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The map label for this gene is pheS

Identifier: 15594858

GI number: 15594858

Start: 522990

End: 524576

Strand: Direct

Name: pheS

Synonym: BB0513

Alternate gene names: 15594858

Gene position: 522990-524576 (Clockwise)

Preceding gene: 15594857

Following gene: 15594859

Centisome position: 57.43

GC content: 31.32

Gene sequence:

>1587_bases
TTGAAGGTTATATTTATGAAGGCAGATTTAAATTTAATAAAAACATTGCACCCTCTTGAGATAAAAGTAATTGTAAATAA
TGAAGAAGAGGATGATATTTCTGCTTCAATTATTATTGAAAAATTGGGGTTTAATGAAGGTCAAGCAAATAAAACAATTG
AATGGTTAAATTCTAAAGGGATTATTGAAGAGATTTATAGGAAATTAAATGTATTTTATAAAGCAACAGAAAGGGGTCTT
GGTGCTTTAAGAGATGGTTTTGTTGAAGAAAAAATAATAAATTTAGTATCTCAAAAGGCAGTGTTGGCTTCAAATTTAGC
CTTAGAGCTTGATATTGATGTTAAAGAAGTAAGAAAAGCCTTTGGTAATTTGTTAAAAGAGGGCATTCTTTCTCTTGATT
TAGATAAACAGATTATTATAAATTGTTTAGATGGCACAGAGACTAATTATCAAAAAGTACGTGTTTTGTTGGAAAGAGCA
AAAAATAGCGATCTTCTTAGAGAAAGTTTGACAACCGAAGAGCTTTTATTAATATCAAATTTTGCTAAGAAAAAAGGAGC
AGACTCTGTATTTTTTAAAATAATAGAAAAACTTGATTTAAAGTTTAGATTATCTAGTTTTGGATTGGAAGTTAAAAATT
TTTTAATGAAAAGCAAATTGACTGGAGATGAGCTTACTAAGCTTACTCCCGAAATTTTAAAAAATAAAACGTATGAGAAC
AAAAAATTTAGAGCTTACAATATTCATATTCCATCAGCTAAGACTTTTATTGGGCGTGCCAATTCTTATTTAGATTATAT
TTCTAAAATTAAAGATAAATTAGTAGGTCTTGGCTTTCAAGAGTTTGATGGTCCTTTGGTAGAAACAGAATTTTTTAACA
ACGATGCTCTTTTTATGCCCCAATTTCATCCTTCTCGTGATATTAAAGATGTTTATTACATTTCAGATCCTAGCATGCAA
GAATCTTTGCCAGAGCCTTATTTTTCTAATGTAAAATTAGCCCATGAAACAGGATATGCAACAGGTTCAAGAGGCTGGAG
ATATAGTTTTAGTGAAGATCTCTCTAAGAGATTGGTTTTAAGAACCCATGGTACCGTTCTTTCTGCAAAACAATTAATTA
ATGCCAAAAATCCAAGCAGATATTTTGGAGTTATTAGATGTTTTAGGTATGATCAAGTAGATGCAACTCACGGAGTAGAT
TTTTATCAAACCGAAGGGATTGTTATTGAGGATGGTGTTAGCATTAAAACTTTGCTAGGCCTTCTTGAAATTTTTGCCAA
AGAGCTTGCGGGCGCTACAGAAATTAAATATGTTCCCGCATATTTTCCCTTTACTGAACCTTCGATTGAGATACATGTAA
AGCATCCTGTTCTTGGTTGGTTTGAACTTGGAGGAAGTGGAATTTTTAGACCAGAAGTTACAAAGCCTTTAGGCATTGAT
CTGCCTGTTATTGCTTGGGGAATTGGAATAGATAGAATGGCTTTAATGCACTTAGGTCTAAATGATTTGAGAGATCTATT
TACTTATGATATTGGTGATGTTATTTTAAGACGAGGAAAGATAGATGCCAAAGTTAGAAATTTATAA

Upstream 100 bases:

>100_bases
TGAGGCAGGGATGGAGTGCTGAAGAAATTTCTAGAGCTACCAAATTATCTATTGGGGAAGTTGAGCTTATTTTAGAGCTT
GGTATAAGTAATAAAGGTGA

Downstream 100 bases:

>100_bases
AAATCTTTTTTTAGATAAAATAGGAAAAAATTTTACAAATTTAGAGATTTCAGAATTACTTGAACCATTTAAGGCAGAAT
TTGATGGGTTTGATGAGAGT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MKVIFMKADLNLIKTLHPLEIKVIVNNEEEDDISASIIIEKLGFNEGQANKTIEWLNSKGIIEEIYRKLNVFYKATERGL
GALRDGFVEEKIINLVSQKAVLASNLALELDIDVKEVRKAFGNLLKEGILSLDLDKQIIINCLDGTETNYQKVRVLLERA
KNSDLLRESLTTEELLLISNFAKKKGADSVFFKIIEKLDLKFRLSSFGLEVKNFLMKSKLTGDELTKLTPEILKNKTYEN
KKFRAYNIHIPSAKTFIGRANSYLDYISKIKDKLVGLGFQEFDGPLVETEFFNNDALFMPQFHPSRDIKDVYYISDPSMQ
ESLPEPYFSNVKLAHETGYATGSRGWRYSFSEDLSKRLVLRTHGTVLSAKQLINAKNPSRYFGVIRCFRYDQVDATHGVD
FYQTEGIVIEDGVSIKTLLGLLEIFAKELAGATEIKYVPAYFPFTEPSIEIHVKHPVLGWFELGGSGIFRPEVTKPLGID
LPVIAWGIGIDRMALMHLGLNDLRDLFTYDIGDVILRRGKIDAKVRNL

Sequences:

>Translated_528_residues
MKVIFMKADLNLIKTLHPLEIKVIVNNEEEDDISASIIIEKLGFNEGQANKTIEWLNSKGIIEEIYRKLNVFYKATERGL
GALRDGFVEEKIINLVSQKAVLASNLALELDIDVKEVRKAFGNLLKEGILSLDLDKQIIINCLDGTETNYQKVRVLLERA
KNSDLLRESLTTEELLLISNFAKKKGADSVFFKIIEKLDLKFRLSSFGLEVKNFLMKSKLTGDELTKLTPEILKNKTYEN
KKFRAYNIHIPSAKTFIGRANSYLDYISKIKDKLVGLGFQEFDGPLVETEFFNNDALFMPQFHPSRDIKDVYYISDPSMQ
ESLPEPYFSNVKLAHETGYATGSRGWRYSFSEDLSKRLVLRTHGTVLSAKQLINAKNPSRYFGVIRCFRYDQVDATHGVD
FYQTEGIVIEDGVSIKTLLGLLEIFAKELAGATEIKYVPAYFPFTEPSIEIHVKHPVLGWFELGGSGIFRPEVTKPLGID
LPVIAWGIGIDRMALMHLGLNDLRDLFTYDIGDVILRRGKIDAKVRNL
>Mature_528_residues
MKVIFMKADLNLIKTLHPLEIKVIVNNEEEDDISASIIIEKLGFNEGQANKTIEWLNSKGIIEEIYRKLNVFYKATERGL
GALRDGFVEEKIINLVSQKAVLASNLALELDIDVKEVRKAFGNLLKEGILSLDLDKQIIINCLDGTETNYQKVRVLLERA
KNSDLLRESLTTEELLLISNFAKKKGADSVFFKIIEKLDLKFRLSSFGLEVKNFLMKSKLTGDELTKLTPEILKNKTYEN
KKFRAYNIHIPSAKTFIGRANSYLDYISKIKDKLVGLGFQEFDGPLVETEFFNNDALFMPQFHPSRDIKDVYYISDPSMQ
ESLPEPYFSNVKLAHETGYATGSRGWRYSFSEDLSKRLVLRTHGTVLSAKQLINAKNPSRYFGVIRCFRYDQVDATHGVD
FYQTEGIVIEDGVSIKTLLGLLEIFAKELAGATEIKYVPAYFPFTEPSIEIHVKHPVLGWFELGGSGIFRPEVTKPLGID
LPVIAWGIGIDRMALMHLGLNDLRDLFTYDIGDVILRRGKIDAKVRNL

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=490, Percent_Identity=28.1632653061224, Blast_Score=202, Evalue=6e-52,
Organism=Escherichia coli, GI1788007, Length=269, Percent_Identity=27.1375464684015, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32563657, Length=292, Percent_Identity=36.986301369863, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17508957, Length=292, Percent_Identity=36.986301369863, Blast_Score=206, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6321087, Length=457, Percent_Identity=30.1969365426696, Blast_Score=197, Evalue=4e-51,
Organism=Drosophila melanogaster, GI18858079, Length=372, Percent_Identity=32.5268817204301, Blast_Score=191, Evalue=1e-48,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_BORBU (P94282)

Other databases:

- EMBL:   AE000783
- EMBL:   U82978
- PIR:   H70163
- RefSeq:   NP_212647.1
- ProteinModelPortal:   P94282
- SMR:   P94282
- EnsemblBacteria:   EBBORT00000008705
- GeneID:   1195359
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0513
- NMPDR:   fig|224326.1.peg.897
- TIGR:   BB_0513
- GeneTree:   EBGT00050000007094
- HOGENOM:   HBG497668
- OMA:   WFELGGS
- PhylomeDB:   P94282
- ProtClustDB:   PRK04172
- BioCyc:   BBUR224326:BB_0513-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00282
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR022917
- InterPro:   IPR002319
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF01409 tRNA-synt_2d

EC number: =6.1.1.20

Molecular weight: Translated: 59953; Mature: 59953

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIFMKADLNLIKTLHPLEIKVIVNNEEEDDISASIIIEKLGFNEGQANKTIEWLNSKG
CEEEEEECCCHHHHCCCCEEEEEEECCCCCCCCHHEEEEEECCCCCCCCCCHHHHHCCCC
IIEEIYRKLNVFYKATERGLGALRDGFVEEKIINLVSQKAVLASNLALELDIDVKEVRKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHH
FGNLLKEGILSLDLDKQIIINCLDGTETNYQKVRVLLERAKNSDLLRESLTTEELLLISN
HHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHH
FAKKKGADSVFFKIIEKLDLKFRLSSFGLEVKNFLMKSKLTGDELTKLTPEILKNKTYEN
HHHHCCCHHHHHHHHHHHCCEEEHHHHCHHHHHHHHHHCCCCHHHHHCCHHHHCCCCCCC
KKFRAYNIHIPSAKTFIGRANSYLDYISKIKDKLVGLGFQEFDGPLVETEFFNNDALFMP
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEEECCCEEECC
QFHPSRDIKDVYYISDPSMQESLPEPYFSNVKLAHETGYATGSRGWRYSFSEDLSKRLVL
CCCCCCCCCEEEEECCCCHHHHCCCCCCCCEEEEECCCCCCCCCCEEECHHHHHHHHEEE
RTHGTVLSAKQLINAKNPSRYFGVIRCFRYDQVDATHGVDFYQTEGIVIEDGVSIKTLLG
EECCCHHHHHHHHCCCCCHHHHHHHHHEECCCCCCCCCCCEEECCCEEEECCCCHHHHHH
LLEIFAKELAGATEIKYVPAYFPFTEPSIEIHVKHPVLGWFELGGSGIFRPEVTKPLGID
HHHHHHHHHCCCCEEEEEEEECCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCCC
LPVIAWGIGIDRMALMHLGLNDLRDLFTYDIGDVILRRGKIDAKVRNL
CCHHEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCC
>Mature Secondary Structure
MKVIFMKADLNLIKTLHPLEIKVIVNNEEEDDISASIIIEKLGFNEGQANKTIEWLNSKG
CEEEEEECCCHHHHCCCCEEEEEEECCCCCCCCHHEEEEEECCCCCCCCCCHHHHHCCCC
IIEEIYRKLNVFYKATERGLGALRDGFVEEKIINLVSQKAVLASNLALELDIDVKEVRKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHH
FGNLLKEGILSLDLDKQIIINCLDGTETNYQKVRVLLERAKNSDLLRESLTTEELLLISN
HHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHH
FAKKKGADSVFFKIIEKLDLKFRLSSFGLEVKNFLMKSKLTGDELTKLTPEILKNKTYEN
HHHHCCCHHHHHHHHHHHCCEEEHHHHCHHHHHHHHHHCCCCHHHHHCCHHHHCCCCCCC
KKFRAYNIHIPSAKTFIGRANSYLDYISKIKDKLVGLGFQEFDGPLVETEFFNNDALFMP
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEEECCCEEECC
QFHPSRDIKDVYYISDPSMQESLPEPYFSNVKLAHETGYATGSRGWRYSFSEDLSKRLVL
CCCCCCCCCEEEEECCCCHHHHCCCCCCCCEEEEECCCCCCCCCCEEECHHHHHHHHEEE
RTHGTVLSAKQLINAKNPSRYFGVIRCFRYDQVDATHGVDFYQTEGIVIEDGVSIKTLLG
EECCCHHHHHHHHCCCCCHHHHHHHHHEECCCCCCCCCCCEEECCCEEEECCCCHHHHHH
LLEIFAKELAGATEIKYVPAYFPFTEPSIEIHVKHPVLGWFELGGSGIFRPEVTKPLGID
HHHHHHHHHCCCCEEEEEEEECCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCCC
LPVIAWGIGIDRMALMHLGLNDLRDLFTYDIGDVILRRGKIDAKVRNL
CCHHEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685