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The map label for this gene is priA

Identifier: 15834779

GI number: 15834779

Start: 191964

End: 194225

Strand: Reverse

Name: priA

Synonym: TC0159

Alternate gene names: 15834779

Gene position: 194225-191964 (Counterclockwise)

Preceding gene: 15834780

Following gene: 15834775

Centisome position: 18.1

GC content: 40.58

Gene sequence:

>2262_bases
ATGGACCCTACTCACCAATCTTTCCGCCTCTACGCAGAAGTCTTAGTCAGTGCAAATATCAATAAAATCCTGGACTACGG
CATTCCTGCTGAATTGGAAGACCGAGTAACTATAGGATCTGTTGTTAAAGTTCCCTTACAAAGAAAAGTTACAAATGACA
AATATAAGTTTGCTATTGTTCTCAAAATTAAAGATTCTTCAGATTTTGCTAATGTAATTCAGCCTATTGCAGGGATTAGT
TACGAAGGGGTTACCCTCCCAAAAGATCTCATTGATTTGATTTTCTGGATCAGTCAGTACTACTTTTGCCCTTTAGGAAG
TACATTATCCCTATTCCTCCCCACCGTATACTCACAGACACATTCAACCAAACACCAGAATAACGTATTTCTAGGACAAA
ATGCTGAGCGTACGCAAGAAATCATAAAATCCATTGAAGATCCCCATCAAATTGCAGTCCTTCGCAAATTACTCAAAACT
ACAAAGCCCCTTACTCCCAAAGAACTGATCAAAAAAACCGACATATCTACAAAAATTTTAGATAGCTTATCTAAACAAGG
ATTCATCCGTATAGTAGACTCTGCCAATTTGGAGATCCAAGACGAACAATTACAATACTTCCTTCCAGAGCCTCCAACAC
TGACTCAAGAGCAAATAGAAGCTATCAACACGATCTCCCAATCATTAATTACCACCAAGTTTCAAACATGCTTATTATTT
GGAGTAACTGGAAGTGGAAAAACAGAAGTGTATCTTCAAGTTATTCGTAAAGCTAGGTCCCTGGGGAAAAGTGTCATTCT
CCTAGTTCCTGAGGTTGCTTTGACTATTCAAACCCTCTCCTTTTTTAAAATGCACTTTGGTGCTGAAGTGGGCGTGTTGC
ACTACAAGTTAAGCGAAAGCGAGCGTACCCAAACCTGGCACAAAGCTGCTCGAGGGTTAATTAATATCATCATTGGCCCT
CGCTCTGCGATTTTTTGTCCTATGCAGAATCTGGGATTGATCATCGTTGATGAGGAGCATGACGGCGCCTATAAACAAAG
CGATTTACCACCTTTTTATCAAGCTCGAGATGTAGCAGTCATGCGAGGGAAATTCACTAATGCTACAGTCATTCTAGGCA
GCGCTACTCCAAGTTTAGAAAGCTATACCAATGCTTTATCTAAAAAATATACCCTTTCTGTCCTTTCTAAACGGGCTTCT
ACATCAATCCCCACTAAGGTTTCCCTCATTGATATGAATAGGGAAATGGAAAAAACGCGAAAAAAGATCCTCTTTTCTCC
AACAGTTATTCGAAGTATTGAACAACGCTTAGCAGTTGGAGAACAAACGATCATTTTCTTTAATCGCCGAGGATTTCACA
CCAATGTGTCCTGCTCATCATGCAAATACACCTTGAAGTGCCCTCACTGTGACATGATCCTTACATTTCATAAAACCGAG
CGTATTCTCCTATGCCACCTTTGTAATACGCGATTATCTAAACCTATTACATCATGTCCTCAATGCCATGGCACTATGAC
ATTGCAATACCGGGGGGCAGGAACAGAAAAAATCGAGGCCCTTCTCCAAGACTTTTTCCCAACAGTCCGCACTATACGCT
TAGACTCTGACACCACGCGCTATCGAGGAAGTCATGACGCATTAATTAAACAATTCGCTACCGGGAAGGCAGATATTCTT
ATCGGAACACAAATGATTGCAAAAGGAATGCACTTTCCTGCAGTCACTCTTTCTGTAGTTTTAAGTGGAGATTCCGGCCT
ATACATCCCAGATTTCCGAGCTGCTGAACAAGTTTTCCAACTGATTACACAGGTCACTGGACGATCAGGCCGTAGTTACC
TTCCTGGAGAAGTTCTGATTCAGACTTTTCTTCCACAAAATTCCACTATATCACATGCATTAGCTCAAGATTTTCCAGCC
TTTTACAAAGAAGAAATTTTAGGAAGAAAAGCATGTAACTATCCCCCTTTTACTAGACTCATCCGCTGTATTTTCCTTGG
GAAATGTGCAGAATATACCTTGCAAGAGGCCCAACGCATGCACGAGCTAATCAAACAAAATTTGGATGCTCAGGCTTCTC
TTATGGACATATCCCCCTGTGGGCACTTCAAAGTAAAAGACCTTTTTCACTATCAATTTTTGATCAAAACTCGCAACATT
CTCACTGTAAATAAACAGCTTCAGGATGCATTTTCAGCTGCTAAACTTTCTTCTAAAGTACGATGCATCGTGGACATAGA
TCCCATTACTACATTCTTTTGA

Upstream 100 bases:

>100_bases
AAATCCAATACCATACCCCGATTTTGCAAAATGAAATAACCTGCATACCCATTTATTCCTCTTCCCAGTACGAAAAAGAT
ACAGCATTAAAAAGAGATTT

Downstream 100 bases:

>100_bases
TCTGATACTTGCGAAAGTATCGAAACCAAAATATCCACTTCCTCTTTAGTATTAAAAGCATGCAAATTGGCTCGCAAAGA
TCCCTTTGCAGGGAATCGCG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y

Number of amino acids: Translated: 753; Mature: 753

Protein sequence:

>753_residues
MDPTHQSFRLYAEVLVSANINKILDYGIPAELEDRVTIGSVVKVPLQRKVTNDKYKFAIVLKIKDSSDFANVIQPIAGIS
YEGVTLPKDLIDLIFWISQYYFCPLGSTLSLFLPTVYSQTHSTKHQNNVFLGQNAERTQEIIKSIEDPHQIAVLRKLLKT
TKPLTPKELIKKTDISTKILDSLSKQGFIRIVDSANLEIQDEQLQYFLPEPPTLTQEQIEAINTISQSLITTKFQTCLLF
GVTGSGKTEVYLQVIRKARSLGKSVILLVPEVALTIQTLSFFKMHFGAEVGVLHYKLSESERTQTWHKAARGLINIIIGP
RSAIFCPMQNLGLIIVDEEHDGAYKQSDLPPFYQARDVAVMRGKFTNATVILGSATPSLESYTNALSKKYTLSVLSKRAS
TSIPTKVSLIDMNREMEKTRKKILFSPTVIRSIEQRLAVGEQTIIFFNRRGFHTNVSCSSCKYTLKCPHCDMILTFHKTE
RILLCHLCNTRLSKPITSCPQCHGTMTLQYRGAGTEKIEALLQDFFPTVRTIRLDSDTTRYRGSHDALIKQFATGKADIL
IGTQMIAKGMHFPAVTLSVVLSGDSGLYIPDFRAAEQVFQLITQVTGRSGRSYLPGEVLIQTFLPQNSTISHALAQDFPA
FYKEEILGRKACNYPPFTRLIRCIFLGKCAEYTLQEAQRMHELIKQNLDAQASLMDISPCGHFKVKDLFHYQFLIKTRNI
LTVNKQLQDAFSAAKLSSKVRCIVDIDPITTFF

Sequences:

