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The map label for this gene is groEL

Identifier: 15607130

GI number: 15607130

Start: 1547532

End: 1549169

Strand: Direct

Name: groEL

Synonym: aq_2200

Alternate gene names: 15607130

Gene position: 1547532-1549169 (Clockwise)

Preceding gene: 15607129

Following gene: NA

Centisome position: 99.75

GC content: 45.85

Gene sequence:

>1638_bases
ATGGCAGCAAAGGCAATTATCTACAACGAGGAAGCAAGGGCAAAACTAAAGGCTGGTGTTGATAAACTCGCAAACGCAGT
TAAGGTTACCTTAGGTCCAAAAGGTAGGGAAGTTATCTTAGGAAAGAACTGGGGAACTCCCGTTGTAACCAAGGACGGTG
TTACGGTAGCTAAGGAAATTGAACTCAAGGACAAGTTTGAAAATATCGGAGCTCAACTCGTAAAAGAAGTTGCTTCCAAG
ACCGCGGACGTAGCAGGTGACGGAACTACCACCGCAACGGTACTCGCACAGGCAATATTCCACGAAGGACTCAGGGTTGC
AGCAAGCGGTGCAAACGTAATGGAAGTAAAGAGGGGAATTGACAAGGCCGTAAAGAAAATTGTTGAAGAACTCAAGAAAC
TCTCCAAGGACGTAAAGGAAAGGAAGGAAATAGAACAGGTAGCTACCATATCCGCTAACAACGATCCCGAAATCGGAAAG
ATAATAGCGGACGCTATGGAAGAAGTTGGAAAGGACGGGGTTATAACTGTTGAAGAAAGCAAGTCCGCGGAAACCACCCT
CGAAGTAGTTAAGGGAATGCAGTTTGACAGAGGATACCTATCTCCGTACTTTGTAACAGATCCTGAGAAGATGGAATGCG
TACTAGAAAATCCCTACATACTCATATACGAAAAGAAGATAACCAACGTAAAGGAACTTCTTCCAATACTTGAACAAGTA
GTAAGAAGCGGAAGACCACTTCTGGTTATAGCTGAAGACGTAGAAGGTGAAGCACTCGCAACACTCGTTGTAAACCACAT
CAAAGGAGTACTCAAGGCTTGTGCAGTAAAGGCTCCCGGATTCGGACAAAGGAGAAAGGACTACCTCGGAGATATTGCAG
TTCTCACAGGCGGACAGGCTATAACTGAAGACCTCGGAATTAAGCTTGAAAGCGTAACACTTGACATGCTCGGACAGGCT
GAAAAGGTAGTGGTTGATAAAGAACACACCACCATAATAGGCGGTAAGGGAGATCCCGAACAAATAAAGGCAAGGATAGA
ACAAATAAAGAGACAAATTCAGGAAACAACCTCTGACTACGACAGGGAAAAGCTACAAGAAAGACTCGCTAAACTCTCAG
GTGGAGTTGCAATAATAAGGGTAGGTGCTGCAACCGAAGCTGAACTCAAAGAGAAGAAGTACAGAGTTGAAGACGCGGTA
CACGCAACCAAGGCTGCAGTAGAAGAAGGAATAGTTCCCGGTGGTGGAGTAGCACTTGTGAGGGCATCCGAAGCACTTGA
AGACCTCAAAGGTGACAACCACGATCAACAACTCGGAATAGACATAATCAAGAAGGCTGTAAGGACACCCCTCAAGCAAA
TAGCTTACAACGCAGGATACGACGGATCCGTAGTACTCGAAAAGGTGATTGAACTCGGAAAGGAAAAAGGTGTAAGCTGG
GGATTCAACGCGGCAACCGGAGAGTACGTTGACATGTACGAAGCCGGAATAATAGATCCGACCAAAGTCGTAAGAACCGC
TATAGAAAACGCGGCATCCGTAGCAGGAACGATGCTCACCGCTGAAGCACTGATAGCAGACCTTCCCGAAGAAAAGAAGA
AAGACATAACTCCCACTGACATGCCTGAACTTGACTAA

Upstream 100 bases:

>100_bases
ACGAAGTAGAGATTGAAGGAAAGATTTACCTCGTTATGTCTGAAGACGAAGTTTTAGCTGTTGTTGAAGATTATTCAAGC
TTAATAGGAGGTGAGGTGAG

Downstream 100 bases:

>100_bases
TTCCAGCCCCCTCTGGGGGTTTTTTATCTATTTAAATGAAAAGATAAAAATTTCTCCAAAATCTCCCTTCCCTCTTCAAA
ATCTTTTCTTTTTATTTTTA

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MAAKAIIYNEEARAKLKAGVDKLANAVKVTLGPKGREVILGKNWGTPVVTKDGVTVAKEIELKDKFENIGAQLVKEVASK
TADVAGDGTTTATVLAQAIFHEGLRVAASGANVMEVKRGIDKAVKKIVEELKKLSKDVKERKEIEQVATISANNDPEIGK
IIADAMEEVGKDGVITVEESKSAETTLEVVKGMQFDRGYLSPYFVTDPEKMECVLENPYILIYEKKITNVKELLPILEQV
VRSGRPLLVIAEDVEGEALATLVVNHIKGVLKACAVKAPGFGQRRKDYLGDIAVLTGGQAITEDLGIKLESVTLDMLGQA
EKVVVDKEHTTIIGGKGDPEQIKARIEQIKRQIQETTSDYDREKLQERLAKLSGGVAIIRVGAATEAELKEKKYRVEDAV
HATKAAVEEGIVPGGGVALVRASEALEDLKGDNHDQQLGIDIIKKAVRTPLKQIAYNAGYDGSVVLEKVIELGKEKGVSW
GFNAATGEYVDMYEAGIIDPTKVVRTAIENAASVAGTMLTAEALIADLPEEKKKDITPTDMPELD

Sequences:

>Translated_545_residues
MAAKAIIYNEEARAKLKAGVDKLANAVKVTLGPKGREVILGKNWGTPVVTKDGVTVAKEIELKDKFENIGAQLVKEVASK
TADVAGDGTTTATVLAQAIFHEGLRVAASGANVMEVKRGIDKAVKKIVEELKKLSKDVKERKEIEQVATISANNDPEIGK
IIADAMEEVGKDGVITVEESKSAETTLEVVKGMQFDRGYLSPYFVTDPEKMECVLENPYILIYEKKITNVKELLPILEQV
VRSGRPLLVIAEDVEGEALATLVVNHIKGVLKACAVKAPGFGQRRKDYLGDIAVLTGGQAITEDLGIKLESVTLDMLGQA
EKVVVDKEHTTIIGGKGDPEQIKARIEQIKRQIQETTSDYDREKLQERLAKLSGGVAIIRVGAATEAELKEKKYRVEDAV
HATKAAVEEGIVPGGGVALVRASEALEDLKGDNHDQQLGIDIIKKAVRTPLKQIAYNAGYDGSVVLEKVIELGKEKGVSW
GFNAATGEYVDMYEAGIIDPTKVVRTAIENAASVAGTMLTAEALIADLPEEKKKDITPTDMPELD
>Mature_544_residues
AAKAIIYNEEARAKLKAGVDKLANAVKVTLGPKGREVILGKNWGTPVVTKDGVTVAKEIELKDKFENIGAQLVKEVASKT
ADVAGDGTTTATVLAQAIFHEGLRVAASGANVMEVKRGIDKAVKKIVEELKKLSKDVKERKEIEQVATISANNDPEIGKI
IADAMEEVGKDGVITVEESKSAETTLEVVKGMQFDRGYLSPYFVTDPEKMECVLENPYILIYEKKITNVKELLPILEQVV
RSGRPLLVIAEDVEGEALATLVVNHIKGVLKACAVKAPGFGQRRKDYLGDIAVLTGGQAITEDLGIKLESVTLDMLGQAE
KVVVDKEHTTIIGGKGDPEQIKARIEQIKRQIQETTSDYDREKLQERLAKLSGGVAIIRVGAATEAELKEKKYRVEDAVH
ATKAAVEEGIVPGGGVALVRASEALEDLKGDNHDQQLGIDIIKKAVRTPLKQIAYNAGYDGSVVLEKVIELGKEKGVSWG
FNAATGEYVDMYEAGIIDPTKVVRTAIENAASVAGTMLTAEALIADLPEEKKKDITPTDMPELD

