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The map label for this gene is cafA

Identifier: 15834813

GI number: 15834813

Start: 225813

End: 227351

Strand: Reverse

Name: cafA

Synonym: TC0193

Alternate gene names: 15834813

Gene position: 227351-225813 (Counterclockwise)

Preceding gene: 15834818

Following gene: 15834812

Centisome position: 21.19

GC content: 38.14

Gene sequence:

>1539_bases
ATGGAAAACGATATCTTACTAAACATAGAGTCTAAAGAGATTCGTTACGCGCATTTAAAAAATGGCCAATTGTTTGATCT
AATCATTGAAAGAAAAAAGATCCGCCAGTTGAAAGGGAATATATATAGAGGTCGCGTAACTAATATTTTACGAAACATTC
AATCCGCCTTCATCAACATAGATGAGCGAGAGAATGGCTTTATCCACATTTCGGATGTATTAGAAAATTCTAAAAAATTC
GAACAGATGTTCGACATAGATGCGGATTCCGATCAATCCGAACCGCTGTCAGAAGAAACATCAGAAGCCCCTATTGAAGA
ACTTCTTAAGCTGGATAGCCCTGTACTCGTGCAGGTGGTTAAAGAACCTATAGGCACGAAAGGAGCTCGATTAACTTCTA
ATATTTCAATCCCAGGACGCTACTTGGTTCTTCTACCAAATTCCCCTCACCGAGGAGTTTCTCGTAAAATTGAAGATCCT
TTAATGCGAGATCAACTTAAACAGTTAATTCGATCATTTGAAATGCCTCAAAATATGGGGCTGATCTGCCGAACCGCCAG
CATTTCCGCTTCAACAGAAGCACTTATCAATGAAGCTCAAGATCTGCTGAATACGTGGCAAAGTATTTTAGAAAAATTTT
ATTCTCCAGATCATCCGTCGCTTCTTTATGAAGAAACAGACATTCTAAAAAAAGCGGTAATGACTTGCGTAGATAAAAGT
TATAAACGCTTGTTAATAGATGACTATGCCACTTATCAAAAGTGCAAACGTTTATTGAGTAAATATTCCCCAGATACAGC
TGTTAAGATCGAATATTATCGTGATTCCGTTCCCATGTTTGAACGTTTCAACATTGAGAAGGAAATTGATCGCGCTACGA
AACGGAAAATATGGCTTTCTAGTGGGGGATACCTCTTTTTCGATAAAACAGAGGCTATGCACACAATTGATGTGAATTCA
GGACGAAGTACACAACTCGAAAGTGGAGTTGAAGAGACTCTTGTCCAAATCAATCTAGAAGCCGCAGAAGAGATTGCTAG
ACAGCTCCGATTGCGAAACATTGGCGGACTTGTCATTATAGACTTTATCGACATGAAATCGCGTAAAAATCAGCGACGTG
TGTTAGAAAGACTAAAAGAACATATGAAATATGACGCGGCACGCTGTACAATTTTAAGCATGAGCGAATTTGGTTTAGTA
GAAATGACTCGACAAAGAAATCGTGAGTCATTGATGCAAACCCTATTTACAACTTGCCCCTATTGCAATGGGAATGCCAT
TATCAAAACCTCTGAGAGTATCCTTATAGAAATCGAACGTGATTTAAAGAAAATCATTAAGCATAAGGAACATACTCATT
TGTGTTTAGTCGTTCACCCGGAAATTGCTCACTATATGAAACAAGAGCAAGATGATGTCGAATTAGTCCGCTTAGCTAAA
CAATTGAAAGCTAAGCTACAGATCAATACGTCTGATTCTATCCATCTTAACCACTATCAATTTTTCTCATTAATCACAGG
AGAAGGAATTGAGCTATAA

Upstream 100 bases:

>100_bases
AACTTATAATCCGCCTTCTACCTAATAATTTTTAAAACAACTCCCTATCCTCGGGATCAGGTGAGAACTCCTGTTAGAGC
GCAGAGCATTTAGGGACGCC

Downstream 100 bases:

>100_bases
ATAGGTGTAGTGATGCATTTTTCAAACTATTTATATCAGGCTTTTGAAGATCTGCCAGAGCCTTTGTACCAAAAGTTTCA
AATTTGCCATCAAACTTACA

Product: ribonuclease G

Products: NA

Alternate protein names: RNase G; Cytoplasmic axial filament protein [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MENDILLNIESKEIRYAHLKNGQLFDLIIERKKIRQLKGNIYRGRVTNILRNIQSAFINIDERENGFIHISDVLENSKKF
EQMFDIDADSDQSEPLSEETSEAPIEELLKLDSPVLVQVVKEPIGTKGARLTSNISIPGRYLVLLPNSPHRGVSRKIEDP
LMRDQLKQLIRSFEMPQNMGLICRTASISASTEALINEAQDLLNTWQSILEKFYSPDHPSLLYEETDILKKAVMTCVDKS
YKRLLIDDYATYQKCKRLLSKYSPDTAVKIEYYRDSVPMFERFNIEKEIDRATKRKIWLSSGGYLFFDKTEAMHTIDVNS
GRSTQLESGVEETLVQINLEAAEEIARQLRLRNIGGLVIIDFIDMKSRKNQRRVLERLKEHMKYDAARCTILSMSEFGLV
EMTRQRNRESLMQTLFTTCPYCNGNAIIKTSESILIEIERDLKKIIKHKEHTHLCLVVHPEIAHYMKQEQDDVELVRLAK
QLKAKLQINTSDSIHLNHYQFFSLITGEGIEL

Sequences:

>Translated_512_residues
MENDILLNIESKEIRYAHLKNGQLFDLIIERKKIRQLKGNIYRGRVTNILRNIQSAFINIDERENGFIHISDVLENSKKF
EQMFDIDADSDQSEPLSEETSEAPIEELLKLDSPVLVQVVKEPIGTKGARLTSNISIPGRYLVLLPNSPHRGVSRKIEDP
LMRDQLKQLIRSFEMPQNMGLICRTASISASTEALINEAQDLLNTWQSILEKFYSPDHPSLLYEETDILKKAVMTCVDKS
YKRLLIDDYATYQKCKRLLSKYSPDTAVKIEYYRDSVPMFERFNIEKEIDRATKRKIWLSSGGYLFFDKTEAMHTIDVNS
GRSTQLESGVEETLVQINLEAAEEIARQLRLRNIGGLVIIDFIDMKSRKNQRRVLERLKEHMKYDAARCTILSMSEFGLV
EMTRQRNRESLMQTLFTTCPYCNGNAIIKTSESILIEIERDLKKIIKHKEHTHLCLVVHPEIAHYMKQEQDDVELVRLAK
QLKAKLQINTSDSIHLNHYQFFSLITGEGIEL
>Mature_512_residues
MENDILLNIESKEIRYAHLKNGQLFDLIIERKKIRQLKGNIYRGRVTNILRNIQSAFINIDERENGFIHISDVLENSKKF
EQMFDIDADSDQSEPLSEETSEAPIEELLKLDSPVLVQVVKEPIGTKGARLTSNISIPGRYLVLLPNSPHRGVSRKIEDP
LMRDQLKQLIRSFEMPQNMGLICRTASISASTEALINEAQDLLNTWQSILEKFYSPDHPSLLYEETDILKKAVMTCVDKS
YKRLLIDDYATYQKCKRLLSKYSPDTAVKIEYYRDSVPMFERFNIEKEIDRATKRKIWLSSGGYLFFDKTEAMHTIDVNS
GRSTQLESGVEETLVQINLEAAEEIARQLRLRNIGGLVIIDFIDMKSRKNQRRVLERLKEHMKYDAARCTILSMSEFGLV
EMTRQRNRESLMQTLFTTCPYCNGNAIIKTSESILIEIERDLKKIIKHKEHTHLCLVVHPEIAHYMKQEQDDVELVRLAK
QLKAKLQINTSDSIHLNHYQFFSLITGEGIEL

Specific function: Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm, cytoskeleton [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=491, Percent_Identity=35.4378818737271, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI1787325, Length=507, Percent_Identity=33.9250493096647, Blast_Score=285, Evalue=5e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: 3.1.4.- [C]

Molecular weight: Translated: 59272; Mature: 59272

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENDILLNIESKEIRYAHLKNGQLFDLIIERKKIRQLKGNIYRGRVTNILRNIQSAFINI
CCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEC
DERENGFIHISDVLENSKKFEQMFDIDADSDQSEPLSEETSEAPIEELLKLDSPVLVQVV
CCCCCCEEEEHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHCCCHHHHHHHCCCHHHHHHH
KEPIGTKGARLTSNISIPGRYLVLLPNSPHRGVSRKIEDPLMRDQLKQLIRSFEMPQNMG
HHCCCCCCCEEEECCCCCCEEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCC
LICRTASISASTEALINEAQDLLNTWQSILEKFYSPDHPSLLYEETDILKKAVMTCVDKS
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
YKRLLIDDYATYQKCKRLLSKYSPDTAVKIEYYRDSVPMFERFNIEKEIDRATKRKIWLS
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHCCCHHHHHHHHHCEEEEE
SGGYLFFDKTEAMHTIDVNSGRSTQLESGVEETLVQINLEAAEEIARQLRLRNIGGLVII
CCCEEEEECCCCEEEEECCCCCCCHHHCCHHHHHHHEEHHHHHHHHHHHHHHCCCCEEEE
DFIDMKSRKNQRRVLERLKEHMKYDAARCTILSMSEFGLVEMTRQRNRESLMQTLFTTCP
EEECCCCCCHHHHHHHHHHHHHHHCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
YCNGNAIIKTSESILIEIERDLKKIIKHKEHTHLCLVVHPEIAHYMKQEQDDVELVRLAK
CCCCCEEEEECCCEEEEEHHHHHHHHHCCCCCEEEEEECHHHHHHHHCCCCHHHHHHHHH
QLKAKLQINTSDSIHLNHYQFFSLITGEGIEL
HHHHEEEECCCCCEEECCEEEEEEECCCCCCC
>Mature Secondary Structure
MENDILLNIESKEIRYAHLKNGQLFDLIIERKKIRQLKGNIYRGRVTNILRNIQSAFINI
CCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEC
DERENGFIHISDVLENSKKFEQMFDIDADSDQSEPLSEETSEAPIEELLKLDSPVLVQVV
CCCCCCEEEEHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHCCCHHHHHHHCCCHHHHHHH
KEPIGTKGARLTSNISIPGRYLVLLPNSPHRGVSRKIEDPLMRDQLKQLIRSFEMPQNMG
HHCCCCCCCEEEECCCCCCEEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCC
LICRTASISASTEALINEAQDLLNTWQSILEKFYSPDHPSLLYEETDILKKAVMTCVDKS
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
YKRLLIDDYATYQKCKRLLSKYSPDTAVKIEYYRDSVPMFERFNIEKEIDRATKRKIWLS
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHCCCHHHHHHHHHCEEEEE
SGGYLFFDKTEAMHTIDVNSGRSTQLESGVEETLVQINLEAAEEIARQLRLRNIGGLVII
CCCEEEEECCCCEEEEECCCCCCCHHHCCHHHHHHHEEHHHHHHHHHHHHHHCCCCEEEE
DFIDMKSRKNQRRVLERLKEHMKYDAARCTILSMSEFGLVEMTRQRNRESLMQTLFTTCP
EEECCCCCCHHHHHHHHHHHHHHHCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
YCNGNAIIKTSESILIEIERDLKKIIKHKEHTHLCLVVHPEIAHYMKQEQDDVELVRLAK
CCCCCEEEEECCCEEEEEHHHHHHHHHCCCCCEEEEEECHHHHHHHHCCCCHHHHHHHHH
QLKAKLQINTSDSIHLNHYQFFSLITGEGIEL
HHHHEEEECCCCCEEECCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]