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The map label for this gene is araR

Identifier: 16080450

GI number: 16080450

Start: 3485604

End: 3486758

Strand: Direct

Name: araR

Synonym: BSU33970

Alternate gene names: 16080450

Gene position: 3485604-3486758 (Clockwise)

Preceding gene: 16080442

Following gene: 16080453

Centisome position: 82.68

GC content: 44.59

Gene sequence:

>1155_bases
ATGTTTTCTTACAAAGAACGCTGTGATATACTGAAATTTGTCCGTATACATTTTGGAGGAATGGATATGTTACCAAAATA
CGCGCAAGTAAAAGAAGAAATCAGTTCTTGGATTAATCAAGGCAAAATACTGCCCGATCAAAAAATCCCTACCGAAAACG
AATTAATGCAGCAATTCGGCGTCAGCCGGCATACCATCCGCAAAGCGATCGGAGACCTCGTATCACAAGGTCTGCTGTAC
AGCGTGCAAGGCGGAGGCACCTTTGTCGCTTCACGCTCTGCTAAGTCAGCGCTGCATTCCAATAAAACGATCGGTGTTTT
GACAACTTACATATCAGACTATATTTTCCCGAGCATCATCAGAGGAATCGAGTCCTATTTAAGCGAGCAGGGGTATTCTA
TGCTTTTGACAAGCACAAACAACAACCCGGACAATGAAAGAAGAGGCTTAGAAAACCTGCTGTCCCAGCATATTGACGGA
CTCATCGTAGAACCGACAAAAAGCGCCCTTCAAACCCCAAACATCGGCTATTATCTGAACTTGGAGAAAAACGGCATTCC
TTTTGCGATGATTAACGCGTCATATGCCGAGCTTGCCGCGCCAAGTTTTACCTTGGATGATGTGAAAGGCGGGATGATGG
CGGCGGAGCATTTGCTTTCTCTCGGCCACACGCATATGATGGGTATTTTTAAAGCTGATGACACACAAGGCGTGAAACGG
ATGAACGGATTTATACAGGCGCACCGGGAGCGTGAGTTGTTTCCTTCTCCGGATATGATCGTGACATTTACAACGGAAGA
AAAAGAATCAAAACTTCTGGAGAAAGTAAAAGCCACACTGGAGAAAAACAGCAAGCACATGCCGACAGCCATTCTTTGTT
ATAACGATGAAATTGCGCTGAAGGTGATTGATATGCTGAGGGAGATGGATCTTAAAGTGCCGGAGGATATGTCTATTGTC
GGGTACGATGATTCACATTTCGCCCAAATCTCAGAAGTGAAACTAACCTCTGTCAAACATCCGAAATCAGTGCTTGGAAA
AGCAGCCGCCAAATATGTCATTGACTGCTTAGAGCATAAAAAGCCGAAGCAAGAGGATGTCATATTTGAGCCTGAGTTGA
TCATTCGCCAGTCCGCACGAAAACTGAATGAATAA

Upstream 100 bases:

>100_bases
TTTAAACCTTCAATTGTACGTACTTATATTAAAATTTAACGAAAATTACATTTAATTAGTACGTACAAATATAGAATAAT
CTTGTTTGCATTTTCTGTCA

Downstream 100 bases:

>100_bases
AAAAAGCAATGTATGGGTCTCCCCGCTACATTGCTTTTTTTATAGCTGTTATCTGTTTTTCCAAACGTCAGCGATGATTT
CAAACGAACGCATTCTGTCT

Product: transcriptional repressor of the ara regulon (LacI family)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MFSYKERCDILKFVRIHFGGMDMLPKYAQVKEEISSWINQGKILPDQKIPTENELMQQFGVSRHTIRKAIGDLVSQGLLY
SVQGGGTFVASRSAKSALHSNKTIGVLTTYISDYIFPSIIRGIESYLSEQGYSMLLTSTNNNPDNERRGLENLLSQHIDG
LIVEPTKSALQTPNIGYYLNLEKNGIPFAMINASYAELAAPSFTLDDVKGGMMAAEHLLSLGHTHMMGIFKADDTQGVKR
MNGFIQAHRERELFPSPDMIVTFTTEEKESKLLEKVKATLEKNSKHMPTAILCYNDEIALKVIDMLREMDLKVPEDMSIV
GYDDSHFAQISEVKLTSVKHPKSVLGKAAAKYVIDCLEHKKPKQEDVIFEPELIIRQSARKLNE

Sequences:

>Translated_384_residues
MFSYKERCDILKFVRIHFGGMDMLPKYAQVKEEISSWINQGKILPDQKIPTENELMQQFGVSRHTIRKAIGDLVSQGLLY
SVQGGGTFVASRSAKSALHSNKTIGVLTTYISDYIFPSIIRGIESYLSEQGYSMLLTSTNNNPDNERRGLENLLSQHIDG
LIVEPTKSALQTPNIGYYLNLEKNGIPFAMINASYAELAAPSFTLDDVKGGMMAAEHLLSLGHTHMMGIFKADDTQGVKR
MNGFIQAHRERELFPSPDMIVTFTTEEKESKLLEKVKATLEKNSKHMPTAILCYNDEIALKVIDMLREMDLKVPEDMSIV
GYDDSHFAQISEVKLTSVKHPKSVLGKAAAKYVIDCLEHKKPKQEDVIFEPELIIRQSARKLNE
>Mature_384_residues
MFSYKERCDILKFVRIHFGGMDMLPKYAQVKEEISSWINQGKILPDQKIPTENELMQQFGVSRHTIRKAIGDLVSQGLLY
SVQGGGTFVASRSAKSALHSNKTIGVLTTYISDYIFPSIIRGIESYLSEQGYSMLLTSTNNNPDNERRGLENLLSQHIDG
LIVEPTKSALQTPNIGYYLNLEKNGIPFAMINASYAELAAPSFTLDDVKGGMMAAEHLLSLGHTHMMGIFKADDTQGVKR
MNGFIQAHRERELFPSPDMIVTFTTEEKESKLLEKVKATLEKNSKHMPTAILCYNDEIALKVIDMLREMDLKVPEDMSIV
GYDDSHFAQISEVKLTSVKHPKSVLGKAAAKYVIDCLEHKKPKQEDVIFEPELIIRQSARKLNE

Specific function: Transcriptional repressor of the arabinose utilization genes. Also regulates its own expression. Binds to two sequences within the promoters of the araABDLMNPQ-abfA operon and the araE gene, and to one sequence in the araR promoter [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=332, Percent_Identity=26.2048192771084, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787948, Length=299, Percent_Identity=27.0903010033445, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790194, Length=335, Percent_Identity=26.2686567164179, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI48994940, Length=274, Percent_Identity=24.8175182481752, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1788474, Length=341, Percent_Identity=23.7536656891496, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789202, Length=288, Percent_Identity=25, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1790715, Length=273, Percent_Identity=24.5421245421245, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87082252, Length=72, Percent_Identity=52.7777777777778, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1786950, Length=65, Percent_Identity=44.6153846153846, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789068, Length=349, Percent_Identity=22.0630372492837, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000524
- InterPro:   IPR001761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00392 GntR; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 43163; Mature: 43163

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSYKERCDILKFVRIHFGGMDMLPKYAQVKEEISSWINQGKILPDQKIPTENELMQQFG
CCCHHHHHHHHHHHHHHCCCHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHC
VSRHTIRKAIGDLVSQGLLYSVQGGGTFVASRSAKSALHSNKTIGVLTTYISDYIFPSII
CHHHHHHHHHHHHHHCCCEEEECCCCEEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RGIESYLSEQGYSMLLTSTNNNPDNERRGLENLLSQHIDGLIVEPTKSALQTPNIGYYLN
HHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEECCCHHHHCCCCCCEEEE
LEKNGIPFAMINASYAELAAPSFTLDDVKGGMMAAEHLLSLGHTHMMGIFKADDTQGVKR
EECCCCCEEEECCCHHHHCCCCCCHHHHCCHHHHHHHHHHHCCHHHEEEEECCCHHHHHH
MNGFIQAHRERELFPSPDMIVTFTTEEKESKLLEKVKATLEKNSKHMPTAILCYNDEIAL
HHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHH
KVIDMLREMDLKVPEDMSIVGYDDSHFAQISEVKLTSVKHPKSVLGKAAAKYVIDCLEHK
HHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
KPKQEDVIFEPELIIRQSARKLNE
CCCCCCCEECCHHHHHHHHHHCCC
>Mature Secondary Structure
MFSYKERCDILKFVRIHFGGMDMLPKYAQVKEEISSWINQGKILPDQKIPTENELMQQFG
CCCHHHHHHHHHHHHHHCCCHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHC
VSRHTIRKAIGDLVSQGLLYSVQGGGTFVASRSAKSALHSNKTIGVLTTYISDYIFPSII
CHHHHHHHHHHHHHHCCCEEEECCCCEEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RGIESYLSEQGYSMLLTSTNNNPDNERRGLENLLSQHIDGLIVEPTKSALQTPNIGYYLN
HHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEECCCHHHHCCCCCCEEEE
LEKNGIPFAMINASYAELAAPSFTLDDVKGGMMAAEHLLSLGHTHMMGIFKADDTQGVKR
EECCCCCEEEECCCHHHHCCCCCCHHHHCCHHHHHHHHHHHCCHHHEEEEECCCHHHHHH
MNGFIQAHRERELFPSPDMIVTFTTEEKESKLLEKVKATLEKNSKHMPTAILCYNDEIAL
HHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHH
KVIDMLREMDLKVPEDMSIVGYDDSHFAQISEVKLTSVKHPKSVLGKAAAKYVIDCLEHK
HHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
KPKQEDVIFEPELIIRQSARKLNE
CCCCCCCEECCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9045819; 9384377; 10417639; 11418559 [H]