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The map label for this gene is mjaIIIM

Identifier: 15668778

GI number: 15668778

Start: 529634

End: 530503

Strand: Direct

Name: mjaIIIM

Synonym: MJ0598

Alternate gene names: 15668778

Gene position: 529634-530503 (Clockwise)

Preceding gene: 15668777

Following gene: 15668779

Centisome position: 31.81

GC content: 27.82

Gene sequence:

>870_bases
ATGGAAGTTAAACCTTTTTTAAAATGGGCTGGAGGAAAAACACAAATTTTAAGTCAAATAGAAGAAAATTTACCAAAAGA
ATTGAAGGAAGGAAACATTAAAAAATATATAGAACCCTTTGTTGGTGGAGGGGCAGTTTTATTTTACCTTTTACAAAAAT
ACGAATTTAAAAAAGTTATTATCAGTGATATTAACGAGGATTTGATGCTATGCTATAAAGTTGTTAAAAATGATGTCGAT
AGATTAATTGAGGAGTTATCATCCTTAAGGGATGAATTTTTATCATTGGATGAAGAAAAAAGGAAAGAATTTTATTATAA
AGTTAGAGATGACTTTAACAAAAACAAGAATGATTGTGATGAAGTTAAAAGAGTGGCACAGTTTATATTTTTAAATAAAA
CATGTTATAATGGGCTATATAGGGTTAATAAAAAAGGAGAATTTAATGTTCCTTATGGAAGGTATAAAAACCCAAAGATT
TTTGATGAACAAAATTTGAAAAATGTTTCAAAATTATTAAAGAACGTTAAAATCCTCTGTGGAGATTTTGAGATTGTTGA
TGAGTATGTTGATGCTGAAAGCTTTGTTTATTTCGATCCACCATATAAGCCGTTAAATAAAACATCTTCCTTCACATCCT
ATACAAAGTATGATTTTAACGATGATGATCAAATTAGATTGGCAAAATTTTATAGAAAATTGGATAAAAGAGGGGCTAAG
TTGATGTTAAGCAACTCTTATAATGTTGATTTCTTTGGAAAGCTTTATGAGGGCTTTAATATAAAAAAGGTTGTTGCTAA
GAGGATGATTAACTGCAAAGGAGATAAAAGAAAAGATGGTATATATGAGTTATTGATTATGAATTATTGA

Upstream 100 bases:

>100_bases
GTTGAGGAGCTTAGAGAAAATATAAATGAGAGAATAGAAAGTTATGTATCTAAAGTTTTAAGGAAGATTAAAGCCAGTTA
ATCTAATTTTTTGGGATAAA

Downstream 100 bases:

>100_bases
ATAATTTTAAGGTGATTTAATGCAAATAAAGCCATTTGAAGAGAATAAGCCCTATCCAGAATGGGATCCAGATGAGCCAG
ATAAAGTCCCAGGAATTCCT

Product: type II R/M system modification methyltransferase

Products: NA

Alternate protein names: M.MjaIII; Adenine-specific methyltransferase MjaIII

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MEVKPFLKWAGGKTQILSQIEENLPKELKEGNIKKYIEPFVGGGAVLFYLLQKYEFKKVIISDINEDLMLCYKVVKNDVD
RLIEELSSLRDEFLSLDEEKRKEFYYKVRDDFNKNKNDCDEVKRVAQFIFLNKTCYNGLYRVNKKGEFNVPYGRYKNPKI
FDEQNLKNVSKLLKNVKILCGDFEIVDEYVDAESFVYFDPPYKPLNKTSSFTSYTKYDFNDDDQIRLAKFYRKLDKRGAK
LMLSNSYNVDFFGKLYEGFNIKKVVAKRMINCKGDKRKDGIYELLIMNY

Sequences:

>Translated_289_residues
MEVKPFLKWAGGKTQILSQIEENLPKELKEGNIKKYIEPFVGGGAVLFYLLQKYEFKKVIISDINEDLMLCYKVVKNDVD
RLIEELSSLRDEFLSLDEEKRKEFYYKVRDDFNKNKNDCDEVKRVAQFIFLNKTCYNGLYRVNKKGEFNVPYGRYKNPKI
FDEQNLKNVSKLLKNVKILCGDFEIVDEYVDAESFVYFDPPYKPLNKTSSFTSYTKYDFNDDDQIRLAKFYRKLDKRGAK
LMLSNSYNVDFFGKLYEGFNIKKVVAKRMINCKGDKRKDGIYELLIMNY
>Mature_289_residues
MEVKPFLKWAGGKTQILSQIEENLPKELKEGNIKKYIEPFVGGGAVLFYLLQKYEFKKVIISDINEDLMLCYKVVKNDVD
RLIEELSSLRDEFLSLDEEKRKEFYYKVRDDFNKNKNDCDEVKRVAQFIFLNKTCYNGLYRVNKKGEFNVPYGRYKNPKI
FDEQNLKNVSKLLKNVKILCGDFEIVDEYVDAESFVYFDPPYKPLNKTSSFTSYTKYDFNDDDQIRLAKFYRKLDKRGAK
LMLSNSYNVDFFGKLYEGFNIKKVVAKRMINCKGDKRKDGIYELLIMNY

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MjaIII endonuclease (Potential)

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family

Homologues:

Organism=Escherichia coli, GI1789789, Length=284, Percent_Identity=35.5633802816901, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTM3_METJA (Q58015)

Other databases:

- EMBL:   L77117
- PIR:   F64374
- RefSeq:   NP_247580.1
- ProteinModelPortal:   Q58015
- SMR:   Q58015
- REBASE:   3890
- GeneID:   1451463
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0598
- NMPDR:   fig|243232.1.peg.617
- TIGR:   MJ0598
- HOGENOM:   HBG586381
- OMA:   KARRTIS
- ProtClustDB:   CLSK2517633
- BioCyc:   MJAN243232:MJ_0598-MONOMER
- BRENDA:   2.1.1.72
- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327
- Gene3D:   G3DSA:1.10.1020.10
- PRINTS:   PR00505
- TIGRFAMs:   TIGR00571

Pfam domain/function: PF02086 MethyltransfD12

EC number: =2.1.1.72

Molecular weight: Translated: 34235; Mature: 34235

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS00092 N6_MTASE

Important sites: BINDING 9-9 BINDING 13-13 BINDING 63-63 BINDING 199-199

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHEEEECC
>Mature Secondary Structure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EEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087