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=532, Percent_Identity=51.5037593984962, Blast_Score=525, Evalue=1e-149,
Organism=Homo sapiens, GI31542947, Length=532, Percent_Identity=51.5037593984962, Blast_Score=525, Evalue=1e-149,
Organism=Homo sapiens, GI5453607, Length=519, Percent_Identity=23.121387283237, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1790586, Length=536, Percent_Identity=59.8880597014925, Blast_Score=640, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=530, Percent_Identity=49.622641509434, Blast_Score=521, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI193210679, Length=241, Percent_Identity=46.0580912863071, Blast_Score=217, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=54.1825095057034, Blast_Score=551, Evalue=1e-158,
Organism=Saccharomyces cerevisiae, GI6322524, Length=176, Percent_Identity=27.2727272727273, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24641193, Length=530, Percent_Identity=51.5094339622642, Blast_Score=528, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24641191, Length=530, Percent_Identity=51.5094339622642, Blast_Score=528, Evalue=1e-150,
Organism=Drosophila melanogaster, GI45550936, Length=530, Percent_Identity=50.7547169811321, Blast_Score=519, Evalue=1e-147,
Organism=Drosophila melanogaster, GI45550132, Length=530, Percent_Identity=50.7547169811321, Blast_Score=519, Evalue=1e-147,
Organism=Drosophila melanogaster, GI45550935, Length=530, Percent_Identity=50.7547169811321, Blast_Score=519, Evalue=1e-147,
Organism=Drosophila melanogaster, GI17864606, Length=529, Percent_Identity=45.179584120983, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24584129, Length=534, Percent_Identity=37.6404494382022, Blast_Score=329, Evalue=2e-90,
Organism=Drosophila melanogaster, GI19921262, Length=534, Percent_Identity=37.6404494382022, Blast_Score=329, Evalue=2e-90,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH60_AQUAE (O67943)

Other databases:

- EMBL:   AE000657
- PIR:   C70489
- RefSeq:   NP_214512.1
- ProteinModelPortal:   O67943
- GeneID:   1193993
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_2200
- NMPDR:   fig|224324.1.peg.1527
- HOGENOM:   HBG625289
- OMA:   EDPAENT
- PhylomeDB:   O67943
- ProtClustDB:   PRK00013
- BioCyc:   AAEO224324:AQ_2200-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 58795; Mature: 58664

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKAIIYNEEARAKLKAGVDKLANAVKVTLGPKGREVILGKNWGTPVVTKDGVTVAKEI
CCCCEEEECCHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCEECCCCEEHHHH
ELKDKFENIGAQLVKEVASKTADVAGDGTTTATVLAQAIFHEGLRVAASGANVMEVKRGI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCEEECCCCHHHHHHHHH
DKAVKKIVEELKKLSKDVKERKEIEQVATISANNDPEIGKIIADAMEEVGKDGVITVEES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECC
KSAETTLEVVKGMQFDRGYLSPYFVTDPEKMECVLENPYILIYEKKITNVKELLPILEQV
CCHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHEECCCEEEEEEHHHCCHHHHHHHHHHH
VRSGRPLLVIAEDVEGEALATLVVNHIKGVLKACAVKAPGFGQRRKDYLGDIAVLTGGQA
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCEEEEECCHH
ITEDLGIKLESVTLDMLGQAEKVVVDKEHTTIIGGKGDPEQIKARIEQIKRQIQETTSDY
HHHHHCCEEEEEHHHHHCCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
DREKLQERLAKLSGGVAIIRVGAATEAELKEKKYRVEDAVHATKAAVEEGIVPGGGVALV
HHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
RASEALEDLKGDNHDQQLGIDIIKKAVRTPLKQIAYNAGYDGSVVLEKVIELGKEKGVSW
EHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
GFNAATGEYVDMYEAGIIDPTKVVRTAIENAASVAGTMLTAEALIADLPEEKKKDITPTD
CCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCC
MPELD
CCCCC
>Mature Secondary Structure 
AAKAIIYNEEARAKLKAGVDKLANAVKVTLGPKGREVILGKNWGTPVVTKDGVTVAKEI
CCCEEEECCHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCEECCCCEEHHHH
ELKDKFENIGAQLVKEVASKTADVAGDGTTTATVLAQAIFHEGLRVAASGANVMEVKRGI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCEEECCCCHHHHHHHHH
DKAVKKIVEELKKLSKDVKERKEIEQVATISANNDPEIGKIIADAMEEVGKDGVITVEES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECC
KSAETTLEVVKGMQFDRGYLSPYFVTDPEKMECVLENPYILIYEKKITNVKELLPILEQV
CCHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHEECCCEEEEEEHHHCCHHHHHHHHHHH
VRSGRPLLVIAEDVEGEALATLVVNHIKGVLKACAVKAPGFGQRRKDYLGDIAVLTGGQA
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCEEEEECCHH
ITEDLGIKLESVTLDMLGQAEKVVVDKEHTTIIGGKGDPEQIKARIEQIKRQIQETTSDY
HHHHHCCEEEEEHHHHHCCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
DREKLQERLAKLSGGVAIIRVGAATEAELKEKKYRVEDAVHATKAAVEEGIVPGGGVALV
HHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
RASEALEDLKGDNHDQQLGIDIIKKAVRTPLKQIAYNAGYDGSVVLEKVIELGKEKGVSW
EHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
GFNAATGEYVDMYEAGIIDPTKVVRTAIENAASVAGTMLTAEALIADLPEEKKKDITPTD
CCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCC
MPELD
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320