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The map label for this gene is yvfH

Identifier: 16080472

GI number: 16080472

Start: 3510780

End: 3512471

Strand: Direct

Name: yvfH

Synonym: BSU34190

Alternate gene names: 16080472

Gene position: 3510780-3512471 (Clockwise)

Preceding gene: 16080464

Following gene: 16080474

Centisome position: 83.28

GC content: 50.59

Gene sequence:

>1692_bases
ATGCAATGGACACAGGCATATACGCCTATAGGGGGAAATTTGCTATTATCAGCGCTTGCCGCACTTGTACCGATTATTTT
CTTTTTTTGGGCACTCGCGATTAAACGGATGAAGGGGTATACAGCAGGCTTGGCCACATTGGGGATCGCCCTTATCATTG
CTGTATTGGTTTATCGTATGCCGGCGGAAAAAGCGCTGATGTCCGCGACACAAGGAGCCGTATACGGATTGTTGCCGATC
GGCTGGATCATTGTGACGTCTGTTTTTTTGTATAAAATCACTGTGAAAACGGGACAGTTTGATATCATTCGCAGCTCTGT
TCTCTCGATTACTGATGACCGACGGCTTCAAGCTCTCTTGATCGCTTTTTCATTCGGGGCTTTTTTAGAAGGGGCAGCAG
GCTTCGGAGCGCCGGTCGCCATTTCTGCCGCGCTGCTGGTCGGTCTCGGCTTTAATCCTCTTTATGCTGCGGGGATCTGT
TTGATCGCCAATACGGCGCCGGTTGCCTTTGGGGCAATTGGGATTCCAATTACAGCCGTAGAAGGGCCGACAGGGATTCC
GGCGATGGAGATTTCGCAAATGGTTGGGCGGCAGCTGCCGTTTTTATCCGTATTTATTCCTCTTTATTTAATCATCATCA
TGAGCGGGTTCAGGAAAGCCTTGGAGATCTGGCCGGCGATTCTCGTATCCGGTGTTTCCTTTGCTGTTGTTCAATATCTC
AGTTCCAATTTTTTAGGGCCTGAGCTGCCCGATGTTTTGTCAGCTCTTGTTTCAATGGCTGCGCTTGCTGTGTTTTTGAA
ATGGTGGAAGCCGAAAACAACATTCCGGTTTGCCGGTGAACAAGAGTCGGCCGCCTCAATTGAAACGGCGCGCACAAATC
CGGCGGCGCCTGCATACAGAGGCGGACAGATTTTTAAGGCTTGGTCGCCATTTCTGCTGCTGACGGCGATGATTTCCGTA
TGGGGCATTCCATCTGTCAAATCCGCGCTGACCGGGCATTATGAAGGCTCTGCGGTGTTCTTGAAGTGGCTGAATGCTGT
TGGAGAAAAACTGACTTTTTCCCCCGGTGTGCCTTTTTTGAACAATCAGATTGTCAATGCTGACGGTACGCCGATTGAAG
CGGTTTACAAGTTGGAGGTGCTTGGTTCGGCCGGCACCGCGATATTGATTGCCGCAGTGCTGTCAAAATTCATCACGGCG
ATTTCGTGGAAAGACTGGGGAACCGTTTTTAAAGAAACAGTCCAGGAATTGAAGCTTCCGATTTTGACCATTGCTTCTGT
CGTTGGTTTTGCCTATGTCACCAACTCCTCGGGAATGAGCACGACACTCGGGATGACACTCGCGCTGACAGGTTCGATGT
TTACCTTCTTTTCGCCCGTTCTCGGCTGGCTCGGCGTCTTCATTACCGGCTCGGATACTTCTGCCAACCTGCTGTTCGGC
AACCTGCAGAAAGTCACCGCATTATCAGTCGGCATGGACCCCGTTCTGTCTGTGGCCGCCAACTCTTCAGGCGGTGTCAC
AGGAAAAATGATTTCACCGCAGTCGATAGCGGTGGCGTGCGCCGCGGTAGGACTCGCCGGAAAGGAGTCAGATCTTTTCC
GCTTTACCATTAAACACAGTCTGTTTTTGCTGCTGCTCGTCTGTATCATCACCTTTTTGCAGCATCACGTATTCAGCTGG
ATGATTCCTTAA

Upstream 100 bases:

>100_bases
GCTAGGTAACTGGTCTTATTACCCAGTCATCAGATGATCATATCATCTATAGAAACCGCTTACATATGGCGCCAGTTTAT
GTTTCAAGGAGGGGTTTGGG

Downstream 100 bases:

>100_bases
AAAAATCCTCCCGTCTAGGGAGGATTTTATTTATATCGCTTTCTCGCTGCCGATGACGGAATATTCATTTGATCGCGATA
TTTTGCCACGGTGCGTCTCG

Product: lactate permease

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MQWTQAYTPIGGNLLLSALAALVPIIFFFWALAIKRMKGYTAGLATLGIALIIAVLVYRMPAEKALMSATQGAVYGLLPI
GWIIVTSVFLYKITVKTGQFDIIRSSVLSITDDRRLQALLIAFSFGAFLEGAAGFGAPVAISAALLVGLGFNPLYAAGIC
LIANTAPVAFGAIGIPITAVEGPTGIPAMEISQMVGRQLPFLSVFIPLYLIIIMSGFRKALEIWPAILVSGVSFAVVQYL
SSNFLGPELPDVLSALVSMAALAVFLKWWKPKTTFRFAGEQESAASIETARTNPAAPAYRGGQIFKAWSPFLLLTAMISV
WGIPSVKSALTGHYEGSAVFLKWLNAVGEKLTFSPGVPFLNNQIVNADGTPIEAVYKLEVLGSAGTAILIAAVLSKFITA
ISWKDWGTVFKETVQELKLPILTIASVVGFAYVTNSSGMSTTLGMTLALTGSMFTFFSPVLGWLGVFITGSDTSANLLFG
NLQKVTALSVGMDPVLSVAANSSGGVTGKMISPQSIAVACAAVGLAGKESDLFRFTIKHSLFLLLLVCIITFLQHHVFSW
MIP

Sequences:

>Translated_563_residues
MQWTQAYTPIGGNLLLSALAALVPIIFFFWALAIKRMKGYTAGLATLGIALIIAVLVYRMPAEKALMSATQGAVYGLLPI
GWIIVTSVFLYKITVKTGQFDIIRSSVLSITDDRRLQALLIAFSFGAFLEGAAGFGAPVAISAALLVGLGFNPLYAAGIC
LIANTAPVAFGAIGIPITAVEGPTGIPAMEISQMVGRQLPFLSVFIPLYLIIIMSGFRKALEIWPAILVSGVSFAVVQYL
SSNFLGPELPDVLSALVSMAALAVFLKWWKPKTTFRFAGEQESAASIETARTNPAAPAYRGGQIFKAWSPFLLLTAMISV
WGIPSVKSALTGHYEGSAVFLKWLNAVGEKLTFSPGVPFLNNQIVNADGTPIEAVYKLEVLGSAGTAILIAAVLSKFITA
ISWKDWGTVFKETVQELKLPILTIASVVGFAYVTNSSGMSTTLGMTLALTGSMFTFFSPVLGWLGVFITGSDTSANLLFG
NLQKVTALSVGMDPVLSVAANSSGGVTGKMISPQSIAVACAAVGLAGKESDLFRFTIKHSLFLLLLVCIITFLQHHVFSW
MIP
>Mature_563_residues
MQWTQAYTPIGGNLLLSALAALVPIIFFFWALAIKRMKGYTAGLATLGIALIIAVLVYRMPAEKALMSATQGAVYGLLPI
GWIIVTSVFLYKITVKTGQFDIIRSSVLSITDDRRLQALLIAFSFGAFLEGAAGFGAPVAISAALLVGLGFNPLYAAGIC
LIANTAPVAFGAIGIPITAVEGPTGIPAMEISQMVGRQLPFLSVFIPLYLIIIMSGFRKALEIWPAILVSGVSFAVVQYL
SSNFLGPELPDVLSALVSMAALAVFLKWWKPKTTFRFAGEQESAASIETARTNPAAPAYRGGQIFKAWSPFLLLTAMISV
WGIPSVKSALTGHYEGSAVFLKWLNAVGEKLTFSPGVPFLNNQIVNADGTPIEAVYKLEVLGSAGTAILIAAVLSKFITA
ISWKDWGTVFKETVQELKLPILTIASVVGFAYVTNSSGMSTTLGMTLALTGSMFTFFSPVLGWLGVFITGSDTSANLLFG
NLQKVTALSVGMDPVLSVAANSSGGVTGKMISPQSIAVACAAVGLAGKESDLFRFTIKHSLFLLLLVCIITFLQHHVFSW
MIP

Specific function: Is the principal permease for the uptake of L-lactate in B.subtilis

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family

Homologues:

Organism=Escherichia coli, GI1789347, Length=568, Percent_Identity=55.2816901408451, Blast_Score=567, Evalue=1e-163,
Organism=Escherichia coli, GI1790031, Length=566, Percent_Identity=54.0636042402827, Blast_Score=563, Evalue=1e-161,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LUTP_BACSU (P71067)

