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The map label for this gene is eno [H]

Identifier: 15594682

GI number: 15594682

Start: 345063

End: 346364

Strand: Direct

Name: eno [H]

Synonym: BB0337

Alternate gene names: 15594682

Gene position: 345063-346364 (Clockwise)

Preceding gene: 15594680

Following gene: 15594692

Centisome position: 37.89

GC content: 37.25

Gene sequence:

>1302_bases
ATGGGTTTTCACATTTATGAAATCAAAGCCAGACAAATCATTGATTCTAGAGGGAATCCAACAGTTGAAGCTGATGTCAT
TTTAGAAGATGGAACTTACGGAAGAGCTGCCGTACCATCAGGTGCATCAACAGGAATTAACGAGGCTGTTGAGCTTAGAG
ATGGTGATAAGTCTGTATATATGGGAAAAGGGGTTTTAAAGGCAATTGAAAATATAAAAAACATAATTGCCCCAGAACTT
GAAGGTATGAGTGCCTTAAATCAGGTTGCAATCGACAGAAAAATGCTTGAACTTGATGGCACCCCTACAAAAGAAAAGCT
TGGTGCTAATGCAATTTTAGCAGTTTCAATGGCTACAGCTAAAGCTGCTGCAAAGTACCTTGGACTTAGGCCTTATCAAT
ATCTTGGAGCGTACAAAGCCAACATTTTGCCTACACCTATGTGTAATATTATTAATGGCGGTGCACACTCTGACAACTCT
GTTGACTTTCAGGAGTTCATGATAATGCCAATAGGAGCAAAAACATTCAGTGAAGCAATAAGAATGGCAGCAGAGGTTTT
TCATACGCTAAAGGGCATTCTAAGTGGCAAAGGGTATGCAACTTCTGTTGGAGATGAAGGGGGATTTGCTCCAAATTTGA
AATCAAATGAAGAAGCTTGTGAAGTGATTATAGAGGCAATAAAGAAGGCAGGATATGAACCTGGAAAAGACATTGCAATA
GCTCTTGATCCCGCAACATCTGAGCTTTATGATCCAAAAACAAAAAAATACGTACTTAAATGGTCAACAAAAGAAAAACT
TACTTCCGAACAAATGGTTGAATATTGGGCAAAGTGGGTAGAAAAATATCCAATCATTTCAATTGAAGATGGAATGGCTG
AAGAAGATTGGGATGGATGGAAAAAACTTACAGACAAAATTGGAAACAAAATACAACTTGTTGGAGATGATTTATTTGTA
ACAAATACCTCGTTTCTTAAAAAAGGAATTGAAATGGGAGTTGCCAATTCAATCCTTATTAAGGTCAATCAAATTGGAAC
ACTAACAGAAACATTTGAGGCTGTAGAAATGGCTAAAAAAGCGGGTTACACAGCAATAGTCTCTCACAGATCGGGAGAAA
CAGAAGATACAACAATAGCTGATCTTGTAGTAGCTCTTGGAACAGGACAAATCAAAACTGGTTCACTCTCAAGAACAGAT
AGAATAGCAAAATACAATCAACTCATAAGAATAGAGGAAGAATTGGAAACAACTGCTGAATACCACGGTAAGAGCGTCTT
TTATTCTATTAAACAAAAATAA

Upstream 100 bases:

>100_bases
CTCACAATAATAATTCTTTTGGAAAAATACTACACATTTAAACTTTTTAACATTATACTAAAAATATACATTAAAGTAAA
TAATACGAGGAGTACAAAAA

Downstream 100 bases:

>100_bases
ATCAAATCCCTGTAAAAGGGATTTTTTTTAATTAAATAAAAATGTAAAATGTATAAAAAAATAAAATTATCTTTTGGAAA
ATTGAAAACTTTTTCGTGCT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MGFHIYEIKARQIIDSRGNPTVEADVILEDGTYGRAAVPSGASTGINEAVELRDGDKSVYMGKGVLKAIENIKNIIAPEL
EGMSALNQVAIDRKMLELDGTPTKEKLGANAILAVSMATAKAAAKYLGLRPYQYLGAYKANILPTPMCNIINGGAHSDNS
VDFQEFMIMPIGAKTFSEAIRMAAEVFHTLKGILSGKGYATSVGDEGGFAPNLKSNEEACEVIIEAIKKAGYEPGKDIAI
ALDPATSELYDPKTKKYVLKWSTKEKLTSEQMVEYWAKWVEKYPIISIEDGMAEEDWDGWKKLTDKIGNKIQLVGDDLFV
TNTSFLKKGIEMGVANSILIKVNQIGTLTETFEAVEMAKKAGYTAIVSHRSGETEDTTIADLVVALGTGQIKTGSLSRTD
RIAKYNQLIRIEEELETTAEYHGKSVFYSIKQK

Sequences:

>Translated_433_residues
MGFHIYEIKARQIIDSRGNPTVEADVILEDGTYGRAAVPSGASTGINEAVELRDGDKSVYMGKGVLKAIENIKNIIAPEL
EGMSALNQVAIDRKMLELDGTPTKEKLGANAILAVSMATAKAAAKYLGLRPYQYLGAYKANILPTPMCNIINGGAHSDNS
VDFQEFMIMPIGAKTFSEAIRMAAEVFHTLKGILSGKGYATSVGDEGGFAPNLKSNEEACEVIIEAIKKAGYEPGKDIAI
ALDPATSELYDPKTKKYVLKWSTKEKLTSEQMVEYWAKWVEKYPIISIEDGMAEEDWDGWKKLTDKIGNKIQLVGDDLFV
TNTSFLKKGIEMGVANSILIKVNQIGTLTETFEAVEMAKKAGYTAIVSHRSGETEDTTIADLVVALGTGQIKTGSLSRTD
RIAKYNQLIRIEEELETTAEYHGKSVFYSIKQK
>Mature_432_residues
GFHIYEIKARQIIDSRGNPTVEADVILEDGTYGRAAVPSGASTGINEAVELRDGDKSVYMGKGVLKAIENIKNIIAPELE
GMSALNQVAIDRKMLELDGTPTKEKLGANAILAVSMATAKAAAKYLGLRPYQYLGAYKANILPTPMCNIINGGAHSDNSV
DFQEFMIMPIGAKTFSEAIRMAAEVFHTLKGILSGKGYATSVGDEGGFAPNLKSNEEACEVIIEAIKKAGYEPGKDIAIA
LDPATSELYDPKTKKYVLKWSTKEKLTSEQMVEYWAKWVEKYPIISIEDGMAEEDWDGWKKLTDKIGNKIQLVGDDLFVT
NTSFLKKGIEMGVANSILIKVNQIGTLTETFEAVEMAKKAGYTAIVSHRSGETEDTTIADLVVALGTGQIKTGSLSRTDR
IAKYNQLIRIEEELETTAEYHGKSVFYSIKQK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=432, Percent_Identity=51.3888888888889, Blast_Score=417, Evalue=1e-116,
Organism=Homo sapiens, GI301897477, Length=431, Percent_Identity=50.1160092807425, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI301897469, Length=431, Percent_Identity=50.1160092807425, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI4503571, Length=432, Percent_Identity=49.0740740740741, Blast_Score=404, Evalue=1e-112,
Organism=Homo sapiens, GI301897479, Length=429, Percent_Identity=45.4545454545455, Blast_Score=348, Evalue=8e-96,
Organism=Homo sapiens, GI169201331, Length=337, Percent_Identity=26.4094955489614, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI169201757, Length=337, Percent_Identity=26.4094955489614, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI239744207, Length=337, Percent_Identity=26.4094955489614, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1789141, Length=427, Percent_Identity=60.4215456674473, Blast_Score=490, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI71995829, Length=434, Percent_Identity=51.3824884792627, Blast_Score=413, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17536383, Length=434, Percent_Identity=51.3824884792627, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI32563855, Length=193, Percent_Identity=47.6683937823834, Blast_Score=179, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6324974, Length=434, Percent_Identity=48.1566820276498, Blast_Score=386, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324969, Length=434, Percent_Identity=48.1566820276498, Blast_Score=386, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6323985, Length=434, Percent_Identity=47.926267281106, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6321693, Length=442, Percent_Identity=48.1900452488688, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6321968, Length=442, Percent_Identity=47.737556561086, Blast_Score=354, Evalue=2e-98,
Organism=Drosophila melanogaster, GI281360527, Length=433, Percent_Identity=49.6535796766744, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI17137654, Length=433, Percent_Identity=49.6535796766744, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580918, Length=433, Percent_Identity=49.6535796766744, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580916, Length=433, Percent_Identity=49.6535796766744, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580920, Length=433, Percent_Identity=49.6535796766744, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580914, Length=433, Percent_Identity=49.6535796766744, Blast_Score=385, Evalue=1e-107,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 47291; Mature: 47159

