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The map label for this gene is nusA

Identifier: 15595145

GI number: 15595145

Start: 843003

End: 844451

Strand: Direct

Name: nusA

Synonym: BB0800

Alternate gene names: 15595145

Gene position: 843003-844451 (Clockwise)

Preceding gene: 15595144

Following gene: 15595146

Centisome position: 92.56

GC content: 31.54

Gene sequence:

>1449_bases
ATGATAAAGGGCACGGGGCATATGATTGTAAATATTGCAAATGATCGTGGCATGAGTATAGATTCTATTAGAAAAACAAT
TAAAGAATCAGTATTAATAGCTTATAAGAAGTATTTTGGAAGCAATGAGAATGCTTTTATTAAGTTTGATGATGATACGG
GAGATTTGTCTGTTTATGCAAAAAAGAAAATTGTAAAAGAGGTAAAAGATTCTTTGCTTGAAATATTAGAAAAAGATATC
TCAAAGGAAAATATTGTAGAAGGTGATTATGCTTACATTGAAATTAATCCCAAAGTTTTTGATAGGCTTTCTATTCAGGT
TGCAAAACAAAGAACCAAAAATGACCTGCAAGGAATTGAGGATAATGAGATTTTATCAGAATTTAAAAGCAAGTTGAATA
AGGTTGTTATTGGGTACGTTCAACAAAATAGAAATGGTGATCTTTATGTTAATCTTGGTAATACGGATGGTATAATTCCC
AAGAAATATCAATCGCCAAGAGAAGTTTATAACCTTAATGATAAGATTAGAGTTTTAGTTTATAATGTCAAAAAGGGTAA
AAATGGTATTGAAGTTATTCTTTCTAGGACCCATCCAAAGTTTATTGAAGAGCTTTTAGCGCTTGAAATTCCAGAAATTG
AAGAAGGTATTATTAAGATTCATAAAATAGTTCGCGATCCTGGTTACAGAATCAAAGTCGCTGTTTATTCTGAAAAAGAA
GAGATTGATCCTGTTGGTCCTTGTATAGGACAAAAAGGAGTCAGAATTCAATCTATAATTAAGGAACTTGAAGGAGAAAA
AATTGATATTATTCCCTATAGTAAAGATATTAAAGAATTTATAAAGGATTCTTTGACTCCTTCAAAGATAGAACATGTTT
ATATTCTTGACGAGGATTTACATAAAGCTTTAGTGGTTGTTAGTGATGACCAGCTTTCTCTTGCTATAGGTAAAATGGGT
CAAAATGTTAGACTTGCTAATAGACTTCTTGACTGGGCTATTGATGTTAAAACTAGCAGTCAATTTGCAGAAATGAAGGC
TAATTCGGAGTTTAAGCAAGAAACACTCGAAATGTTTGATAAAGTTATGCAAGATGTTGTTGAGGAGGAGCAATTTGAAG
AGATTAGTAAAATAAGTGATCTTAAATTGCTTGATCCTTCTGTGATTTCTAATTTATCAAAAGAGGGGTTTGATGATATT
AACAATTTTTTACAAGCAGATGAGGGAGTGCTTTTTAATCTTGGTGTAAGTTATGAAAAACAAGAAGAAATTAACAAAAT
ATTAAAAGAGGGGATGATAATAATTGCTAATGACAATGATGAGTCTATGGAAAAGGTAGAAGAAGATGAAGAGCTTCTTT
GTCCTGAATGCGGTGTTGTTATTAATGAAAATATGACCTCCTGTCCGGGTTGTAAAATAGGGCTTAGTTTTGAGTTTGAG
GAGGAGTAG

Upstream 100 bases:

>100_bases
TCAAAGCCAAAAAGTTTTATTTTTAAAACAGATAGCAAAGAAGTAAATGTTTTTTATAGTGATGTTAAAAAGGCTAGATT
AGTTTAAGGAGGAAGTTTGG

Downstream 100 bases:

>100_bases
GTTTGTCGAAAAATATTGATGATATTAAAAATGAAGATGGCAAAAAAGTTAAGATTATTAAGTTGAAAAAGAAGGTAGTA
AAAATTGTAACATATAATGA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MIKGTGHMIVNIANDRGMSIDSIRKTIKESVLIAYKKYFGSNENAFIKFDDDTGDLSVYAKKKIVKEVKDSLLEILEKDI
SKENIVEGDYAYIEINPKVFDRLSIQVAKQRTKNDLQGIEDNEILSEFKSKLNKVVIGYVQQNRNGDLYVNLGNTDGIIP
KKYQSPREVYNLNDKIRVLVYNVKKGKNGIEVILSRTHPKFIEELLALEIPEIEEGIIKIHKIVRDPGYRIKVAVYSEKE
EIDPVGPCIGQKGVRIQSIIKELEGEKIDIIPYSKDIKEFIKDSLTPSKIEHVYILDEDLHKALVVVSDDQLSLAIGKMG
QNVRLANRLLDWAIDVKTSSQFAEMKANSEFKQETLEMFDKVMQDVVEEEQFEEISKISDLKLLDPSVISNLSKEGFDDI
NNFLQADEGVLFNLGVSYEKQEEINKILKEGMIIIANDNDESMEKVEEDEELLCPECGVVINENMTSCPGCKIGLSFEFE
EE

Sequences:

>Translated_482_residues
MIKGTGHMIVNIANDRGMSIDSIRKTIKESVLIAYKKYFGSNENAFIKFDDDTGDLSVYAKKKIVKEVKDSLLEILEKDI
SKENIVEGDYAYIEINPKVFDRLSIQVAKQRTKNDLQGIEDNEILSEFKSKLNKVVIGYVQQNRNGDLYVNLGNTDGIIP
KKYQSPREVYNLNDKIRVLVYNVKKGKNGIEVILSRTHPKFIEELLALEIPEIEEGIIKIHKIVRDPGYRIKVAVYSEKE
EIDPVGPCIGQKGVRIQSIIKELEGEKIDIIPYSKDIKEFIKDSLTPSKIEHVYILDEDLHKALVVVSDDQLSLAIGKMG
QNVRLANRLLDWAIDVKTSSQFAEMKANSEFKQETLEMFDKVMQDVVEEEQFEEISKISDLKLLDPSVISNLSKEGFDDI
NNFLQADEGVLFNLGVSYEKQEEINKILKEGMIIIANDNDESMEKVEEDEELLCPECGVVINENMTSCPGCKIGLSFEFE
EE
>Mature_482_residues
MIKGTGHMIVNIANDRGMSIDSIRKTIKESVLIAYKKYFGSNENAFIKFDDDTGDLSVYAKKKIVKEVKDSLLEILEKDI
SKENIVEGDYAYIEINPKVFDRLSIQVAKQRTKNDLQGIEDNEILSEFKSKLNKVVIGYVQQNRNGDLYVNLGNTDGIIP
KKYQSPREVYNLNDKIRVLVYNVKKGKNGIEVILSRTHPKFIEELLALEIPEIEEGIIKIHKIVRDPGYRIKVAVYSEKE
EIDPVGPCIGQKGVRIQSIIKELEGEKIDIIPYSKDIKEFIKDSLTPSKIEHVYILDEDLHKALVVVSDDQLSLAIGKMG
QNVRLANRLLDWAIDVKTSSQFAEMKANSEFKQETLEMFDKVMQDVVEEEQFEEISKISDLKLLDPSVISNLSKEGFDDI
NNFLQADEGVLFNLGVSYEKQEEINKILKEGMIIIANDNDESMEKVEEDEELLCPECGVVINENMTSCPGCKIGLSFEFE
EE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Escherichia coli, GI1789560, Length=412, Percent_Identity=27.4271844660194, Blast_Score=191, Evalue=1e-49,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NUSA_BORBU (O51740)

Other databases:

- EMBL:   AE000783
- PIR:   G70199
- RefSeq:   NP_212934.1
- ProteinModelPortal:   O51740
- SMR:   O51740
- EnsemblBacteria:   EBBORT00000008476
- GeneID:   1195658
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0800
- NMPDR:   fig|224326.1.peg.1184
- TIGR:   BB_0800
- GeneTree:   EBGT00050000007065
- HOGENOM:   HBG529269
- OMA:   RSKISVY
- ProtClustDB:   PRK09202
- BioCyc:   BBUR224326:BB_0800-MONOMER
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213
- Gene3D:   G3DSA:3.30.300.20
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.30.1480.10
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR01953

Pfam domain/function: PF08529 NusA_N; PF00575 S1; SSF54814 KH_prok; SSF50249 Nucleic_acid_OB; SSF69705 TF_NusA_N

EC number: NA

Molecular weight: Translated: 54817; Mature: 54817

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKGTGHMIVNIANDRGMSIDSIRKTIKESVLIAYKKYFGSNENAFIKFDDDTGDLSVYA
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEHHH
KKKIVKEVKDSLLEILEKDISKENIVEGDYAYIEINPKVFDRLSIQVAKQRTKNDLQGIE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCC
DNEILSEFKSKLNKVVIGYVQQNRNGDLYVNLGNTDGIIPKKYQSPREVYNLNDKIRVLV
HHHHHHHHHHHHHHEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCEEEEEE
YNVKKGKNGIEVILSRTHPKFIEELLALEIPEIEEGIIKIHKIVRDPGYRIKVAVYSEKE
EEECCCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCC
EIDPVGPCIGQKGVRIQSIIKELEGEKIDIIPYSKDIKEFIKDSLTPSKIEHVYILDEDL
CCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCHHHCCEEEEECCCC
HKALVVVSDDQLSLAIGKMGQNVRLANRLLDWAIDVKTSSQFAEMKANSEFKQETLEMFD
CCEEEEEECCCEEEEHHHCCCCHHHHHHHHHHHEECCCCHHHHHHHCCCHHHHHHHHHHH
KVMQDVVEEEQFEEISKISDLKLLDPSVISNLSKEGFDDINNFLQADEGVLFNLGVSYEK
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCCH
QEEINKILKEGMIIIANDNDESMEKVEEDEELLCPECGVVINENMTSCPGCKIGLSFEFE
HHHHHHHHHCCEEEEECCCCHHHHHHCCCHHHCCCCCCEEECCCCCCCCCCEEEEEEEEC
EE
CC
>Mature Secondary Structure
MIKGTGHMIVNIANDRGMSIDSIRKTIKESVLIAYKKYFGSNENAFIKFDDDTGDLSVYA
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEHHH
KKKIVKEVKDSLLEILEKDISKENIVEGDYAYIEINPKVFDRLSIQVAKQRTKNDLQGIE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCC
DNEILSEFKSKLNKVVIGYVQQNRNGDLYVNLGNTDGIIPKKYQSPREVYNLNDKIRVLV
HHHHHHHHHHHHHHEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCEEEEEE
YNVKKGKNGIEVILSRTHPKFIEELLALEIPEIEEGIIKIHKIVRDPGYRIKVAVYSEKE
EEECCCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCC
EIDPVGPCIGQKGVRIQSIIKELEGEKIDIIPYSKDIKEFIKDSLTPSKIEHVYILDEDL
CCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCHHHCCEEEEECCCC
HKALVVVSDDQLSLAIGKMGQNVRLANRLLDWAIDVKTSSQFAEMKANSEFKQETLEMFD
CCEEEEEECCCEEEEHHHCCCCHHHHHHHHHHHEECCCCHHHHHHHCCCHHHHHHHHHHH
KVMQDVVEEEQFEEISKISDLKLLDPSVISNLSKEGFDDINNFLQADEGVLFNLGVSYEK
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCCH
QEEINKILKEGMIIIANDNDESMEKVEEDEELLCPECGVVINENMTSCPGCKIGLSFEFE
HHHHHHHHHCCEEEEECCCCHHHHHHCCCHHHCCCCCCEEECCCCCCCCCCEEEEEEEEC
EE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685