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The map label for this gene is tig

Identifier: 15594955

GI number: 15594955

Start: 636593

End: 637957

Strand: Direct

Name: tig

Synonym: BB0610

Alternate gene names: 15594955

Gene position: 636593-637957 (Clockwise)

Preceding gene: 15594953

Following gene: 15594956

Centisome position: 69.9

GC content: 27.25

Gene sequence:

>1365_bases
GTGATTTTGAGTAAAGATATTAAGCTTCTTCCAGGTTCAAAAGTTGAGGTTGTCATTAGAGTTTCAAAAAACGTTATTCA
GGAAAAATACAATTCATTATTGCAAGATTATTCTTCCCGACTTAAGATTCAAGGCTTTAGAATTGGAAAAGTTCCTATTA
ATGTTATTGAGAATAAATATTCTGAGGGTTTAAAAGCTACCGTTTTAGAAGAAGTGATTAATAATTCTTTTAAAGAATTT
TTCAAAGAAGAGTCCAAAATACCTTTAAGTTATGCTACTCCTACTGTAAAGGAAAAAAATTTAAAATTAAATCTTGACAA
AGATTTTGAATTTACTTTTACATATGAGACTTATCCTGAATTTAAAATTCCAAGTTTTGATGAGATTGATATTAAAGTGG
AGATCCCTGAAGTTTTTATTGATGATTCTGATATTGATGATGAGATTAAGAATCTTCAAATAGAAAATTCAATTATCATT
GAAGATGAGGAAGGAGTTGTCAAGGAAGATAGCATTGTTAAAGTGGATTTTGTTGAGCTTGATGACCTTTCAAATGAGAT
AGTATCAACAAAAAGACAAGGCTTTGTTTTTACGGTTGGAAAATCTGAGACCTATTATGATTTTGACAAAGATGTAATTG
GTATGAGAATAAATGAAGAGAGAGTTATAGAAAAATCTTATATTGCAGATTACAAATTTGAAGAACTTGCGGGTTCTTCA
AGAAAATTGAAGATTAAGATTAAGAGTATTAAAAAAAGAGATCTCCCCTTAATAGATGATGAGTTTGCACAGGATATTAG
TGATAAATATAATACACTAGATGATCTTAAAAATTTTATAAGATCTAGTCTTTTAAACATTGTTGAAGAAAAAAAAGAAA
CTTTAAAGCTAAATAAATTTTTTTCTACTATTTCTGAAAAATTAGAAATAGATATTCCCCATTCAATGATTGAGGCTGAA
ATTGAAATTGCTTTTAAGGATGCTAAAAGGCAAAATAAAAATAATATGAGCCTTGAAGAGTTTAAAAGTATTTTTTATTC
TTCAGGATATATTGGTGGTGATAATTTAAAAGATGAGATTCTTGGGAATTTGAAATCTAAACTGATAATGCAAAAAATGG
TAGATTTAGACCCAATTAAAGTTACTGAAAGCGATGTTGAAGATGAAATGGCCAGGCAGTCTAAGAATTTAGGTGTAAGT
TATGAGGAGATTAAAAAATTTTATGAAGATCAAAATTTGATTTCTTATTTAAAGGATGATATTAAAAGGGAAAGAGCTAA
GAAAAAAATTTTGGAAAATCTTAAAGAGGTAAAGGGTAAAAAACTAAATTTTAGAGATTTTGTTAATTATAAAATTTGTG
AGTAA

Upstream 100 bases:

>100_bases
CAGGGGTAGAGTGTCACCTTGCCAAGGTGGAAGTCGCGGGTTCAAATCCCGTCTTTCGCTTTTTAATAATTATCAATAAA
ATTTAATTGAGGCATTAACA

Downstream 100 bases:

>100_bases
GAAAAATGGAGTTTATGCATAATTTAATACCCACTGTAATAGAGAATACGGGAAATTATGAGAGAGTATTTGATATATAT
TCAAGATTACTTAGGGAGCG

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MILSKDIKLLPGSKVEVVIRVSKNVIQEKYNSLLQDYSSRLKIQGFRIGKVPINVIENKYSEGLKATVLEEVINNSFKEF
FKEESKIPLSYATPTVKEKNLKLNLDKDFEFTFTYETYPEFKIPSFDEIDIKVEIPEVFIDDSDIDDEIKNLQIENSIII
EDEEGVVKEDSIVKVDFVELDDLSNEIVSTKRQGFVFTVGKSETYYDFDKDVIGMRINEERVIEKSYIADYKFEELAGSS
RKLKIKIKSIKKRDLPLIDDEFAQDISDKYNTLDDLKNFIRSSLLNIVEEKKETLKLNKFFSTISEKLEIDIPHSMIEAE
IEIAFKDAKRQNKNNMSLEEFKSIFYSSGYIGGDNLKDEILGNLKSKLIMQKMVDLDPIKVTESDVEDEMARQSKNLGVS
YEEIKKFYEDQNLISYLKDDIKRERAKKKILENLKEVKGKKLNFRDFVNYKICE

Sequences:

>Translated_454_residues
MILSKDIKLLPGSKVEVVIRVSKNVIQEKYNSLLQDYSSRLKIQGFRIGKVPINVIENKYSEGLKATVLEEVINNSFKEF
FKEESKIPLSYATPTVKEKNLKLNLDKDFEFTFTYETYPEFKIPSFDEIDIKVEIPEVFIDDSDIDDEIKNLQIENSIII
EDEEGVVKEDSIVKVDFVELDDLSNEIVSTKRQGFVFTVGKSETYYDFDKDVIGMRINEERVIEKSYIADYKFEELAGSS
RKLKIKIKSIKKRDLPLIDDEFAQDISDKYNTLDDLKNFIRSSLLNIVEEKKETLKLNKFFSTISEKLEIDIPHSMIEAE
IEIAFKDAKRQNKNNMSLEEFKSIFYSSGYIGGDNLKDEILGNLKSKLIMQKMVDLDPIKVTESDVEDEMARQSKNLGVS
YEEIKKFYEDQNLISYLKDDIKRERAKKKILENLKEVKGKKLNFRDFVNYKICE
>Mature_454_residues
MILSKDIKLLPGSKVEVVIRVSKNVIQEKYNSLLQDYSSRLKIQGFRIGKVPINVIENKYSEGLKATVLEEVINNSFKEF
FKEESKIPLSYATPTVKEKNLKLNLDKDFEFTFTYETYPEFKIPSFDEIDIKVEIPEVFIDDSDIDDEIKNLQIENSIII
EDEEGVVKEDSIVKVDFVELDDLSNEIVSTKRQGFVFTVGKSETYYDFDKDVIGMRINEERVIEKSYIADYKFEELAGSS
RKLKIKIKSIKKRDLPLIDDEFAQDISDKYNTLDDLKNFIRSSLLNIVEEKKETLKLNKFFSTISEKLEIDIPHSMIEAE
IEIAFKDAKRQNKNNMSLEEFKSIFYSSGYIGGDNLKDEILGNLKSKLIMQKMVDLDPIKVTESDVEDEMARQSKNLGVS
YEEIKKFYEDQNLISYLKDDIKRERAKKKILENLKEVKGKKLNFRDFVNYKICE

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=335, Percent_Identity=26.2686567164179, Blast_Score=115, Evalue=7e-27,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_BORBU (O51555)

Other databases:

- EMBL:   AE000783
- PIR:   A70176
- RefSeq:   NP_212744.1
- ProteinModelPortal:   O51555
- SMR:   O51555
- EnsemblBacteria:   EBBORT00000008921
- GeneID:   1195459
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0610
- NMPDR:   fig|224326.1.peg.994
- TIGR:   BB_0610
- GeneTree:   EBGT00050000006990
- HOGENOM:   HBG352938
- OMA:   TERENIE
- ProtClustDB:   PRK01490
- BioCyc:   BBUR224326:BB_0610-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 52906; Mature: 52906

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILSKDIKLLPGSKVEVVIRVSKNVIQEKYNSLLQDYSSRLKIQGFRIGKVPINVIENKY
CCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCHHHHHHHH
SEGLKATVLEEVINNSFKEFFKEESKIPLSYATPTVKEKNLKLNLDKDFEFTFTYETYPE
HCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEEECCCCEEEEEEEECCCC
FKIPSFDEIDIKVEIPEVFIDDSDIDDEIKNLQIENSIIIEDEEGVVKEDSIVKVDFVEL
CCCCCCCCEEEEEECCEEEECCCCCCHHHHCEEECCEEEEECCCCCCCCCCEEEEEEEEH
DDLSNEIVSTKRQGFVFTVGKSETYYDFDKDVIGMRINEERVIEKSYIADYKFEELAGSS
HHHHHHHHHHCCCCEEEEECCCCCEECCCCCEEEEEECHHHHHHHHHHCCCCHHHHCCCC
RKLKIKIKSIKKRDLPLIDDEFAQDISDKYNTLDDLKNFIRSSLLNIVEEKKETLKLNKF
CEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSTISEKLEIDIPHSMIEAEIEIAFKDAKRQNKNNMSLEEFKSIFYSSGYIGGDNLKDEI
HHHHHHHEEECCCHHHHHHHHHEEEHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCHHHH
LGNLKSKLIMQKMVDLDPIKVTESDVEDEMARQSKNLGVSYEEIKKFYEDQNLISYLKDD
HHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHH
IKRERAKKKILENLKEVKGKKLNFRDFVNYKICE
HHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEECC
>Mature Secondary Structure
MILSKDIKLLPGSKVEVVIRVSKNVIQEKYNSLLQDYSSRLKIQGFRIGKVPINVIENKY
CCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCHHHHHHHH
SEGLKATVLEEVINNSFKEFFKEESKIPLSYATPTVKEKNLKLNLDKDFEFTFTYETYPE
HCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEEECCCCEEEEEEEECCCC
FKIPSFDEIDIKVEIPEVFIDDSDIDDEIKNLQIENSIIIEDEEGVVKEDSIVKVDFVEL
CCCCCCCCEEEEEECCEEEECCCCCCHHHHCEEECCEEEEECCCCCCCCCCEEEEEEEEH
DDLSNEIVSTKRQGFVFTVGKSETYYDFDKDVIGMRINEERVIEKSYIADYKFEELAGSS
HHHHHHHHHHCCCCEEEEECCCCCEECCCCCEEEEEECHHHHHHHHHHCCCCHHHHCCCC
RKLKIKIKSIKKRDLPLIDDEFAQDISDKYNTLDDLKNFIRSSLLNIVEEKKETLKLNKF
CEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSTISEKLEIDIPHSMIEAEIEIAFKDAKRQNKNNMSLEEFKSIFYSSGYIGGDNLKDEI
HHHHHHHEEECCCHHHHHHHHHEEEHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCHHHH
LGNLKSKLIMQKMVDLDPIKVTESDVEDEMARQSKNLGVSYEEIKKFYEDQNLISYLKDD
HHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHH
IKRERAKKKILENLKEVKGKKLNFRDFVNYKICE
HHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685