>Translated_753_residues
MDPTHQSFRLYAEVLVSANINKILDYGIPAELEDRVTIGSVVKVPLQRKVTNDKYKFAIVLKIKDSSDFANVIQPIAGIS
YEGVTLPKDLIDLIFWISQYYFCPLGSTLSLFLPTVYSQTHSTKHQNNVFLGQNAERTQEIIKSIEDPHQIAVLRKLLKT
TKPLTPKELIKKTDISTKILDSLSKQGFIRIVDSANLEIQDEQLQYFLPEPPTLTQEQIEAINTISQSLITTKFQTCLLF
GVTGSGKTEVYLQVIRKARSLGKSVILLVPEVALTIQTLSFFKMHFGAEVGVLHYKLSESERTQTWHKAARGLINIIIGP
RSAIFCPMQNLGLIIVDEEHDGAYKQSDLPPFYQARDVAVMRGKFTNATVILGSATPSLESYTNALSKKYTLSVLSKRAS
TSIPTKVSLIDMNREMEKTRKKILFSPTVIRSIEQRLAVGEQTIIFFNRRGFHTNVSCSSCKYTLKCPHCDMILTFHKTE
RILLCHLCNTRLSKPITSCPQCHGTMTLQYRGAGTEKIEALLQDFFPTVRTIRLDSDTTRYRGSHDALIKQFATGKADIL
IGTQMIAKGMHFPAVTLSVVLSGDSGLYIPDFRAAEQVFQLITQVTGRSGRSYLPGEVLIQTFLPQNSTISHALAQDFPA
FYKEEILGRKACNYPPFTRLIRCIFLGKCAEYTLQEAQRMHELIKQNLDAQASLMDISPCGHFKVKDLFHYQFLIKTRNI
LTVNKQLQDAFSAAKLSSKVRCIVDIDPITTFF
>Mature_753_residues
MDPTHQSFRLYAEVLVSANINKILDYGIPAELEDRVTIGSVVKVPLQRKVTNDKYKFAIVLKIKDSSDFANVIQPIAGIS
YEGVTLPKDLIDLIFWISQYYFCPLGSTLSLFLPTVYSQTHSTKHQNNVFLGQNAERTQEIIKSIEDPHQIAVLRKLLKT
TKPLTPKELIKKTDISTKILDSLSKQGFIRIVDSANLEIQDEQLQYFLPEPPTLTQEQIEAINTISQSLITTKFQTCLLF
GVTGSGKTEVYLQVIRKARSLGKSVILLVPEVALTIQTLSFFKMHFGAEVGVLHYKLSESERTQTWHKAARGLINIIIGP
RSAIFCPMQNLGLIIVDEEHDGAYKQSDLPPFYQARDVAVMRGKFTNATVILGSATPSLESYTNALSKKYTLSVLSKRAS
TSIPTKVSLIDMNREMEKTRKKILFSPTVIRSIEQRLAVGEQTIIFFNRRGFHTNVSCSSCKYTLKCPHCDMILTFHKTE
RILLCHLCNTRLSKPITSCPQCHGTMTLQYRGAGTEKIEALLQDFFPTVRTIRLDSDTTRYRGSHDALIKQFATGKADIL
IGTQMIAKGMHFPAVTLSVVLSGDSGLYIPDFRAAEQVFQLITQVTGRSGRSYLPGEVLIQTFLPQNSTISHALAQDFPA
FYKEEILGRKACNYPPFTRLIRCIFLGKCAEYTLQEAQRMHELIKQNLDAQASLMDISPCGHFKVKDLFHYQFLIKTRNI
LTVNKQLQDAFSAAKLSSKVRCIVDIDPITTFF

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain

Homologues:

Organism=Escherichia coli, GI1790370, Length=753, Percent_Identity=32.2709163346614, Blast_Score=377, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIA_CHLMU (Q9PLE5)

Other databases:

- EMBL:   AE002160
- PIR:   D81734
- RefSeq:   NP_296538.1
- ProteinModelPortal:   Q9PLE5
- SMR:   Q9PLE5
- GeneID:   1245693
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0159
- TIGR:   TC_0159
- HOGENOM:   HBG725563
- OMA:   RTIRIDS
- PhylomeDB:   Q9PLE5
- ProtClustDB:   PRK05580
- BioCyc:   CMUR243161:TC_0159-MONOMER
- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259
- InterPro:   IPR006935
- SMART:   SM00487
- TIGRFAMs:   TIGR00595

