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The map label for this gene is nifS

Identifier: 16520012

GI number: 16520012

Start: 114799

End: 115962

Strand: Direct

Name: nifS

Synonym: NGR_a00930

Alternate gene names: 16520012

Gene position: 114799-115962 (Clockwise)

Preceding gene: 16520013

Following gene: 16520011

Centisome position: 21.41

GC content: 56.62

Gene sequence:

>1164_bases
TTGAGGCCTATCTATCTCGACAACAACGCAACGACGCGCGTCGATGCGGAAGTTCTCCAGGCGATGCTGCCATTCTTTGC
AGATGTGTTTGGCAACGCTTCCTCGATGCATGACGCTGGTGCTACTGCCGGGGCAGCGATTAACGTAGCGCGCAGGCAGT
TGCAAGCACTGATCGGTGCAAAGTTCGACCCAGAGATCACCTTTACCTCGGGCGGGACCGAAAGCAACAATGCGGCGATC
CTTTCGGGGCTCGAGGCTATGCCTGAGCGCACTGAGATCGTAACTTCGGCGGTCGAGCATCCTGCGGTGCTGACGCTCTG
CACCCATCTGGAGAAGACACGCGGCACCAAGGTGCACAAGGTCCCAGTGGATCACCAGGGCCGACTTGACCTTGACGCTT
ATCAGGACGCCCTTACACACCGTGTAGCAATCGTTTCGATCATGTGGGCCAACAACGAGACGGGAACGATCTTTCCTGTG
GTAAAGCTAGCTGAGATGGCCAAAAAAGTCGGCGCAATTTTCCACACCGATGCGGTGCAAGCAATCGGCAAGCTACCGAT
TGACTTGAAATCAACGGCGATCGACATGCTGTCGCTTTCCGCGCACAAGTTCCATGGCCCGAAGGGCGTCGGGGCACTCT
ACATAAAGCGCGGGGTACCCTTTCACGCACTCATCAAGGGTGGCCACCAGGAGCGCGACCGGCGCGCGGGCACGGAGAAC
ACGCCCGGGATAGTAGGTCTGGGCAAGGCGGCAGAACTCGCGTTGGATTGTATGGACAAGGACAATGCGATAATAAGATC
ATTCCGCGATCGATTGGAGAAAGGACTTCTCGAGCGTGTCCCCCAAGTCTTCGTAATGGGTGATCCGGTGACGCGGTTGC
CCAACACAACGAGCATTGCCTTTGAAGGCGTCGGTGGTGAAGCCATGCAGTTTCTGCTCAATCGCCATGGCATCGCCTGC
TCCTCCGGATCCGCCTGTACCTCTCGTTCTTTATCGACGAGCCATGTGCTAAAAGCCATGGGTACTCCCCACAGGCAGGC
GGTCGGAGCAGTCCGCTTCTCGTTGTCAGGCTATAACTGTGAAGAGGATATAGATCGGGTGTTGCGGGTGATACCCGCAG
TTGTGAAAAAACTGCGTGAATCTCGTCCAGTATTTGCCGGGTAG

Upstream 100 bases:

>100_bases
CACGCACGGGAGAACAGTAATGCTGGATCAAAAAGCACTTCTACAATGCCACGATCGCCATTTGTAAACCTTTCGTCGTT
TCGGTAGCTGGAGAATTATC

Downstream 100 bases:

>100_bases
ACGCTCTGCCGGCACATACGATTGTTTGAGCGCTTGGGCATGTCCGAATAGCCGTCACTGATGGCGTCTCGATCACAGCT
CTTCCCAGGGTCATCATAGG

Product: nitrogen fixation protein NifS

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein NifS

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MRPIYLDNNATTRVDAEVLQAMLPFFADVFGNASSMHDAGATAGAAINVARRQLQALIGAKFDPEITFTSGGTESNNAAI
LSGLEAMPERTEIVTSAVEHPAVLTLCTHLEKTRGTKVHKVPVDHQGRLDLDAYQDALTHRVAIVSIMWANNETGTIFPV
VKLAEMAKKVGAIFHTDAVQAIGKLPIDLKSTAIDMLSLSAHKFHGPKGVGALYIKRGVPFHALIKGGHQERDRRAGTEN
TPGIVGLGKAAELALDCMDKDNAIIRSFRDRLEKGLLERVPQVFVMGDPVTRLPNTTSIAFEGVGGEAMQFLLNRHGIAC
SSGSACTSRSLSTSHVLKAMGTPHRQAVGAVRFSLSGYNCEEDIDRVLRVIPAVVKKLRESRPVFAG

Sequences:

>Translated_387_residues
MRPIYLDNNATTRVDAEVLQAMLPFFADVFGNASSMHDAGATAGAAINVARRQLQALIGAKFDPEITFTSGGTESNNAAI
LSGLEAMPERTEIVTSAVEHPAVLTLCTHLEKTRGTKVHKVPVDHQGRLDLDAYQDALTHRVAIVSIMWANNETGTIFPV
VKLAEMAKKVGAIFHTDAVQAIGKLPIDLKSTAIDMLSLSAHKFHGPKGVGALYIKRGVPFHALIKGGHQERDRRAGTEN
TPGIVGLGKAAELALDCMDKDNAIIRSFRDRLEKGLLERVPQVFVMGDPVTRLPNTTSIAFEGVGGEAMQFLLNRHGIAC
SSGSACTSRSLSTSHVLKAMGTPHRQAVGAVRFSLSGYNCEEDIDRVLRVIPAVVKKLRESRPVFAG
>Mature_387_residues
MRPIYLDNNATTRVDAEVLQAMLPFFADVFGNASSMHDAGATAGAAINVARRQLQALIGAKFDPEITFTSGGTESNNAAI
LSGLEAMPERTEIVTSAVEHPAVLTLCTHLEKTRGTKVHKVPVDHQGRLDLDAYQDALTHRVAIVSIMWANNETGTIFPV
VKLAEMAKKVGAIFHTDAVQAIGKLPIDLKSTAIDMLSLSAHKFHGPKGVGALYIKRGVPFHALIKGGHQERDRRAGTEN
TPGIVGLGKAAELALDCMDKDNAIIRSFRDRLEKGLLERVPQVFVMGDPVTRLPNTTSIAFEGVGGEAMQFLLNRHGIAC
SSGSACTSRSLSTSHVLKAMGTPHRQAVGAVRFSLSGYNCEEDIDRVLRVIPAVVKKLRESRPVFAG

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily

Homologues:

Organism=Homo sapiens, GI32307132, Length=390, Percent_Identity=41.025641025641, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI156713448, Length=417, Percent_Identity=35.9712230215827, Blast_Score=199, Evalue=4e-51,
Organism=Escherichia coli, GI48994898, Length=390, Percent_Identity=39.7435897435897, Blast_Score=258, Evalue=4e-70,
Organism=Escherichia coli, GI1789175, Length=391, Percent_Identity=27.3657289002558, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787970, Length=387, Percent_Identity=26.6149870801034, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI25143064, Length=390, Percent_Identity=38.974358974359, Blast_Score=261, Evalue=4e-70,
Organism=Caenorhabditis elegans, GI17533177, Length=301, Percent_Identity=33.5548172757475, Blast_Score=154, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6319831, Length=390, Percent_Identity=40.7692307692308, Blast_Score=260, Evalue=2e-70,
Organism=Drosophila melanogaster, GI20129463, Length=390, Percent_Identity=40.2564102564103, Blast_Score=260, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIFS_RHISN (P55690)

Other databases:

- EMBL:   U00090
- RefSeq:   NP_444132.1
- ProteinModelPortal:   P55690
- SMR:   P55690
- GeneID:   962357
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a00930
- HOGENOM:   HBG635316
- ProtClustDB:   CLSK506492
- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PIRSF:   PIRSF005572
- TIGRFAMs:   TIGR03402

Pfam domain/function: PF00266 Aminotran_5; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.8.1.7

Molecular weight: Translated: 41617; Mature: 41617

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: ACT_SITE 326-326

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPIYLDNNATTRVDAEVLQAMLPFFADVFGNASSMHDAGATAGAAINVARRQLQALIGA
CCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
KFDPEITFTSGGTESNNAAILSGLEAMPERTEIVTSAVEHPAVLTLCTHLEKTRGTKVHK
CCCCCEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEE
VPVDHQGRLDLDAYQDALTHRVAIVSIMWANNETGTIFPVVKLAEMAKKVGAIFHTDAVQ
CCCCCCCCCCHHHHHHHHHHHEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AIGKLPIDLKSTAIDMLSLSAHKFHGPKGVGALYIKRGVPFHALIKGGHQERDRRAGTEN
HHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHCCCCHHHHHHCCCCC
TPGIVGLGKAAELALDCMDKDNAIIRSFRDRLEKGLLERVPQVFVMGDPVTRLPNTTSIA
CCCEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHCCCCCEEE
FEGVGGEAMQFLLNRHGIACSSGSACTSRSLSTSHVLKAMGTPHRQAVGAVRFSLSGYNC
EECCCHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHCCCHHHHHHHHEEEECCCCC
EEDIDRVLRVIPAVVKKLRESRPVFAG
HHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRPIYLDNNATTRVDAEVLQAMLPFFADVFGNASSMHDAGATAGAAINVARRQLQALIGA
CCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
KFDPEITFTSGGTESNNAAILSGLEAMPERTEIVTSAVEHPAVLTLCTHLEKTRGTKVHK
CCCCCEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEE
VPVDHQGRLDLDAYQDALTHRVAIVSIMWANNETGTIFPVVKLAEMAKKVGAIFHTDAVQ
CCCCCCCCCCHHHHHHHHHHHEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AIGKLPIDLKSTAIDMLSLSAHKFHGPKGVGALYIKRGVPFHALIKGGHQERDRRAGTEN
HHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHCCCCHHHHHHCCCCC
TPGIVGLGKAAELALDCMDKDNAIIRSFRDRLEKGLLERVPQVFVMGDPVTRLPNTTSIA
CCCEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHCCCCCEEE
FEGVGGEAMQFLLNRHGIACSSGSACTSRSLSTSHVLKAMGTPHRQAVGAVRFSLSGYNC
EECCCHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHCCCHHHHHHHHEEEECCCCC
EEDIDRVLRVIPAVVKKLRESRPVFAG
HHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424