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The map label for this gene is metK

Identifier: 13357974

GI number: 13357974

Start: 474916

End: 476046

Strand: Direct

Name: metK

Synonym: UU412

Alternate gene names: 13357974

Gene position: 474916-476046 (Clockwise)

Preceding gene: 13357973

Following gene: 13357982

Centisome position: 63.18

GC content: 29.89

Gene sequence:

>1131_bases
ATGAAATATAAAAAAATTATAACGAGCGAATCTGTTGGTGCAGGCCATCCAGATAAAATATGTGATCAGATTAGTGATGC
AATTTTAGATGAATGCTTAGCACAAGATAAAAATTCACGTGTAGCTTGTGAAGTTTTAGCTTGTAATCGTTTAATTGTTA
TTGCTGGGGAAATTACCACGCATGCATATGTTGATGTAGTAAAAACCGCTTGAGAAATTATTAAACCTTTAGGCTATAAT
GAAAATGATTTTACGATTATTTCTAATGTTAATAAACAATCAGTAGATATAGCGCAAAGTGTTAATAAAGCAAATAAAGA
TTTAATTGGAGCTGGTGACCAAGGAATTGTTTTTGGCTATGCTTGTGATGAAACACCTCAATATATGCCATTAACAACAG
TGTTAGCTCATGAATTACTAAAAGAAATCGAAAAACAAAGACGTAATAAAGAATTTGTTAAAATTCAAGCTGATATGAAA
TCACAAGTAAGTATTGATTATTCTAATTGTACGCCTGTTATTGAGACAATGTTGGTAAGTATTCAACATGATGAAGATTA
TAACATTGAATATTTTAATAAAAAAGTTAGCACAATTATGGATCAAATTGCTATAAAATATGGTTTAAATACCGATTTTA
AAAAAATTATTAATTCCTCAGGGAGATTTGTAATTGGTGGTCCAATTGGTGACACTGGTTTAACTGGAAGAAAAATTATC
GTAGATACTTATGGAGGAATAGGTCATCATGGTGGTGGTGCTTTTAGTGGCAAAGACCCTACTAAAGTTGATCGTAGTGC
TTCATACTTTGCACGTTGAATAGCTAAAAATGTTGTTGCAGCTAAGCTTGCTAAACAGTGTGAAATTCAATTAGCTTTTG
CAATTGGAGAACCACAACCTGTGGCAATGTTTGTTAATACATTCAAGACTAATTTGGTTGATGAAAAGAAAATTTTTGAA
GCAATTAATAAAAGTTTTAATTTTGATATCAAAACGTTTATTAATGATTTAGAATTGTGAACAACTAAATATTTACCAGT
AGCAACTTATGGTCATTTCGGTCGTGATGATTTAAACTTAAGTTGAGAAAAATTAAATAAGGTTCAAGATTTAATAAAAT
ATAGTAAATAA

Upstream 100 bases:

>100_bases
GTGATAATGCTGCTATGATTGGGGCTTATGCTTATGCTTTAATTAAAAATCATAAGAAATCAATATTAATAAAATAATGG
TAGAAAGAAAAGAATTTAGT

Downstream 100 bases:

>100_bases
ATTTAAAAAACGATACGCACAATAAAGGGCATATCGTTTTTTTAATAAATAATATAATGGTGCTCAAGAAGGGACTCGAA
CCCTTATGATTTCTCGGTTG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKYKKIITSESVGAGHPDKICDQISDAILDECLAQDKNSRVACEVLACNRLIVIAGEITTHAYVDVVKTAWEIIKPLGYN
ENDFTIISNVNKQSVDIAQSVNKANKDLIGAGDQGIVFGYACDETPQYMPLTTVLAHELLKEIEKQRRNKEFVKIQADMK
SQVSIDYSNCTPVIETMLVSIQHDEDYNIEYFNKKVSTIMDQIAIKYGLNTDFKKIINSSGRFVIGGPIGDTGLTGRKII
VDTYGGIGHHGGGAFSGKDPTKVDRSASYFARWIAKNVVAAKLAKQCEIQLAFAIGEPQPVAMFVNTFKTNLVDEKKIFE
AINKSFNFDIKTFINDLELWTTKYLPVATYGHFGRDDLNLSWEKLNKVQDLIKYSK

Sequences:

>Translated_376_residues
MKYKKIITSESVGAGHPDKICDQISDAILDECLAQDKNSRVACEVLACNRLIVIAGEITTHAYVDVVKTA*EIIKPLGYN
ENDFTIISNVNKQSVDIAQSVNKANKDLIGAGDQGIVFGYACDETPQYMPLTTVLAHELLKEIEKQRRNKEFVKIQADMK
SQVSIDYSNCTPVIETMLVSIQHDEDYNIEYFNKKVSTIMDQIAIKYGLNTDFKKIINSSGRFVIGGPIGDTGLTGRKII
VDTYGGIGHHGGGAFSGKDPTKVDRSASYFAR*IAKNVVAAKLAKQCEIQLAFAIGEPQPVAMFVNTFKTNLVDEKKIFE
AINKSFNFDIKTFINDLEL*TTKYLPVATYGHFGRDDLNLS*EKLNKVQDLIKYSK
>Mature_376_residues
MKYKKIITSESVGAGHPDKICDQISDAILDECLAQDKNSRVACEVLACNRLIVIAGEITTHAYVDVVKTA*EIIKPLGYN
ENDFTIISNVNKQSVDIAQSVNKANKDLIGAGDQGIVFGYACDETPQYMPLTTVLAHELLKEIEKQRRNKEFVKIQADMK
SQVSIDYSNCTPVIETMLVSIQHDEDYNIEYFNKKVSTIMDQIAIKYGLNTDFKKIINSSGRFVIGGPIGDTGLTGRKII
VDTYGGIGHHGGGAFSGKDPTKVDRSASYFAR*IAKNVVAAKLAKQCEIQLAFAIGEPQPVAMFVNTFKTNLVDEKKIFE
AINKSFNFDIKTFINDLEL*TTKYLPVATYGHFGRDDLNLS*EKLNKVQDLIKYSK

