV
HHCCCEEECCCCCCCEEEECCHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471