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The map label for this gene is frvX [C]

Identifier: 15668735

GI number: 15668735

Start: 490782

End: 491834

Strand: Direct

Name: frvX [C]

Synonym: MJ0555

Alternate gene names: 15668735

Gene position: 490782-491834 (Clockwise)

Preceding gene: 15668729

Following gene: 15668739

Centisome position: 29.48

GC content: 35.52

Gene sequence:

>1053_bases
ATGTCAGTTGTTGAATACTTAAAAAAGCTCTCAAAACTACATGGAATATCTGGGAGGGAGGATAGTGTTAGAGAGTTTAT
GAAAAAAGAGTTGGAAAAATACTGTGATTCCGTTGAAATTGATAACTTTGGGAATTTAATAGCAAAGAGAGGGAATAAAG
GAAAGAAGATTATGATAGCAGCTCACATGGATGAGATTGGTTTGATGGTTAAATATATTGACGATAATGGTTTCTTAAAA
TTCACAAAGATTGGAGGCATTTATGACCCAACAATATTAAACCAAAAGGTTGTTGTTCATGGAAGTAAAGGGGATTTAAT
TGGTGTTTTAGGCTCAAAACCTCCACACAGAATGAAAGAAGAAGAAAAAACCAAAATAATTAAATATGAAGATATGTTTA
TAGACATTGGAGCTGAGAGTAGGGAAGAGGCTATAGAAATGGGGGTTAATATAGGAACATGGGTTTCTTTTTTAAGTGAG
GTTTATGATTTAGGTAAAAATAGATTAACTGGAAAGGCATTTGATGATAGGGTGGGATGTGCTGTTCTCTTAGAAGTTAT
GAAAAGGTTATCTGAAGAAGATATTGACTGTCAGGTCTATGCAGTGGGAACTGTCCAAGAGGAAGTTGGATTAAAAGGGG
CAAGAGTCTCTGCCTTTAAAATAAATCCAGATGTTGCCATTGCGTTAGATGTTACTATAGCAGGAGACCATCCAGGAATT
AAAAAAGAAGATGCTCCGGTAGATTTAGGTAAGGGGCCAGTGGTTGGAATAGTAGATGCATCTGGTAGAGGGTTGATAGC
TCATCCAAAAGTCTTAGATATGATTAAAGCTGTTTCTGAAAAGTATAAAATAGATGTTCAGTGGGAAGTTGGTGAGGGAG
GAACTACAGATGCAACAGCCATTCATTTAACAAGAGAGGGAATTCCAACTGGTGTTATCTCTGTTCCAGCAAGATATATA
CATACTCCAGTTGAAGTTATTGATAAGAGAGATTTAGAAAAAACTGTGGAATTGGTTTATAATTGCATAAAAGAGGTCAA
TAATTTCTTCTAA

Upstream 100 bases:

>100_bases
AAAATTGACGAAAGGAAATTGGACACTTACCATTAAAACATAAATCTAAAATAGTTGGATTAGGAATACATTAACTACTA
TTTAAAAAAGGTGAGAGATT

Downstream 100 bases:

>100_bases
AAGTATTTTTAATTATTCTAAGTTCTTTTTTTAGCTTTAATATTTTATCTTAATCTCAGTATTGTGATTGTAGAAACTTA
CAAGAGTTTTTAACTCTTTT

Product: endoglucanase CelM

Products: D-glucose; GDP

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MSVVEYLKKLSKLHGISGREDSVREFMKKELEKYCDSVEIDNFGNLIAKRGNKGKKIMIAAHMDEIGLMVKYIDDNGFLK
FTKIGGIYDPTILNQKVVVHGSKGDLIGVLGSKPPHRMKEEEKTKIIKYEDMFIDIGAESREEAIEMGVNIGTWVSFLSE
VYDLGKNRLTGKAFDDRVGCAVLLEVMKRLSEEDIDCQVYAVGTVQEEVGLKGARVSAFKINPDVAIALDVTIAGDHPGI
KKEDAPVDLGKGPVVGIVDASGRGLIAHPKVLDMIKAVSEKYKIDVQWEVGEGGTTDATAIHLTREGIPTGVISVPARYI
HTPVEVIDKRDLEKTVELVYNCIKEVNNFF

Sequences:

>Translated_350_residues
MSVVEYLKKLSKLHGISGREDSVREFMKKELEKYCDSVEIDNFGNLIAKRGNKGKKIMIAAHMDEIGLMVKYIDDNGFLK
FTKIGGIYDPTILNQKVVVHGSKGDLIGVLGSKPPHRMKEEEKTKIIKYEDMFIDIGAESREEAIEMGVNIGTWVSFLSE
VYDLGKNRLTGKAFDDRVGCAVLLEVMKRLSEEDIDCQVYAVGTVQEEVGLKGARVSAFKINPDVAIALDVTIAGDHPGI
KKEDAPVDLGKGPVVGIVDASGRGLIAHPKVLDMIKAVSEKYKIDVQWEVGEGGTTDATAIHLTREGIPTGVISVPARYI
HTPVEVIDKRDLEKTVELVYNCIKEVNNFF
>Mature_349_residues
SVVEYLKKLSKLHGISGREDSVREFMKKELEKYCDSVEIDNFGNLIAKRGNKGKKIMIAAHMDEIGLMVKYIDDNGFLKF
TKIGGIYDPTILNQKVVVHGSKGDLIGVLGSKPPHRMKEEEKTKIIKYEDMFIDIGAESREEAIEMGVNIGTWVSFLSEV
YDLGKNRLTGKAFDDRVGCAVLLEVMKRLSEEDIDCQVYAVGTVQEEVGLKGARVSAFKINPDVAIALDVTIAGDHPGIK
KEDAPVDLGKGPVVGIVDASGRGLIAHPKVLDMIKAVSEKYKIDVQWEVGEGGTTDATAIHLTREGIPTGVISVPARYIH
TPVEVIDKRDLEKTVELVYNCIKEVNNFF

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family

Homologues:

Organism=Escherichia coli, GI1790332, Length=337, Percent_Identity=36.7952522255193, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI87082417, Length=346, Percent_Identity=34.6820809248555, Blast_Score=217, Evalue=7e-58,
Organism=Escherichia coli, GI1788727, Length=364, Percent_Identity=29.6703296703297, Blast_Score=162, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y555_METJA (Q57975)

Other databases:

- EMBL:   L77117
- PIR:   C64369
- RefSeq:   NP_247534.1
- ProteinModelPortal:   Q57975
- GeneID:   1451420
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0555
- NMPDR:   fig|243232.1.peg.571
- TIGR:   MJ0555
- HOGENOM:   HBG426188
- OMA:   LEKYCDS
- ProtClustDB:   CLSK876246
- BioCyc:   MJAN243232:MJ_0555-MONOMER
- InterPro:   IPR008007
- PIRSF:   PIRSF001123

Pfam domain/function: PF05343 Peptidase_M42

EC number: 3.2.1.4

Molecular weight: Translated: 38622; Mature: 38491

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: ACT_SITE 207-207

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVEYLKKLSKLHGISGREDSVREFMKKELEKYCDSVEIDNFGNLIAKRGNKGKKIMIA
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEEEE
AHMDEIGLMVKYIDDNGFLKFTKIGGIYDPTILNQKVVVHGSKGDLIGVLGSKPPHRMKE
EECCCCCEEEEEECCCCCEEEEECCCEECCHHCCCEEEEECCCCCEEEECCCCCCHHCCH
EEKTKIIKYEDMFIDIGAESREEAIEMGVNIGTWVSFLSEVYDLGKNRLTGKAFDDRVGC
HHHHEEEEEEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
AVLLEVMKRLSEEDIDCQVYAVGTVQEEVGLKGARVSAFKINPDVAIALDVTIAGDHPGI
HHHHHHHHHHCCCCCCEEEEEEECCHHHHCCCCCEEEEEEECCCEEEEEEEEEECCCCCC
KKEDAPVDLGKGPVVGIVDASGRGLIAHPKVLDMIKAVSEKYKIDVQWEVGEGGTTDATA
CCCCCCCCCCCCCEEEEEECCCCCEEECHHHHHHHHHHHHHEEEEEEEECCCCCCCCCEE
IHLTREGIPTGVISVPARYIHTPVEVIDKRDLEKTVELVYNCIKEVNNFF
EEEECCCCCCCHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVVEYLKKLSKLHGISGREDSVREFMKKELEKYCDSVEIDNFGNLIAKRGNKGKKIMIA
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEEEE
AHMDEIGLMVKYIDDNGFLKFTKIGGIYDPTILNQKVVVHGSKGDLIGVLGSKPPHRMKE
EECCCCCEEEEEECCCCCEEEEECCCEECCHHCCCEEEEECCCCCEEEECCCCCCHHCCH
EEKTKIIKYEDMFIDIGAESREEAIEMGVNIGTWVSFLSEVYDLGKNRLTGKAFDDRVGC
HHHHEEEEEEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
AVLLEVMKRLSEEDIDCQVYAVGTVQEEVGLKGARVSAFKINPDVAIALDVTIAGDHPGI
HHHHHHHHHHCCCCCCEEEEEEECCHHHHCCCCCEEEEEEECCCEEEEEEEEEECCCCCC
KKEDAPVDLGKGPVVGIVDASGRGLIAHPKVLDMIKAVSEKYKIDVQWEVGEGGTTDATA
CCCCCCCCCCCCCEEEEEECCCCCEEECHHHHHHHHHHHHHEEEEEEEECCCCCCCCCEE
IHLTREGIPTGVISVPARYIHTPVEVIDKRDLEKTVELVYNCIKEVNNFF
EEEECCCCCCCHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPglucose; H2O

Specific reaction: GDP-glucose + H2O = D-glucose + GDP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087