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=366, Percent_Identity=45.9016393442623, Blast_Score=278, Evalue=7e-75,
Organism=Homo sapiens, GI4557737, Length=368, Percent_Identity=44.8369565217391, Blast_Score=275, Evalue=7e-74,
Organism=Escherichia coli, GI1789311, Length=376, Percent_Identity=44.9468085106383, Blast_Score=305, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI25145633, Length=391, Percent_Identity=43.9897698209719, Blast_Score=283, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17538498, Length=379, Percent_Identity=43.2717678100264, Blast_Score=278, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17538494, Length=376, Percent_Identity=43.3510638297872, Blast_Score=278, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17551082, Length=377, Percent_Identity=41.9098143236074, Blast_Score=264, Evalue=6e-71,
Organism=Caenorhabditis elegans, GI32565905, Length=368, Percent_Identity=40.4891304347826, Blast_Score=250, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI25145635, Length=342, Percent_Identity=41.812865497076, Blast_Score=225, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI32565911, Length=330, Percent_Identity=41.2121212121212, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI32565903, Length=293, Percent_Identity=41.6382252559727, Blast_Score=207, Evalue=7e-54,
Organism=Caenorhabditis elegans, GI32565909, Length=81, Percent_Identity=50.6172839506173, Blast_Score=85, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323209, Length=363, Percent_Identity=46.2809917355372, Blast_Score=290, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6320710, Length=378, Percent_Identity=44.7089947089947, Blast_Score=280, Evalue=3e-76,
Organism=Drosophila melanogaster, GI45552159, Length=373, Percent_Identity=45.8445040214477, Blast_Score=271, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24580529, Length=373, Percent_Identity=45.8445040214477, Blast_Score=271, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24580537, Length=373, Percent_Identity=45.8445040214477, Blast_Score=271, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24580533, Length=373, Percent_Identity=45.8445040214477, Blast_Score=271, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24580535, Length=373, Percent_Identity=45.8445040214477, Blast_Score=271, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24580531, Length=373, Percent_Identity=45.8445040214477, Blast_Score=271, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24580527, Length=373, Percent_Identity=45.0402144772118, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24580525, Length=373, Percent_Identity=45.0402144772118, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24580541, Length=361, Percent_Identity=44.0443213296399, Blast_Score=251, Evalue=7e-67,
Organism=Drosophila melanogaster, GI24580539, Length=118, Percent_Identity=45.7627118644068, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 41172; Mature: 41172

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYKKIITSESVGAGHPDKICDQISDAILDECLAQDKNSRVACEVLACNRLIVIAGEITT
CCCHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEEECHHH
HAYVDVVKTAEIIKPLGYNENDFTIISNVNKQSVDIAQSVNKANKDLIGAGDQGIVFGYA
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
CDETPQYMPLTTVLAHELLKEIEKQRRNKEFVKIQADMKSQVSIDYSNCTPVIETMLVSI
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHE
QHDEDYNIEYFNKKVSTIMDQIAIKYGLNTDFKKIINSSGRFVIGGPIGDTGLTGRKIIV
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEECCCCCCCCCCCEEEE
DTYGGIGHHGGGAFSGKDPTKVDRSASYFARIAKNVVAAKLAKQCEIQLAFAIGEPQPVA
ECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHH
MFVNTFKTNLVDEKKIFEAINKSFNFDIKTFINDLELTTKYLPVATYGHFGRDDLNLSEK
HHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHH
LNKVQDLIKYSK
HHHHHHHHHCCC
>Mature Secondary Structure
MKYKKIITSESVGAGHPDKICDQISDAILDECLAQDKNSRVACEVLACNRLIVIAGEITT
CCCHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEEECHHH
HAYVDVVKTAEIIKPLGYNENDFTIISNVNKQSVDIAQSVNKANKDLIGAGDQGIVFGYA
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
CDETPQYMPLTTVLAHELLKEIEKQRRNKEFVKIQADMKSQVSIDYSNCTPVIETMLVSI
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHE
QHDEDYNIEYFNKKVSTIMDQIAIKYGLNTDFKKIINSSGRFVIGGPIGDTGLTGRKIIV
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEECCCCCCCCCCCEEEE
DTYGGIGHHGGGAFSGKDPTKVDRSASYFARIAKNVVAAKLAKQCEIQLAFAIGEPQPVA
ECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHH
MFVNTFKTNLVDEKKIFEAINKSFNFDIKTFINDLELTTKYLPVATYGHFGRDDLNLSEK
HHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHH
LNKVQDLIKYSK
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11353084 [H]