Click here to switch to the map view.

The map label for this gene is thyA

Identifier: 15668688

GI number: 15668688

Start: 452282

End: 452950

Strand: Reverse

Name: thyA

Synonym: MJ0511

Alternate gene names: 15668688

Gene position: 452950-452282 (Counterclockwise)

Preceding gene: 15668689

Following gene: 15668687

Centisome position: 27.2

GC content: 31.99

Gene sequence:

>669_bases
ATGCTATGTATAAAAAAGCCTTCTGTAGCCTCAGCTTTTAATGAATTAATTCCCAAAATATTGAAAGATGGCGAAGTAGT
TGAGACAGAGTTTGAAGAGAGGACTAAAGAAATTAGAAATACGATTATAGAAATTACAAATCCAAAATTAAAAAAAGTTC
CAGAAAAATATCCGTTGGGAGAGAAAGCTGTGGAAGAATACACAAAAAATCTTTTATATGGCTCTAAAAATGTTTTCAGC
TATGATTATCATCAGAGGTTGTTTGAATATCCTTATGCTGATGAAAAAATTAACCAAATAGATTATATTATTGAAAAATT
AAATCAACAAAAAAATAGTAGGAGAGCTGTAGCAATTACTTGGAATCCAAAAATTGATATTGAGGTTAGTAGGGATGAAA
GAGGAAGCGTCCCTTGTTTGCAACTTGTTCAATTCTTAATTAGAAATGGGAAACTGTATCAAACTGTTATCTTCAGAAGC
AATGACGCACTACTGGCTTTCGTAAGTAATGCGATAGGTCTGATAACGTTGGGAGAGTATATAGCAAAAAAGGTTGGCGT
TGGTTATGGTACTTATACTCATCATGCTATTTCAATGCATATTTATGTTGACCGGGATTTTGACTATATTAAAAAATACT
TCCCTGAATGTTTGAAATATTTGTGGTGA

Upstream 100 bases:

>100_bases
TGAAATGGAAGATATTTAAATCTTTTTTATATTTCATCAAATTTTTATACTTGTTGTATCTATTTTTTATCATAATTAAA
TACAATTACAAAGGGAGAGT

Downstream 100 bases:

>100_bases
TTGAAAATGGGTGTTAGGAAAAAGGTAAGTGACAGAATGATTAATGAATTTATCAGATTATATTTAGACGAGAAGTGATC
TATTTCGGAAATAGCAAATT

Product: thymidylate synthase

Products: dihydrofolate; dTMP

Alternate protein names: TS; TSase

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MLCIKKPSVASAFNELIPKILKDGEVVETEFEERTKEIRNTIIEITNPKLKKVPEKYPLGEKAVEEYTKNLLYGSKNVFS
YDYHQRLFEYPYADEKINQIDYIIEKLNQQKNSRRAVAITWNPKIDIEVSRDERGSVPCLQLVQFLIRNGKLYQTVIFRS
NDALLAFVSNAIGLITLGEYIAKKVGVGYGTYTHHAISMHIYVDRDFDYIKKYFPECLKYLW

Sequences:

>Translated_222_residues
MLCIKKPSVASAFNELIPKILKDGEVVETEFEERTKEIRNTIIEITNPKLKKVPEKYPLGEKAVEEYTKNLLYGSKNVFS
YDYHQRLFEYPYADEKINQIDYIIEKLNQQKNSRRAVAITWNPKIDIEVSRDERGSVPCLQLVQFLIRNGKLYQTVIFRS
NDALLAFVSNAIGLITLGEYIAKKVGVGYGTYTHHAISMHIYVDRDFDYIKKYFPECLKYLW
>Mature_222_residues
MLCIKKPSVASAFNELIPKILKDGEVVETEFEERTKEIRNTIIEITNPKLKKVPEKYPLGEKAVEEYTKNLLYGSKNVFS
YDYHQRLFEYPYADEKINQIDYIIEKLNQQKNSRRAVAITWNPKIDIEVSRDERGSVPCLQLVQFLIRNGKLYQTVIFRS
NDALLAFVSNAIGLITLGEYIAKKVGVGYGTYTHHAISMHIYVDRDFDYIKKYFPECLKYLW

Specific function: May catalyze the biosynthesis of dTMP using an unknown cosubstrate

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Archaeal- type thyA subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TYSY_METJA (Q57931)

Other databases:

- EMBL:   L77117
- PIR:   G64363
- RefSeq:   NP_247487.1
- ProteinModelPortal:   Q57931
- GeneID:   1451373
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0511
- NMPDR:   fig|243232.1.peg.524
- TIGR:   MJ0511
- HOGENOM:   HBG540931
- OMA:   HHSVSMH
- ProtClustDB:   PRK00956
- BioCyc:   MJAN243232:MJ_0511-MONOMER
- BRENDA:   2.1.1.45
- GO:   GO:0005737
- HAMAP:   MF_01686
- InterPro:   IPR000398
- InterPro:   IPR014620
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PIRSF:   PIRSF036752
- TIGRFAMs:   TIGR03283

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: 2.1.1.45

Molecular weight: Translated: 25933; Mature: 25933

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 139-139

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCIKKPSVASAFNELIPKILKDGEVVETEFEERTKEIRNTIIEITNPKLKKVPEKYPLG
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCC
EKAVEEYTKNLLYGSKNVFSYDYHQRLFEYPYADEKINQIDYIIEKLNQQKNSRRAVAIT
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
WNPKIDIEVSRDERGSVPCLQLVQFLIRNGKLYQTVIFRSNDALLAFVSNAIGLITLGEY
ECCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHH
IAKKVGVGYGTYTHHAISMHIYVDRDFDYIKKYFPECLKYLW
HHHHHCCCCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MLCIKKPSVASAFNELIPKILKDGEVVETEFEERTKEIRNTIIEITNPKLKKVPEKYPLG
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCC
EKAVEEYTKNLLYGSKNVFSYDYHQRLFEYPYADEKINQIDYIIEKLNQQKNSRRAVAIT
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
WNPKIDIEVSRDERGSVPCLQLVQFLIRNGKLYQTVIFRSNDALLAFVSNAIGLITLGEY
ECCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHH
IAKKVGVGYGTYTHHAISMHIYVDRDFDYIKKYFPECLKYLW
HHHHHCCCCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5,10-methylenetetrahydrofolate; dUMP

Specific reaction: 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087