Other databases:

- EMBL:   Z94043
- EMBL:   AL009126
- EMBL:   Z71928
- PIR:   A70038
- RefSeq:   NP_391299.1
- ProteinModelPortal:   P71067
- EnsemblBacteria:   EBBACT00000000456
- GeneID:   936322
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34190
- NMPDR:   fig|224308.1.peg.3425
- GenoList:   BSU34190
- GeneTree:   EBGT00050000001890
- HOGENOM:   HBG289840
- OMA:   AVLVYRM
- PhylomeDB:   P71067
- ProtClustDB:   CLSK887902
- BioCyc:   BSUB:BSU34190-MONOMER
- InterPro:   IPR003804
- TIGRFAMs:   TIGR00795

Pfam domain/function: PF02652 Lactate_perm

EC number: NA

Molecular weight: Translated: 59762; Mature: 59762

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1220de5c)-; HASH(0x123f2108)-; HASH(0x12390d20)-; HASH(0x1228f760)-; HASH(0x12643794)-; HASH(0x120a289c)-; HASH(0x127d8efc)-; HASH(0x12777710)-; HASH(0x1272d940)-; HASH(0x12146b60)-; HASH(0x126868e4)-; HASH(0x11dad75c)-; HASH(0x123c2e00)-; HASH(0x1283d0cc)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQWTQAYTPIGGNLLLSALAALVPIIFFFWALAIKRMKGYTAGLATLGIALIIAVLVYRM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
PAEKALMSATQGAVYGLLPIGWIIVTSVFLYKITVKTGQFDIIRSSVLSITDDRRLQALL
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHCCCCHHHHHHH
IAFSFGAFLEGAAGFGAPVAISAALLVGLGFNPLYAAGICLIANTAPVAFGAIGIPITAV
HHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCEEECCCCHHHCCCCCEEEEE
EGPTGIPAMEISQMVGRQLPFLSVFIPLYLIIIMSGFRKALEIWPAILVSGVSFAVVQYL
CCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSNFLGPELPDVLSALVSMAALAVFLKWWKPKTTFRFAGEQESAASIETARTNPAAPAYR
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHCCCCCCCCCCC
GGQIFKAWSPFLLLTAMISVWGIPSVKSALTGHYEGSAVFLKWLNAVGEKLTFSPGVPFL
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHCCCEECCCCCCCC
NNQIVNADGTPIEAVYKLEVLGSAGTAILIAAVLSKFITAISWKDWGTVFKETVQELKLP
CCEEECCCCCCHHHEEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCH
ILTIASVVGFAYVTNSSGMSTTLGMTLALTGSMFTFFSPVLGWLGVFITGSDTSANLLFG
HHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHCC
NLQKVTALSVGMDPVLSVAANSSGGVTGKMISPQSIAVACAAVGLAGKESDLFRFTIKHS
CHHHHHHHHCCCCHHHHHHCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHH
LFLLLLVCIITFLQHHVFSWMIP
HHHHHHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure
MQWTQAYTPIGGNLLLSALAALVPIIFFFWALAIKRMKGYTAGLATLGIALIIAVLVYRM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
PAEKALMSATQGAVYGLLPIGWIIVTSVFLYKITVKTGQFDIIRSSVLSITDDRRLQALL
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHCCCCHHHHHHH
IAFSFGAFLEGAAGFGAPVAISAALLVGLGFNPLYAAGICLIANTAPVAFGAIGIPITAV
HHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCEEECCCCHHHCCCCCEEEEE
EGPTGIPAMEISQMVGRQLPFLSVFIPLYLIIIMSGFRKALEIWPAILVSGVSFAVVQYL
CCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSNFLGPELPDVLSALVSMAALAVFLKWWKPKTTFRFAGEQESAASIETARTNPAAPAYR
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHCCCCCCCCCCC
GGQIFKAWSPFLLLTAMISVWGIPSVKSALTGHYEGSAVFLKWLNAVGEKLTFSPGVPFL
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHCCCEECCCCCCCC
NNQIVNADGTPIEAVYKLEVLGSAGTAILIAAVLSKFITAISWKDWGTVFKETVQELKLP
CCEEECCCCCCHHHEEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCH
ILTIASVVGFAYVTNSSGMSTTLGMTLALTGSMFTFFSPVLGWLGVFITGSDTSANLLFG
HHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHCC
NLQKVTALSVGMDPVLSVAANSSGGVTGKMISPQSIAVACAAVGLAGKESDLFRFTIKHS
CHHHHHHHHCCCCHHHHHHCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHH
LFLLLLVCIITFLQHHVFSWMIP
HHHHHHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969506