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFHIYEIKARQIIDSRGNPTVEADVILEDGTYGRAAVPSGASTGINEAVELRDGDKSVY
CCEEEEEEHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHEEECCCCCEEE
MGKGVLKAIENIKNIIAPELEGMSALNQVAIDRKMLELDGTPTKEKLGANAILAVSMATA
EHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEEHHHHH
KAAAKYLGLRPYQYLGAYKANILPTPMCNIINGGAHSDNSVDFQEFMIMPIGAKTFSEAI
HHHHHHHCCCCHHHHCCHHCCCCCCCHHHHCCCCCCCCCCCCHHHEEEEECCHHHHHHHH
RMAAEVFHTLKGILSGKGYATSVGDEGGFAPNLKSNEEACEVIIEAIKKAGYEPGKDIAI
HHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEE
ALDPATSELYDPKTKKYVLKWSTKEKLTSEQMVEYWAKWVEKYPIISIEDGMAEEDWDGW
EECCCCHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHH
KKLTDKIGNKIQLVGDDLFVTNTSFLKKGIEMGVANSILIKVNQIGTLTETFEAVEMAKK
HHHHHHCCCEEEEEECCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHH
AGYTAIVSHRSGETEDTTIADLVVALGTGQIKTGSLSRTDRIAKYNQLIRIEEELETTAE
CCCEEEEECCCCCCCCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
YHGKSVFYSIKQK
HCCCEEEEEECCC
>Mature Secondary Structure 
GFHIYEIKARQIIDSRGNPTVEADVILEDGTYGRAAVPSGASTGINEAVELRDGDKSVY
CEEEEEEHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHEEECCCCCEEE
MGKGVLKAIENIKNIIAPELEGMSALNQVAIDRKMLELDGTPTKEKLGANAILAVSMATA
EHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEEHHHHH
KAAAKYLGLRPYQYLGAYKANILPTPMCNIINGGAHSDNSVDFQEFMIMPIGAKTFSEAI
HHHHHHHCCCCHHHHCCHHCCCCCCCHHHHCCCCCCCCCCCCHHHEEEEECCHHHHHHHH
RMAAEVFHTLKGILSGKGYATSVGDEGGFAPNLKSNEEACEVIIEAIKKAGYEPGKDIAI
HHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEE
ALDPATSELYDPKTKKYVLKWSTKEKLTSEQMVEYWAKWVEKYPIISIEDGMAEEDWDGW
EECCCCHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHH
KKLTDKIGNKIQLVGDDLFVTNTSFLKKGIEMGVANSILIKVNQIGTLTETFEAVEMAKK
HHHHHHCCCEEEEEECCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHH
AGYTAIVSHRSGETEDTTIADLVVALGTGQIKTGSLSRTDRIAKYNQLIRIEEELETTAE
CCCEEEEECCCCCCCCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
YHGKSVFYSIKQK
HCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA