BacMap

Definition	Sinorhizobium fredii NGR234 plasmid pNGR234a, complete sequence.
Accession	NC_000914
Length	536,165

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The map label for this gene is Not Available

Identifier: 16519862

GI number: 16519862

Start: 321336

End: 322097

Strand: Direct

Name: Not Available

Synonym: NGR_a02430

Alternate gene names: 16519862

Gene position: 321336-322097 (Clockwise)

Preceding gene: 16519864

Following gene: 16519861

Centisome position: 59.93

GC content: 62.86

Gene sequence:

>762_bases
ATGCTTTTGAAGGGAAAGACCGCCGTCATCTCTGGCGCGGCGAGCAAGCGTGGCATTGGCCGGGCAACGGCGGAGCTTTT
TGCATCGCACGGCGCGCGGGTCGCCATTCTTGACATCAACGCCGATGAGGCAAAGGCTGCAGCGGGCGACCTGCCGCCGG
TCGAACACGGTGCGCATATCGGGCTGCGTTGCGACGTTGCCGATCGTGCCTCCTGCACGTCCGCCTCGGATGAGGTCTTG
AGCGCGTTTGGCGTCGCCAACATCCTCATCAACAATGCCGGCATCACCCAGCCGGTGAAGACCCTCGATATCTCTGACGC
CGACTGGCAGCGCATCGTCGCCGTCAACATGACGGGCGTGCTCAATCTCAGTCAGGTTTTCATACCAAACATGCGGCAGA
ATGGCGGTGGATCGATCGCCTGCATGTCGTCGGTTTCAGCGCAGCGCGGCGGCGGAATCTTCGGCGGCCCCCATTATTCG
GCGGCCAAGGCCGGCGTGCTCGGCCTTGCCAAGGCCATGGCCAGGGAGTTCGGCCCCGACAGCATTCGCGTCAATTGCGT
GACACCGGGGCTCATTCAGACCGACATCACCGGCGACAAGCTGTCCGCCGAGATGCGCGCAGACATCGTCAAGGGCATTC
CGCTCAGTCGTTTGGGCGACGCGCGCGACGTGGCCAACATCTATCTCTTCCTCGCATCCGATCTCTCCGCCTACGTCACG
GGCGCCGTCATCGACGTCAATGGCGGCATGCTGATCCATTGA

Upstream 100 bases:

>100_bases
AGGACGGTGCGCGCTACGCCACAACTCTTCCACGAGGACAGGCAGAGGCCTGGAAGGAGACACCAGGCGCCGCATAGTCG
GTGTCGGGAGGAAAGAAAAG

Downstream 100 bases:

>100_bases
TCCACGAGGAAGCCTGAAAGGAAGGACGGCATGGCTCAGATCGGTCACAACATCAGTCTGCCCGAGCGTGCGCGCCGCAT
TCGCCGGCATGCGCTCCGCA

Product: short chain dehydrogenase/reductase

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MLLKGKTAVISGAASKRGIGRATAELFASHGARVAILDINADEAKAAAGDLPPVEHGAHIGLRCDVADRASCTSASDEVL
SAFGVANILINNAGITQPVKTLDISDADWQRIVAVNMTGVLNLSQVFIPNMRQNGGGSIACMSSVSAQRGGGIFGGPHYS
AAKAGVLGLAKAMAREFGPDSIRVNCVTPGLIQTDITGDKLSAEMRADIVKGIPLSRLGDARDVANIYLFLASDLSAYVT
GAVIDVNGGMLIH

Sequences:

>Translated_253_residues
MLLKGKTAVISGAASKRGIGRATAELFASHGARVAILDINADEAKAAAGDLPPVEHGAHIGLRCDVADRASCTSASDEVL
SAFGVANILINNAGITQPVKTLDISDADWQRIVAVNMTGVLNLSQVFIPNMRQNGGGSIACMSSVSAQRGGGIFGGPHYS
AAKAGVLGLAKAMAREFGPDSIRVNCVTPGLIQTDITGDKLSAEMRADIVKGIPLSRLGDARDVANIYLFLASDLSAYVT
GAVIDVNGGMLIH
>Mature_253_residues
MLLKGKTAVISGAASKRGIGRATAELFASHGARVAILDINADEAKAAAGDLPPVEHGAHIGLRCDVADRASCTSASDEVL
SAFGVANILINNAGITQPVKTLDISDADWQRIVAVNMTGVLNLSQVFIPNMRQNGGGSIACMSSVSAQRGGGIFGGPHYS
AAKAGVLGLAKAMAREFGPDSIRVNCVTPGLIQTDITGDKLSAEMRADIVKGIPLSRLGDARDVANIYLFLASDLSAYVT
GAVIDVNGGMLIH

