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The map label for this gene is ilvG [C]

Identifier: 15668844

GI number: 15668844

Start: 587750

End: 589234

Strand: Direct

Name: ilvG [C]

Synonym: MJ0663

Alternate gene names: 15668844

Gene position: 587750-589234 (Clockwise)

Preceding gene: 15668840

Following gene: 15668845

Centisome position: 35.3

GC content: 31.45

Gene sequence:

>1485_bases
GTGGGTGGTAATATTAAATTCTTAGAAGCTATGGTAGATTTCTTAGAAAGAAATGTAAAAACTATATTTTCCTATCCGGG
AGAGCAGATATTGCCGTTGTATAATGAAATAGAGGGTAGCAGTATAAAAAATATTATGGTTAGGGATGAGAGAGGAGCAG
GGTTCATGGCTGATGGCTATGCAAGAATAACTAACTATATAGGAGTTTGCTTAGCTACTGCAGGTCCTGGAGCTACGAAT
TTAACAACGCCAATAGCAACAGCCTATAAGGATAATTCATCTGTATTGGCAATTACTGGAAGATGTCAGAGAAAATATAT
TGGCAAAAATTATTTTCAAGAGGTAAATATGGATTTTTTAAATTTTTATAAAGGATATTTTGTTGATAAAGCTGAAGTAA
GTTATATAGCAAAAGCATTTGCTGACTGCCTATTTAATAAAAAACCTGTTCAGCTAAATATTCCAGTTGATTTATACAAG
GAAGAAGCAAAAGATATAAATATAACCACATATACAGATATATATAAAGATGATGAAACTCCTTCCAACAACATAAAAGA
AATAGATGTCAAAAAACCACTATTTTTAATTGGGCAGGGTATATTTGGGACTTTAAGTTATAAAGAGATAGTAAAAATAT
CAAAAATATTAGAAAAGATAAACTGTCCCATAGCTACAACATTTCCAGCGAGAGGAGTTATTAATGAAAAATTAGAGAAC
TGCATAGGCTTAGTTGGGAGGAGAGGGGATTTAAAATCTTTGTTAGAGGCAGATAAGATAATAAATATCGGCTCATCTCT
ATCTTACAACACCTATGTAGAGAGTGTTAGGGAAAAGCTTTTAAGTAAAACTGAAAATATACAACTAAAACCAAAAAGCA
TCAAAGAGTTAAAAGAATTTTTTGAAAATTTAGATGTAAAAAATAGTAGCTGGATATATAAAAATAGCAACAAATTTCAG
CCTTCTGGAGATTACTCAAACAAAATCTATGAGATTATTAAAAATATTCCAGAAGATGCCATAATAGTTACAGATGCTGG
TAAGCATACTGTATTTACCTGCTTATTAAAAACCTGCGTTATTCCAAGAAATATCATTTCCTCACACTCATTTGGAACTA
TGGGTTTTGGTTTGCCTGCATCCATTGGAGTAAAATTTGGAACTATAGATTTCAACATCGATAGAGAAGTTGTATTAATT
AGTGGGGATGGCGGATTTTTGATGAATGTTGAGGAGTTGCAAGTTGTAGCTGAAAATAACTTAAAAATCTTAATGGTTGT
GATGAAAAATAACAGTTTGGCAGAGTTCTGCAAGATAAAAAATCCAAACTTCAACAAAATAGCTGATGCATTTGAAATAG
ATAATTGCTACATTGAAAATGTTGATGAAATTGGTTCAGAAATTAAAGGCTATTTAAAAAAGAATAAGCCGTTATTGGTG
GTTGTTGAAACAGAAAACGAGCCATTGCCTAAACCAAATATTTAA

Upstream 100 bases:

>100_bases
ACCACATCAACCCGCCCCATAAGACATAGGCGATAGGATAGATTGCCAATCTAACTAAAATCCTTTTATCCACAATCTCA
CCGTTTAAATCAATATTTTT

Downstream 100 bases:

>100_bases
ATTTAACAATTTAATTTTATAAAATTTGAAAAATTTAGTTTTACGGAGGGATAATATGGGAGAAGTTTTAAATGAATTAT
TTGAAATTTTATCAAAAGAT

Product: acetolactate synthase large subunit IlvB

Products: 2-acetolactate; CO2

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MGGNIKFLEAMVDFLERNVKTIFSYPGEQILPLYNEIEGSSIKNIMVRDERGAGFMADGYARITNYIGVCLATAGPGATN
LTTPIATAYKDNSSVLAITGRCQRKYIGKNYFQEVNMDFLNFYKGYFVDKAEVSYIAKAFADCLFNKKPVQLNIPVDLYK
EEAKDINITTYTDIYKDDETPSNNIKEIDVKKPLFLIGQGIFGTLSYKEIVKISKILEKINCPIATTFPARGVINEKLEN
CIGLVGRRGDLKSLLEADKIINIGSSLSYNTYVESVREKLLSKTENIQLKPKSIKELKEFFENLDVKNSSWIYKNSNKFQ
PSGDYSNKIYEIIKNIPEDAIIVTDAGKHTVFTCLLKTCVIPRNIISSHSFGTMGFGLPASIGVKFGTIDFNIDREVVLI
SGDGGFLMNVEELQVVAENNLKILMVVMKNNSLAEFCKIKNPNFNKIADAFEIDNCYIENVDEIGSEIKGYLKKNKPLLV
VVETENEPLPKPNI

Sequences:

>Translated_494_residues
MGGNIKFLEAMVDFLERNVKTIFSYPGEQILPLYNEIEGSSIKNIMVRDERGAGFMADGYARITNYIGVCLATAGPGATN
LTTPIATAYKDNSSVLAITGRCQRKYIGKNYFQEVNMDFLNFYKGYFVDKAEVSYIAKAFADCLFNKKPVQLNIPVDLYK
EEAKDINITTYTDIYKDDETPSNNIKEIDVKKPLFLIGQGIFGTLSYKEIVKISKILEKINCPIATTFPARGVINEKLEN
CIGLVGRRGDLKSLLEADKIINIGSSLSYNTYVESVREKLLSKTENIQLKPKSIKELKEFFENLDVKNSSWIYKNSNKFQ
PSGDYSNKIYEIIKNIPEDAIIVTDAGKHTVFTCLLKTCVIPRNIISSHSFGTMGFGLPASIGVKFGTIDFNIDREVVLI
SGDGGFLMNVEELQVVAENNLKILMVVMKNNSLAEFCKIKNPNFNKIADAFEIDNCYIENVDEIGSEIKGYLKKNKPLLV
VVETENEPLPKPNI
>Mature_493_residues
GGNIKFLEAMVDFLERNVKTIFSYPGEQILPLYNEIEGSSIKNIMVRDERGAGFMADGYARITNYIGVCLATAGPGATNL
TTPIATAYKDNSSVLAITGRCQRKYIGKNYFQEVNMDFLNFYKGYFVDKAEVSYIAKAFADCLFNKKPVQLNIPVDLYKE
EAKDINITTYTDIYKDDETPSNNIKEIDVKKPLFLIGQGIFGTLSYKEIVKISKILEKINCPIATTFPARGVINEKLENC
IGLVGRRGDLKSLLEADKIINIGSSLSYNTYVESVREKLLSKTENIQLKPKSIKELKEFFENLDVKNSSWIYKNSNKFQP
SGDYSNKIYEIIKNIPEDAIIVTDAGKHTVFTCLLKTCVIPRNIISSHSFGTMGFGLPASIGVKFGTIDFNIDREVVLIS
GDGGFLMNVEELQVVAENNLKILMVVMKNNSLAEFCKIKNPNFNKIADAFEIDNCYIENVDEIGSEIKGYLKKNKPLLVV
VETENEPLPKPNI

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family

Homologues:

Organism=Homo sapiens, GI93004078, Length=545, Percent_Identity=20, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI1790104, Length=536, Percent_Identity=25.9328358208955, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI87081685, Length=535, Percent_Identity=24.1121495327103, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1786717, Length=528, Percent_Identity=24.0530303030303, Blast_Score=113, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323755, Length=576, Percent_Identity=24.1319444444444, Blast_Score=146, Evalue=7e-36,
Organism=Drosophila melanogaster, GI19922626, Length=469, Percent_Identity=21.1087420042644, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y663_METJA (Q58077)

Other databases:

- EMBL:   L77117
- PIR:   G64382
- RefSeq:   NP_247647.1
- ProteinModelPortal:   Q58077
- GeneID:   1451529
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0663
- NMPDR:   fig|243232.1.peg.684
- TIGR:   MJ0663
- HOGENOM:   HBG539102
- OMA:   VVECENE
- ProtClustDB:   CLSK876283
- BioCyc:   MetaCyc:MONOMER-14614
- BioCyc:   MJAN243232:MJ_0663-MONOMER
- InterPro:   IPR012001
- InterPro:   IPR011766

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: 2.2.1.6

Molecular weight: Translated: 55356; Mature: 55224

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGNIKFLEAMVDFLERNVKTIFSYPGEQILPLYNEIEGSSIKNIMVRDERGAGFMADGY
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEEEECCCCCCCCCCCH
ARITNYIGVCLATAGPGATNLTTPIATAYKDNSSVLAITGRCQRKYIGKNYFQEVNMDFL
HHHHHHHHEEEECCCCCCCCCCCCHHEEECCCCCEEEEECCHHHHHHHHHHHHHHCHHHH
NFYKGYFVDKAEVSYIAKAFADCLFNKKPVQLNIPVDLYKEEAKDINITTYTDIYKDDET
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCCCEEEEEEEEECCCCC
PSNNIKEIDVKKPLFLIGQGIFGTLSYKEIVKISKILEKINCPIATTFPARGVINEKLEN
CCCCCEEEECCCCEEEECCCHHCCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHH
CIGLVGRRGDLKSLLEADKIINIGSSLSYNTYVESVREKLLSKTENIQLKPKSIKELKEF
HHHHHCCCCHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHH
FENLDVKNSSWIYKNSNKFQPSGDYSNKIYEIIKNIPEDAIIVTDAGKHTVFTCLLKTCV
HHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHC
IPRNIISSHSFGTMGFGLPASIGVKFGTIDFNIDREVVLISGDGGFLMNVEELQVVAENN
CCHHHHCCCCCCCCCCCCCHHCCEEEEEEEEECCCEEEEEECCCCEEEEHHHHHHEECCC
LKILMVVMKNNSLAEFCKIKNPNFNKIADAFEIDNCYIENVDEIGSEIKGYLKKNKPLLV
CEEEEEEEECCCHHHHHHCCCCCHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHCCCCEEE
VVETENEPLPKPNI
EEECCCCCCCCCCC
>Mature Secondary Structure 
GGNIKFLEAMVDFLERNVKTIFSYPGEQILPLYNEIEGSSIKNIMVRDERGAGFMADGY
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEEEECCCCCCCCCCCH
ARITNYIGVCLATAGPGATNLTTPIATAYKDNSSVLAITGRCQRKYIGKNYFQEVNMDFL
HHHHHHHHEEEECCCCCCCCCCCCHHEEECCCCCEEEEECCHHHHHHHHHHHHHHCHHHH
NFYKGYFVDKAEVSYIAKAFADCLFNKKPVQLNIPVDLYKEEAKDINITTYTDIYKDDET
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCCCEEEEEEEEECCCCC
PSNNIKEIDVKKPLFLIGQGIFGTLSYKEIVKISKILEKINCPIATTFPARGVINEKLEN
CCCCCEEEECCCCEEEECCCHHCCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHH
CIGLVGRRGDLKSLLEADKIINIGSSLSYNTYVESVREKLLSKTENIQLKPKSIKELKEF
HHHHHCCCCHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHH
FENLDVKNSSWIYKNSNKFQPSGDYSNKIYEIIKNIPEDAIIVTDAGKHTVFTCLLKTCV
HHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHC
IPRNIISSHSFGTMGFGLPASIGVKFGTIDFNIDREVVLISGDGGFLMNVEELQVVAENN
CCHHHHCCCCCCCCCCCCCHHCCEEEEEEEEECCCEEEEEECCCCEEEEHHHHHHEECCC
LKILMVVMKNNSLAEFCKIKNPNFNKIADAFEIDNCYIENVDEIGSEIKGYLKKNKPLLV
CEEEEEEEECCCHHHHHHCCCCCHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHCCCCEEE
VVETENEPLPKPNI
EEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: pyruvate

Specific reaction: 2 pyruvate = 2-acetolactate + CO2

General reaction: C-C bond formation; decarboxylation [C]

Inhibitor: @OXOB02.txt*2-oxobutanoate!; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087