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The map label for this gene is pcrA [H]

Identifier: 15594952

GI number: 15594952

Start: 632485

End: 634464

Strand: Direct

Name: pcrA [H]

Synonym: BB0607

Alternate gene names: 15594952

Gene position: 632485-634464 (Clockwise)

Preceding gene: 15594951

Following gene: 15594953

Centisome position: 69.45

GC content: 28.03

Gene sequence:

>1980_bases
TTGTCTAATGATGATTTTTTAAAAAATTCTCTCAATCAATTTCAATATGAAGCAGTTACTACTATTGAAGGCGCTCTTTT
AATTATTGCTGGTGCTGGTAGTGGAAAAACAAGGGTTGTTACTCACAGAATAGCGTATTTGCTTTTAAAAGGCATTGCTC
AGAGGGAAATTTTAGCCTTGACTTTTACCAACAAGGCTGCTAATGAAATGAAAGACAGAATTAAAAAAATTTTAAAAAGT
CCTCTTAGTAATCTTATGGTTTCAACTTTCCATGCTTTTGGGCTTTACTTTTTAAAAGAAAATTATAAATTGTTAGGATA
TAGAAAAAACTTTAGTATTTATGATGACAATGATAGAATCTCTCTTCTTAAAGAGATTTTACTTGATGAGGGTCTTTTTA
ATAAAAAAGTTTCTTTAAATTCGCTTAGCAATGTTATTTCACTTTTAAAAAACGGTATTCTCACTCTTAATGATTTAAAG
GAAGAAGATATAAATATTTACAGGCTTTATGAAGAGAGGTTAAGGCTTTATAATTCTTTTGATTTTGATGATTTGATTTT
AAAGCCAAAAGAATTGTTAAGTAATAATTCTGATATTAGAAATAAATATTCTAAAAGATATAAATATGTTTTGATTGACG
AGTTTCAAGATACTTCTTTGATTCAATATAATTTTATTCGCCTTTTGATAAATCATAGTAATTTGTGTTGTGTTGGGGAT
GATGATCAGTCGATATATTCTTGGCGCGGGGCTAATTATAACAACATGTTGCAATTTGAAAAAGATTACAACGTTAAAGA
AATAAAGCTTGAACAAAATTATCGTTCTGCAAAAAATATTTTAGATGTTGCCAATTCTGTGATTTTAAATAATAAAAATA
GAAAAGAAAAGACCTTGTGGTCCTCAAAGATGTGTAGCAAAGTTATAGATGTTTTTATATTTGAGGATGAAATTCAAGAA
TCTGAGTTTGTTGCAAATAGAATCATGCAGCTTTCAAGGCTAGAGGATTTTAAGTCTAAAAAGATAGGGGTTCTTATGAG
AACCAATGCTCTTTTTAAAAACGTTGAAATGATTTTTAGAAGACAAGGCATAAAATATAAAGTTTCAGGGGGAACATCTT
TTTTCCAGAGAAAAGAAATAAAAGATATTATTTCTTATTTGAATGTAATAATAAATCCTAAAAGTGATTATGATCTTCTT
AGAATCATTAATGTTCCAAGAAGGGGGATTGGCAAGGAATATTTGAAAAAAATTAGAGATATAGCAGACAAGAAGGGTTG
TTGCATTTATGATGCTCTTTGTGACATTACTTTTTCTTTTACAAATATTTCTAGTTATGACAAGGCTTTAAATAAGCAGG
TCATTGAAAGCATAGAAGATTTTGTATCTTTTATTGAAGAGTATCAATATAAGTTTAGTATAACAAAAAACACGTATTCC
AATATAATCAAAGAAATGATAGAAAGTATTGAATATTGGGGATTTTTAGTTAGTGAAAATCCCAATTCAATCAAAGTGGC
TGAATATAAGTATCAAAATATAGAAGGGTTTTTAAGCATAATTAAAAATTGGGAATCCAAACAATTTGGTGAGTTGAGAG
ATTTAAGTAGCTTTTTAAATTACATAGTTCTTCAATCTAACGAAGTAAGTGAGGAAGCTGAAAATATTAATATTAATTTA
ATGACAGTACATTCTGCTAAAGGTTTAGAATTTGATTATGTATTTTTTATTGCTGTTGAAGACAATATTATTCCTCATCA
TAGAATCATTGAAGATAGTGAGGTTGACTTAGAAGAAGAGAGGCGAGTTTTTTATGTTGCGTTAACTCGCGCAAAAGATT
CTCTTGTTATTACTATGGCTAATAAGCGTAAAAAAGATAGGCAAATTTTTGATCAGTTGCCTTCAAGATTTATTTCAGAA
ATTCCTAAAGAATTTTTAAATTTTAATTATTATGCAGATTTTGTTGAAAACAAAGCTTAA

Upstream 100 bases:

>100_bases
CTAAAGATTTTGATCAATCTAAGTCGCGAAAAATTTTTGCTTTTCCTGAAAACTTTAAGGTTATTGTGGAATATCCAGAC
GGAACAAAGGTTTTTTAATT

Downstream 100 bases:

>100_bases
CCAATATTATTGAATTTATTGAATGAATTTAAAGATTAATTTAGTATATTATATTATTAATTATAAATTAAATCCAAGTA
TATTTATGGATTTAATTTAA

Product: single-stranded DNA-dependent ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 659; Mature: 658

Protein sequence:

>659_residues
MSNDDFLKNSLNQFQYEAVTTIEGALLIIAGAGSGKTRVVTHRIAYLLLKGIAQREILALTFTNKAANEMKDRIKKILKS
PLSNLMVSTFHAFGLYFLKENYKLLGYRKNFSIYDDNDRISLLKEILLDEGLFNKKVSLNSLSNVISLLKNGILTLNDLK
EEDINIYRLYEERLRLYNSFDFDDLILKPKELLSNNSDIRNKYSKRYKYVLIDEFQDTSLIQYNFIRLLINHSNLCCVGD
DDQSIYSWRGANYNNMLQFEKDYNVKEIKLEQNYRSAKNILDVANSVILNNKNRKEKTLWSSKMCSKVIDVFIFEDEIQE
SEFVANRIMQLSRLEDFKSKKIGVLMRTNALFKNVEMIFRRQGIKYKVSGGTSFFQRKEIKDIISYLNVIINPKSDYDLL
RIINVPRRGIGKEYLKKIRDIADKKGCCIYDALCDITFSFTNISSYDKALNKQVIESIEDFVSFIEEYQYKFSITKNTYS
NIIKEMIESIEYWGFLVSENPNSIKVAEYKYQNIEGFLSIIKNWESKQFGELRDLSSFLNYIVLQSNEVSEEAENININL
MTVHSAKGLEFDYVFFIAVEDNIIPHHRIIEDSEVDLEEERRVFYVALTRAKDSLVITMANKRKKDRQIFDQLPSRFISE
IPKEFLNFNYYADFVENKA

Sequences:

>Translated_659_residues
MSNDDFLKNSLNQFQYEAVTTIEGALLIIAGAGSGKTRVVTHRIAYLLLKGIAQREILALTFTNKAANEMKDRIKKILKS
PLSNLMVSTFHAFGLYFLKENYKLLGYRKNFSIYDDNDRISLLKEILLDEGLFNKKVSLNSLSNVISLLKNGILTLNDLK
EEDINIYRLYEERLRLYNSFDFDDLILKPKELLSNNSDIRNKYSKRYKYVLIDEFQDTSLIQYNFIRLLINHSNLCCVGD
DDQSIYSWRGANYNNMLQFEKDYNVKEIKLEQNYRSAKNILDVANSVILNNKNRKEKTLWSSKMCSKVIDVFIFEDEIQE
SEFVANRIMQLSRLEDFKSKKIGVLMRTNALFKNVEMIFRRQGIKYKVSGGTSFFQRKEIKDIISYLNVIINPKSDYDLL
RIINVPRRGIGKEYLKKIRDIADKKGCCIYDALCDITFSFTNISSYDKALNKQVIESIEDFVSFIEEYQYKFSITKNTYS
NIIKEMIESIEYWGFLVSENPNSIKVAEYKYQNIEGFLSIIKNWESKQFGELRDLSSFLNYIVLQSNEVSEEAENININL
MTVHSAKGLEFDYVFFIAVEDNIIPHHRIIEDSEVDLEEERRVFYVALTRAKDSLVITMANKRKKDRQIFDQLPSRFISE
IPKEFLNFNYYADFVENKA
>Mature_658_residues
SNDDFLKNSLNQFQYEAVTTIEGALLIIAGAGSGKTRVVTHRIAYLLLKGIAQREILALTFTNKAANEMKDRIKKILKSP
LSNLMVSTFHAFGLYFLKENYKLLGYRKNFSIYDDNDRISLLKEILLDEGLFNKKVSLNSLSNVISLLKNGILTLNDLKE
EDINIYRLYEERLRLYNSFDFDDLILKPKELLSNNSDIRNKYSKRYKYVLIDEFQDTSLIQYNFIRLLINHSNLCCVGDD
DQSIYSWRGANYNNMLQFEKDYNVKEIKLEQNYRSAKNILDVANSVILNNKNRKEKTLWSSKMCSKVIDVFIFEDEIQES
EFVANRIMQLSRLEDFKSKKIGVLMRTNALFKNVEMIFRRQGIKYKVSGGTSFFQRKEIKDIISYLNVIINPKSDYDLLR
IINVPRRGIGKEYLKKIRDIADKKGCCIYDALCDITFSFTNISSYDKALNKQVIESIEDFVSFIEEYQYKFSITKNTYSN
IIKEMIESIEYWGFLVSENPNSIKVAEYKYQNIEGFLSIIKNWESKQFGELRDLSSFLNYIVLQSNEVSEEAENININLM
TVHSAKGLEFDYVFFIAVEDNIIPHHRIIEDSEVDLEEERRVFYVALTRAKDSLVITMANKRKKDRQIFDQLPSRFISEI
PKEFLNFNYYADFVENKA

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=652, Percent_Identity=37.1165644171779, Blast_Score=390, Evalue=1e-109,
Organism=Escherichia coli, GI2367296, Length=654, Percent_Identity=33.3333333333333, Blast_Score=345, Evalue=5e-96,
Organism=Escherichia coli, GI1787196, Length=340, Percent_Identity=28.5294117647059, Blast_Score=103, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6322369, Length=715, Percent_Identity=28.1118881118881, Blast_Score=184, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6324477, Length=681, Percent_Identity=23.4948604992658, Blast_Score=103, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77170; Mature: 77039

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDDFLKNSLNQFQYEAVTTIEGALLIIAGAGSGKTRVVTHRIAYLLLKGIAQREILAL
CCCCHHHHHHHHHHHHHHHHEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEE
TFTNKAANEMKDRIKKILKSPLSNLMVSTFHAFGLYFLKENYKLLGYRKNFSIYDDNDRI
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCHH
SLLKEILLDEGLFNKKVSLNSLSNVISLLKNGILTLNDLKEEDINIYRLYEERLRLYNSF
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCC
DFDDLILKPKELLSNNSDIRNKYSKRYKYVLIDEFQDTSLIQYNFIRLLINHSNLCCVGD
CCHHHHCCCHHHHCCCCHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHEECCCCEEEECC
DDQSIYSWRGANYNNMLQFEKDYNVKEIKLEQNYRSAKNILDVANSVILNNKNRKEKTLW
CCCCHHEECCCCCCCCEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
SSKMCSKVIDVFIFEDEIQESEFVANRIMQLSRLEDFKSKKIGVLMRTNALFKNVEMIFR
HHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHH
RQGIKYKVSGGTSFFQRKEIKDIISYLNVIINPKSDYDLLRIINVPRRGIGKEYLKKIRD
HCCCEEEECCCCHHHHHHHHHHHHHHHHHEECCCCCCCHHHHCCCCCCCCCHHHHHHHHH
IADKKGCCIYDALCDITFSFTNISSYDKALNKQVIESIEDFVSFIEEYQYKFSITKNTYS
HHCCCCCEEEEHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHH
NIIKEMIESIEYWGFLVSENPNSIKVAEYKYQNIEGFLSIIKNWESKQFGELRDLSSFLN
HHHHHHHHHHHHCEEEEECCCCEEEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YIVLQSNEVSEEAENININLMTVHSAKGLEFDYVFFIAVEDNIIPHHRIIEDSEVDLEEE
HEEECCCCHHHHHHCCEEEEEEEECCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCHHHC
RRVFYVALTRAKDSLVITMANKRKKDRQIFDQLPSRFISEIPKEFLNFNYYADFVENKA
CEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCC
>Mature Secondary Structure 
SNDDFLKNSLNQFQYEAVTTIEGALLIIAGAGSGKTRVVTHRIAYLLLKGIAQREILAL
CCCHHHHHHHHHHHHHHHHEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEE
TFTNKAANEMKDRIKKILKSPLSNLMVSTFHAFGLYFLKENYKLLGYRKNFSIYDDNDRI
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCHH
SLLKEILLDEGLFNKKVSLNSLSNVISLLKNGILTLNDLKEEDINIYRLYEERLRLYNSF
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCC
DFDDLILKPKELLSNNSDIRNKYSKRYKYVLIDEFQDTSLIQYNFIRLLINHSNLCCVGD
CCHHHHCCCHHHHCCCCHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHEECCCCEEEECC
DDQSIYSWRGANYNNMLQFEKDYNVKEIKLEQNYRSAKNILDVANSVILNNKNRKEKTLW
CCCCHHEECCCCCCCCEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
SSKMCSKVIDVFIFEDEIQESEFVANRIMQLSRLEDFKSKKIGVLMRTNALFKNVEMIFR
HHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHH
RQGIKYKVSGGTSFFQRKEIKDIISYLNVIINPKSDYDLLRIINVPRRGIGKEYLKKIRD
HCCCEEEECCCCHHHHHHHHHHHHHHHHHEECCCCCCCHHHHCCCCCCCCCHHHHHHHHH
IADKKGCCIYDALCDITFSFTNISSYDKALNKQVIESIEDFVSFIEEYQYKFSITKNTYS
HHCCCCCEEEEHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHH
NIIKEMIESIEYWGFLVSENPNSIKVAEYKYQNIEGFLSIIKNWESKQFGELRDLSSFLN
HHHHHHHHHHHHCEEEEECCCCEEEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YIVLQSNEVSEEAENININLMTVHSAKGLEFDYVFFIAVEDNIIPHHRIIEDSEVDLEEE
HEEECCCCHHHHHHCCEEEEEEEECCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCHHHC
RRVFYVALTRAKDSLVITMANKRKKDRQIFDQLPSRFISEIPKEFLNFNYYADFVENKA
CEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]