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family

Homologues:

Organism=Homo sapiens, GI40254992, Length=234, Percent_Identity=36.3247863247863, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI15277342, Length=260, Percent_Identity=35, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI19923817, Length=260, Percent_Identity=28.0769230769231, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI66933014, Length=229, Percent_Identity=33.1877729257642, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI10190704, Length=256, Percent_Identity=31.25, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI32483357, Length=250, Percent_Identity=29.6, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI7705925, Length=252, Percent_Identity=28.5714285714286, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI59889578, Length=230, Percent_Identity=31.304347826087, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI126723750, Length=254, Percent_Identity=29.1338582677165, Blast_Score=91, Evalue=7e-19,
Organism=Homo sapiens, GI5031737, Length=249, Percent_Identity=31.3253012048193, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI31542939, Length=209, Percent_Identity=32.0574162679426, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI4503301, Length=230, Percent_Identity=29.5652173913043, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI4758504, Length=267, Percent_Identity=29.5880149812734, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI4504505, Length=258, Percent_Identity=29.8449612403101, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI224922801, Length=185, Percent_Identity=32.4324324324324, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI33667109, Length=197, Percent_Identity=31.4720812182741, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI310128830, Length=199, Percent_Identity=27.6381909547739, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI310115468, Length=199, Percent_Identity=27.6381909547739, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI20149619, Length=208, Percent_Identity=25.9615384615385, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI210032110, Length=201, Percent_Identity=24.8756218905473, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI40807363, Length=196, Percent_Identity=30.1020408163265, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI83715985, Length=267, Percent_Identity=28.0898876404494, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1787335, Length=253, Percent_Identity=40.3162055335968, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1787905, Length=247, Percent_Identity=35.2226720647773, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI87082100, Length=262, Percent_Identity=34.3511450381679, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI87082160, Length=257, Percent_Identity=33.4630350194552, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1789208, Length=251, Percent_Identity=31.8725099601594, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790717, Length=251, Percent_Identity=29.0836653386454, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1786812, Length=258, Percent_Identity=31.7829457364341, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788459, Length=249, Percent_Identity=30.5220883534137, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI2367175, Length=257, Percent_Identity=33.0739299610895, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1789378, Length=254, Percent_Identity=31.496062992126, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789057, Length=240, Percent_Identity=29.5833333333333, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI2367365, Length=247, Percent_Identity=31.1740890688259, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787526, Length=254, Percent_Identity=26.7716535433071, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787545, Length=269, Percent_Identity=29.368029739777, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788891, Length=247, Percent_Identity=27.1255060728745, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25147288, Length=251, Percent_Identity=40.6374501992032, Blast_Score=170, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17555706, Length=250, Percent_Identity=36.4, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17561402, Length=255, Percent_Identity=31.7647058823529, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17560676, Length=256, Percent_Identity=30.859375, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17536651, Length=254, Percent_Identity=29.9212598425197, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17544670, Length=275, Percent_Identity=30.1818181818182, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17536025, Length=252, Percent_Identity=28.5714285714286, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17561272, Length=242, Percent_Identity=30.5785123966942, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17565030, Length=264, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17567345, Length=203, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71994600, Length=259, Percent_Identity=29.7297297297297, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17560150, Length=272, Percent_Identity=27.9411764705882, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17562990, Length=268, Percent_Identity=28.7313432835821, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17562906, Length=270, Percent_Identity=30, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17559104, Length=263, Percent_Identity=29.6577946768061, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17551412, Length=262, Percent_Identity=29.0076335877863, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17562910, Length=267, Percent_Identity=29.9625468164794, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI72000259, Length=262, Percent_Identity=27.4809160305344, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71994604, Length=252, Percent_Identity=28.1746031746032, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI115534694, Length=252, Percent_Identity=30.1587301587302, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17538480, Length=268, Percent_Identity=29.1044776119403, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17562904, Length=273, Percent_Identity=27.1062271062271, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17560332, Length=265, Percent_Identity=28.3018867924528, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17538182, Length=262, Percent_Identity=27.4809160305344, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI193203115, Length=196, Percent_Identity=29.5918367346939, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI193203117, Length=194, Percent_Identity=29.8969072164948, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17563726, Length=259, Percent_Identity=30.5019305019305, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17564282, Length=196, Percent_Identity=29.5918367346939, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17531453, Length=267, Percent_Identity=28.4644194756554, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17568967, Length=209, Percent_Identity=28.7081339712919, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI193204405, Length=268, Percent_Identity=28.3582089552239, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17508895, Length=197, Percent_Identity=25.3807106598985, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17538486, Length=262, Percent_Identity=28.6259541984733, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=268, Percent_Identity=27.2388059701493, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17560220, Length=264, Percent_Identity=26.5151515151515, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=253, Percent_Identity=28.8537549407115, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6322861, Length=197, Percent_Identity=30.9644670050761, Blast_Score=83, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24639444, Length=252, Percent_Identity=34.9206349206349, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI21355319, Length=250, Percent_Identity=33.2, Blast_Score=124, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24644339, Length=254, Percent_Identity=30.7086614173228, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI23397609, Length=254, Percent_Identity=29.5275590551181, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24643142, Length=251, Percent_Identity=31.0756972111554, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI28571526, Length=252, Percent_Identity=28.968253968254, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI21357041, Length=258, Percent_Identity=30.2325581395349, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17737361, Length=264, Percent_Identity=28.7878787878788, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24641232, Length=224, Percent_Identity=27.6785714285714, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI18859837, Length=220, Percent_Identity=31.8181818181818, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281364892, Length=194, Percent_Identity=31.4432989690722, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21358495, Length=213, Percent_Identity=30.0469483568075, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24649181, Length=197, Percent_Identity=25.8883248730964, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI221331216, Length=204, Percent_Identity=31.3725490196078, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24665247, Length=204, Percent_Identity=31.3725490196078, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI221331218, Length=203, Percent_Identity=31.0344827586207, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24665243, Length=203, Percent_Identity=31.0344827586207, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI116007234, Length=202, Percent_Identity=30.6930693069307, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24581726, Length=177, Percent_Identity=27.683615819209, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI116007236, Length=234, Percent_Identity=26.9230769230769, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24644337, Length=175, Percent_Identity=32, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI221510726, Length=187, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI281364494, Length=206, Percent_Identity=26.2135922330097, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y4MP_RHISN (P55575)

