Click here to switch to the map view.

The map label for this gene is tgt

Identifier: 15672134

GI number: 15672134

Start: 155375

End: 156523

Strand: Reverse

Name: tgt

Synonym: L0361

Alternate gene names: 15672134

Gene position: 156523-155375 (Counterclockwise)

Preceding gene: 15672136

Following gene: 15672132

Centisome position: 6.62

GC content: 42.47

Gene sequence:

>1149_bases
ATGACAGAACACGCAATAAAATACAGATTAATAAAAAAAGAAAAACATACAGGAGCAAGATTAGGAGAAATCATCACACC
ACATGGTACCTTCCCAACTCCAATGTTTATGCCCGTAGGTACTCAAGCTACCGTTAAAACAATGAGTCCTGAAGAACTCA
AAACATTAGGCTCAGGGATTATTCTCTCAAACACTTACCACCTCTGGTTACGCCCCGGAGATGAACTTGTTGCTGAAGCT
GGCGGTCTTCACAAATTTATGAATTGGGACCAACCTATCCTGACTGATAGTGGTGGTTTCCAGGTTTACAGCCTAGTTCA
AAACAAAAAAAATATCACAGAAGAAGGCGTTAAGTTCAAGAGCCATTTGGACGGACGTGAACTTTTTCTTAACCCCGAAA
AAGCAATAAGCATTCAAAATAATCTTGGCTCGGACATCATGATGTCCTTTGACGAATGTCCACCTTTCTATCAACCCTAT
GACTATGTCAAAGCCTCTGTTGAAAGAACTTCCCGTTGGGCTGAGCGCGGTCTCAATGCTCATCGTCGTCCAAATGACCA
AGGACTTTTCGGAATTGTTCAAGGAGCTGGTTTTGAAGATTTACGTCGTCAATCAGCTAGAGACTTGACTTCAATGGATT
TCGCTGGTTATTCTATCGGTGGTCTTGCCGTTGGTGAATCTCATAAAGAAATGAATGCTGTCCTTGACTTTACAACACCA
ATGCTTCCAGAAGACAAACCCCGCTATTTAATGGGTGTTGGTGCACCAGACAGCTTGATTGATGGAGTTATCCGTGGAGT
TGATATGTTTGACTGCGTTCTTCCTACACGAATCGCTCGTAATGGAACTCTAATGACTCATTTCGGTCGAGTTAACATTC
GTAATGCTAAATACGAACACGATTTTACTCCACTTGACCCAATGTGCGATTGCTACACATGCACTAACTATACACGCGCC
TACCTTCGTCATTTAATCAAAGCCGATGAAACTTTTGGCCTTCGTCTTTGTTCATACCACAATCTTCACTTCCTCGTAAA
TCTAATGAAAGACGTTCGCGGGGCCATTATGGATGACAACCTTCTGGAATTCCGAGAAGATTTCTGTGAGCGTTACGGCT
ACAATCAACCCAATGCCAAAGACTTCTAG

Upstream 100 bases:

>100_bases
AAAATCATCTATAAAAAGCGTTTAATTTTTGTTATACTTATAAGTGCTTGCTTTTTAAGCTACAAGAAGACTCGATTCTA
CGAGTAAATAAAGGAATCTT

Downstream 100 bases:

>100_bases
TTCTTGAAAATAGGTTTAAAAAGTTGTATCATAATAAAAGTGGGTAACCACTTTCATTTTGTGCCGAGTTAGCTCAGTCG
GTAGAGCACTTCACTCGTAA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MTEHAIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQATVKTMSPEELKTLGSGIILSNTYHLWLRPGDELVAEA
GGLHKFMNWDQPILTDSGGFQVYSLVQNKKNITEEGVKFKSHLDGRELFLNPEKAISIQNNLGSDIMMSFDECPPFYQPY
DYVKASVERTSRWAERGLNAHRRPNDQGLFGIVQGAGFEDLRRQSARDLTSMDFAGYSIGGLAVGESHKEMNAVLDFTTP
MLPEDKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIARNGTLMTHFGRVNIRNAKYEHDFTPLDPMCDCYTCTNYTRA
YLRHLIKADETFGLRLCSYHNLHFLVNLMKDVRGAIMDDNLLEFREDFCERYGYNQPNAKDF

Sequences:

>Translated_382_residues
MTEHAIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQATVKTMSPEELKTLGSGIILSNTYHLWLRPGDELVAEA
GGLHKFMNWDQPILTDSGGFQVYSLVQNKKNITEEGVKFKSHLDGRELFLNPEKAISIQNNLGSDIMMSFDECPPFYQPY
DYVKASVERTSRWAERGLNAHRRPNDQGLFGIVQGAGFEDLRRQSARDLTSMDFAGYSIGGLAVGESHKEMNAVLDFTTP
MLPEDKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIARNGTLMTHFGRVNIRNAKYEHDFTPLDPMCDCYTCTNYTRA
YLRHLIKADETFGLRLCSYHNLHFLVNLMKDVRGAIMDDNLLEFREDFCERYGYNQPNAKDF
>Mature_381_residues
TEHAIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQATVKTMSPEELKTLGSGIILSNTYHLWLRPGDELVAEAG
GLHKFMNWDQPILTDSGGFQVYSLVQNKKNITEEGVKFKSHLDGRELFLNPEKAISIQNNLGSDIMMSFDECPPFYQPYD
YVKASVERTSRWAERGLNAHRRPNDQGLFGIVQGAGFEDLRRQSARDLTSMDFAGYSIGGLAVGESHKEMNAVLDFTTPM
LPEDKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIARNGTLMTHFGRVNIRNAKYEHDFTPLDPMCDCYTCTNYTRAY
LRHLIKADETFGLRLCSYHNLHFLVNLMKDVRGAIMDDNLLEFREDFCERYGYNQPNAKDF

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=360, Percent_Identity=46.3888888888889, Blast_Score=337, Evalue=1e-92,
Organism=Escherichia coli, GI1786607, Length=360, Percent_Identity=52.7777777777778, Blast_Score=384, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17544680, Length=355, Percent_Identity=44.7887323943662, Blast_Score=293, Evalue=8e-80,
Organism=Caenorhabditis elegans, GI25146502, Length=209, Percent_Identity=30.1435406698565, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19920510, Length=372, Percent_Identity=44.0860215053763, Blast_Score=316, Evalue=1e-86,
Organism=Drosophila melanogaster, GI21355311, Length=232, Percent_Identity=27.1551724137931, Blast_Score=85, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_LACLA (Q9CJ54)

Other databases:

- EMBL:   AE005176
- PIR:   H86643
- RefSeq:   NP_266308.1
- ProteinModelPortal:   Q9CJ54
- SMR:   Q9CJ54
- GeneID:   1113760
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0361
- NMPDR:   fig|272623.1.peg.159
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- ProtClustDB:   PRK00112
- BioCyc:   LLAC272623:L0361-MONOMER
- BRENDA:   2.4.2.29
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 43343; Mature: 43212

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: ACT_SITE 96-96 BINDING 97-97

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHAIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQATVKTMSPEELKTLGSGI
CCCHHHHHHHHHHHHCCCHHHCCEECCCCCCCCCEEEECCCCCEEECCCHHHHHHHCCCE
ILSNTYHLWLRPGDELVAEAGGLHKFMNWDQPILTDSGGFQVYSLVQNKKNITEEGVKFK
EEECEEEEEECCCHHHHHHHCCHHHHHCCCCCEEECCCCCEEHHHHHHHHCHHHHHHHHH
SHLDGRELFLNPEKAISIQNNLGSDIMMSFDECPPFYQPYDYVKASVERTSRWAERGLNA
HCCCCCCEEECHHHHEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
HRRPNDQGLFGIVQGAGFEDLRRQSARDLTSMDFAGYSIGGLAVGESHKEMNAVLDFTTP
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHCCCC
MLPEDKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIARNGTLMTHFGRVNIRNAKYEH
CCCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECEEEECCCCCCC
DFTPLDPMCDCYTCTNYTRAYLRHLIKADETFGLRLCSYHNLHFLVNLMKDVRGAIMDDN
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCH
LLEFREDFCERYGYNQPNAKDF
HHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TEHAIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQATVKTMSPEELKTLGSGI
CCHHHHHHHHHHHHCCCHHHCCEECCCCCCCCCEEEECCCCCEEECCCHHHHHHHCCCE
ILSNTYHLWLRPGDELVAEAGGLHKFMNWDQPILTDSGGFQVYSLVQNKKNITEEGVKFK
EEECEEEEEECCCHHHHHHHCCHHHHHCCCCCEEECCCCCEEHHHHHHHHCHHHHHHHHH
SHLDGRELFLNPEKAISIQNNLGSDIMMSFDECPPFYQPYDYVKASVERTSRWAERGLNA
HCCCCCCEEECHHHHEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
HRRPNDQGLFGIVQGAGFEDLRRQSARDLTSMDFAGYSIGGLAVGESHKEMNAVLDFTTP
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHCCCC
MLPEDKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIARNGTLMTHFGRVNIRNAKYEH
CCCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECEEEECCCCCCC
DFTPLDPMCDCYTCTNYTRAYLRHLIKADETFGLRLCSYHNLHFLVNLMKDVRGAIMDDN
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCH
LLEFREDFCERYGYNQPNAKDF
HHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471