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The map label for this gene is nifS

Identifier: 13358017

GI number: 13358017

Start: 514561

End: 515769

Strand: Reverse

Name: nifS

Synonym: UU454

Alternate gene names: 13358017

Gene position: 515769-514561 (Counterclockwise)

Preceding gene: 13358020

Following gene: 13358016

Centisome position: 68.61

GC content: 26.96

Gene sequence:

>1209_bases
ATGAATGATTACAAACAATTTTTTCCTTGATTTAAAAATAACAAAGATGTTATTTATTTAGATTCAAGTGCAACTTCATT
AAAACCTCAAGTTGTAATCGATGCAATTGTTGATTATTATACAAAATATTCTACTAACCCCCATAATACTGATTCAAATT
TTACTTTTCACACTCATGAAATTATGAATGAAACAAGAGCAAATGTTGCCAAATTCATCAATGCTAATTTTGATGAAATC
ATTTTTACAAGTGGTGCTACAGAATCTCTAAATTTGATTGCAAACGGTTTAAGACCTCATTTAAAAAAGGGTGATGAAAT
TATTTTAACTTATATTGAACATGCTTCTAATTTATTACCATGATACAAACTTCGAGATGATTTAGGTGTCAAGATTATTT
TTGCTAATCAAAAAAATCAATTTCCACAACTTAGTGATTTTCTAAAAGTAATTTCACCTAAAACAAAAGTGGTTTCGTTT
GCTTCTGGTGGTAATTTAATTGGTAATGTTTTAGATGAAAATCTTATTATTAAAAATATTAAAAAAATTAAACCAGATGT
ATTAGTGTGCGTTGATGCAACTCAAAGTATTCAACACCGTATGTTTGATGTGAAAAAATGCAAAAGTGATTTTATGGTCT
TTTCAGCACATAAATTATTAGGACCTACAGGAATTGGTGTTGCTTATATTAAAAATGACTTGATTAAAAAAATGCAACCA
TTAAAATATGGTGGTGGAATGAATTTTAGCATTGATTTAAATAGTTATCAGCTCTATGATAATTACATGAAATTTGAGGG
TGGAACTCCGCATGTTGCTGGTTTTTATGGTTTTAATGCTGCTTTAAAATTTTTAATGGATATAGGTTATAATAAAATTC
ATGAACATGAATTAAAAATTACTAAATATGCACGTGACCAATTAGCTTTAATTCCTGAAATTAAAACATATGTGCAAGAT
GCTACTTCAAGTACTATAACTTTTAGTTATAATGGTGTTTTTTGCCAAGATTTTGCTAATTATTTAGGATCAAAAAATAT
TATTGTGCGAAGTGGATTATCATGTGCAAAAATTATTAATAACGTGATTCAAACCGAATGTGCTATTCGTGCATCTTTTT
ACATATATAATGATTTTAATGATGTTGATAGATTAATTAAGGCAATTAAAGAATATCAAAAAGGAGATGAATTAAATGGC
ATCTTATAA

Upstream 100 bases:

>100_bases
GCTGACAAAAGCAAACTTTGTTATTGTTTTTAACAAATTTAATTATCAAGCCATCGCAAGAAATTTAGTCTTATTTTTGA
AAAATAAGCAAGGATACGCT

Downstream 100 bases:

>100_bases
TATCAATGACAATCTTTTATTACGTAAGATTATCATGCAACACTATGAACACCCTAAAAATAAAGTTTGTTTTGTACAAG
ATGAAATAAATTATTTGTCA

Product: nitrogen fixation protein - class-V pyridoxal-phosphate aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MNDYKQFFPWFKNNKDVIYLDSSATSLKPQVVIDAIVDYYTKYSTNPHNTDSNFTFHTHEIMNETRANVAKFINANFDEI
IFTSGATESLNLIANGLRPHLKKGDEIILTYIEHASNLLPWYKLRDDLGVKIIFANQKNQFPQLSDFLKVISPKTKVVSF
ASGGNLIGNVLDENLIIKNIKKIKPDVLVCVDATQSIQHRMFDVKKCKSDFMVFSAHKLLGPTGIGVAYIKNDLIKKMQP
LKYGGGMNFSIDLNSYQLYDNYMKFEGGTPHVAGFYGFNAALKFLMDIGYNKIHEHELKITKYARDQLALIPEIKTYVQD
ATSSTITFSYNGVFCQDFANYLGSKNIIVRSGLSCAKIINNVIQTECAIRASFYIYNDFNDVDRLIKAIKEYQKGDELNG
IL

Sequences:

>Translated_402_residues
MNDYKQFFP*FKNNKDVIYLDSSATSLKPQVVIDAIVDYYTKYSTNPHNTDSNFTFHTHEIMNETRANVAKFINANFDEI
IFTSGATESLNLIANGLRPHLKKGDEIILTYIEHASNLLP*YKLRDDLGVKIIFANQKNQFPQLSDFLKVISPKTKVVSF
ASGGNLIGNVLDENLIIKNIKKIKPDVLVCVDATQSIQHRMFDVKKCKSDFMVFSAHKLLGPTGIGVAYIKNDLIKKMQP
LKYGGGMNFSIDLNSYQLYDNYMKFEGGTPHVAGFYGFNAALKFLMDIGYNKIHEHELKITKYARDQLALIPEIKTYVQD
ATSSTITFSYNGVFCQDFANYLGSKNIIVRSGLSCAKIINNVIQTECAIRASFYIYNDFNDVDRLIKAIKEYQKGDELNG
IL
>Mature_402_residues
MNDYKQFFP*FKNNKDVIYLDSSATSLKPQVVIDAIVDYYTKYSTNPHNTDSNFTFHTHEIMNETRANVAKFINANFDEI
IFTSGATESLNLIANGLRPHLKKGDEIILTYIEHASNLLP*YKLRDDLGVKIIFANQKNQFPQLSDFLKVISPKTKVVSF
ASGGNLIGNVLDENLIIKNIKKIKPDVLVCVDATQSIQHRMFDVKKCKSDFMVFSAHKLLGPTGIGVAYIKNDLIKKMQP
LKYGGGMNFSIDLNSYQLYDNYMKFEGGTPHVAGFYGFNAALKFLMDIGYNKIHEHELKITKYARDQLALIPEIKTYVQD
ATSSTITFSYNGVFCQDFANYLGSKNIIVRSGLSCAKIINNVIQTECAIRASFYIYNDFNDVDRLIKAIKEYQKGDELNG
IL

Specific function: Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L- selenocystine to produce L-alanine [H]

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=232, Percent_Identity=31.8965517241379, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI156713448, Length=294, Percent_Identity=26.1904761904762, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1789175, Length=389, Percent_Identity=32.6478149100257, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1787970, Length=389, Percent_Identity=31.1053984575836, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI48994898, Length=232, Percent_Identity=32.3275862068966, Blast_Score=103, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI25143064, Length=236, Percent_Identity=30.5084745762712, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319831, Length=235, Percent_Identity=34.468085106383, Blast_Score=116, Evalue=6e-27,
Organism=Drosophila melanogaster, GI20129463, Length=230, Percent_Identity=31.304347826087, Blast_Score=109, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 45228; Mature: 45228

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDYKQFFPFKNNKDVIYLDSSATSLKPQVVIDAIVDYYTKYSTNPHNTDSNFTFHTHEI
CCCCHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHH
MNETRANVAKFINANFDEIIFTSGATESLNLIANGLRPHLKKGDEIILTYIEHASNLLPY
HHHHHHHHHHHHCCCCHHEEEECCCCHHHHHHHHCCCHHHCCCCCEEEEEEHHHCCCCCE
KLRDDLGVKIIFANQKNQFPQLSDFLKVISPKTKVVSFASGGNLIGNVLDENLIIKNIKK
EECCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHCCHHHHHHHHH
IKPDVLVCVDATQSIQHRMFDVKKCKSDFMVFSAHKLLGPTGIGVAYIKNDLIKKMQPLK
CCCCEEEEECCHHHHHHHHHHHHHHHHHHEEEEHHHHCCCCCCHHHHHHHHHHHHHCCCC
YGGGMNFSIDLNSYQLYDNYMKFEGGTPHVAGFYGFNAALKFLMDIGYNKIHEHELKITK
CCCCCEEEEECCCEEEEHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHH
YARDQLALIPEIKTYVQDATSSTITFSYNGVFCQDFANYLGSKNIIVRSGLSCAKIINNV
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEHHHHHHHCCCCCEEEECCCHHHHHHHHH
IQTECAIRASFYIYNDFNDVDRLIKAIKEYQKGDELNGIL
HHHHHEEEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNDYKQFFPFKNNKDVIYLDSSATSLKPQVVIDAIVDYYTKYSTNPHNTDSNFTFHTHEI
CCCCHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHH
MNETRANVAKFINANFDEIIFTSGATESLNLIANGLRPHLKKGDEIILTYIEHASNLLPY
HHHHHHHHHHHHCCCCHHEEEECCCCHHHHHHHHCCCHHHCCCCCEEEEEEHHHCCCCCE
KLRDDLGVKIIFANQKNQFPQLSDFLKVISPKTKVVSFASGGNLIGNVLDENLIIKNIKK
EECCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHCCHHHHHHHHH
IKPDVLVCVDATQSIQHRMFDVKKCKSDFMVFSAHKLLGPTGIGVAYIKNDLIKKMQPLK
CCCCEEEEECCHHHHHHHHHHHHHHHHHHEEEEHHHHCCCCCCHHHHHHHHHHHHHCCCC
YGGGMNFSIDLNSYQLYDNYMKFEGGTPHVAGFYGFNAALKFLMDIGYNKIHEHELKITK
CCCCCEEEEECCCEEEEHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHH
YARDQLALIPEIKTYVQDATSSTITFSYNGVFCQDFANYLGSKNIIVRSGLSCAKIINNV
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEHHHHHHHCCCCCEEEECCCHHHHHHHHH
IQTECAIRASFYIYNDFNDVDRLIKAIKEYQKGDELNGIL
HHHHHEEEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11048724 [H]