Other databases:

- EMBL:   U00090
- RefSeq:   NP_443982.1
- ProteinModelPortal:   P55575
- SMR:   P55575
- GeneID:   962196
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a02430
- HOGENOM:   HBG750976
- ProtClustDB:   CLSK864386
- GO:   GO:0005488
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR19410
- PRINTS:   PR00081
- PRINTS:   PR00080

Pfam domain/function: PF00106 adh_short

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 25995; Mature: 25995

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00061 ADH_SHORT

Important sites: ACT_SITE 159-159 BINDING 144-144

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKGKTAVISGAASKRGIGRATAELFASHGARVAILDINADEAKAAAGDLPPVEHGAHI
CCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHCCCCCCCCCCCCEE
GLRCDVADRASCTSASDEVLSAFGVANILINNAGITQPVKTLDISDADWQRIVAVNMTGV
EEEECCCCCCCCCCCHHHHHHHHHHHHEEEECCCCCCCCEEEECCCCCCEEEEEEECCCE
LNLSQVFIPNMRQNGGGSIACMSSVSAQRGGGIFGGPHYSAAKAGVLGLAKAMAREFGPD
EEHHHHHCCCCCCCCCCCEEEEECCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
SIRVNCVTPGLIQTDITGDKLSAEMRADIVKGIPLSRLGDARDVANIYLFLASDLSAYVT
CEEEEEECCCEEEECCCCCHHHHHHHHHHHHCCCHHHCCCHHHHHHEEEEHHCCHHHHHE
GAVIDVNGGMLIH
EEEEEECCCEEEC
>Mature Secondary Structure
MLLKGKTAVISGAASKRGIGRATAELFASHGARVAILDINADEAKAAAGDLPPVEHGAHI
CCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHCCCCCCCCCCCCEE
GLRCDVADRASCTSASDEVLSAFGVANILINNAGITQPVKTLDISDADWQRIVAVNMTGV
EEEECCCCCCCCCCCHHHHHHHHHHHHEEEECCCCCCCCEEEECCCCCCEEEEEEECCCE
LNLSQVFIPNMRQNGGGSIACMSSVSAQRGGGIFGGPHYSAAKAGVLGLAKAMAREFGPD
EEHHHHHCCCCCCCCCCCEEEEECCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
SIRVNCVTPGLIQTDITGDKLSAEMRADIVKGIPLSRLGDARDVANIYLFLASDLSAYVT
CEEEEEECCCEEEECCCCCHHHHHHHHHHHHCCCHHHCCCHHHHHHEEEEHHCCHHHHHE
GAVIDVNGGMLIH
EEEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424