Pfam domain/function: PF00271 Helicase_C; PF04851 ResIII

EC number: NA

Molecular weight: Translated: 84766; Mature: 84766

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS51192 HELICASE_ATP_BIND_1; PS51194 HELICASE_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPTHQSFRLYAEVLVSANINKILDYGIPAELEDRVTIGSVVKVPLQRKVTNDKYKFAIV
CCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEECHHHHCCHHHCCCCCCEEEEEE
LKIKDSSDFANVIQPIAGISYEGVTLPKDLIDLIFWISQYYFCPLGSTLSLFLPTVYSQT
EEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
HSTKHQNNVFLGQNAERTQEIIKSIEDPHQIAVLRKLLKTTKPLTPKELIKKTDISTKIL
CCCCCCCCEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHH
DSLSKQGFIRIVDSANLEIQDEQLQYFLPEPPTLTQEQIEAINTISQSLITTKFQTCLLF
HHHCCCCEEEEEECCCCEEECCHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEE
GVTGSGKTEVYLQVIRKARSLGKSVILLVPEVALTIQTLSFFKMHFGAEVGVLHYKLSES
EECCCCCHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEEEECCC
ERTQTWHKAARGLINIIIGPRSAIFCPMQNLGLIIVDEEHDGAYKQSDLPPFYQARDVAV
HHHHHHHHHHCCEEEEEECCCCEEECCCCCCCEEEEECCCCCCCCCCCCCCCHHHCCHHE
MRGKFTNATVILGSATPSLESYTNALSKKYTLSVLSKRASTSIPTKVSLIDMNREMEKTR
EECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHH
KKILFSPTVIRSIEQRLAVGEQTIIFFNRRGFHTNVSCSSCKYTLKCPHCDMILTFHKTE
HHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEEECCC
RILLCHLCNTRLSKPITSCPQCHGTMTLQYRGAGTEKIEALLQDFFPTVRTIRLDSDTTR
EEEEEEHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCC
YRGSHDALIKQFATGKADILIGTQMIAKGMHFPAVTLSVVLSGDSGLYIPDFRAAEQVFQ
CCCCHHHHHHHHCCCCCEEEEEHHHHHCCCCCCEEEEEEEEECCCCEECCCHHHHHHHHH
LITQVTGRSGRSYLPGEVLIQTFLPQNSTISHALAQDFPAFYKEEILGRKACNYPPFTRL
HHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
IRCIFLGKCAEYTLQEAQRMHELIKQNLDAQASLMDISPCGHFKVKDLFHYQFLIKTRNI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHEEEECCE
LTVNKQLQDAFSAAKLSSKVRCIVDIDPITTFF
EEECHHHHHHHHHHHHCCCCEEEEEECCHHCCC
>Mature Secondary Structure
MDPTHQSFRLYAEVLVSANINKILDYGIPAELEDRVTIGSVVKVPLQRKVTNDKYKFAIV
CCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEECHHHHCCHHHCCCCCCEEEEEE
LKIKDSSDFANVIQPIAGISYEGVTLPKDLIDLIFWISQYYFCPLGSTLSLFLPTVYSQT
EEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
HSTKHQNNVFLGQNAERTQEIIKSIEDPHQIAVLRKLLKTTKPLTPKELIKKTDISTKIL
CCCCCCCCEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHH
DSLSKQGFIRIVDSANLEIQDEQLQYFLPEPPTLTQEQIEAINTISQSLITTKFQTCLLF
HHHCCCCEEEEEECCCCEEECCHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEE
GVTGSGKTEVYLQVIRKARSLGKSVILLVPEVALTIQTLSFFKMHFGAEVGVLHYKLSES
EECCCCCHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEEEECCC
ERTQTWHKAARGLINIIIGPRSAIFCPMQNLGLIIVDEEHDGAYKQSDLPPFYQARDVAV
HHHHHHHHHHCCEEEEEECCCCEEECCCCCCCEEEEECCCCCCCCCCCCCCCHHHCCHHE
MRGKFTNATVILGSATPSLESYTNALSKKYTLSVLSKRASTSIPTKVSLIDMNREMEKTR
EECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHH
KKILFSPTVIRSIEQRLAVGEQTIIFFNRRGFHTNVSCSSCKYTLKCPHCDMILTFHKTE
HHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEEECCC
RILLCHLCNTRLSKPITSCPQCHGTMTLQYRGAGTEKIEALLQDFFPTVRTIRLDSDTTR
EEEEEEHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCC
YRGSHDALIKQFATGKADILIGTQMIAKGMHFPAVTLSVVLSGDSGLYIPDFRAAEQVFQ
CCCCHHHHHHHHCCCCCEEEEEHHHHHCCCCCCEEEEEEEEECCCCEECCCHHHHHHHHH
LITQVTGRSGRSYLPGEVLIQTFLPQNSTISHALAQDFPAFYKEEILGRKACNYPPFTRL
HHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
IRCIFLGKCAEYTLQEAQRMHELIKQNLDAQASLMDISPCGHFKVKDLFHYQFLIKTRNI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHEEEECCE
LTVNKQLQDAFSAAKLSSKVRCIVDIDPITTFF
EEECHHHHHHHHHHHHCCCCEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935